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目的建立基于化学遗传的慢性应激导致抑郁症的小鼠模型，为优化慢性应激诱发抑郁症的动物建模方法提供参考。方法通过化学遗传的方法，对小鼠延髓腹外侧区头端（RVLM）进行持续的刺激，模拟机体对慢性应激的响应，系统观察造模和对照组小鼠的行为学、血压及主要炎症因子的变化情况。结果成功建立了小鼠模型。经腹腔向小鼠给药CNO 1 h后，造模组较对照组的舒张压与平均脉压显著上升。持续给药4周后，造模组较对照组的旷场实验的水平运动总距离、中央区进入总次数、中央区滞留时间和中央区域运动总距离这四项指标均显著下降；蔗糖偏好实验提示造模组存在快感缺失；转棒实验提示造模组运动协调能力显著减弱；同时，造模组心率、血压、炎性因子IFN-γ和抑炎因子IL-10水平等也较对照组显著上调。结论我们的模型在诱发抑郁症经典症状，及血压偏高、炎性因子释放紊乱等抑郁症伴随症状上均表现出较高的成功率和稳定性。这为优化慢性应激诱发抑郁症的动物建模效率和稳定性提供了有参考价值的科学依据。

目的 探讨高、低频重复经颅磁刺激（repetitive transcranial magnetic stimulation，rTMS）大脑初级运动 皮层（M1区）治疗卒中后上肢痉挛的临床疗效及差异。 方法 纳入我院神经内科和中医理疗科卒中后痉挛性偏瘫患者60例，随机分为高频rTMS组、低频 rTMS组和假刺激组各20例。高频rTMS组刺激患侧M1区，刺激频率为10 Hz，刺激强度为80%运动阈 值；低频rTMS组刺激健侧M1区，刺激频率为1 Hz，刺激强度为90%运动阈值；假刺激组接受相同噪声 与时间的假刺激治疗。所有患者均接受常规康复治疗。各组患者分别在治疗前及治疗8周后采用改良 Ashworth痉挛评定量表（modified Ashworth scale，MAS）、Fugl-Meyer运动功能评定量表中上肢运动功能 测试部分（upper extremities motor function test of Fugl-Meyer movement assessment，U-FMA）和改良 Barthel指数（modified Barthel index，MBI）进行评定。以上治疗均1次/日，5次/周，连续治疗8周。 结果 与治疗前相比，治疗后三组患者上肢MAS、U-FMA和MBI评分均有显著改善，差异均有统计学意 义（P＜0.01）；治疗后，高频rTMS组和低频rTMS组比假刺激组的上肢MAS、U-FMA和MBI评分改善更明 显，比较差异有统计学意义（P＜0.05）；但低频rTMS组和高频rTMS组上肢MAS、U-FMA和MBI评分比较， 差异无统计学意义。 结论 健侧低频与患侧高频rTMS刺激大脑M1区均可有效改善患者的临床症状和运动功能障碍。两 种经颅磁刺激方案临床疗效相当。 Abstract: Objective To investigate the clinical efficacy and differences of repetitive transcranial magnetic stimulation (rTMS) for high-frequency and low-frequency stimulation in the treatment of stroke upper limb spasticity. Methods A total of 60 patients from department of neurology and physiotherapy with spasticity were randomized to receive 10 Hz (n =20), 1 Hz (n =20) or sham (n =20) rTMS. The high-frequency rTMS group and the low-frequency rTMS group were given rTMS for 40 days in the ipsilesional and contralesional primary motor cortex. The stimulation frequency of the high-frequency rTMS was 10 Hz and the stimulation intensity was 80% motor threshold (MT). The stimulation frequency of the low-frequency rTMS was 1 Hz, 90% MT; the sham stimulation group received sham stimulation. All of participants received conventional rehabilitation. All the treatments were performed once a day, 5 times per week, and continued for 8 weeks. The modified Ashworth scale (MAS), upper extremities motor function test of Fugl-Meyer movement assessment (U-FMA) and modified Barthel index (MBI) were performed before and 8 weeks after treatment. Results After treatment, there was a significant improvement of MAS U-FMA and MBI scores after treatment in three groups (P 0.05). Conclusion Low-frequency stimulation of the contralesional and high-frequency stimulation of the ipsilesional M1 area can both effectively improve the clinical symptoms and motor function of the spastic patients. There was no significant difference for their clinical efficacy.

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Copyright of Coal Science & Technology (0253-2336) is the property of Coal Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)