1. Tailoring the whole-cell sensing spectrum with cyborgian redox machinery.
- Author
-
Yang, Kai, Lu, Zi-Jie, Zhu, Tian-Yu, Wang, Jing-Xian, Yang, Fu-Qiao, Abbas, Syed Zaghum, Zhou, Jun, Yang, Zhugen, Mi, Jian-Li, Ravi, Sai Kishore, and Yong, Yang-Chun
- Subjects
- *
SHEWANELLA oneidensis , *OXIDATION-reduction reaction , *VITAMIN B12 , *CHARGE exchange , *MACHINERY , *BIOELECTROCHEMISTRY - Abstract
Whole-cell biosensors are an important class of analytical tools that offer the advantages of low cost, facile operation, and unique reproduction/regeneration ability. However, it has always been quite challenging to expand the sensing spectrum of the host. Here, a new approach to extend the cell sensing spectrum with biomineralized nanoparticles is developed. The nano-biohybrid design comprise biomineralized FeS nanoparticles firmly anchored onto the bacterium, Shewanella oneidensis MR-1, wherein the nanoparticles are wired to the cellular electron transfer machinery (MtrCAB/OmcA) of the bacterium, forming an artificial cyborgian redox machinery consisting of FeS-MtrCAB/OmcA-FccA. Strikingly, with this cyborgian redox machinery, the sensing spectrum of FeS hybridized S. oneidensis MR-1 cell is successfully expanded to enable whole-cell electrochemical detection of Vitamin B12, while an unhybridized native cell is incapable of sensing. This proof-of-concept nano-biohybrid design offers a new perspective on manipulating the microbial toolkit for an expanded sensing spectrum in whole-cell biosensors. [Display omitted] • A nano-hybridized electroactive bacteria based system was assembled. • Artificial cyborgian redox machinery was identified in this biohybrid cell. • This cyborgian redox machinery expanded the sensing spectrum of Shewanella cell. • A sensitive whole-cell biosensing system for VB12 was developed. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF