41 results on '"Huang, Chi-Jung"'
Search Results
2. Colorectal cancer concurrent gene signature based on coherent patterns between genomic and transcriptional alterations
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Shen, Ming-Hung, Huang, Chi-Jung, Ho, Thien-Fiew, Liu, Chih-Yi, Shih, Ying-Yih, Huang, Ching-Shui, and Huang, Chi-Cheng
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- 2022
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3. Hydrostatic pressure facilitates calcium deposition and osteogenic gene expression in the osteoblastic differentiation of placenta-derived multipotent cells
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Cheng, Chih-Chien, Chung, Chih-Ang, Chang, Chih-Ju, Cheng, Yu-Che, Huang, Chi-Jung, Chien, Chih-Cheng, and Lin, Hsi-Ting
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- 2022
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4. Improvement of clinical outcomes in patients undergoing peritoneal dialysis using hydroxymethylglutaryl-CoA reductase inhibitors: A systematic review and meta-analysis
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Lee, Dan-Ying, Huang, Chi-Jung, Yeh, Wan-Yu, Sung, Shih-Hsien, Chen, Chen-Huan, and Cheng, Hao-Min
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- 2023
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5. Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression
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Huang, Chi-Cheng, Shen, Ming-Hung, Chen, Shao-Kuan, Yang, Shung-Haur, Liu, Chih-Yi, Guo, Jiun-Wen, Chang, Kang-Wei, and Huang, Chi-Jung
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- 2020
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6. The prognostic significance of the alterations of pulmonary hemodynamics in patients with pulmonary arterial hypertension: a meta-regression analysis of randomized controlled trials
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Sung, Shih-Hsien, Yeh, Wan-Yu, Chiang, Chern-En, Huang, Chi-Jung, Huang, Wei-Ming, Chen, Chen-Huan, and Cheng, Hao-Min
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- 2021
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7. MitoTox: a comprehensive mitochondrial toxicity database
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Lin, Yu-Te, Lin, Ko-Hong, Huang, Chi-Jung, and Wei, An-Chi
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- 2021
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8. Multi-gene signature of microcalcification and risk prediction among Taiwanese breast cancer
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Tsai, Hsin-Tien, Huang, Ching-Shui, Tu, Chao-Chiang, Liu, Chih-Yi, Huang, Chi-Jung, Ho, Yuan-Soon, Tu, Shih-Hsin, Tseng, Ling-Ming, and Huang, Chi-Cheng
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- 2020
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9. Propolis Has an Anticancer Effect on Early Stage Colorectal Cancer by Affecting Epithelial Differentiation and Gut Immunity in the Tumor Microenvironment.
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Shen, Ming-Hung, Liu, Chih-Yi, Chang, Kang-Wei, Lai, Ching-Long, Chang, Shih-Chang, and Huang, Chi-Jung
- Abstract
Colorectal cancer (CRC) is one of the most common cancers and is the second leading cause of cancer-related death in the world. Due to the westernization of diets, young patients with CRC are often diagnosed at advanced stages with an associated poor prognosis. Improved lifestyle choices are one way to minimize CRC risk. Among diet choices is the inclusion of bee propolis, long recognized as a health supplement with anticancer activities. Understanding the effect of propolis on the gut environment is worth exploring, and especially its associated intratumoral immune changes and its anticancer effect on the occurrence and development of CRC. In this study, early stage CRC was induced with 1,2-dimethylhydrazine (DMH) and dextran sulfate sodium (DSS) for one month in an animal model, without and with propolis administration. The phenotypes of early stage CRC were evaluated by X-ray microcomputed tomography and histologic examination. The gut immunity of the tumor microenvironment was assessed by immunohistochemical staining for tumor-infiltrating lymphocytes (TILs) and further comparative quantification. We found that the characteristics of the CRC mice, including the body weight, tumor loading, and tumor dimensions, were significantly changed due to propolis administration. With further propolis administration, the CRC tissues of DMH/DSS-treated mice showed decreased cytokeratin 20 levels, a marker for intestinal epithelium differentiation. Additionally, the signal intensity and density of CD3
+ and CD4+ TILs were significantly increased and fewer forkhead box protein P3 (FOXP3) lymphocytes were observed in the lamina propria. In conclusion, we found that propolis, a natural supplement, potentially prevented CRC progression by increasing CD3+ and CD4+ TILs and reducing FOXP3 lymphocytes in the tumor microenvironment of early stage CRC. Our study could suggest a promising role for propolis in complementary medicine as a food supplement to decrease or prevent CRC progression. [ABSTRACT FROM AUTHOR]- Published
- 2023
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10. Effectiveness of salt substitute on cardiovascular outcomes: A systematic review and meta-analysis.
