Search

Shades are adjusted in sweet pepper cultivation, based on solar exposure levels. Pyranometers and photosensitive films have recently been introduced to smart agriculture. However, there are no means of observing biological responses to solar exposure. In this study, we hypothesized that solar exposure levels affect the visible autofluorescence of sweet pepper under 365 nm illumination. To test this hypothesis, we cultivated sweet pepper plants under two exposure conditions, low (half of the normal) and high (the normal). Fluorescence photography (365 nm illumination) revealed that dark-fluorescent peppers only arise when cultivated under high-exposure conditions (0.7-fold decline at emission of 390 nm for high-exposure conditions). Microscopic and spectroscopic observations showed that blue autofluorescence was accompanied by an accumulation of UVB pigments (1.2-factor increase in the absorbance at 300 nm) and epidermal development (1.3-fold thicker cell wall). This study suggests that the autofluorescence of sweet pepper can possibly be used to understand the response of crop to solar radiation at a fruit level in horticulture., (© 2022. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.)

Verticillium dahliae is an economically relevant soilborne pathogen that causes vascular wilt in several crops, including pepper (Capsicum annuum). Fungal infection is usually visualized as a vascular browning, likely due to the onset of phenylpropanoid metabolism, which also seems to play a crucial role in the tolerance of some pepper varieties. In the current work, the potential function of distinct phenylpropanoid derivatives (suberin, lignin and phenolic compounds) in the pepper tolerance response against V. dahliae, was investigated. Histochemical and biochemical analyses ruled out suberin as a key player in the pepper-fungus interaction. However, changes observed in lignin composition and higher deposition of bound phenolics in infected stems seemed to contribute to the reinforcement of cell walls and the impairment of V. dahliae colonization. Most importantly, this is the first time that the accumulation of the hydroxycinnamic acid amide N-feruloyltyramine was reported in pepper stems in response to a vascular fungus. Fungitoxic activity for that hydroxycinnamate-tyramine conjugate was demonstrated as well., (Copyright © 2017 Elsevier B.V. All rights reserved.)