36,506 results
Search Results
2. 40th Anniversary Issue: Reflections on papers from the archive on "Mechanobiology".
- Author
-
Black RA and Houston G
- Subjects
- Biomechanical Phenomena, Anniversaries and Special Events, Cell Biology, Mechanical Phenomena, Publications
- Published
- 2019
- Full Text
- View/download PDF
3. Sanofi-Cell Research outstanding paper award of 2017.
- Author
-
Office E
- Subjects
- Cell Biology history, History, 21st Century, Research Report history, Research Report standards, Awards and Prizes, Cell Biology standards
- Published
- 2018
- Full Text
- View/download PDF
4. Animal Contributed Papers.
- Subjects
- Animals, Cell Biology trends, Cytological Techniques trends, In Vitro Techniques trends
- Published
- 2018
- Full Text
- View/download PDF
5. Plant Contributed Papers.
- Subjects
- Cell Biology trends, Plant Physiological Phenomena
- Published
- 2018
- Full Text
- View/download PDF
6. Sanofi-Cell Research outstanding paper award of 2015.
- Subjects
- Animals, History, 21st Century, Mice, Awards and Prizes, Cell Biology history, Drug Industry economics, Research economics, Research standards
- Published
- 2016
- Full Text
- View/download PDF
7. Sanofi-Cell Research outstanding paper award of 2014.
- Author
-
Li D
- Subjects
- Animals, Research Report, Awards and Prizes, Biomedical Research, Cell Biology
- Published
- 2015
- Full Text
- View/download PDF
8. An Investigation of the Impact of Haptics for Promoting Understanding of Difficult Concepts in Cell Biology
- Author
-
Webb, Mary, Tracey, Megan, Harwin, William, Tokatli, Ozan, Hwang, Faustina, Barrett, Natasha, Jones, Chris, Johnson, Ros, Rannenberg, Kai, Editor-in-Chief, Sakarovitch, Jacques, Editorial Board Member, Goedicke, Michael, Editorial Board Member, Tatnall, Arthur, Editorial Board Member, Neuhold, Erich J., Editorial Board Member, Pras, Aiko, Editorial Board Member, Tröltzsch, Fredi, Editorial Board Member, Pries-Heje, Jan, Editorial Board Member, Kreps, David, Editorial Board Member, Reis, Ricardo, Editorial Board Member, Furnell, Steven, Editorial Board Member, Furbach, Ulrich, Editorial Board Member, Winckler, Marco, Editorial Board Member, Malaka, Rainer, Editorial Board Member, Passey, Don, editor, Bottino, Rosa, editor, Lewin, Cathy, editor, and Sanchez, Eric, editor
- Published
- 2019
- Full Text
- View/download PDF
9. Constraint-based reasoning in cell biology: on the explanatory role of context.
- Author
-
Matlin KS and Green S
- Subjects
- History, 20th Century, Cell Biology history
- Abstract
Cell biologists, including those seeking molecular mechanistic explanations of cellular phenomena, frequently rely on experimental strategies focused on identifying the cellular context relevant to their investigations. We suggest that such practices can be understood as a guided decomposition strategy, where molecular explanations of phenomena are defined in relation to natural contextual (cell) boundaries. This "top-down" strategy contrasts with "bottom-up" reductionist approaches where well-defined molecular structures and activities are orphaned by their displacement from actual biological functions. We focus on the central role of microscopic imaging in cell biology to uncover possible constraints on the system. We show how identified constraints are used heuristically to limit possible mechanistic explanations to those that are biologically meaningful. Historical examples of this process described here include discovery of the mechanism of oxidative phosphorylation in mitochondria, molecular explanation of the first steps in protein secretion, and identification of molecular motors. We suggest that these instances are examples of a form of downward causation or, more specifically, constraining relations, where higher-level structures and variables delimit and enable lower-level system states. The guided decomposition strategy in our historical cases illustrates the irreducibility of experimentally identified constraints in explaining biological activities of cells. Rather than viewing decomposition and recomposition as separate epistemic activities, we contend that they need to be iteratively integrated to account for the ontological complexity of multi-level systems., (© 2024. Springer Nature Switzerland AG.)
- Published
- 2024
- Full Text
- View/download PDF
10. Analysis of the Internal Hypoxic Environment in Solid Tumor Tissue Using a Folding Paper System
- Author
-
Chia-Hao Huang, Kin Fong Lei, and Kowit-Yu Chong
- Subjects
Paper ,Vascular Endothelial Growth Factor A ,Cell signaling ,Materials science ,Angiogenesis ,Mice, Nude ,Metastasis ,Cell Movement ,Cell Line, Tumor ,Neoplasms ,Human Umbilical Vein Endothelial Cells ,medicine ,Animals ,Humans ,General Materials Science ,Hypoxia ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Mice, Inbred BALB C ,Neovascularization, Pathologic ,Hypoxia (medical) ,Hypoxia-Inducible Factor 1, alpha Subunit ,medicine.disease ,Cell biology ,Oxygen tension ,Vascular endothelial growth factor A ,Cancer cell ,Heterografts ,Female ,medicine.symptom - Abstract
Hypoxia is a nonphysiological oxygen tension which is common in most malignant tumors. Hypoxia stimulates complicated cell signaling networks in cancer cells, e.g., the HIF, PI3K, MAPK, and NFκB pathways. Then, cells release a number of cytokines such as VEGFA to promote the growth of peripheral blood vessels and lead to metastasis. In the current work, understanding of the internal hypoxic environment in solid tumor tissue was attempted by developing a folding paper system. A paper-based solid tumor was constructed by folding a filter paper cultured with cancer cells. The cellular response in each layer could be analyzed by disassembling the folded paper after the culture course. The result showed that an internal hypoxic environment was successfully reproduced in the paper-based solid tumor. The cells in the inner layer expressed high levels of HIF1-α and VEGFA. Hence, proliferation and migration of endothelial cells were shown to be induced by the cells located in the internal hypoxic environment. Moreover, the paper-based solid tumor was transplanted into nude mice for the study of hypoxic response and angiogenesis. The crosstalk between internal and external parts of solid tumor tissue could be analyzed by sectioning each layer of the paper-based solid tumor. This approach provides a favorable analytical method for the discovery of the interaction between cancer cells, hypoxia, and peripheral angiogenesis.
- Published
- 2021
11. Rock, scissors, paper: How RNA structure informs function
- Author
-
Sarah M Assmann, Hong-Li Chou, and Philip C Bevilacqua
- Subjects
Cell Biology ,Plant Science - Abstract
RNA can fold back on itself to adopt a wide range of structures. These range from relatively simple hairpins to intricate 3D folds and can be accompanied by regulatory interactions with both metabolites and macromolecules. The last 50 yr have witnessed elucidation of an astonishing array of RNA structures including transfer RNAs, ribozymes, riboswitches, the ribosome, the spliceosome, and most recently entire RNA structuromes. These advances in RNA structural biology have deepened insight into fundamental biological processes including gene editing, transcription, translation, and structure-based detection and response to temperature and other environmental signals. These discoveries reveal that RNA can be relatively static, like a rock; that it can have catalytic functions of cutting bonds, like scissors; and that it can adopt myriad functional shapes, like paper. We relate these extraordinary discoveries in the biology of RNA structure to the plant way of life. We trace plant-specific discovery of ribozymes and riboswitches, alternative splicing, organellar ribosomes, thermometers, whole-transcriptome structuromes and pan-structuromes, and conclude that plants have a special set of RNA structures that confer unique types of gene regulation. We finish with a consideration of future directions for the RNA structure–function field.
- Published
- 2023
12. Micro-nanofibrillated cellulose preparation from bleached softwood pulp using chemo-refining approach and its evaluation as strength enhancer for paper properties
- Author
-
Puneet Pathak, Nishi Kant Bhardwaj, and Varun Kumar
- Subjects
Softwood ,Materials Science (miscellaneous) ,Sodium ,Sodium chlorite ,chemistry.chemical_element ,02 engineering and technology ,engineering.material ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Hardwood ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,Fourier transform infrared spectroscopy ,Cellulose ,Pulp (paper) ,Papermaking ,Cell Biology ,021001 nanoscience & nanotechnology ,Pulp and paper industry ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,chemistry ,engineering ,0210 nano-technology ,Biotechnology - Abstract
An industry compatible chemo-refining approach was tested for preparation of micro-nanofibrillated cellulose (MNFC) from bleached softwood pulp using sodium meta-periodate and sodium chlorite as oxidizers followed by refining in Valley beater. SEM and FTIR analyses confirmed micro-nano scale fibrillation and chemical functional group modification in laboratory prepared MNFC, respectively. The water retention value, carboxyl content and viscosity of MNFC were found comparable with imported NFC as reference (R-NFC). To evaluate MNFC as strength enhancer for paper properties, 5% MNFC addition to bleached mixed hardwood pulp showed 6% reduction in bulk with 36%, 24% and 97% increment in breaking length, burst index and double fold of the handsheets, respectively without affecting tear index and optical properties than the control. Surface properties were also improved. Pulp drainability (37°SR) after MNFC addition was found suitable for papermaking. These laboratory results confirmed the potential of MNFC as a suitable strength additive for paper quality improvement.