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Tsai, Yi‐Ching, Tsao, Yen‐Po, Huang, Chi‐Jung, Tai, Yen‐Hsuan, Su, Yang‐Chin, Chiang, Chern‐En, Sung, Shih‐Hsien, Chen, Chen‐Huan, Cheng, Hao‐Min, Tsai, Yi-Ching, Tsao, Yen-Po, Huang, Chi-Jung, Tai, Yen-Hsuan, Su, Yang-Chin, Chiang, Chern-En, Sung, Shih-Hsien, Chen, Chen-Huan, and Cheng, Hao-Min
- Abstract
Hypertension-related death is the leading cause of mortality worldwide, making blood pressure (BP) control an important issue. Salt substitute is a non-pharmaceutical strategy to improve hypertension control. The goal of this study was to evaluate the effect of salt substitute on BP and cardiovascular disease. The authors searched the Cochrane Library and PubMed databases through March 2022, and assessed the risk-of-bias for included studies by the Cochrane risk-of-bias tool. Twenty-three randomized controlled trials with 32073 patients were included in our systematic review. A meta-analysis with random effects was performed to analyze the effects of salt substitute on systolic and diastolic BP, 24-h urinary sodium and potassium, and cardiovascular and all-cause mortality. In the random-effects model, participants consuming salt substitute showed significant reduction in systolic BP (mean difference (MD) -4.80 mmHg, 95% confidence interval (CI) -6.12 to -3.48, P < 0.0001) and diastolic BP (MD -1.48 mmHg, 95% CI -2.06 to -0.90, P < 0.0001) compared with participants consuming normal salt. In the urine electrolyte analysis, the salt substitute group had significant reduction in 24-h urine sodium (MD -22.96 mmol/24-h, P = 0.0001) and significant elevation in 24-h urine potassium (MD 14.41 mmol/24-h, P < 0.0001). Of the five studies with mortality outcome data, salt substitute significantly reduced all-cause mortality (hazard ratio 0.88, P = 0.0003). In conclusion, our analyses showed that salt substitute has a strong effect on lowering BP and reducing all-cause mortality. By modifying the daily diet with salt substitute, the authors can improve BP control by using this non-pharmaceutical management. [ABSTRACT FROM AUTHOR]
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- 2022
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11. 130/80 mmHg as a unifying hypertension threshold for office brachial, office central, and ambulatory daytime brachial blood pressure.
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Chuang, Shao‐Yuan, Cheng, Hao‐Min, Chang, Wei‐Lun, Yeh, Wan‐Yu, Huang, Chi‐Jung, and Chen, Chen‐Huan
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The present study investigated the prognostic values for office brachial (OB), office central (OC), and ambulatory daytime brachial (AmDB) hypertension, as defined by a unifying threshold of 130/80 mmHg, and the incremental value of either OC or AmDB hypertension to OB hypertension. A total of 1219 community residents without receiving anti‐hypertensive treatment (671 men and 548 women, aged ≥ 30 years old) from central Taiwan and Kinmen islands had OB, OC, and AmDB blood pressure measurements during a cardiovascular survey conducted in 1992–1993. OB hypertension, OC hypertension, and AmDB hypertension were all defined in retrospect at the threshold of 130/80 mmHg. They were followed up for nonfatal and fatal cardiovascular events until December 31, 2017, by linking the baseline database to the National Health Insurance Research dataset and the National Death Registry. During a follow‐up of 25 612.5 person‐years (Average event‐free time: 21.0 years), there were 368 fatal and nonfatal cardiovascular events. In multivariable analyses, OB hypertension, OC hypertension, and AmDB hypertension had similar hazard ratios for cardiovascular events [2.03, 95% confidence interval: 1.47‐2.80]; 1.92 (1.47‐2.51); and 1.79 (1.41‐2.29), respectively. Using OB normotension as the reference, either the concordant OB and OC hypertension [2.24 (1.61‐3.12)], or the concordant OB and AmDB hypertension [2.52 (1.80‐3.54)] was significantly associated with cardiovascular events. Moreover, OB hypertension plus AmDB normotension was also significantly associated with increased risk for cardiovascular events. We concluded that OB hypertension, OC hypertension, and AmDB hypertension defined by a unifying threshold of 130/80 mmHg may provide similar estimates of long‐term risk for cardiovascular events. Cross‐classification analyses suggest that addition of OC hypertension or AmDB hypertension may improve the prognostic value of OB hypertension. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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12. Functional Plasmon-Activated Water Increases Akkermansia muciniphila Abundance in Gut Microbiota to Ameliorate Inflammatory Bowel Disease.
