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Objective: Food insecurity in college students is associated with poor eating habits. The purpose of this project was to increase consumption of fruits and vegetables in college students using a campus food pantry. Participants: Twenty-nine college students completed the study. Methods: Participants viewed weekly food preparation videos demonstrating cost-effective recipes containing fruits and vegetables and a key take-away message. Participants were provided a recipe and food to cook at home based on the Cooking Matters Food Pantry Toolkit. A 24-h dietary recall was collected pre- and postintervention using the Automated Self-Administered 24-H Dietary Assessment. This descriptive comparative study used repeated measure ANOVA. Results: Females (n = 19) increased their fruit and vegetable consumption by 1.3 cups daily (p < 0.05) while males (n = 10) decreased fruit and vegetable consumption at the postintervention measurement. Conclusion: Increased consumption of fruits and vegetables in female college students using the food pantry was a modifiable behavior in this study.

Indirect carbonation, a technology to store CO2 and produce stable CaCO3 and MgCO3, involves elution of Ca and Mg from industrial waste and subsequent carbonation. Although substantial residual waste is generated after the elution of Ca and Mg, its recycling attributes have not been adequately scrutinized. The residual waste has lower Ca and Mg contents and higher Si and Al contents than those of the raw material (i.e., industrial waste). This study involves the hydrothermal synthesis of zeolite-P using residual waste from indirect carbonation, conducted at both 100 and 180 ℃. The properties of these zeolites are compared with those synthesized from coal fly ash (CFA). The synthesized zeolites were characterized by X-ray diffraction, field emission scanning electron microscope, thermogravimetric analyzer, and Brunauer–Emmett–Teller (BET). A high zeolite conversion efficiency was achieved through a hydrothermal reaction (up to 87%), even though Si and Al were not added to the residual waste. Additionally, the cation exchange capacity and BET specific surface area of the synthesized zeolites were high (200 cmol kg−1 and 73 m2 g−1, respectively). These findings highlight the possibility of synthesizing zeolites using the residual waste from indirect carbonation as an alternative to conventional zeolite synthesis using industrial waste such as CFA. The synthesized zeolite-P is expected to be effective in wastewater treatment, detergent manufacturing, and water softening. [ABSTRACT FROM AUTHOR]

A low soybean self-sufficiency rate in South Korea has caused a high import dependence and considerable price variation between domestic and foreign soybeans, causing the false labeling of foreign soybeans as domestic. Conventional soybean origin discrimination methods prevent a single-grain analysis and rely on the presence or absence of several compounds or concentration differences. This limits the origin discrimination of mixed samples, demonstrating the need for a method that analyzes individual grains. Therefore, we developed a method for origin discrimination using genetic analysis. The whole-genome sequencing data of the Williams 82 reference cultivar and 15 soybean varieties cultivated in South Korea were analyzed to identify the dense variation blocks (dVBs) with a high single-nucleotide polymorphism density. The PCR primers were prepared and validated for the insertion–deletion (InDel) sequences of the dVBs to discriminate each soybean variety. Our method effectively discriminated domestic and foreign soybean varieties, eliminating their false labeling. [ABSTRACT FROM AUTHOR]

Background: Poor sleep among college students is a major, growing problem associated with lower academic performance, higher rates of health and emotional problems, and development of chronic sleep disorders. Purpose: Though previous studies have focused on individual colleges, our study purpose was to reveal the association of behaviors and function to sleep quality in a large sample of students from across the United States. Methods: We analyzed data from the Fall 2009 American College Health Association's National College Health Assessment-II survey using descriptive statistics, independent sample t tests, chi-square tests, and logistic regression to find a representative, matched sample of 14,870 college students, both with and without a sleep problem, measured by daytime sleepiness. Results: Our correlational analysis revealed that sleep quality decreased with increasing levels of stress or alcohol/cigarette use and with decreasing levels of general health (all Ps < 0.001). Discussion: College interventions should include efforts to address sleep quality to improve students' academic performance and long-term quality of life, especially for students experiencing substantial stress or poor general health. Translation to Health Education Practice: Educators, advisors, and leaders should incorporate sleep quality improvements into their services and programs for all students, especially students in at-risk groups.

Furfuryl alcohol, which is the hydrogenated product of furfural, has been identified as a very promising platform chemical with high potential for applications in the manufacture of key chemicals, lubricants, fragrances, and pharmaceuticals. In this work, bare SB, and x % Ru/Al‐SB (x=1.5, 2.5, 3.5, and 4.5 wt. %) samples were fabricated by a hydrothermal method. Bare and most active catalysts were characterized by different techniques, such as BET, FE‐SEM, TEM, FT‐IR, and XRD, to understand their physical and chemical properties. An evaluation of the effects of various reaction parameters, such as catalyst loading, reaction temperature, and reaction time, on the catalytic performance, showed higher catalytic conversion of furfural and selectivity for the desired products. The most active RuS3 catalyst showed 100 % conversion of furfural and 99 % selectivity for furfuryl alcohol. It could be reused for five consecutive reaction cycles without significant loss of performance. In addition, Ru leaching and loss of conversion or selectivity were not noticed during the five‐run recycling test. The EDS elemental mapping analysis of the used catalyst established the preservation of the mesoporous structure, suggesting a strong interaction between the hexagonal porous silicate and the Ru nanoparticles. [ABSTRACT FROM AUTHOR]

