1. Programmable formation of catalytic RNA triangles and squares by assembling modular RNA enzymes.
- Author
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Hiroki Oi, Daisuke Fujita, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, Shigeyoshi Matsumura, and Yoshiya Ikawa
- Subjects
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MOLECULAR structure of catalytic RNA , *OLIGOMERS , *TRIMERIZATION , *ATOMIC force microscopy , *TETRAHYMENA - Abstract
RNA is a biopolymer that is attractive for constructing nano-scale objects with complex structures. Threedimensional (3D) structures of naturally occurring RNAs often have modular architectures. The 3D structure of a group I (GI) ribozyme from Tetrahymena has a typical modular architecture, which can be separated into two structural modules ("P5 and P5abc). The fully active ribozyme can be reconstructed by assembling the two separately prepared modules through highly specific and strong assembly between "P5 ribozyme and P5abc RNA. Such non-covalent assembly of the two modules allows the design of polygonal RNA nano-structures. Through rational redesign of the parent GI ribozyme, we constructed variant GI ribozymes as unit RNAs for polygonal-shaped (closed) oligomers with catalytic activity. Programmed trimerization and tetramerization of the unit RNAs afforded catalytically active nano-sized RNA triangles and squares, the structures of which were directly observed by atomic force microscopy (AFM). [ABSTRACT FROM AUTHOR]
- Published
- 2017
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