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Chang, Chun-Chao, Liu, Chih-Yi, Su, I-Chia, Lee, Yuarn-Jang, Yeh, Hsing-Jung, Chen, Wen-Chao, Yu, Chih-Jui, Kao, Wei-Yu, Liu, Yu-Chuan, and Huang, Chi-Jung
- Subjects
INFLAMMATORY bowel diseases ,GUT microbiome ,PREBIOTICS ,SULFONIC acids - Abstract
Inflammatory bowel disease (IBD) is associated with dysbiosis and intestinal barrier dysfunction, as indicated by epithelial hyperpermeability and high levels of mucosal-associated bacteria. Changes in gut microbiota may be correlated with IBD pathogenesis. Additionally, microbe-based treatments could mitigate clinical IBD symptoms. Plasmon-activated water (PAW) is known to have an anti-inflammatory potential. In this work, we studied the association between the anti-inflammatory ability of PAW and intestinal microbes, thereby improving IBD treatment. We examined the PAW-induced changes in the colonic immune activity and microbiota of mice by immunohistochemistry and next generation sequencing, determined whether drinking PAW can mitigate IBD induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) and dysbiosis through mice animal models. The effects of specific probiotic species on mice with TNBS-induced IBD were also investigated. Experimental results indicated that PAW could change the local inflammation in the intestinal microenvironment. Moreover, the abundance of Akkermansia spp. was degraded in the TNBS-treated mice but elevated in the PAW-drinking mice. Daily rectal injection of Akkermansia muciniphila, a potential probiotic species in Akkermansia spp., also improved the health of the mice. Correspondingly, both PAW consumption and increasing the intestinal abundance of Akkermansia muciniphila can mitigate IBD in mice. These findings indicate that increasing the abundance of Akkermansia muciniphila in the gut through PAW consumption or other methods may mitigate IBD in mice with clinically significant IBD. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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13. Impaired renal function and mortalities in acute heart failure with different phenotypes.
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Huang, Wei‐Ming, Chang, Hao‐Chih, Lee, Ching‐Wei, Huang, Chi‐Jung, Yu, Wen‐Chung, Cheng, Hao‐Min, Guo, Chao‐Yu, Chiang, Chern‐En, Chen, Chen‐Huan, and Sung, Shih‐Hsien
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KIDNEY physiology ,HEART failure ,HEART failure patients ,SYSTOLIC blood pressure ,VENTRICULAR ejection fraction - Abstract
Aims: Impaired renal function (IRF) prevails in patients with acute heart failure. The study aimed to investigate the prevalence of on‐admission IRF and its association with short‐term and long‐term mortalities in patients hospitalized for HF with reduced (HFrEF), mildly reduced (HFmrEF), and preserved (HFpEF) left ventricular ejection fraction (LVEF). Methods: Patients hospitalized for acute heart failure were enrolled and stratified by LVEF into three phenotypes as HFpEF (≥50%), HFmrEF (40–49%), and HFrEF (<40%). IRF was defined as an estimated glomerular filtration rate of ≤60 mL/min/1.73m2 on admission. National Death Registry was linked for the identification of mortality. Results: Of 2613 patients enrolled, 673 (25.7%) had HFrEF, 367 (14.0%) had HFmrEF, and 1573 (60.1%) had HFpEF, whereas IRF was prevalent among 63.7, 68.6, and 67.5% of them, respectively. IRF significantly correlated with higher long‐term mortality in each phenotype of HF. However, IRF was associated with 90‐day and 1‐year mortality in subjects with HFrEF and HFmrEF, but not HFpEF. After accounting for age, gender, hypertension, diabetes, coronary artery disease, atrial fibrillation, stroke, serum sodium, de novo heart failure, date of enrolment, and systolic blood pressure <90 mmHg or use of inotropic agents, IRF remained related to 5‐year mortality in patients with HFrEF (hazard ratio and 95% confidence interval: 1.346, 1.034–1.751), HFmrEF (2.210, 1.435–3.404), and HFpEF (1.493, 1.237–1.801). Conclusions: On‐admission IRF was independently predictive of long‐term mortality in patients hospitalized for HF, irrespective of HF phenotypes. Furthermore, IRF was also associated with short‐term mortality in HFrEF and HFmrEF, but not in HFpEF. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Intestinal Mucosal Barrier Improvement with Prebiotics: Histological Evaluation of Longish Glucomannan Hydrolysates-Induced Innate T Lymphocyte Activities in Mice.
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Chang, Shih-Chang, Chiang, Hui-Hsun, Liu, Chih-Yi, Li, Yu-Ju, Lu, Chung-Lun, Lee, Yung-Pin, Huang, Chi-Jung, and Lai, Ching-Long
- Abstract
Use of prebiotics is a growing topic in healthcare. A lightweight molecule and water-soluble fiber ingredient, longish glucomannan hydrolysates (LGH), has been developed to improve the intestinal mucosal barrier and confer gut health benefits. This study aims to investigate the implications of continuous LGH intervening in intestinal epithelium integrity and protective immunity against chemical dextran sodium sulfate (DSS)-induced colitis. Twelve male BALB/c mice were randomly arranged into four groups. The LGH/DSS group had results in bodyweight variance, epithelial cell density, and aberrancy score as good as the LGH group, and both were equivalent to the control group. LGH consumption effectively protects the distal intestinal epithelium by activating innate T lymphocytes. Meanwhile, T-cell subsets in subepithelial interspersion take a bystander role in these microenvironmental alterations. Under this stress, the cluster of differentiation 3 (CD3)
+ T cells infiltrate the epithelium, while CD4+ T cells inversely appear in submucosal large lymphoid aggregates/isolated lymphoid follicles (ILFs) in which significant CD3+ , CD4+ , and CD8+ T-cell populations agglomerate. Moreover, forkhead box P3 (Foxp3) and interleukin 17 (IL-17) are observed in these ILFs. Agglomerated CD4+ T-cell lineages may have roles with proinflammatory T helper 17 cells and anti-inflammatory regulatory T cells in balancing responses to intraluminal antigens. Collectively, LGH administration may function in immune modulation to protect against DSS-induced inflammation. [ABSTRACT FROM AUTHOR]- Published
- 2022
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15. Effects of non-pharmacological coping strategies for reducing labor pain: A systematic review and network meta-analysis.
