1. Peroxidase-Like Activity of Silver Nanoparticles Loaded Filter Paper and its Potential Application for Sensing
- Author
-
Kuda Feke and Melisew Tadele Alula
- Subjects
Filter paper ,Thiocyanate ,Chemistry ,Nanochemistry ,General Chemistry ,Condensed Matter Physics ,Biochemistry ,Silver nanoparticle ,Catalysis ,Nanomaterials ,symbols.namesake ,chemistry.chemical_compound ,symbols ,General Materials Science ,Raman spectroscopy ,Hydrogen peroxide ,Nuclear chemistry - Abstract
Nanozymes are nanomaterials that possess intrinsic enzyme mimicking activity. In this study, the application of silver nanoparticles coated filter paper (AgNPs/FP) that exhibits a peroxidase-like catalytic activity as a potential sensor is reported. A simple chemical reduction method was used to produce AgNPs/FP. The formation of the particles on filter paper were characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), and surface-enhanced Raman spectroscopy (SERS). In the presence of hydrogen peroxide, AgNPs/FP oxidized the colorless o-phenylenediamine (OPD) and 3,3′,5,5′-tetramethylbenzidine (TMB) solutions to a yellow colored 2,3-diaminophenazine (DAP) and deep blue colored oxidized TMB (oxTMB), respectively. In the presence of thiocyanate, however, the peroxidase-like activity was inhibited in a concentration dependent manner. This makes the AgNPs/FP a promising catalyst and employed for colorimetric detection of thiocyanate via inhibition of the peroxidase-like activity. The application can be further extended to other environmentally and biologically important analytes.
- Published
- 2021