4 results on '"Bardelli, Fabrizio"'
Search Results
2. Asbestos bodies count and morphometry in bulk lung tissue samples by non-invasive X-ray micro-tomography.
- Author
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Bardelli F, Brun F, Capella S, Bellis D, Cippitelli C, Cedola A, and Belluso E
- Subjects
- Aged, 80 and over, Female, Humans, Imaging, Three-Dimensional, Male, X-Ray Microtomography, Asbestos adverse effects, Lung diagnostic imaging, Lung pathology
- Abstract
The number of the Asbestos Bodies (AB), i.e. asbestos that developed an iron-protein coating during its permanence in biological tissues, is one of the most accessible markers of asbestos exposure in individuals. The approaches developed to perform AB count in biological tissues are based on the manual examination of tissue digests or histological sections by means of light or electron microscopies. Although these approaches are well established and relatively accessible, manual examination is time-consuming and can be reader-dependent. Besides, approximations are applied because of the limitations of 2D readings and to speed up manual counts. In addition, sample preparation using tissue digests require an amount of tissue that can only be obtained by invasive surgery or post-mortem sampling. In this paper, we propose a new approach to AB counting based on non-destructive 3D imaging, which has the potential to overcome most of the limitations of conventional approaches. This method allows automating the AB count and determining their morphometry distribution in bulk tissue samples (ideally non-invasive needle biopsies), with minimal sample preparation and avoiding approximations. Although the results are promising, additional testing on a larger number of AB-containing biological samples would be required to fully validate the method.
- Published
- 2021
- Full Text
- View/download PDF
3. New insights on the biomineralisation process developing in human lungs around inhaled asbestos fibres.
- Author
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Bardelli F, Veronesi G, Capella S, Bellis D, Charlet L, Cedola A, and Belluso E
- Subjects
- Aged, 80 and over, Biopsy, Female, Humans, Iron, Lung metabolism, Lung pathology, Male, Metals, Heavy, Trace Elements, Asbestos adverse effects, Asbestosis etiology, Asbestosis pathology, Calcinosis metabolism, Mineral Fibers adverse effects, Occupational Exposure adverse effects
- Abstract
Once penetrated into the lungs of exposed people, asbestos induces an in vivo biomineralisation process that leads to the formation of a ferruginous coating embedding the fibres. The ensemble of the fibre and the coating is referred to as asbestos body and is believed to be responsible for the high toxicological outcome of asbestos. Lung tissue of two individuals subjected to prolonged occupational exposure to crocidolite asbestos was investigated using synchrotron radiation micro-probe tools. The distribution of K and of elements heavier than Fe (Zn, Cu, As, and Ba) in the asbestos bodies was observed for the first time. Elemental quantification, also reported for the first time, confirmed that the coating is highly enriched in Fe (~20% w/w), and x-ray absorption spectroscopy indicated that Fe is in the 3+ oxidation state and that it is present in the form of ferritin or hemosiderin. Comparison of the results obtained studying the asbestos bodies upon removing the biological tissue by chemical digestion and those embedded in histological sections, allowed unambiguously distinguishing the composition of the asbestos bodies, and understanding to what extent the digestion procedure altered their chemical composition. A speculative model is proposed to explain the observed distribution of Fe.
- Published
- 2017
- Full Text
- View/download PDF
4. Mesothelioma: Scientific clues for prevention, diagnosis, and therapy.
- Author
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Carbone, Michele, Adusumilli, Prasad S, Alexander, H Richard, Baas, Paul, Bardelli, Fabrizio, Bononi, Angela, Bueno, Raphael, Felley-Bosco, Emanuela, Galateau-Salle, Francoise, Jablons, David, Mansfield, Aaron S, Minaai, Michael, de Perrot, Marc, Pesavento, Patricia, Rusch, Valerie, Severson, David T, Taioli, Emanuela, Tsao, Anne, Woodard, Gavitt, Yang, Haining, Zauderer, Marjorie G, and Pass, Harvey I
- Subjects
Pleura ,Humans ,Mesothelioma ,Pleural Neoplasms ,Genetic Predisposition to Disease ,Asbestos ,Ubiquitin Thiolesterase ,Tumor Suppressor Proteins ,Diagnostic Errors ,Prognosis ,Combined Modality Therapy ,Pneumonectomy ,Incidence ,Inhalation Exposure ,Occupational Exposure ,Germ-Line Mutation ,International Cooperation ,United States ,Australia ,Europe ,Molecular Targeted Therapy ,Carcinogenesis ,Biomarkers ,Tumor ,Global Burden of Disease ,Antineoplastic Agents ,Immunological ,BRCA1-associated protein 1 ,asbestos ,cancer syndromes ,chromothripsis ,gene-environment interaction ,immunotherapy ,mesothelioma ,Lung ,Rare Diseases ,Lung Cancer ,Cancer ,Prevention ,Good Health and Well Being ,Clinical Sciences ,Public Health and Health Services ,Oncology & Carcinogenesis - Abstract
Mesothelioma affects mostly older individuals who have been occupationally exposed to asbestos. The global mesothelioma incidence and mortality rates are unknown, because data are not available from developing countries that continue to use large amounts of asbestos. The incidence rate of mesothelioma has decreased in Australia, the United States, and Western Europe, where the use of asbestos was banned or strictly regulated in the 1970s and 1980s, demonstrating the value of these preventive measures. However, in these same countries, the overall number of deaths from mesothelioma has not decreased as the size of the population and the percentage of old people have increased. Moreover, hotspots of mesothelioma may occur when carcinogenic fibers that are present in the environment are disturbed as rural areas are being developed. Novel immunohistochemical and molecular markers have improved the accuracy of diagnosis; however, about 14% (high-resource countries) to 50% (developing countries) of mesothelioma diagnoses are incorrect, resulting in inadequate treatment and complicating epidemiological studies. The discovery that germline BRCA1-asssociated protein 1 (BAP1) mutations cause mesothelioma and other cancers (BAP1 cancer syndrome) elucidated some of the key pathogenic mechanisms, and treatments targeting these molecular mechanisms and/or modulating the immune response are being tested. The role of surgery in pleural mesothelioma is controversial as it is difficult to predict who will benefit from aggressive management, even when local therapies are added to existing or novel systemic treatments. Treatment outcomes are improving, however, for peritoneal mesothelioma. Multidisciplinary international collaboration will be necessary to improve prevention, early detection, and treatment.
- Published
- 2019
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