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Chang, Ching-Yi, Gau, Meei-Ling, Huang, Chi-Jung, and Cheng, Hao-min
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META-analysis ,LABOR (Obstetrics) ,PREGNANT women ,BAYESIAN analysis ,SMOOTH muscle contraction ,PSYCHOLOGICAL adaptation ,CHILDBIRTH - Abstract
Background: Facilitating the childbirth process is a global issue. Many strategies have been developed to cope with labor pain and improve the delivery experience and satisfaction of pregnant women. The results of different types of medical intervention on women's expectant pain have been varied. Therefore, this systematic review was aimed at summarizing the body of evidence regarding the effects of various non-pharmacological coping strategies for reducing labor pain. Methods: The review was conducted according to guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). We systematically searched the articles published between 1989 and 2020 in six electronic databases: PubMed, MEDLINE, CINAHL, WOS, PsycARTICLES, and Airiti Library, and the reference lists of the Clinical Trial Registry. Twenty studies were identified, with eight eligible studies included in the Bayesian network meta-analysis. Results: Eight studies with 713 participants were included in the meta-analysis with nine different non-pharmacological strategies for reducing labor pain. The traditional meta-analysis demonstrated that the non-pharmacological coping strategies were effective in reducing labor pain. Of these interventional strategies, the ranking probabilities analysis of the network meta-analysis suggested that the Bonapace Method may be the most effective strategy in reducing labor pain, followed by acupressure. Conclusions: Non-pharmacological coping strategies can reduce labor pain while maintaining an effective and satisfactory delivery experience. This systematic review, by synthesizing the body of evidence, demonstrated that non-pharmacological coping strategies are effective in reducing labor pain. Furthermore, as demonstrated in the network meta-analysis, the Bonapace Method, modulating birth pain by involving the father, is the most effective non-pharmacological intervention for reducing labor pain. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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16. Role of Heart Rate Variability in Association Between Glomerular Hyperfiltration and All-Cause Mortality.
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Hao-Chih Chang, Chi-Jung Huang, Yang, Albert C., Hao-Min Cheng, Shao-Yuan Chuang, Wen-Chung Yu, Chern-En Chiang, Chen-Huan Chen, Shih-Hsien Sung, Chang, Hao-Chih, Huang, Chi-Jung, Cheng, Hao-Min, Chuang, Shao-Yuan, Yu, Wen-Chung, Chiang, Chern-En, Chen, Chen-Huan, and Sung, Shih-Hsien
- Published
- 2021
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17. Lactobacillus rhamnosus GG as dietary supplement improved survival from lipopolysaccharides‐induced sepsis in mice.
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Tsui, Ko‐Chung, Yen, Ting‐Lin, Huang, Chi‐Jung, and Hong, Kun‐Jing
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LACTOBACILLUS rhamnosus ,DIETARY supplements ,SEPSIS ,SURVIVAL rate ,LABORATORY mice ,BACTERIOPHAGES - Abstract
Sepsis is a state of host immune response triggered by virus or bacterial infection, in which the extent of regional and systemic inflammation and companion counter‐inflammatory reactions determines disease outcomes. Probiotics are known for the immunomodulatory effect on allergic disorders, but it is not clear whether the beneficiary effect extends to sepsis and increases survival. In this mouse model, we injected intraperitoneally lipopolysaccharides (LPS) to induce sepsis, and investigated whether the pretreatment of Lactobacillus rhamnosus GG (LGG) contributed to host survival and examined the alteration of the gut microbiota and blood cytokines/chemokines profile before sepsis induction. Four‐week‐old male BALB/c mice were divided into two groups: one group were fed daily with LGG as a dietary supplement for fourteen days, whereas the other group with sterile water. Before sepsis induction, some mice from each group were killed to collect stool in the intestine and blood for microbial metagenomic and cytokine/chemokine analyses, respectively, and the rest were monitored afterward for mortality. The relative abundance of several families in the gut microbiota after LGG treatment was altered as well as the ratio of Firmicutes/Bacteroidetes. In addition, several pro‐inflammatory cytokines such as G‐CSF, IL7, IL15, and MCP1 were lower in the LGG group than in the control group. The survival rate following LPS‐induced sepsis improved with LGG treatment. Our results indicated that dietary supplement of probiotic LGG improved survival from LPS‐induced sepsis, most likely through pre‐septic changes in the gut microbial constituents by LGG with reciprocal alteration of host immune system to a less reactive state to incoming pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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18. Blood pressure variability and cognitive dysfunction: A systematic review and meta‐analysis of longitudinal cohort studies.
