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The objective was to evaluate the effects of In vitro biocontrol of Trichoderma spp. and Bacillus spp. against Fusarium oxysporum and its in vivo effect on Solanum lycopersicum plants. The antagonistic effect in vitro of microcapsules of Trichoderma spp. and Bacillus spp. against F. oxysporum, was evaluated by inhibition and antagonism classification, in the in vivo part growth variables in plant and fruit were measured. Trichoderma asperellum showed inhibition of 84.7% and class 1 and Bacillus spp., obtained an inhibition of 83.7% with a positive classification. The canonical correspondence analysis of the fruit, the T1 treatment indicates that the variables that highlight the effect of the treatment are the firmness of the fruit and the Brix. The canonical correspondence analysis of the plant, the height presents a significant difference (P < 0.05), highlighting B. subtilis, Bacillus liquefasciens, T. harzianum and T. asperellum + Bacillus amiloliquefasciens, with 1.05 and 1.1 m compared to the control of 74 cm, also highlighted the number of total fruits in the T. harzianum treatment with an average of 22.6 fruits and root weight in B. subtilis with 37.12 g. Strains of Trichoderma spp. and Bacillus spp. successfully inhibited F. oxysporum also induced growth in the tomato plant. [ABSTRACT FROM AUTHOR]

The objective was to evaluate the antagonistic effect in vitro of native strains of Trichoderma asperellum, Trichoderma harzianum and two isolates of Bacillus spp. against Fusarium oxysporum and Dickeya chrysanthemi. Bacillus, isolated from soil samples of Aloe vera plantations, was morphologically identified and a modified dual culture confrontation was carried out, placing the F. oxysporum in the center of the Petri dish and Bacillus in the four cardinal points; it was arranged under a completely randomized experimental design with five repetitions, the variables registered were percentage of antagonism, inhibition halo and concentration of conidia. For Trichoderma they were confronted against F. oxysporum and D. chrysanthemi with a dual confrontation, a percentage of inhibition and antagonism classification were determined. In the confrontation of T. asperellum it obtained a percentage of inhibition of 70.5% against F. oxysporum and 41.9% against D. chrysanthemi. Regarding the inhibition halos of Bacillus (B5 and B4), they obtained 4 mm. In the conidia concentration B5 showed 1.3, B4 2.6 and the control 12.6 spores per dilution. Trichoderma and Bacillus represent a viable alternative for the control of F. oxysporum and D. chrysanthemi. [ABSTRACT FROM AUTHOR]