Objective To analyze the pathogen strains and drug resistance of 862 patients with nosocomial infection, and to provide scientific data for the control of nosocomial infection. Methods Samples of respiratory tract, urinary tract and blood were collected from 862 patients with nosocomial infection in our hospital, and strain culture and AGAR paper diffusion were used to detect drug resistance. Results The infection sites of 862 patients mainly included 58 cases of upper respiratory tract infection, 304 cases of lower respiratory tract infection, 121 cases of bacteremia, 150 cases of intrapelvic tissue infection, 36 cases of ascites infection, and 76 cases of urinary tract infection. A total of 582 strains of pathogenic bacteria were isolated from 862 patients. Among the 582 pathogenic bacteria, there were 400 strains of gram-negative bacteria, including 96 strains of Klebsiella pneumoniae, 105 strains of Escherichia coli and 75 strains of Acinetobacter baumannii; there were 148 gram-positive strains, including 35 strains of Staphylococcus aureus and 58 strains of Coagulasenegative staphylococci; there were 32 strains of fungi, mainly including 16 strains of Candida albicans, and 2 strains of Mycoplasma. Gram-negative bacteria had higher resistance to cefuroxime and ampicillin, and lower drug resistance rate to imipenem, meropenem, tegacycline and polymyxin B. The drug resistance rates of Escherichia coli and Klebsiella pneumoniae to cefuroxime, ampicillin, ciprofloxacin and minocycline were high, all of which were close to or > 30%. The drug resistance rate of pseudomonas aeruginosa to ticacillin, gentamicin, minocycline and furantoin was high, with the drug resistance rate of > 30%. Acinetobacter baumannii was extensively resistant to most commonly used clinical antibiotics, and had a low resistance rate to minocycline, cefoperazone sulbactam, tegacycline and polymyxin B. The resistance rate of Gram-positive bacteria to ampicillin, erythromycin and ciprofloxacin was higher than that of Gram-positive bacteria. There were 11 strains of methicillin-sensitive staphylococcus aureus (MSSA), 24 strains of methicillin-resistant staphylococcus aureus (MRSA), 13 strains of methicillin-positive staphylococcus aureus (MSCNS), and 45 strains of methicillin-negative staphylococcus aureus (MR CNS). MSSA had a high resistance rate to penicillin, erythromycin and tetracycline, MSC NS was sensitive to vancomycin, tigacycline and linezolid, and the resistance rate to erythromycin and gentamicin was > 40%. The resistance rate of MRSA to erythromycin, ciprofloxacin, tetracycline, clindamycin and trimethoprim was all > 50%, and the resistance rate to vancomycin, tegacycline, linezolid, furantoin and rifampin was all < 30%. The resistance rate of MRCNS to vancomycin, tigacycline, linezolid, furantoin and rifampicin were all <30%. Conclusions The main pathogenic bacteria are Gram-negative bacteria, mainly Klebsiella pneumoniae and Escherichia coli. The resistance to cefuroxime, ampicillin and other drugs is relatively high, while the resistance to imipenem is relatively low. The Gram-positive bacteria are mainly Staphylococcus aureus and Coagulase-negative staphylococci, which have higher resistance to ampicillin, erythromycin and ciprofloxacin, and lower resistance to vancomycin, linezolid and tegacycline. [ABSTRACT FROM AUTHOR]