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Chiu, Tzu‐Jung, Yeh, Jiunn‐Tyng, Huang, Chi‐Jung, Chiang, Chern‐En, Sung, Shih‐Hsien, Chen, Chen‐Huan, and Cheng, Hao‐Min
- Abstract
The variability of blood pressure (BPV) has been suggested as a clinical indicator for cognitive dysfunction, yet the results from clinical studies are variable. This study investigated the relationship between BPV and the risk of cognitive decline or dementia. Bibliographic databases, including PubMed, Scopus, and Embase, were searched systematically for longitudinal cohort studies with BPV measurements and neuropsychological examinations or dementia diagnosis. A traditional meta‐analysis with subgroup analysis, and a further dose‐response meta‐analysis were conducted. Twenty cohort studies with 7 924 168 persons were included in this review. The results showed that a higher systolic BPV (SBPV), when measured with the coefficient of variation (SBP‐CV) or standard deviation (SBP‐SD), was associated with a higher risk of all‐cause dementia diagnosis but not incidence of cognitive decline on neuropsychological examinations. In subgroup analysis, the effect was more prominent when using BPV of shorter timeframes, during shorter follow‐ups, or among the elderly aged more than 65 years. No dose‐response relationship could be found. Our study suggested possible positive associations between SBPV and the risk of dementia. Further studies are required to validate these findings. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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19. Nocturnal thoracic volume overload and post‐discharge outcomes in patients hospitalized for acute heart failure.
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Chang, Hao‐Chih, Huang, Chi‐Jung, Cheng, Hao‐Min, Yu, Wen‐Chung, Chiang, Chern‐En, Sung, Shih‐Hsien, and Chen, Chen‐Huan
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HEART failure ,LEFT heart ventricle - Abstract
Aims: Volume overload and perturbations of pulsatile haemodynamics may precipitate acute heart failure (AHF). Nocturnal thoracic volume overload due to rostral fluid shift during recumbency undetected by daytime measures may impact nighttime haemodynamics and post‐discharge outcomes. Methods and results: A total of 63 patients (median 60 years, 79.4% men, and left ventricular ejection fraction 29.4%) hospitalized for AHF were enrolled. Once clinical euvolaemia was achieved, noninvasive pulsatile haemodynamics were assessed during daytime followed by circadian monitoring (6 p.m. to 5 a.m.) of thoracic fluid content and thoracic fluid content index (TFCi) using impedance cardiography, normalized electromechanical activation time ratio (EMAT%) using acoustic cardiography, and mean blood pressure using ambulatory blood pressure monitoring before discharge. The primary endpoints were composited of the first hospitalization for heart failure and death from any cause. Patients were also followed for the repeated heart failure hospitalizations. During a median follow‐up duration of 16 months, 33 patients encountered primary composite endpoints (52.4%), and there were 42 hospitalizations developed among 25 patients. An overnight increase in TFCi along with persistently prolonged EMAT% and low mean blood pressure was observed in the eventful group. Overnight increase in TFCi (ΔTFCi, the difference between the measures at 4 a.m. and 6 p.m.) was an independent predictor of primary composite events (hazard ratio and 95% confidence interval: 1.58, 1.07–2.33; P = 0.022) and recurrent composite events (2.22, 1.51–3.26; P < 0.001), after adjusting for potential confounding factors. A high ΔTFCi (≥0.5/kΩ/m2) significantly correlated with higher post‐discharge events (hazard ratio 6.25; 95% confidence interval 2.30–16.96; P < 0.001) in comparison with a low ΔTFCi (<0.5/kΩ/m2). ΔTFCi was significantly associated with EMAT%, estimated glomerular filtration rate, and left ventricular ejection fraction, but not with parameters of pulsatile haemodynamics. Conclusions: Nocturnal thoracic volume overload in AHF before discharge, indicating the presence of residual volume overload unidentified by daytime measures, may predict post‐discharge outcomes. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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20. The role of pulmonary function in patients with heart failure and preserved ejection fraction: Looking beyond chronic obstructive pulmonary disease.
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Huang, Wei-Ming, Feng, Jia-Yih, Cheng, Hao-Min, Chen, Su-Zhen, Huang, Chi-Jung, Guo, Chao-Yu, Yu, Wen-Chung, Chen, Chen-Huan, and Sung, Shih-Hsien
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OBSTRUCTIVE lung diseases ,HEART failure patients ,COMORBIDITY ,SYSTOLIC blood pressure ,EXPIRATORY flow ,PULMONARY function tests ,INHALERS - Abstract
Background: The prognostic value of chronic obstructive pulmonary disease (COPD) as a comorbidity in heart failure has been well documented. However, the role of pulmonary function indices in patients with heart failure and preserved ejection fraction (HFpEF) remains to be elucidated. Methods: Subjects with HFpEF received pulmonary function tests and echocardiogram. Total lung capacity (TLC), residual volume (RV), forced expiratory flow rate between 25% and 75% of vital capacity (FEF25-75), forced expiratory volume in the 1
st second (FEV1), forced vital capacity (FVC), and vital capacity (VC) were measured. Echocardiographic indices, including pulmonary artery systolic pressure (PASP), the ratio of early ventricular filling flow velocity to the septal mitral annulus tissue velocity (E/e'), and left ventricular mass (LVM), were recorded. National Death Registry was linked for the identification of mortality. Results: A total of 1194 patients (72.4±13.2 years, 59% men) were enrolled. PASP, E/e' and LVM were associated with either obstructive (RV/TLC, FEV1 and FEF25-75) or restrictive (VC and TLC) ventilatory indices. During a mean follow-up of 23.0±12.8 months, 182 patients died. Subjects with COPD had a lower survival rate than those without COPD. While VC, FVC, RV/TLC, and FEV1 were all independently associated with all-cause mortality in patients without COPD, only FEF25-75 was predictive of outcomes in those with COPD. Conclusions: The abnormalities of pulmonary function were related to the cardiac hemodynamics in patients with HFpEF. In addition, these ventilatory indices were independently associated with long-term mortality, especially in those without COPD. [ABSTRACT FROM AUTHOR]- Published
- 2020
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21. Perturbations of pulsatile hemodynamics and clinical outcomes in patients with acute heart failure and reduced, mid-range or preserved ejection fraction.