Stimulator of interferon genes (STING) is an adapter protein that is activated when cyclic dinucleotides (CDNs) are present. CDNs originate from the cytosolic DNA of both pathogens and hosts. STING activation promotes efficient immune responses against viral infections; however, its impact in bacterial infections is unclear. In this study, we investigated the role of Sting in bacterial infections by successfully creating a sting-deficient (sting(−/−) with a 4-bp deletion) knockout zebrafish model using CRISPR/Cas9. The transcriptional modulation of genes downstream of cGAS (cyclic GMP-AMP synthase)-Sting pathway-related genes was analyzed in seven-day-old wild-type (WT) and sting(−/−) embryos, as well as in four-day-old LPS-stimulated embryos. The expression of downstream genes was higher in sting(−/−) than in healthy WT fish. The late response was observed in sting(−/−) larvae following LPS treatment, demonstrating the importance of Sting-induced immunity during bacterial infection by activating the cGAS–STING pathway. Furthermore, adult sting(−/−) fish had a high mortality rate and significantly downregulated cGAS–STING pathway-related genes during Edwardsiella piscicida (E. piscicida) infection. In addition, we assessed NF-κB pathway genes following E. piscicida infection. Our results show fluctuating patterns of interleukin-6 (il6) and tumor necrosis factor-α (tnfα) expression, which is likely due to the influence of other NF-κB pathway-related immune genes. In summary, this study demonstrates the important role of Sting against bacterial infection. [ABSTRACT FROM AUTHOR]

Objective: Food insecurity in college students is associated with poor eating habits. The purpose of this project was to increase consumption of fruits and vegetables in college students using a campus food pantry. Participants: Twenty-nine college students completed the study. Methods: Participants viewed weekly food preparation videos demonstrating cost-effective recipes containing fruits and vegetables and a key take-away message. Participants were provided a recipe and food to cook at home based on the Cooking Matters Food Pantry Toolkit. A 24-h dietary recall was collected pre- and postintervention using the Automated Self-Administered 24-H Dietary Assessment. This descriptive comparative study used repeated measure ANOVA. Results: Females (n = 19) increased their fruit and vegetable consumption by 1.3 cups daily (p <.05) while males (n = 10) decreased fruit and vegetable consumption at the postintervention measurement. Conclusion: Increased consumption of fruits and vegetables in female college students using the food pantry was a modifiable behavior in this study. [ABSTRACT FROM AUTHOR]

Loss of L-gulonolactone oxidase (GULO), which catalyzes the last step of the ascorbic acid (AA) biosynthesis pathway, results in a complete lack of AA in several Osteichthyes fish species, including zebrafish. In this study, sGULO , the active GULO gene from cloudy catshark (Scyliorhinus torazame) was cloned into zebrafish using the Gateway cloning method. The resulting Tg(b-actin:sGULO:mCherry) fish were analyzed for the effects of a reestablished AA pathway. Fluorescent microscopy and PCR were used to analyze the integration of the construct into the zebrafish genome. Catalytic activity of sGULO, AA production, growth-related characteristics, and gene expression were investigated to evaluate the effects of AA production in Tg fish. The mCherry fluorescent protein indicated the proper integration and expression of the sGULO construct in zebrafish. The sGULO gene was ubiquitously expressed in all the studied tissues and the enzyme activity indicated an increased AA production in Tg fish. The growth of Tg fish was also increased, and antioxidant system analysis suggests that reactive oxygen species production was reduced in Tg fish compared with wild type. Expression of the AA transporter slc23a1 was significantly downregulated in Tg homozygous fish. These results collectively indicate the effects of reestablished AA synthesis in zebrafish. [ABSTRACT FROM AUTHOR]

The tetraspanin superfamily proteins are transmembrane proteins identified in a diverse range of eukaryotic organisms. Tetraspanins are involved in a variety of essential biological functions, including cell differentiation, adhesion, migration, signal transduction, intracellular trafficking, and immune responses. For an infection to occur, viruses must interact with various cell surface components, including receptors and signaling molecules. Tetraspanin CD63 is involved in the organization of the cell membrane and trafficking of cellular transmembrane proteins that interact with many viruses. In this study, the cd63 gene was characterized by studying its expression and function in a zebrafish model. The functional domains and structural features of Cd63, such as the Cys-Cys-Gly (CCG) motif in the large extracellular loop and cysteine residues, are conserved in zebrafish. We confirmed that cd63 was expressed in immune system organs, such as the axial vein and pronephric duct, during the embryonic development of zebrafish. To better understand the role of cd63 in the zebrafish immune system, we established cd63 -deficient zebrafish lines using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system. A 19 bp insertion mutation was generated in single guide RNA (sgRNA) target sequence of exon 3 of the cd63 gene, to create a pre-mature stop codon. We then analyzed the expression of cd63 -related genes cxcr4a and cxcr4b in wild type (WT) and cd63 -deficient zebrafish. We believe our study provides an important model that could be used to investigate the roles of cd63 in viral infection in vivo. • Characterization of cd63 from zebrafish (Danio rerio). • Expression analysis at the developmental stage of zebrafish by in situ hybridization. • The cd63 expression was significantly increased upon the VHSV infection. • Cd63 knockout zebrafish was generated using a CRISPR/Cas9 system. • Cd63 -related genes, cxcr4a and cxcr4b , were downregulated in cd63 knockout zebrafish. [ABSTRACT FROM AUTHOR]