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To investigate whether incorporation of small peptides into diets could change the growth performance, muscle quality and liver health of the largemouth bass, four diets were designed: SP0+ group (positive control group) was formulated according to the nutritional requirement of the largemouth bass. In SP0− group (negative control group), the crude protein level was decreased by 30 g/kg compared with SP0+ group; SP20 group was added 20 g/kg small peptides based on the SP0− group, and its crude protein level did not reach that of SP0+ group. In SP65 group, 65 g/kg small peptides were added on the basis of SP0− group to obtain same crude protein level as SP0+ group. Fish were fed with these four different diets for 9 weeks. Results showed that, compared with the negative control group, the addition of 20 g/kg small peptides had no significant effect on the weight gain rate and feed coefficient of the largemouth bass; adding 65 g/kg small peptides could significantly increase the weight gain rate and reduce the feed coefficient (p <.05), both reaching the level of the positive control group. Adding 20 g/kg or/and 65 g/kg of small peptides could significantly increase the content of cysteine and proline, the total amino acid, essential amino acid and non‐essential amino acid in the muscle (p <.05), as well as fatty acids, especially ARA, EPA and DHA. Adding 20 g/kg or 65 g/kg small peptides to the feed could increase the protease activity of the digestive system and apparent protein digestibility while increasing liver and serum's antioxidant enzyme activity to control the negative effect of MDA. Because total superoxide dismutase and catalase protected fish from oxidation, muscle quality and live health were both improved, consequently enhanced transaminase in the liver and reduced transaminase in the serum were observed. [ABSTRACT FROM AUTHOR]

This study aimed to explore the phylogenetic and molecular characteristics of feline astrovirus. A total of 33 fecal samples of domestic cats with or without diarrhea were collected from the Anhui province, and two positive samples were detected. The complete genome and ORF2 of the two strains were sequenced and phylogenetically analyzed. AH-1-2020 and AH-2-2020 displayed 83.4% homology, and their homologies with other reference strains were 75.3%–83.4% and 83.4%–95.0%, respectively. Phylogenetic tree analysis revealed that all strains could be classified into three different clusters; therefore, the mean amino acid genetic distances (p-dist) among the three clusters were estimated. The results suggested that the two strains and other FeAstV strains were grouped into three genotypes, with AH-1-2020 belonging to a novel genotype. High similarity was observed (65.9%–66.5% nucleotide identity and 63.8%–64.8% amino acid identity) in ORF2 between porcine astrovirus type 1 and AH-1-2020. Furthermore, inter-specific recombination between porcine astrovirus type 1 and FeAstV was observed. We, therefore, inferred that inter-specific transmission may exist between pigs and cats; however, further studies are required to verify this. This is the first report on the genetic characterization and phylogenetic analysis of FeAstVs in the Anhui province and would further the current understanding of the genetic diversity and epidemiology of FeAstVs. [ABSTRACT FROM AUTHOR]

Amino acid functionalized D301 resins (Tyr-D301, Phe-D301, and Ala-D301) are successfully prepared to remove phenols. The influences of adsorption condition on adsorption ability and the practical application value are investigated. The functionalized D301 resins possess strong adsorption ability toward phenol by ways of hydrogen bond interaction. The adsorption capacity of Tyr-D301, Phe-D301, and Ala-D301 is 388.2, 368.4, and 321.5 mg· g−1 at 293 K and pH of 2, respectively. The adsorption process could be described satisfactorily by Lagergren-first-order model and Langmuir model. The rate constant is 0.057, 0.042, and 0.035 min−1, respectively. The functionalized D301 can be used to dispose wastewater containing phenols. [ABSTRACT FROM AUTHOR]