- Author
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Huang, Wei-Ming, Sung, Shih-Hsien, Yu, Wen-Chung, Cheng, Hao-Min, Huang, Chi-Jung, Guo, Chao-Yu, Lu, Dai-Yin, Lee, Ching-Wei, and Chen, Chen-Huan
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HEART failure patients ,HEMODYNAMICS ,HEART failure - Abstract
Background: Heart failure with mid-range ejection fraction (HFmrEF) has been proposed as a new phenotype of heart failure. We therefore investigated the pulsatile hemodynamic characteristics and outcomes in patients with HFmrEF, in comparison with those with reduced (HFrEF) or preserved (HFpEF) ejection fraction. Methods: The study was composed of two cohorts of patients hospitalized due to acute heart failure. Pulsatile hemodynamic measures, including carotid-femoral pulse wave velocity (cf-PWV), carotid pulse pressure (cPP), amplitude of the backward pressure wave (Pb) and carotid augmentation index (cAIx), were recorded on admission and before discharge in Cohort A (n = 230, mean age 69.9 ±15.4 years), and long-term follow-up was performed in Cohort B (n = 2677, mean age 76.3 ± 33.4 years). Results: In Cohort A, patients with HFmrEF had persistently greater cf-PWV, cPP, Pb, and cAI than those with HFrEF, both on admission and before discharge. In contrast, patients with HFmrEF and HFpEF had similar pulsatile hemodynamic characteristics. In cohort B, patients with HFmrEF and HFrEF had similar three-year mortality rates and both were significantly higher than that in patients with HFpEF (both P values < 0.05). Conclusions: Patients with HFmrEF were characterized by a worse left ventricular systolic function than patients with HFpEF and excessive wave reflections than patients with HFrEF. Future studies are required to confirm that the unfavorable ventriculo-arterial coupling in HFmrEF might play a role in the pathogenesis of high long-term mortality in these patients. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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22. Dynamic expression of SMAD3 is critical in osteoblast differentiation of PDMCs.
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Lin, Hsi-Ting, Chen, Shao-Kuan, Guo, Jiun-Wen, Su, I-Chang, Huang, Chi-Jung, Chien, Chih-Cheng, and Chang, Chih-Ju
- Published
- 2019
23. Clinical significance of interleukin-6 and inducible nitric oxide synthase in ketamine-induced cystitis.
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Huang, Chi-Jung, Lee, Fa-Kung, Chen, Shao-Kuan, Chien, Chih-Cheng, Wu, Sheng-Tang, and Wang, Yen-Chieh
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- 2018
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24. Performance of AHEAD Score in an Asian Cohort of Acute Heart Failure With Either Preserved or Reduced Left Ventricular Systolic Function.
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Chen, Yu ‐ Jen, Sung, Shih ‐ Hsien, Cheng, Hao ‐ Min, Huang, Wei ‐ Ming, Wu, Chung ‐ Li, Huang, Chi ‐ Jung, Hsu, Pai ‐ Feng, Yeh, Jong ‐ Shiuan, Guo, Chao ‐ Yu, Yu, Wen ‐ Chung, and Chen, Chen ‐ Huan
- Published
- 2017
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25. Potential prognostic and predictive value of UBE2N, IMPDH1, DYNC1LI1 and HRASLS2 in colorectal cancer stool specimens.
