1. Recombinant production, purification and characterization of vessel dilator in E. coli.
- Author
-
Abbasian M, Eslampanah Seyedi HA, Sayed Tabatabaei BE, Arab-Bafrani Z, Mofid MR, and Zareie R
- Subjects
- Cell Line, Tumor, Chromatography, Affinity, Colorectal Neoplasms metabolism, Colorectal Neoplasms pathology, Escherichia coli genetics, Histidine biosynthesis, Histidine isolation & purification, Humans, Peptide Fragments biosynthesis, Peptide Fragments genetics, Peptide Fragments isolation & purification, Peptide Fragments pharmacology, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins isolation & purification, Recombinant Fusion Proteins pharmacology, Antineoplastic Agents isolation & purification, Antineoplastic Agents metabolism, Antineoplastic Agents pharmacology, Atrial Natriuretic Factor biosynthesis, Atrial Natriuretic Factor genetics, Atrial Natriuretic Factor isolation & purification, Atrial Natriuretic Factor pharmacology, Colorectal Neoplasms drug therapy, Escherichia coli metabolism
- Abstract
Vessel dilator is a 3.9-KDa potent anticancer peptide and a valuable candidate in the treatment of conditions such as congestive heart failure and acute renal failure amongst others. Here we report the recombinant production of vessel dilator in Escherichia coli. Three different synthetic ORF's dubbed VDI, VDII and VDIII, each encoding a trimmer of the vessel dilator peptide attached to a His tag sequence at their C- terminal, were synthesized and placed in pET21c expression vectors. The highest yield, following expression in E. coli BL21 (DE3), was recorded with VDII that carried the shortest fusion partner. Subsequent to the initial capture of the fusion protein by a Ni affinity column, the vessel dilator monomers were cleaved by trypsin treatment, and further purified to at least 90% homogeneity by anion exchange chromatography. De-novo sequencing and in vivo anticancer activity tests were used to verify the peptide sequence and its biological activity, respectively. The final yield was estimated to be approximately 15 mg of the purified vessel dilator per gram wet weight of the bacterial cells., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2017
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