- Published
- 2020
13. Paper-Based Cell Culture: Paving the Pathway for Liver Tissue Model Development on a Cellulose Paper Chip
- Author
-
Mimi R. Borrelli, Tapas K. Maiti, Milad Ashrafizadeh, Tarun Agarwal, and Pooyan Makvandi
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,education ,Biochemistry (medical) ,Biomedical Engineering ,General Chemistry ,Paper based ,Chip ,humanities ,Cell biology ,Biomaterials ,chemistry.chemical_compound ,chemistry ,Cell culture ,Liver tissue ,Model development ,Cellulose - Abstract
The present review provides a comprehensive outlook toward the possibilities of developing a functional in vitro liver tissue model on a paper platform. To this end, we first addressed the suitabil...
- Published
- 2022
14. Call for Papers: 'Targeting Airway Immunity in Lung Disease'
- Author
-
Nathan W. Bartlett, Carol Feghali-Bostwick, and Susan J. Gunst
- Subjects
Pulmonary and Respiratory Medicine ,Physiology ,Physiology (medical) ,Cell Biology - Published
- 2023
15. Preparation and application of nanocellulose from non-wood plants to improve the quality of paper and cardboard
- Author
-
O. V. Yashchenko and V. A. Barbash
- Subjects
Materials science ,Materials Science (miscellaneous) ,Organosolv ,Corrugated fiberboard ,02 engineering and technology ,engineering.material ,010402 general chemistry ,01 natural sciences ,Nanocellulose ,chemistry.chemical_compound ,Lignin ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,biology ,Pulp (paper) ,cardboard ,Cell Biology ,021001 nanoscience & nanotechnology ,biology.organism_classification ,Pulp and paper industry ,Environmentally friendly ,Atomic and Molecular Physics, and Optics ,Kenaf ,0104 chemical sciences ,chemistry ,visual_art ,visual_art.visual_art_medium ,engineering ,0210 nano-technology ,Biotechnology - Abstract
The study describes the preparation of pulp and nanocellulose from non-wood plant materials, as well as an improved properties of paper and cardboard for mass production. The pulps from wheat straw, kenaf and flax fibers were prepared by the environmentally friendly organosolv method—cooking in a solution of isobutanol or peracetic acid. The organosolv pulps used to prepare nanocellulose had traces of lignin and mineral substances. The process of hydrolysis of the investigated organosolv pulps was optimal when carried out under the following conditions: 43% sulfuric acid, temperature 60 °C, hydrolysis time 90 min and ultrasonic treatment 60 min. Using the methods of SEM, XRD, TEM, AFM and TGA, the structure and properties of organosolv pulps and nanocellulose were studied. The use of nanocellulose in bulk and on the surface of mass types of paper and cardboard—paper for corrugation, offset paper, recycled containerboard and cardboard for flat layers of corrugated cardboard were investigated. We established the positive effect of nanocellulose application on the physical and mechanical properties of paper and cardboard. Low consumption of nanocellulose allows production of the paper and cardboard with properties that meet the requirements to appropriate standards and replacement of synthetic reinforcing materials.
- Published
- 2020
16. Fungal melanins that deteriorate paper cultural heritage: An overview
- Author
-
Mario Carlos Nazareno Saparrat, Daniela Silvana Nitiu, and Andrea Cecilia Mallo
- Subjects
Paper ,0106 biological sciences ,Physiology ,media_common.quotation_subject ,Biology ,010603 evolutionary biology ,01 natural sciences ,030308 mycology & parasitology ,03 medical and health sciences ,Fungal Structures ,Genetics ,Fungal colonization ,Library Materials ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,media_common ,Melanins ,0303 health sciences ,Museums ,Foxing ,Fungi ,Environmental ethics ,Pigments, Biological ,Cell Biology ,General Medicine ,Cultural heritage ,Art ,Diversity (politics) - Abstract
Paper-based works of art and documents of cultural importance kept in museums and libraries can show notorious signs of deterioration, including foxing stains, caused by fungal colonization. Some of the main chromophore agents of fungal origin that deteriorate paper and therefore affect paper cultural heritage both aesthetically and structurally are the group of pigments called melanins. Thus, knowledge of the diversity and features of fungal melanins and of the melanization pathways of fungi growing on paper is key to removing these pigments from paper-based works of cultural importance. This review provides an approach about the current knowledge of melanins synthesized by paper-colonizing fungi, their localization in the fungal structures, and their role in the deterioration of paper. This knowledge might contribute to developing new, effective, and sustainable strategies of restoration and conservation of historical documents and works of art based on paper.
- Published
- 2020
17. Facile fabrication of superhydrophobic paper with durability, chemical stability and self-cleaning by roll coating with modified nano-TiO2
- Author
-
Yufeng Wang, Yunzhi Chen, Baoying Shi, Yuhong Teng, Ziyan Li, and Weiwei Fan
- Subjects
Materials science ,Materials Science (miscellaneous) ,02 engineering and technology ,engineering.material ,010402 general chemistry ,01 natural sciences ,Contact angle ,Coating ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,Composite material ,Sandpaper ,Filter paper ,Cell Biology ,Epoxy ,021001 nanoscience & nanotechnology ,Atomic and Molecular Physics, and Optics ,Surface energy ,0104 chemical sciences ,visual_art ,engineering ,visual_art.visual_art_medium ,Adhesive ,0210 nano-technology ,Layer (electronics) ,Biotechnology - Abstract
A superhydrophobic paper with excellent robustness was fabricated by roll coating with modified nano-TiO2. First, nano-TiO2 particles were hydrophobically modified by γ-aminopropyltriethoxysilane and 1H, 1H, 2H, 2H-perfluorooctyltriethoxysilane (POTS). Then the paper coating composed of modified nano-TiO2 particles as pigments and epoxy resin (EP) as adhesives was coated on a paper surface to create the desired surface morphology and surface energy. Compared with the uncoated filter paper, the prepared paper showed an improved rough surface morphology owing to the uniform layer of TiO2 microclusters deposited on the fiber network. The superhydrophobic paper exhibited water contact angles (WCA) about 153° ± 1.5°, and water sliding angles (WSA) about 3.5° ± 0.5°, low surface adhesion and excellent bounce ability. The superhydrophobic paper can withstand a variety of wear and tear, such as tape stripping and knife scraping. The as-prepared superhydrophobic paper showed mechanical durability even after 20 wear cycles with sandpaper,thus sustaining excellent superhydrophobicity on the filter paper surface. Moreover, it remains fully functional even in environments with high concentrations of acid and alkali for 96 h. The superhydrophobicity was not affected after storage for 6 months in a natural environment. It was confirmed that the superhydrophobic paper surface exhibited excellent chemical stability, long-term stability and self-cleaning properties. The entire production process was operated under normal environment, without complex equipment; and, as such, has the possibility for large-scale production and application in industry.
- Published
- 2020
18. GENDER INEQUALITY, PAY PARITY AND PERFORMANCE. SYSTEMATIC REVIEW PAPER ON GENDER INEQUALITY, PERCEPTION OF PAY PARITY AND PERFORMANCE
- Author
-
Megha Jikar and Prateek Kanchan
- Subjects
Embryology ,Cell Biology ,Anatomy ,Developmental Biology - Abstract
This review paper focuses on the gender disparity and causes of gender inequality and sheds light on the important studies on the topic. It also sheds light on the pay parity and performance literature. This review paper also sheds light on the definition, conceptualization, and measurement of employee performance. This review paper has also shown a number of studies that linked the relationship between pay fairness, organizational justice and employee performance. This review paper summarizes these findings and suggests areas where more inquiry is needed to resolve conflicting results. Around more than 50 papers have been studies out of which 12 studies and few blogs and articles are taken into consideration which provides enough evidence to justify that Gender Inequality, Pay Parity could affect Corporate Performance.
- Published
- 2022
19. Fluorescent Immunological Paper-based Assay for Exosome detection
- Author
-
Surasak Kasetsirikul, Muhammad J. A. Shiddiky, and Nam-Trung Nguyen
- Subjects
Chemistry ,Paper based ,Fluorescence ,Exosome ,Cell biology - Abstract
This paper reports the development of fluorescent-linked immunosorbent paper-based assay for exosome detection. The paper-based device was fabricated with sandwich lamination for easy handling and was coated with exosome-specific antibody as a biosensing platform to detect exosome sample from the cell culture media. This assay employed fluorescent detection which is followed by tagging fluorophore-conjugated detecting antibody on exosome samples. The fluorescent readout was evaluated and quantified from image processing software. This assay can detect high concentration of exosome samples (~ 1010 exosome/mL). However, this assay has encountered various challenges. First, the exosome concentration prepared from cell culture media from cancer-derived ovarian and mesothelial cell lines may be insufficient to reach detectable range. Second, chemical contamination from exosome isolation kits may affect assay sensitivity. Therefore, assay optimization and minimizing chemical contamination are required which could enhance assay specificity and sensitivity.