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Chen, Yu-Nung, Shih, Cheng-Yen, Guo, Shu-Lin, Liu, Chih-Yi, Shen, Ming-Hung, Chang, Shih-Chang, Ku, Wei-Chi, Huang, Chi-Cheng, and Huang, Chi-Jung
- Subjects
COLORECTAL cancer ,PROGNOSIS ,FECAL occult blood tests ,UBIQUITIN-conjugating enzymes ,INOSINE monophosphate - Abstract
Colorectal cancer (CRC) is the most common gastrointestinal malignancy worldwide. The poor specificity and sensitivity of the fecal occult blood test has prompted the development of CRC-related genetic markers for CRC screening and treatment. Gene expression profiles in stool specimens are effective, sensitive and clinically applicable. Herein, a novel advantage of using cells shed from the colon is presented for cost-effective CRC screening. Molecular panels were generated through a series of leave-one-out cross-validation and discriminant analyses. A logistic regression model following reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry was used to validate a specific panel for CRC prediction. The panel, consisting of ubiquitin-conjugating enzyme E2 N (UBE2N), inosine monophosphate dehydrogenase 1 (IMPDH1), dynein cytoplasmic 1 light intermediate chain 1 (DYNC1LI1) and phospholipase A and acyltransferase 2 (HRASLS2), accurately recognized patients with CRC and could thus be further investigated as a potential prognostic and predictive biomarker for CRC. UBE2N, IMPDH1 and DYNC1LI1 expression levels were upregulated and HRASLS2 expression was downregulated in CRC tissues. The predictive power of the panel was 96.6% [95% confidence interval (CI), 88.1-99.6%] sensitivity and 89.7% (95% CI, 72.6-97.8%) specificity at a predicted cut-off value at 0.540, suggesting that this four-gene panel testing of stool specimens can faithfully mirror the state of the colon. On the whole, the present study demonstrates that screening for CRC or cancer detection in stool specimens collected non-invasively does not require the inclusion of an excessive number of genes, and colonic defects can be identified via the detection of an aberrant protein in the mucosa or submucosa. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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26. Upregulation of the growth arrest-specific-2 in recurrent colorectal cancers, and its susceptibility to chemotherapy in a model cell system.
- Author
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Huang, Chi-Jung, Lee, Chia-Long, Yang, Shung-Haur, Chien, Chih-Cheng, Huang, Chi-Cheng, Yang, Ruey-Neng, and Chang, Chun-Chao
- Subjects
- *
COLON cancer treatment , *CANCER relapse , *DISEASE susceptibility , *CANCER chemotherapy , *GENE expression , *CANCER cell proliferation - Abstract
Colorectal cancer (CRC) is one of the most common life-threatening malignances worldwide. CRC relapse markedly decreases the 5-year survival of patients following surgery. Aberrant expression of genes involved in pathways regulating the cell cycle, cell proliferation, or cell death are frequently reported in CRC tumorigenesis. We hypothesized that genes involved in CRC relapse might serve as prognostic indicators. We first evaluated the significance of gene sequences in the feces of patients with CRC relapse by consulting a public database. Tumorigenesis of target tissues was tested through tumor cell growth, cell cycle regulation, and chemotherapeutic efficacy. We found a highly significant correlation between CRC relapse and growth arrest-specific 2 (GAS2) gene expression. Based on cell models, the overexpressed GAS2 was associated with cellular growth rate, cell cycle regulation, and with chemotherapeutic sensitivity. Cell division was impaired by treating cells with 2-[4-(7-chloro-2-quinoxalinyloxy)phenoxy]-propionic acid (XK469), even when the cells were overexpressing GAS2. Thus, downregulation of GAS2 expression might control CRC relapse after curative resection. GAS2 could serve as a noninvasive marker from the feces of patients with prediagnosed CRC. Our findings suggest that GAS2 could have potential clinical applications for predicting early CRC relapse after radical resection, and that XK469 might impair tumor cell division by reducing GAS2 expression or blocking its cellular translocation. This will help in selecting the best therapeutic option, 5-fluorouracil in combination with XK469, for patients overexpressing GAS2 in CRC cells. Thus, GAS2 might act as a prognostic biomolecule and potential therapeutic target in patients with CRC relapse. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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27. Demethylation of HIN-1 reverses paclitaxel-resistance of ovarian clear cell carcinoma through the AKT-mTOR signaling pathway.
- Author
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Chih-Ming Ho, Chi-Jung Huang, Shih-Hung Huang, Shwu-Fen Chang, Wen-Fang Cheng, Ho, Chih-Ming, Huang, Chi-Jung, Huang, Shih-Hung, Chang, Shwu-Fen, and Cheng, Wen-Fang
- Subjects
OVARIAN cancer treatment ,OVARIAN cancer patients ,PACLITAXEL ,DRUG resistance in cancer cells ,HYPOXIA-inducible factor 1 ,DEMETHYLATION ,PROTEIN kinase B ,HEALTH outcome assessment ,ADENOCARCINOMA ,ANIMAL experimentation ,ANTINEOPLASTIC agents ,CELL lines ,CELLULAR signal transduction ,CYTOKINES ,MICE ,OVARIAN tumors ,PROTEINS ,TRANSFERASES ,DNA methylation ,PHARMACODYNAMICS - Abstract
Background: Methylation of HIN-1 is associated with poor outcomes in patients with ovarian clear cell carcinoma (OCCC), which is regarded to be an aggressive, chemo-resistant histological subtype. This study aimed to evaluate whether 5-aza-2-deoxycytidine (5-aza-2-dC) can reverse methylation of the HIN-1 gene to restore chemo-sensitivity of OCCC and the possible mechanism.Methods: In vitro flow cytometric analysis and evaluation of caspase-3/7 activity of paclitaxel-sensitive and resistant OCCC cell lines were performed. Methylation status and expression changes of HIN-1 in the OCCC cell lines treated with 5-aza-2-dC were evaluated, and immunohistochemical staining of HIN-1 in OCCC tissues was performed. In vivo tumor growth with or without 5-aza-2-dC treatment was analyzed, and Western blotting of AKT-mTOR signaling-related molecules was performed.Results: G2-M phase arrest was absent in paclitaxel-resistant OCCC cells after treatment with the cytotoxic drug. The caspase activities of the chemo-resistant OCCC cells were lower than those of the chemo-sensitive OCCC cells when treated with paclitaxel. Methylation of HIN-1 was noted in paclitaxel-resistant OCCC cell lines and cancerous tissues. 5-aza-2-dC reversed the methylation of HIN-1, re-activated the expression of HIN-1, and then suppressed the in vivo tumor growth of paclitaxel-resistant OCCC cells. Immunoblotting revealed that phospho-AKT473 and phospho-mTOR were significantly increased in HIN-1-methylated paclitaxel-resistant OCCC cell lines. However, the expressions of phospho-AKT at Ser473 and Thr308 and phospho-mTOR decreased in the OCCC cells with a high expression of HIN-1.Conclusions: Demethylating agents can restore the HIN-1 expression in paclitaxel-resistant OCCC cells through the HIN-1-AKT-mTOR signaling pathway to inhibit tumor growth. [ABSTRACT FROM AUTHOR]- Published
- 2015
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28. Deciphering Genetic Alterations of Taiwanese Patients with Pancreatic Adenocarcinoma through Targeted Sequencing.
