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Start Over Topic aspartic acid Publication Year Range Last 10 years
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1. Colorimetric determination of cysteine by a paper-based assay system using aspartic acid modified gold nanoparticles.

Author

Liu C, Miao Y, Zhang X, Zhang S, and Zhao X

Subjects
Carbohydrate Sequence, Cell Phone, Colorimetry instrumentation, Gold chemistry, Humans, Limit of Detection, Software, Aspartic Acid chemistry, Colorimetry methods, Cysteine blood, Metal Nanoparticles chemistry, Paper
Abstract

A method is described for cysteine (Cys) determination on paper-based analytical devices using aspartic acid modified gold nanoparticles (Asp-AuNPs). The Asp-AuNPs were characterized by their size, zeta potential, and UV-visible absorption spectrum. After the addition of Cys, it will interact with Asp-AuNPs selectively and leads to the aggregation of Asp-AuNPs. A color change from red to blue can be observed on the paper-based analytical devices. The results were recorded using a cell phone and subsequently analyzed using the Photoshop software. The ratiometric color intensity at red channel and blue channel (Red/Blue) increased linearly in the range 99.9-998.7 μM for Cys (R = 0.9984), and the limit of detection was 1.0 μM. The effects of assay conditions have been investigated and are discussed. The Cys concentration was determined as (0.27 ± 0.02 mM) in human plasma, and the recovery was from 99.2 to 101.1%. Graphical abstract Schematic representation of the paper-based assay system using aspartic acid modified gold nanoparticles (Asp-AuNPs). The ratiometric color intensity method was used for the cysteine (Cys) determination.

Published
2020
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2. Colorimetric determination of cysteine by a paper-based assay system using aspartic acid modified gold nanoparticles

Author

Xuejiao Zhang, Chunye Liu, Xiaojun Zhao, Shuli Zhang, and Yanqing Miao

Subjects
Paper, Absorption spectroscopy, Nanochemistry, Metal Nanoparticles, 02 engineering and technology, 01 natural sciences, Analytical Chemistry, Limit of Detection, Aspartic acid, Zeta potential, Humans, Cysteine, Detection limit, Aspartic Acid, Chemistry, 010401 analytical chemistry, Paper based, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Carbohydrate Sequence, Colloidal gold, Colorimetry, Gold, 0210 nano-technology, Cell Phone, Software, Nuclear chemistry
Abstract

A method is described for cysteine (Cys) determination on paper-based analytical devices using aspartic acid modified gold nanoparticles (Asp-AuNPs). The Asp-AuNPs were characterized by their size, zeta potential, and UV-visible absorption spectrum. After the addition of Cys, it will interact with Asp-AuNPs selectively and leads to the aggregation of Asp-AuNPs. A color change from red to blue can be observed on the paper-based analytical devices. The results were recorded using a cell phone and subsequently analyzed using the Photoshop software. The ratiometric color intensity at red channel and blue channel (Red/Blue) increased linearly in the range 99.9-998.7 μM for Cys (R = 0.9984), and the limit of detection was 1.0 μM. The effects of assay conditions have been investigated and are discussed. The Cys concentration was determined as (0.27 ± 0.02 mM) in human plasma, and the recovery was from 99.2 to 101.1%. Graphical abstract Schematic representation of the paper-based assay system using aspartic acid modified gold nanoparticles (Asp-AuNPs). The ratiometric color intensity method was used for the cysteine (Cys) determination.

Published
2019