- Published
- 2021
20. Engagement in PC-based, smartphone-based, and paper-based materials: Learning vocabulary through Chinese Stories
- Author
-
Yijen Wang
- Subjects
Embryology ,Vocabulary ,Multimedia ,Computer science ,media_common.quotation_subject ,Cell Biology ,Paper based ,Anatomy ,computer.software_genre ,computer ,Developmental Biology ,media_common - Published
- 2020
21. Urinary Extracellular Vesicles: A Position Paper by the Urine Task Force of the International Society for Extracellular Vesicles
- Author
-
Juan M. Falcón-Pérez, Dylan Burger, Aled Clayton, Lei Zheng, Uta Erdbrügger, Kerstin Junker, Luca Musante, Ewout J. Hoorn, I.V. Bijnsdorp, Kenneth W. Witwer, Harry Holthöfer, James W. Dear, Erik H. Koritzinsky, Benedetta Bussolati, Jason P. Webber, Elena S. Martens-Uzunova, Charles J. Blijdorp, Inge Mertens, Alicia Llorente, James M. Luther, Peter S.T. Yuen, Catherine Sánchez, Janne Leivo, Andrew F. Hill, Guido Jenster, Eline Oeyen, Visith Thongboonkerd, Cristina Grange, Metka Lenassi, Maija Puhka, Carolina Soekmadji, Volkert van Steijn, Francesc E. Borràs, James Brian Byrd, Martin E. van Royen, Gerald W. Verhaegh, Mark A. Knepper, John Klein, Connie R. Jimenez, Institute for Molecular Medicine Finland, University of Helsinki, Internal Medicine, Urology, Pathology, CCA - Cancer biology and immunology, CCA - Imaging and biomarkers, Medical oncology laboratory, and Amsterdam Neuroscience - Neurodegeneration
- Subjects
0301 basic medicine ,CLINICAL-APPLICATIONS ,Urine ,0302 clinical medicine ,Medicine ,Biomarker discovery ,Urinary Tract ,bladder ,biobank ,biomarkers ,extracellular vesicles ,kidney ,liquid biopsy ,prostate ,rigor and standardization ,urine ,DEEP SEQUENCING ANALYSIS ,Prostate ,TAMM-HORSFALL PROTEIN ,Reference Standards ,Extracellular vesicles ,BIOMARKER DISCOVERY ,Body Fluids ,3. Good health ,PROSTATE-CANCER ,Clinical Practice ,030220 oncology & carcinogenesis ,Position Paper ,PROTEOMIC ANALYSIS ,MESSENGER-RNA ,MEMBRANE-VESICLES ,Histology ,Urinary system ,Advisory Committees ,Scientific field ,Bladders ,DIABETIC-NEPHROPATHY ,03 medical and health sciences ,All institutes and research themes of the Radboud University Medical Center ,Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15] ,Humans ,Biology ,QH573-671 ,Task force ,business.industry ,Reproducibility of Results ,Kidneys ,Cell Biology ,Biobanks ,SODIUM-CHLORIDE COTRANSPORTER ,030104 developmental biology ,Clinical diagnosis ,Position paper ,1182 Biochemistry, cell and molecular biology ,Human medicine ,Societies ,business ,Cytology ,Position Papers ,Neuroscience ,Biomarkers - Abstract
Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field. ESM-U, CG, GV, GJ, IVB, MvR, and VvS, are members of the “IMMPROVE” consortium (Innovative Measurements and Markers for Prostate Cancer Diagnosis and Prognosis using Extracellular Vesicles), which is sponsored by an Alpe d'HuZes grant of the Dutch Cancer Society (grant #EMCR2015-8022). AL is supported by Norges Forskningsråd, Kreftforeningen and Helse Sør-Øst RHF (NO). UE is supported by the NIH, National Heart, Lung, and Blood Institute, Award number K23-HL-126101. CJB and EJH are supported by the Dutch Kidney Foundation (Nierstichting), Award number: CP18.05.
- Published
- 2021
22. Call for Papers: 'Novel Insights into Preterm Respiratory Physiology: Celebrating the 100th Birthday of Dr. Mildred T. Stahlman'
- Author
-
Rory Morty and Sue Bodine
- Subjects
Pulmonary and Respiratory Medicine ,Physiology ,Physiology (medical) ,Cell Biology - Published
- 2022
23. Considerations for immune effector cell therapy collections: a white paper from the American Society for Apheresis
- Author
-
Hien D. Liu, Leon Su, Jeffrey L. Winters, Suzanne R. Thibodeaux, Yara A. Park, YanYun Wu, Joseph Schwartz, Abba C. Zubair, Jennifer Schneiderman, Gaurav K. Gupta, Sharanya Ramakrishnan, and Nicole A. Aqui
- Subjects
Adult ,Cancer Research ,Transplantation ,Consensus ,Immunology ,Cell- and Tissue-Based Therapy ,Cell Biology ,Tissue Donors ,United States ,Oncology ,Blood Component Removal ,Humans ,Immunology and Allergy ,Leukapheresis ,Child ,Genetics (clinical) - Abstract
This white paper was developed to provide leukapheresis guidance for the collection of mononuclear cells from adult and pediatric patients who are destined for immune effector cell (IEC) therapies for commercial and research applications. Currently, there is considerable variability in leukapheresis processes and limited published information regarding best practices relevant to new cellular therapies, especially IECs. Herein the authors address critical leukapheresis questions in five domains to help guide consistent collection processes and ensure high-quality products. The first four domains are onboarding, pre-collection, collection and post-collection, with protocol feasibility, preparation, care and follow-up of the patient/donor at each step, respectively, and technical considerations during collection. The fifth domain of quality assurance focuses on ensuring product potency, purity, safety and auditing.The American Society for Apheresis (ASFA) Clinical Applications Committee (IEC Therapy Subcommittee) was charged by the society's board of directors with working collaboratively with other ASFA committees and organizations, including the Foundation for the Accreditation of Cellular Therapy, Association for the Advancement of Blood and Biotherapies, American Society for Transplantation and Cellular Therapy, National Marrow Donor Program and International Society for CellGene Therapy, to develop guidelines regarding leukapheresis collection of cells destined for the manufacture of IEC therapies. After a review of the literature and discussion with members of the involved committees and various institutions, a draft guidance was created and circulated for comment and revision.Critical aspects of apheresis that could affect the quality and quantity of the leukapheresis product were identified. These areas were then discussed and reviewed. After consensus, the best practice guidelines were proposed and accepted.In the current era of rapid growth of IEC therapies, it is important to address critical leukapheresis steps to provide high-quality products and more consistent practices and to eliminate redundant efforts.
- Published
- 2022
24. Paper-based ELISA diagnosis technology for human brucellosis based on a multiepitope fusion protein
- Author
-
Han Li, Hai Jiang, Mingjun Sun, Qiongqiong Bai, Dehui Yin, Jingpeng Zhang, Xiling Wu, and Jihong Shao
- Subjects
Bacterial Diseases ,Serum Proteins ,B Cells ,RC955-962 ,Disease ,Pathology and Laboratory Medicine ,Biochemistry ,Epitope ,Cell Fusion ,Epitopes ,White Blood Cells ,Medical Conditions ,Filter Paper ,Animal Cells ,Zoonoses ,Arctic medicine. Tropical medicine ,Medicine and Health Sciences ,Enzyme-Linked Immunoassays ,biology ,Bacterial Pathogens ,Laboratory Equipment ,medicine.anatomical_structure ,Infectious Diseases ,Medical Microbiology ,Engineering and Technology ,Pathogens ,Cellular Types ,Public aspects of medicine ,RA1-1270 ,Bacterial Outer Membrane Proteins ,Research Article ,Neglected Tropical Diseases ,China ,Cell Physiology ,Immune Cells ,Immunology ,Equipment ,Enzyme-Linked Immunosorbent Assay ,Brucella ,Research and Analysis Methods ,Sensitivity and Specificity ,Microbiology ,Brucellosis ,Antigen ,Diagnostic Medicine ,medicine ,Humans ,Immunoassays ,Antibody-Producing Cells ,Microbial Pathogens ,B cell ,Antigens, Bacterial ,Blood Cells ,Bacteria ,business.industry ,Public Health, Environmental and Occupational Health ,Organisms ,Biology and Life Sciences ,Proteins ,Cell Biology ,medicine.disease ,biology.organism_classification ,Tropical Diseases ,Fusion protein ,Virology ,Infectious disease (medical specialty) ,Immunologic Techniques ,business - Abstract
Background Brucellosis, as a serious zoonotic infectious disease, has been recognized as a re-emerging disease in the developing countries worldwide. In china, the incidence of brucellosis is increasing each year, seriously threatening the health of humans as well as animal populations. Despite a quite number of diagnostic methods currently being used for brucellosis, innovative technologies are still needed for its rapid and accurate diagnosis, especially in area where traditional diagnostic is unavailable. Methodology/Principal findings In this study, a total of 22 B cell linear epitopes were predicted from five Brucella outer membrane proteins (OMPs) using an immunoinformatic approach. These epitopes were then chemically synthesized, and with the method of indirect ELISA (iELISA), each of them displayed a certain degree of capability in identifying human brucellosis positive sera. Subsequently, a fusion protein consisting of the 22 predicted epitopes was prokaryotically expressed and used as diagnostic antigen in a newly established brucellosis testing method, nano-ZnO modified paper-based ELISA (nano-p-ELISA). According to the verifying test using a collection of sera collected from brucellosis and non-brucellosis patients, the sensitivity and specificity of multiepitope based nano-p-ELISA were 92.38% and 98.35% respectively. The positive predictive value was 98.26% and the negative predictive value was 91.67%. The multiepitope based fusion protein also displayed significantly higher specificity than Brucella lipopolysaccharide (LPS) antigen. Conclusions B cell epitopes are important candidates for serologically testing brucellosis. Multiepitope fusion protein based nano-p-ELISA displayed significantly sensitivity and specificity compared to Brucella LPS antigen. The strategy applied in this study will be helpful to develop rapid and accurate diagnostic method for brucellosis in human as well as animal populations., Author summary Brucellosis is one of the most important zoonosis in the world and has caused tremendous economic losses in agriculture and animal husbandry in many countries. Developing rapid, sensitive and specific diagnostic methods is very important for early detection and treatment of brucellosis patients. In this study, a novel diagnostic technique, nano-ZnO modified paper ELISA, was established. The antigen used in this technique was a fusion protein containing multiple B cell epitopes, which were predicted from Brucella major outer membrane proteins such as Bp26, Omp31, Omp16, Omp2b and Omp25. Comparing to traditional LPS antigen, this multiepitope based antigen displayed considerably higher sensitivity and higher specificity in laboratory. With the strategy described in this paper, more efficient epitopes and protein antigen can be identified in the future. Currently, LPS antigen is only prepared from live Brucella, while protein antigen can be produced in large quantities in prokaryotic expression system. In addition to nano-p-ELISA, this protein antigen can also be used for development other methods such as fluorescent polarization assay (FPA) and immunochromatographic assay (ICA) to meet the varied demand for brucellosis testing.
