1. Cytogenetic maps of homoeologous chromosomes A h01 and D h01 and their integration with the genome assembly in Gossypium hirsutum
- Author
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Zhang Zhenmei, Renhai Peng, Zhongli Zhou, Xiaoyan Cai, Yuhong Wang, Xingxing Wang, Zhen Liu, Fang Liu, Yuling Liu, and Kunbo Wang
- Subjects
Malvales ,0301 basic medicine ,lcsh:QH426-470 ,Gossypium hirsutum ,Sequence assembly ,physical map ,Genomics ,Plant Science ,Gossypium ,cotton ,Magnoliopsida ,03 medical and health sciences ,FISH ,Genetic linkage ,Genetics ,medicine ,draft genome assembly ,Plantae ,Malvaceae ,BAC ,Bacterial artificial chromosome ,medicine.diagnostic_test ,biology ,Chromosome ,biology.organism_classification ,Tracheophyta ,lcsh:Genetics ,030104 developmental biology ,Animal Science and Zoology ,Biotechnology ,Fluorescence in situ hybridization ,Research Article - Abstract
Cytogenetic maps of Gossypium hirsutum (Linnaeus, 1753) homoeologous chromosomes Ah01 and Dh01 were constructed by fluorescence in situ hybridization (FISH), using eleven homoeologous-chromosomes-shared bacterial artificial chromosomes (BACs) clones and one chromosome-specific BAC clone respectively. We compared the cytogenetic maps with the genetic linkage and draft genome assembly maps based on a standardized map unit, relative map position (RMP), which allowed a global view of the relationship of genetic and physical distances along each chromosome, and assembly quality of the draft genome assembly map. By integration of cytogenetic maps with sequence maps of the two chromosomes (Ah01 and Dh01), we inferred the locations of two scaffolds and speculated that some homologous sequences belonging to homoeologous chromosomes were removed as repetitiveness during the sequence assembly. The result offers molecular tools for cotton genomics research and also provides valuable information for the improvement of the draft genome assembly.
- Published
- 2017