1. Hydrolytic and oxidative enzyme production through cultivation of Pleurotus ostreatus on pulp and paper industry wastes.
- Author
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Skočaj, Matej, Gregori, Andrej, Grundner, Maja, Sepčić, Kristina, and Sežun, Mija
- Subjects
CELLULASE ,ENZYMES ,LIPASES ,PAPER ,PLEUROTUS ostreatus ,PEROXIDASE ,XYLANASES - Abstract
The growth of oyster mushroom (Pleurotus ostreatus) on pulp and paper industry wastes was studied. Specifically, the question was investigated whether solid-state fermentation of P. ostreatus on paper-mill deinking sludge and primary sludge substrates is appropriate for production of enzymes, relevant to the pulp and paper industry. Following fermentation, extracellular protein was extracted and the specific activities of four enzymes were determined, namely, the cellulase, xylanase, lipase and peroxidase. Furthermore, the effects of the pH of the extraction buffer on these enzyme activities were determined, along with the effects of the incubation time. The data show that P. ostreatus can grow on solid wastes from the pulp and paper industry, which could help to minimize the waste volume and to decrease the ecological impact. Furthermore, the solid wastes in focus are good substrates for the production of commercially interesting enzymes. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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