- Published
- 2021
25. A paper-based ELISA for rapid sensitive determination of anaphylaxis-related MRGPRX2 in human peripheral blood
- Author
-
Tao Zhang, Xiaoqian Li, Yuanyuan Ding, Shengli Han, Langchong He, Xinyan Dong, Liyun Kong, and Qingpeng Gao
- Subjects
Paper ,Receptors, Neuropeptide ,biology ,Chemistry ,Capture antibody ,Biophysics ,Enzyme-Linked Immunosorbent Assay ,Nerve Tissue Proteins ,Cell Biology ,Paper based ,medicine.disease ,Biochemistry ,Molecular biology ,Horseradish peroxidase ,Peripheral blood ,Receptors, G-Protein-Coupled ,Polyclonal antibodies ,biology.protein ,medicine ,Humans ,Bovine serum albumin ,Antibody ,Molecular Biology ,Anaphylaxis - Abstract
Mas-related G-protein-coupled receptor X2 (MRGPRX2) has recently been reported to be associated with anaphylaxis. Detection of MRGPRX2 levels in human peripheral blood might serve as a powerful tool for predicting the predisposition of patients to anaphylactic reactions. For rapid measurement of MRGPRX2, we established a paper-based double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) using mouse monoclonal antibody and horseradish peroxidase (HRP)-labelled rabbit polyclonal antibody as capture antibody and detection antibody, respectively. We avoided chemical functionalization of the cellulose paper by introducing bovine serum albumin (BSA) to provide COOH and NH2 groups for covalent immobilization of the capture antibody. Through amide condensation, a two-layer immobilization strategy was applied with BSA-BSA and BSA-capture antibody networks as the first and second layers, respectively. This strategy improved the quantity, activity and stability of the immobilized antibody. We then established a paper-based ELISA to detect MRGPRX2 in human peripheral blood. Our method is less laborious, easier to implement, and more cost-effective than conventional ELISA, while offering similar sensitivity, specificity, and accuracy. Therefore, it could serve as an innovative clinical point-of-care diagnostic tool, especially in areas that lack advanced clinical equipment.
- Published
- 2021
26. Survival of SARS-COV-2 under liquid medium, dry filter paper and acidic conditions
- Author
-
Zhiping Sun, Xia Cai, Wendong Han, Yang Wu, Chenjian Gu, Rong Zhang, Youhua Xie, Zhenghong Yuan, Di Qu, Wei Xu, Xunjia Cheng, Yuyan Wang, and Yun Qian
- Subjects
2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,Filter paper ,business.industry ,lcsh:Cytology ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Cell Biology ,Liquid medium ,Biochemistry ,Virology ,Correspondence ,Genetics ,Medicine ,lcsh:QH573-671 ,business ,Molecular Biology - Published
- 2020
27. Bibliometric analysis of scientific papers on extracellular vesicles in kidney disease published between 1999 and 2022
- Author
-
Marady Hun, Huai Wen, Phanna Han, Tharith Vun, Mingyi Zhao, and Qingnan He
- Subjects
Cell Biology ,Developmental Biology - Abstract
Background: In recent years, there has been an increasing interest in using extracellular vesicles (EVs) as potential therapeutic agents or natural drug delivery systems in kidney-related diseases. However, a detailed and targeted report on the current condition of extracellular vesicle research in kidney-related diseases is lacking. Therefore, this prospective study was designed to investigate the use of bibliometric analysis to comprehensively overview the current state of research and frontier trends on extracellular vesicle research in kidney-related diseases using visualization tools.Methods: The Web of Science Core Collection (WoSCC) database was searched to identify publications related to extracellular vesicle research in kidney-related diseases since 1999. Citespace, Microsoft Excel 2019, VOSviewer software, the R Bibliometrix Package, and an online platform were used to analyze related research trends to stratify the publication data and collaborations.Results: From 1 January 1999 to 26 June 2022, a total of 1,122 EV-related articles and reviews were published, and 6,486 authors from 1,432 institutions in 63 countries or regions investigated the role of extracellular vesicles in kidney-related diseases. We found that the number of articles on extracellular vesicles in kidney-related diseases increased every year. Dozens of publications were from China and the United States. China had the most number of related publications, in which the Southeast University (China) was the most active institution in all EV-related fields. Liu Bi-cheng published the most papers on extracellular vesicles, while Clotilde Théry had the most number of co-citations. Most papers were published by The International Journal of Molecular Sciences, while Kidney International was the most co-cited journal for extracellular vesicles. We found that exosome-related keywords included exosome, exosm, expression, extracellular vesicle, microRNA, microvesicle, and liquid biopsy, while disease- and pathological-related keywords included biomarker, microRNA, apoptosis, mechanism, systemic lupus erythematosus, EGFR, acute kidney injury, and chronic kidney disease. Acute kidney disease (AKI), CKD, SLE, exosome, liquid biopsy, and extracellular vesicle were the hotspot in extracellular vesicle and kidney-related diseases research.Conclusion: The field of extracellular vesicles in kidney-related disease research is rapidly growing, and its domain is likely to expand in the next decade. The findings from this comprehensive analysis of extracellular vesicles in kidney-related disease research could help investigators to set new diagnostic, therapeutic, and prognostic ideas or methods in kidney-related diseases.
- Published
- 2023
28. Paper-based Transwell assays: an inexpensive alternative to study cellular invasion
- Author
-
Adam Loeser, Nathan A. Whitman, Matthew R. Lockett, and Rachael M. Kenney
- Subjects
Paper ,02 engineering and technology ,01 natural sciences ,Biochemistry ,Article ,Analytical Chemistry ,Extracellular matrix ,Cell Movement ,Cell Line, Tumor ,Electrochemistry ,Animals ,Humans ,Environmental Chemistry ,Neoplasm Invasiveness ,Spectroscopy ,Chemistry ,010401 analytical chemistry ,Exogenous factor ,Disease progression ,Reproducibility of Results ,Cell movement ,Paper based ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Cell biology ,Cell culture ,Biological Assay ,Cattle ,0210 nano-technology - Abstract
Cellular movement is essential in the formation and maintenance of healthy tissues as well as in disease progression such as tumor metastasis. In this work, we describe a paper-based Transwell assay capable of quantifying cellular invasion through an extracellular matrix. The paper-based Transwell assays generate similar datasets, with equivalent reproducibility, to commercially available Transwell assays. With different culture configurations, we quantify invasion: upon addition of an exogenous factor or in the presence of medium obtained from other cell types, in an indirect or direct co-culture format whose medium composition is dynamically changing, and in a single-zone or parallel (96-zone) format.
