1. 7-Hydroxymatairesinol improves body weight, fat and sugar metabolism in C57BJ/6 mice on a high-fat diet.
- Author
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Biasiotto G, Zanella I, Predolini F, Archetti I, Cadei M, Monti E, Luzzani M, Pacchetti B, Mozzoni P, Andreoli R, De Palma G, Serana F, Smeds A, and Di Lorenzo D
- Subjects
- 3T3-L1 Cells, 4-Butyrolactone analogs & derivatives, 4-Butyrolactone pharmacology, 4-Butyrolactone therapeutic use, Adipogenesis drug effects, Adipogenesis genetics, Adipose Tissue cytology, Adipose Tissue drug effects, Adipose Tissue metabolism, Animals, Anti-Obesity Agents pharmacology, Anti-Obesity Agents therapeutic use, Dietary Supplements, Fatty Liver drug therapy, Fatty Liver metabolism, Gene Expression, Insulin Resistance, Lignans therapeutic use, Lipids blood, Male, Metabolic Syndrome blood, Metabolic Syndrome etiology, Metabolic Syndrome metabolism, Mice, Mice, Inbred C57BL, Obesity blood, Obesity etiology, Obesity metabolism, Obesity prevention & control, Plant Extracts pharmacology, Plant Extracts therapeutic use, Blood Glucose metabolism, Body Weight drug effects, Diet, High-Fat, Lignans pharmacology, Lipid Metabolism drug effects, Metabolic Syndrome drug therapy, Picea chemistry
- Abstract
7-Hydroxymatairesinol (7-HMR) is a plant lignan abundant in various concentrations in plant foods. The objective of this study was to test HMRLignan™, a purified form of 7-HMR, and the corresponding Picea abies extract (total extract P. abies; TEP) as dietary supplements on a background of a high-fat diet (HFD)-induced metabolic syndrome in mice and in the 3T3-L1 adipogenesis model. Mice, 3 weeks old, were fed a HFD for 60 d. Subgroups were treated with 3 mg/kg body weight 7-HMR (HMRLignan™) or 10 mg/kg body weight TEP by oral administration. 7-HMR and TEP limited the increase in body weight (-11 and -13 %) and fat mass (-11 and -18 %) in the HFD-fed mice. Epididymal adipocytes were 19 and -12 % smaller and the liver was less steatotic (-62 and -65 %). Serum lipids decreased in TEP-treated mice (-11 % cholesterol, -23 % LDL and -15 % TAG) and sugar metabolism was ameliorated by both lignan preparations, as shown by a more than 70 % decrease in insulin secretion and insulin resistance. The expression of several metabolic genes was modulated by the HFD with an effect that was reversed by lignan. In 3T3-L1 cells, the 7-HMR metabolites enterolactone (ENL) and enterodiol (END) showed a 40 % inhibition of cell differentiation accompanied by the inhibited expression of the adipogenic genes PPARγ, C/EBPα and aP2. Furthermore, END and ENL caused a 10 % reduction in TAG uptake in HEPA 1-6 hepatoma cells. In conclusion, 7-HMR and TEP reduce metabolic imbalances typical of the metabolic syndrome and obesity in male mice, whereas their metabolites inhibit adipogenesis and lipid uptake in vitro.
- Published
- 2018
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