- Published
- 2019
29. Paper-based plasmonic substrates as surface-enhanced Raman scattering spectroscopy platforms for cell culture applications
- Author
-
L. Guerrini, Yanan Kang, K. Juarez-Moreno, José M. Romo-Herrera, R.A. Alvarez-Puebla, Wolfgang J. Parak, N. Feliu, and Publica
- Subjects
Medicine (General) ,Materials science ,Biocompatibility ,QH301-705.5 ,Biomedical Engineering ,Nanoparticle ,Bioengineering ,Nanotechnology ,Review Article ,Nanomaterials ,Biomaterials ,R5-920 ,Biology (General) ,Plasmonic nanoparticles ,Molecular Biology ,Plasmon ,SERS ,Paper-based substrates ,Cell Biology ,Plasmonic papers ,Colloidal gold ,Cell culture ,Biosensor ,Biotechnology - Abstract
The engineering of advanced materials capable of mimicking the cellular micro-environment while providing cells with physicochemical cues is central for cell culture applications. In this regard, paper meets key requirements in terms of biocompatibility, hydrophilicity, porosity, mechanical strength, ease of physicochemical modifications, cost, and ease of large-scale production, to be used as a scaffold material for biomedical applications. Most notably, paper has demonstrated the potential to become an attractive alternative to conventional biomaterials for creating two-dimensional (2D) and three-dimensional (3D) biomimetic cell culture models that mimic the features of in vivo tissue environments for improving our understanding of cell behavior (e.g. growth, cell migration, proliferation, differentiation and tumor metastasis) in their natural state. On the other hand, integration of plasmonic nanomaterials (e.g. gold nanoparticles) within the fibrous structure of paper opens the possibility to generate multifunctional scaffolds equipped with biosensing tools for monitoring different cell cues through physicochemical signals. Among different plasmonic based detection techniques, surface-enhanced Raman scattering (SERS) spectroscopy emerged as a highly specific and sensitive optical tool for its extraordinary sensitivity and the ability for multidimensional and accurate molecular identification. Thus, paper-based plasmonic substrates in combination with SERS optical detection represent a powerful future platform for monitoring cell cues during cell culture processes. To this end, in this review, we will describe the different methods for fabricating hybrid paper-plasmonic nanoparticle substrates and their use in combination with SERS spectroscopy for biosensing and, more specifically, in cell culture applications., Graphical abstract Image 1
- Published
- 2021
30. BBRC for 2023 and beyond - Research papers, reviews and the Carafoli Medal
- Author
-
Wolfgang Baumeister and Joe d’Angelo
- Subjects
Biophysics ,Cell Biology ,Molecular Biology ,Biochemistry - Published
- 2023
31. Call for papers: cyclical function of the female reproductive tract
- Author
-
Michele Boiani and Francesca E Duncan
- Subjects
Embryology ,Reproductive Medicine ,Genetics ,Obstetrics and Gynecology ,Cell Biology ,Molecular Biology ,Developmental Biology - Published
- 2023
32. Growing a circular economy with fungal biotechnology: a white paper
- Author
-
Meyer, Vera, Basenko, Evelina Y, Benz, J Philipp, Braus, Gerhard H, Caddick, Mark X, Csukai, Michael, de Vries, Ronald P, Endy, Drew, Frisvad, Jens C, Gunde-Cimerman, Nina, Haarmann, Thomas, Hadar, Yitzhak, Hansen, Kim, Johnson, Robert I, Keller, Nancy P, Kraševec, Nada, Mortensen, Uffe H, Perez, Rolando, Ram, Arthur F J, Record, Eric, Ross, Phil, Shapaval, Volha, Steiniger, Charlotte, van den Brink, Hans, van Munster, Jolanda, Yarden, Oded, Wösten, Han A B, Sub Molecular Plant Physiology, Sub Molecular Microbiology, Molecular Microbiology, Molecular Plant Physiology, Department of Molecular Microbiology and Biotechnology, Leiden University, Institute of Biology Leiden, Georg-August-University [Göttingen], School of Biological Sciences, University of Liverpool, Science Faculty, Mohamed V University in Rabat, Chercheur indépendant, Technical University of Denmark [Lyngby] (DTU), Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins, University of Virginia [Charlottesville], Centre of Molecular Immunology, Antibody engineering Department, Leiden University, Unité de microbiologie et technologie céréalières, Institut National de la Recherche Agronomique (INRA), Norwegian University of Life Sciences (NMBU), Department of Plant Pathology and Microbiology, The Hebrew University of Jerusalem (HUJ), Utrecht University [Utrecht], Sub Molecular Plant Physiology, Sub Molecular Microbiology, Molecular Microbiology, Molecular Plant Physiology, Westerdijk Fungal Biodiversity Institute - Fungal Physiology, and Westerdijk Fungal Biodiversity Institute
- Subjects
0106 biological sciences ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,lcsh:Biotechnology ,[SDV]Life Sciences [q-bio] ,Population ,Automotive industry ,Review ,01 natural sciences ,Applied Microbiology and Biotechnology ,12. Responsible consumption ,03 medical and health sciences ,White paper ,lcsh:TP248.13-248.65 ,010608 biotechnology ,11. Sustainability ,SDG 13 - Climate Action ,education ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,2. Zero hunger ,Sustainable development ,0303 health sciences ,education.field_of_study ,business.industry ,Circular economy ,SDG 8 - Decent Work and Economic Growth ,Cell Biology ,[SDE.ES]Environmental Sciences/Environmental and Society ,Biotechnology ,Climate change mitigation ,13. Climate action ,Greenhouse gas ,Sustainability ,Business ,SDG 12 - Responsible Consumption and Production - Abstract
Fungi have the ability to transform organic materials into a rich and diverse set of useful products and provide distinct opportunities for tackling the urgent challenges before all humans. Fungal biotechnology can advance the transition from our petroleum-based economy into a bio-based circular economy and has the ability to sustainably produce resilient sources of food, feed, chemicals, fuels, textiles, and materials for construction, automotive and transportation industries, for furniture and beyond. Fungal biotechnology offers solutions for securing, stabilizing and enhancing the food supply for a growing human population, while simultaneously lowering greenhouse gas emissions. Fungal biotechnology has, thus, the potential to make a significant contribution to climate change mitigation and meeting the United Nation’s sustainable development goals through the rational improvement of new and established fungal cell factories. The White Paper presented here is the result of the 2nd Think Tank meeting held by the EUROFUNG consortium in Berlin in October 2019. This paper highlights discussions on current opportunities and research challenges in fungal biotechnology and aims to inform scientists, educators, the general public, industrial stakeholders and policymakers about the current fungal biotech revolution.
- Published
- 2020
33. ESC Working Group on Cellular Biology of the Heart: position paper for Cardiovascular Research: tissue engineering strategies combined with cell therapies for cardiac repair in ischaemic heart disease and heart failure
- Author
-
Fabrice Prunier, Péter Ferdinandy, Joost P.G. Sluijter, Sandrine Lecour, Sophie Van Linthout, Linda W. van Laake, Maurizio Pesce, Hans Erik Bøtker, Kirsti Ytrehus, Sean M. Davidson, Felix B. Engel, Rosalinda Madonna, Francesco Fernandez-Aviles, Raffaele De Caterina, Cinzia Perrino, Philippe Menasché, Derek J. Hausenloy, Thomas Eschenhagen, Wolfram-Hubertus Zimmermann, Jonathan Leor, Jean-Sébastien Hulot, and Perrino, C
- Subjects
0301 basic medicine ,Biomedical Research ,Consensus ,Physiology ,Cells ,Cell ,Cardiology ,Myocardial Ischemia ,Cardiac tissue engineering ,030204 cardiovascular system & hematology ,Biomaterials ,Cell therapy ,03 medical and health sciences ,0302 clinical medicine ,Tissue engineering ,Physiology (medical) ,medicine ,Humans ,Regeneration ,Survival rate ,Heart Failure ,Heart failure ,Ischaemic heart disease ,Tissue Engineering ,business.industry ,Myocardium ,Regeneration (biology) ,Position Paper from European Society of Cardiology Working Group ,Recovery of Function ,medicine.disease ,Cell biology ,Transplantation ,Treatment Outcome ,030104 developmental biology ,medicine.anatomical_structure ,Position paper ,Cardiology and Cardiovascular Medicine ,business ,Stem Cell Transplantation - Abstract
Morbidity and mortality from ischaemic heart disease (IHD) and heart failure (HF) remain significant in Europe and are increasing worldwide. Patients with IHD or HF might benefit from novel therapeutic strategies, such as cell-based therapies. We recently discussed the therapeutic potential of cell-based therapies and provided recommendations on how to improve the therapeutic translation of these novel strategies for effective cardiac regeneration and repair. Despite major advances in optimizing these strategies with respect to cell source and delivery method, the clinical outcome of cell-based therapy remains unsatisfactory. Major obstacles are the low engraftment and survival rate of transplanted cells in the harmful microenvironment of the host tissue, and the paucity or even lack of endogenous cells with repair capacity. Therefore, new ways of delivering cells and their derivatives are required in order to empower cell-based cardiac repair and regeneration in patients with IHD or HF. Strategies using tissue engineering (TE) combine cells with matrix materials to enhance cell retention or cell delivery in the transplanted area, and have recently received much attention for this purpose. Here, we summarize knowledge on novel approaches emerging from the TE scenario. In particular, we will discuss how combinations of cell/bio-materials (e.g. hydrogels, cell sheets, prefabricated matrices, microspheres, and injectable matrices) combinations might enhance cell retention or cell delivery in the transplantation areas, thereby increase the success rate of cell therapies for IHD and HF. We will not focus on the use of classical engineering approaches, employing fully synthetic materials, because of their unsatisfactory material properties which render them not clinically applicable. The overall aim of this Position Paper from the ESC Working Group Cellular Biology of the Heart is to provide recommendations on how to proceed in research with these novel TE strategies combined with cell-based therapies to boost cardiac repair in the clinical settings of IHD and HF. © Published on behalf of the European Society of Cardiology. All rights reserved.
- Published
- 2019
34. LSA-50 paper: An alternative to P81 phosphocellulose paper for radiometric protein kinase assays
- Author
-
Rudra Kashyap, Johan Van Lint, Olivia Appelmans, Arnout Voet, Wim M. De Borggraeve, and Philippe Gilles
- Subjects
Paper ,Biochemistry & Molecular Biology ,Cation exchange paper ,Biophysics ,Biochemistry ,Biochemical Research Methods ,Protein kinase ,Research community ,Alternative paper ,Luciferase ,Kinase activity ,Cellulose ,Radiometry ,Protein kinase A ,Molecular Biology ,Science & Technology ,Kinase ,Chemistry ,Chemistry, Analytical ,Cell Biology ,Radiometric kinase assay ,Physical Sciences ,Phosphocellulose paper ,Life Sciences & Biomedicine ,Protein Kinases - Abstract
Radiometric assays have widely been used for measuring protein kinase activity for decades. In addition, several non-radiometric kinase assay formats have been developed over the years, including luciferase-based and fluorescence-based assays. However, radiometric assays are still considered as the "gold standard" for protein kinase assays, because of their direct readout, high sensitivity, reproducibility, reliability, and very low background signals. These radiometric assays rely on P81 phosphocellulose paper to capture the phosphorylated substrate and wash out unreacted [γ-32P] ATP. However, recently the production of P81 was discontinued by the manufacturer, causing major concern within the protein kinase research community. The advantages of radiometric assays over other kinase assay methods call for an urgent alternative to the discontinued P81 paper. In this report, we demonstrate that the LSA-50 paper is a worthy alternative for radiometric protein kinase assays originally using P81 phosphocellulose paper. ispartof: ANALYTICAL BIOCHEMISTRY vol:630 ispartof: location:United States status: published
- Published
- 2021
35. A RESEARCH PAPER ON STRATEGIES THAT BUSINESSES USE TO SURVIVE THROUGH SOCIAL MEDIA MARKETING WITH REFERENCE TO INSTAGRAM
- Author
-
Pulkit Trivedi and Ruma Pal
- Subjects
Embryology ,Cell Biology ,Anatomy ,Developmental Biology - Abstract
Companies are turning to social media marketing as the newest means of promoting their products and services. Instagram, a photo-sharing app for mobile devices, has emerged as a critical marketing tool. This research aims to determine which of the three Instagram marketing strategies is the most effective in terms of growing a following, increasing brand recognition, and boosting sales. Many secondary sources were also used to support the primary research. Instagram users in the city of Ahmedabad were surveyed to gather information. T-tests, Chi-Square, and descriptive statistics were used to examine the data further. A recent study found that Instagram users take a variety of things into account when deciding whether or not to follow a brand or post about a product or service. According to our survey, the vast majority of those who responded were utilizing Instagram to promote their brands and increase sales on their e-commerce sites. Generally, Instagram users create traffic by arranging their grids attractively, publishing consistently, following Instagram trends, and offering discounts and coupons. Marketers may use Instagram to their advantage by creating unique and engaging content. Because of Instagram's emphasis on visual features such as photographs and videos, marketers now have a new channel through which to reach customers. Customers and businesses may converse more easily and casually on Instagram because of the platform's more relaxed atmosphere.
- Published
- 2022
36. MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines
- Author
-
Xianlin Han, Tze Ping Loh, Bo Burla, Michelle K. Lin, Makoto Arita, Masanori Arita, Edward A. Dennis, Kim Ekroos, Anne K. Bendt, Michael J.O. Wakelam, Amaury Cazenave-Gassiot, Federico Torta, Oswald Quehenberger, Markus R. Wenk, Kazutaka Ikeda, Craig E. Wheelock, Andrej Shevchenko, Matej Orešič, Peter J. Meikle, and Gerhard Liebisch
- Subjects
0301 basic medicine ,Sample handling ,Human blood ,business.industry ,ta1182 ,Cell Biology ,Computational biology ,Shotgun lipidomics ,Lipidome ,Biochemistry ,Biological fluid ,3. Good health ,03 medical and health sciences ,030104 developmental biology ,Endocrinology ,Lipidomics ,Blood plasma ,Position paper ,Medicine ,business - Abstract
Human blood is a self-regenerating lipid-rich biological fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma (plasma lipidome) offers insights into individual metabolism and physiology in health and disease. Disturbances in the plasma lipidome also occur in conditions that are not directly linked to lipid metabolism; therefore, plasma lipidomics based on MS is an emerging tool in an array of clinical diagnostics and disease management. However, challenges exist in the translation of such lipidomic data to clinical applications. These relate to the reproducibility, accuracy, and precision of lipid quantitation, study design, sample handling, and data sharing. This position paper emerged from a workshop that initiated a community-led process to elaborate and define a set of generally accepted guidelines for quantitative MS-based lipidomics of blood plasma or serum, with harmonization of data acquired on different instrumentation platforms across independent laboratories as an ultimate goal. We hope that other fields may benefit from and follow such a precedent.
- Published
- 2018
37. Who did what: changing how science papers are written to detail author contributions
- Author
-
Oded Rechavi and Pavel Tomancak
- Subjects
Cell Biology ,Molecular Biology - Published
- 2023
38. Dopamine-polyethyleneimine co-deposition cellulose filter paper for α-Glucosidase immobilization and enzyme inhibitor screening
- Author
-
Juan Chen, Xiao-Hui Ma, Peng Li, and Ling Jin
- Subjects
Paper ,Immobilized enzyme ,Dopamine ,Clinical Biochemistry ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Capillary electrophoresis ,medicine ,Polyethyleneimine ,Glycoside Hydrolase Inhibitors ,Cellulose ,Acarbose ,chemistry.chemical_classification ,Chromatography ,biology ,Filter paper ,Chemistry ,010401 analytical chemistry ,Temperature ,Electrophoresis, Capillary ,alpha-Glucosidases ,Cell Biology ,General Medicine ,Repeatability ,Hydrogen-Ion Concentration ,Enzymes, Immobilized ,0104 chemical sciences ,Enzyme ,Enzyme inhibitor ,biology.protein ,medicine.drug ,Drugs, Chinese Herbal - Abstract
In this work, cellulose filter paper (CFP), which is inexpensive and commercially available, was used as the carrier, and the immobilized α-glucosidase was obtained by two steps: firstly, the surface of CFP was modified by polydopamine/polyethyleneimine (PDA/PEI) co-deposition method to obtain CFP-PDA/PEI with a uniform coating of rich positive charge; subsequently, α-glucosidase was immobilized on the CFP-PDA/PEI by electrostatic adsorption. The free enzyme and immobilized enzyme have the same optimal temperature (70℃) and pH (8.0), and their Km is similar, which is 2.2 and 2.8, respectively. These results show that the immobilization process does not change the properties of the enzyme greatly. The immobilized enzyme still maintains 75.6% of its initial activity after 10 repeated uses, showing good reusability. The excellent repeatability (RSD = 2.2%, n = 5) and the verification of competitive inhibitor (acarbose) illustrates the reliability of the immobilized enzymes for enzyme inhibitor screening. Finally, combined with CE, a screening method based immobilized α-glucosidase was proposed and applied to screen the α-glucosidase inhibitory from 10 kinds of Traditional Chinese medicines (TCMs) in vitro. The results indicated that the method was a very effective tool for screening potential α-glucosidase inhibitors from TCMs.
- Published
- 2020
39. A low-cost paper-based aptasensor for simultaneous trace-level monitoring of mercury (II) and silver (I) ions
- Author
-
Zahra Khoshbin, Asma Verdian, and Mohammad Reza Housaindokht
- Subjects
Paper ,Materials science ,Silver ,Metal ions in aqueous solution ,Aptamer ,Inorganic chemistry ,Biophysics ,chemistry.chemical_element ,Biosensing Techniques ,01 natural sciences ,Biochemistry ,Ion ,03 medical and health sciences ,Environmental systems ,Molecular Biology ,030304 developmental biology ,0303 health sciences ,010401 analytical chemistry ,Cell Biology ,Paper based ,Mercury ,Aptamers, Nucleotide ,Fluorescence ,0104 chemical sciences ,Mercury (element) ,chemistry ,Graphite - Abstract
Mercury (Hg2+) and silver (Ag+) ions possess the harmful effects on public health and environment that makes it essential to develop the sensing techniques with great sensitivity for the ions. Metal ions commonly coexist in the different biological and environmental systems. Hence, it is an urgent demand to design a simple method for the simultaneous detection of metal ions, peculiarly in the case of coexisting Hg2+ and Ag+. This study introduces a low-cost paper-based aptasensor to monitor Hg2+ and Ag+, simultaneously. The strategy of the sensing array is according to the conformational changes of Hg2+- and Ag+-specific aptamers and their release from the GO surface after the injection of the target sample on the sensing platform. Through monitoring the fluorescence recovery changes against the concentrations of the ions, Hg2+ and Ag+ can be determined as low as 1.33 and 1.01 pM. The paper-based aptasensor can simultaneously detect the ions within about 10 min. The aptasensor is applied prosperously to monitor Hg2+ and Ag+ in human serum, water, and milk. The designed aptasensor with the main advantages of simplicity and feasibility holds the supreme potential to develop a cost-effective sensing method for environmental monitoring, food control, and human diagnostics.
- Published
- 2020
40. Protein measurements in venous plasma, earlobe capillary plasma and in plasma stored on filter paper
- Author
-
Thomas Pekar, Benjamin Siart, Masood Kamali-Moghaddam, Felipe Marques Souza de Oliveira, Bernard Wallner, Johan Björkesten, Qiujin Shen, Ralf Steinborn, and Alfred Nimmerichter
- Subjects
Adult ,Male ,Analyte ,Extension assay ,Capillary action ,Biophysics ,Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) ,Biochemistry ,Specimen Handling ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Phlebotomy ,Venules ,Capillary Plasma ,medicine ,Humans ,Multiplex ,Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) ,Molecular Biology ,Earlobe ,030304 developmental biology ,0303 health sciences ,Chromatography ,Filter paper ,Chemistry ,Inflammation protein biomarkers ,Ear ,Venous Plasma ,Blood Proteins ,Cell Biology ,Venous blood ,Dried plasma spots ,Healthy Volunteers ,medicine.anatomical_structure ,Earlobe capillary ,Cytokines ,Biomarkers ,030217 neurology & neurosurgery - Abstract
In this study, levels of inflammatory protein biomarkers in venous plasma, plasma derived from capillary blood from the earlobe, and capillary plasma stored as dried plasma spots (DPS) were compared. Samples from 12 male individuals were assessed with a panel of 92 inflammation-related proteins using multiplex proximity extension assay. Correlations between sample types varied greatly between analytes. A high correlation of rho > 0.8 was observed between capillary plasma and DPS for 32 analytes. At this level of correlation, 13 analytes correlated between venous and capillary plasma and 5 analytes in the comparison of venous blood with DPS.
- Published
- 2019
41. Design and fabrication of microfibrous composite scaffold by coating clindamycin and chitosan onto cellulose filter paper for wound dressing applications
- Author
-
Sajjad Haider, Nadia Farooq, Rawaiz khan, Syed Babar Jamal, Dalal alotaibi, Bushra Bano, Nargis Jamila, Muhammad Naeem, Ali alrahlah, Muhammad Umar Aslam Khan, Adnan Haider, and Naeem Khan
- Subjects
Materials Science (miscellaneous) ,Cell Biology ,Electrical and Electronic Engineering ,Physical and Theoretical Chemistry ,Atomic and Molecular Physics, and Optics ,Biotechnology - Published
- 2022
42. Three overlooked photosynthesis papers of Otto Warburg (1883–1970), published in the 1940s in German and in Russian, on light-driven water oxidation coupled to benzoquinone reduction
- Author
-
Holger Dau, Govindjee Govindjee, Boris Ivanov, Dmitry Shevela, and William H. Armstrong
- Subjects
German ,Philosophy ,Light driven ,language ,Cell Biology ,Plant Science ,General Medicine ,Photosynthesis ,Biochemistry ,Benzoquinone ,Classics ,language.human_language - Abstract
After a brief background on Otto Heinrich Warburg (1883–1970), and some of his selected research, we provide highlights, in English, of three of his papers in the 1940s—unknown to many as they were not originally published in English. They are: two brief reports on Photosynthesis, with Wilhelm Luttgens, originally published in German, in 1944: ‘Experiment on assimilation of carbonic acid’; and ‘Further experiments on carbon dioxide assimilation’. This is followed by a regular paper, originally published in Russian, in 1946: ‘The photochemical reduction of quinone in green granules’. Since the 1944 reports discussed here are very short, their translations are included in the Appendix, but that of the 1946 paper is provided in the Supplementary Material. In all three reports, Warburg provides the first evidence for and elaborates on light-driven water oxidation coupled to reduction of added benzoquinone. These largely overlooked studies of Warburg are in stark contrast to Warburg’s well-known error in assigning the origin of the photosynthetically formed dioxygen to carbonate.
- Published
- 2021
43. Paper-based in vitro tissue chip for delivering programmed mechanical stimuli of local compression and shear flow
- Author
-
Marianne Madias, Patarajarin Akarapipad, Jeong Yeol Yoon, Kattika Kaarj, and Soohee Cho
- Subjects
0301 basic medicine ,Microcontroller ,Environmental Engineering ,Materials science ,Paper-based cell culture ,Biomedical Engineering ,02 engineering and technology ,Bending ,Servomotor ,03 medical and health sciences ,Automated flow control ,Tissue Chip ,Cell migration ,lcsh:QH301-705.5 ,Molecular Biology ,ComputingMethodologies_COMPUTERGRAPHICS ,Methodology ,Cell Biology ,Vascular endothelial cell ,021001 nanoscience & nanotechnology ,Compression (physics) ,Chip ,Shear (sheet metal) ,030104 developmental biology ,lcsh:Biology (General) ,0210 nano-technology ,Shear flow ,Biomedical engineering - Abstract
Abstract Mechanical stimuli play important roles on the growth, development, and behavior of tissue. A simple and novel paper-based in vitro tissue chip was developed that can deliver two types of mechanical stimuli—local compression and shear flow—in a programmed manner. Rat vascular endothelial cells (RVECs) were patterned on collagen-coated nitrocellulose paper to create a tissue chip. Localized compression and shear flow were introduced by simply tapping and bending the paper chip in a programmed manner, utilizing an inexpensive servo motor controlled by an Arduino microcontroller and powered by batteries. All electrical compartments and a paper-based tissue chip were enclosed in a single 3D-printed enclosure, allowing the whole device to be independently placed within an incubator. This simple device effectively simulated in vivo conditions and induced successful RVEC migration in as early as 5 h. The developed device provides an inexpensive and flexible alternative for delivering mechanical stimuli to other in vitro tissue models. Graphical abstract
- Published
- 2020
44. Heat-induced antigen retrieval utilizing modified Tris-EDTA buffer for reprobing of Western blots on nitrocellulose paper
- Author
-
Jun-Ling Wang, Ze-Ting Xu, Ling Zhan, Min Liao, and Chao-Jin Xu
- Subjects
Biophysics ,Cell Biology ,Molecular Biology ,Biochemistry - Published
- 2023
45. The leading role of pathology in assessing the somatic molecular alterations of cancer: Position Paper of the European Society of Pathology
- Author
-
Matias-Guiu, Xavier, Stanta, Giorgio, Carneiro, Fátima, Ryska, Ales, Hoefler, Gerald, Moch, Holger, European Society of Pathology (ESP), University of Zurich, and Matias-Guiu, Xavier
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Pre-analytical issues ,Somatic cell ,Medical laboratory ,610 Medicine & health ,Context (language use) ,Pathology and Forensic Medicine ,1307 Cell Biology ,03 medical and health sciences ,0302 clinical medicine ,10049 Institute of Pathology and Molecular Pathology ,Neoplasms ,1312 Molecular Biology ,medicine ,Biomarkers, Tumor ,Molecular diagnostics ,Humans ,Patologia molecular ,Pathology, Molecular ,Precision Medicine ,Càncer ,Molecular Biology ,Cancer ,Molecular pathology ,business.industry ,Cell Biology ,General Medicine ,medicine.disease ,Molecular analysis ,2734 Pathology and Forensic Medicine ,Review and Perspectives ,Europe ,Pathologists ,030104 developmental biology ,030220 oncology & carcinogenesis ,Scientific domain ,Commentary ,Position paper ,business ,Integrative pathology - Abstract
Molecular pathology is an essential part of pathology complementing conventional morphological tools to obtain a correct integrated diagnosis with appropriate assessment of prognosis and prediction of response to therapy, particularly in cancer. There is a concern about the situation of molecular pathology in some areas of Europe, namely, regarding the central role of pathologists in assessing somatic genomic alterations in cancer. In some countries, there are attempts that other laboratory medicine specialists perform the molecular analysis of somatic alterations in cancer, particularly now when next generation sequencing (NGS) is incorporated into clinical practice. In this scenario, pathologists may play just the role of "tissue providers," and other specialists may take the lead in molecular analysis. Geneticists and laboratory medicine specialists have all background and skills to perform genetic analysis of germline alterations in hereditary disorders, including familial forms of cancers. However, interpretation of somatic alterations of cancer belongs to the specific scientific domain of pathology. Pathologists are necessary to guarantee the quality of the results, for several reasons: (1) The identified molecular alterations should be interpreted in the appropriate morphologic context, since most of them are context-specific; (2) pre-analytical issues must be taken into consideration; (3) it is crucial to check the proportion of tumor cells in the sample subjected to analysis and presence of inflammatory infiltrate and necrosis should be monitored; and 4) the role of pathologists is crucial to select the most appropriate methods and to control the turnaround time in which the molecular results are delivered in the context of an integrated diagnosis. Obviously, there is the possibility of having core facilities for NGS in a hospital to perform the sequence analysis that are open to other specialties (microbiologists, geneticists), but also in this scenario, pathologists should have the lead in assessing somatic alterations of cancer. In this article, we emphasize the importance of interpreting somatic molecular alterations of the tumors in the context of morphology. In this Position Paper of the European Society of Pathology, we strongly support a central role of pathology departments in the process of analysis and interpretation of somatic molecular alterations in cancer. The proposal for this position paper was approved by the Publication Task Force of the European Society of Pathology (ESP), and this approval was subsequently verified by the Council of ESP. We thank Mar Varela, Dolors Cuevas, Ana Velasco and Jordi Temprana for help in writing the manuscript.
- Published
- 2019
46. Development of a low-cost paper-based ELISA method for rapid Escherichia coli O157:H7 detection
- Author
-
Kaiyue Fu, Xiuling Song, Kun Xu, Hao Bao, Dandan Song, Juan Li, Xiaofeng Qu, Xiangjun Meng, Li Li, Zhuping Zhang, Juan Wang, Chao Zhao, Bo Pang, and Yushen Liu
- Subjects
Paper ,Detection limit ,Chromatography ,Chemistry ,010401 analytical chemistry ,Biophysics ,Enzyme-Linked Immunosorbent Assay ,Pathogenic bacteria ,02 engineering and technology ,Cell Biology ,Paper based ,Escherichia coli O157 ,021001 nanoscience & nanotechnology ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,medicine ,Lower cost ,Elisa method ,0210 nano-technology ,Molecular Biology ,Escherichia coli - Abstract
Escherichia coli O157: H7 (E. coli O157: H7) has become one of the most dangerous foodborne pathogenic bacteria around the world. Currently, because of the tedious, high-cost and stringent laboratory conditions required, the conventional E. coli O157: H7 detection methods, such as culture-based methods and polymerase chain reaction (PCR), have much limitation. Thus, we developed a novel paper-based enzyme-linked immunosorbent assay (p-ELISA) with shorter operation duration, lower cost, relatively higher sensitivity and wider application. This method required less than 3 h and 5 μL of sample to complete the detection. The limit of detection (LOD) for E. coli O157: H7 reached 1 × 104 CFU/mL with high specificity. To be more suitable for on-site testing, the readout could be rapidly obtained without any expensive instruments. In this study, we chose E. coli O157:H7 as the representative, and our method could provide a platform for determination of other pathogenic bacteria.
- Published
- 2018
47. Nanos gigantium humeris insidentes: old papers informing new research into Toxoplasma gondii.
- Author
-
Lodoen, Melissa B., Smith, Nicholas C., Soldati-Favre, Dominique, Ferguson, David J.P., and van Dooren, Giel G.
- Subjects
- *
TOXOPLASMA gondii , *PARASITES , *PARASITE life cycles , *CYTOLOGY , *INTRACELLULAR pathogens , *TOXOPLASMA , *SCIENTIFIC community - Abstract
[Display omitted] • Seminal papers in Toxoplasma research are revisited. • We focus on a paper that contributed to uncovering the complete life-cycle of Toxoplasma. • A paper that revealed some of the unique cellular biology of the parasite is examined. • A study that elucidated the nature of the compartment in which Toxoplasma resides is described. • We revisit a seminal paper in understanding the immune responses to Toxoplasma infection. Since Nicolle, Manceaux and Splendore first described Toxoplasma gondii as a parasite of rodents and rabbits in the early 20th century, a diverse and vigorous research community has been built around studying this fascinating intracellular parasite. In addition to its importance as a pathogen of humans, livestock and wildlife, modern researchers are attracted to T. gondii as a facile experimental system to study many aspects of evolutionary biology, cellular biology, host-microbe interactions, and host immunity. For new researchers entering the field, the extensive literature describing the biology of the parasite, and the interactions with its host, can be daunting. In this review, we examine four foundational studies that describe various aspects of T. gondii biology, presenting a 'journal club'-style analysis of each. We have chosen a paper that established the beguiling life cycle of the parasite (Hutchison et al., 1971), a paper that described key features of its cellular biology that the parasite shares with related organisms (Gustafson et al., 1954), a paper that characterised the origin of the unique compartment in which the parasite resides within host cells (Jones and Hirsch, 1972), and a paper that established a key mechanism in the host immune response to parasite infection (Pfefferkorn, 1984). These interesting and far-reaching studies set the stage for subsequent research into numerous facets of parasite biology. As well as providing new researchers with an entry point into the literature surrounding the parasite, revisiting these studies can remind us of the roots of our discipline, how far we have come, and the new directions in which we might head. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
48. The raw truth about paper mills
- Author
-
Jana Christopher
- Subjects
Publishing ,0303 health sciences ,Biomedical Research ,Computer science ,030302 biochemistry & molecular biology ,Biophysics ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Cell Biology ,Biochemistry ,Data science ,03 medical and health sciences ,Structural Biology ,Genetics ,Humans ,Periodicals as Topic ,Raw data ,Molecular Biology ,Relevant information ,030304 developmental biology - Abstract
In 2018, the community first observed scientific papers in the biomedical literature that seemed to display systematically fabricated data, pointing to the existence of paper mills: unofficial, potentially illegal organizations selling fake scientific manuscripts. In the present article, we share relevant information specifically about the 'raw data' associated with paper mill manuscripts. If a submitted manuscript displays clear indicators of potential paper mill involvement, we found that the raw data at close inspection often raise doubts about their authenticity. In the absence of real data, paper mills may need to fabricate raw data images when responding to requests from journals. Given the necessity to streamline production of fake manuscripts, the alleged raw data might be created using templates. Some potential methods for generating fake Western blot images are discussed. Paying close attention to image data, including graphs, diagrams, plots and tables, ideally at pre-publication stage, can clearly help prevent publication of incorrect and fabricated information.
- Published
- 2021
49. Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
- Author
-
Dohwan Lee, Jeong Hoon Lee, Hyerin Kim, Kyung Wook Wee, Sung Il Han, Seungmin Kwak, and Dae Sung Yoon
- Subjects
chemistry.chemical_classification ,Paper ,General Immunology and Microbiology ,Biomolecule ,Microvesicle ,Vesicle ,General Chemical Engineering ,General Neuroscience ,010401 analytical chemistry ,Cytological Techniques ,02 engineering and technology ,Ion concentration polarization ,021001 nanoscience & nanotechnology ,Isolation (microbiology) ,Exosomes ,01 natural sciences ,Exosome ,Microvesicles ,General Biochemistry, Genetics and Molecular Biology ,0104 chemical sciences ,Cell biology ,chemistry ,Cell-Derived Microparticles ,Ultracentrifuge ,0210 nano-technology - Abstract
Microvesicles and exosomes are small membranous vesicles released to the extracellular environment and circulated throughout the body. Because they contain various parental cell-derived biomolecules such as DNA, mRNA, miRNA, proteins, and lipids, their enrichment and isolation are critical steps for their exploitation as potential biomarkers for clinical applications. However, conventional isolation methods (e.g., ultracentrifugation) cause significant loss and damage to microvesicles and exosomes. These methods also require multiple repetitive steps of ultracentrifugation, loading, and wasting of reagents. This article describes a detailed method to fabricate an origami-paper-based device (Exo-PAD) designed for the effective enrichment and isolation of microvesicles and exosomes in a simple manner. The unique design of the Exo-PAD, consisting of accordion-like multifolded layers with convergent sample areas, is integrated with the ion concentration polarization technique, thereby enabling fivefold enrichment of the microvesicles and exosomes on specific layers. In addition, the enriched microvesicles and exosomes are isolated by simply unfolding the Exo-PAD.
- Published
- 2020
50. Call for Papers: 'In It for the Long Haul: Understanding the Lasting Impact of COVID-19 on Lung Health and Disease'
- Author
-
Nathan W. Bartlett, Julie A. Bastarache, Wolfgang M. Kuebler, and Eric P. Schmidt
- Subjects
Pulmonary and Respiratory Medicine ,Time Factors ,Physiology ,Physiology (medical) ,Humans ,COVID-19 ,Cell Biology ,Lung - Published
- 2022
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.