- Author
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Huang, Chi-Cheng, Liu, Chih-Yi, Huang, Chi-Jung, Hsu, Yao-Chun, Lien, Heng-Hui, Wong, Jia-Uei, Tai, Feng-Chuan, Ku, Wen-Hui, Hung, Chi-Feng, Lin, Jaw-Town, Huang, Ching-Shui, and Chiang, Han-Sun
- Subjects
SOMATIC mutation ,PROTEIN-tyrosine kinase inhibitors ,TAIWANESE people ,NUCLEOTIDE sequencing ,ADENOCARCINOMA - Abstract
Pancreatic adenocarcinoma (PAC) is the 8th leading cause of cancer-related deaths in Taiwan, and its incidence is increasing. The development of PAC involves successive accumulation of multiple genetic alterations. Understanding the molecular pathogenesis and heterogeneity of PAC may facilitate personalized treatment for PAC and identify therapeutic agents. We performed tumor-only next-generation sequencing (NGS) with targeted panels to explore the molecular changes underlying PAC patients in Taiwan. The Ion Torrent Oncomine Comprehensive Panel (OCP) was used for PAC metastatic lesions, and more PAC samples were sequenced with the Ion AmpliSeq Cancer Hot Spot (CHP) v2 panel. Five formalin-fixed paraffin-embedded (FFPE) metastatic PAC specimens were successfully assayed with OCP, and KRAS was the most prevalent alteration, which might contraindicate the use of anti-EGFR therapy. One PAC patient harbored a FGFR2 p. C382R mutation, which might benefit from FGFR tyrosine kinase inhibitors. An additional 38 samples assayed with CHP v2 showed 100 hotspot variants, collapsing to 54 COSMID IDs. The most frequently mutated genes were TP53, KRAS, and PDGFRA (29, 23, 10 hotspot variants), impacting 11, 23, and 10 PAC patients. Highly pathogenic variants, including COSM22413 (PDGFRA, FATHMM predicted score: 0.88), COSM520, COSM521, and COSM518 (KRAS, FATHMM predicted score: 0.98), were reported. By using NGS with targeted panels, somatic mutations with therapeutic potential were identified. The combination of clinical and genetic information is useful for decision making and precise selection of targeted medicine. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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29. The Periodontopathic Pathogen, Porphyromonas gingivalis , Involves a Gut Inflammatory Response and Exacerbates Inflammatory Bowel Disease.
- Author
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Lee, Yu-Chen, Liu, Chih-Yi, Lee, Chia-Long, Zhang, Ruo-Han, Huang, Chi-Jung, and Yen, Ting-Lin
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INFLAMMATORY bowel diseases ,PORPHYROMONAS gingivalis ,INFLAMMATION ,CROHN'S disease ,VIRUS diseases ,PATHOGENIC microorganisms - Abstract
Periodontal disease (PD) is one of the most prevalent disorders globally and is strongly associated with many other diseases. Inflammatory bowel disease (IBD), an inflammatory condition of the colon and the small intestine, is reported to be associated with PD through undetermined mechanisms. We analyzed taxonomic assignment files from the Crohn's Disease Viral and Microbial Metagenome Project (PRJEB3206). The abundance of Porphyromonadaceae in fecal samples was significantly different between patients with Crohn's disease and control volunteers. Dextran sulfate sodium was used to induce colitis in mice to reveal the effect of this periodontopathic pathogen in vivo. After intrarectal implantation of Porphyromonas gingivalis (Pg)—the primary pathogen causing PD—the disease activity index score, colonic epithelial loss, and inflammatory cell infiltration were intensified. In addition, tumor necrosis factor-α and interleukin-6 showed the highest levels in Pg-infected colons. This revealed the importance of Pg in the exacerbation of IBD. Thus, simultaneous treatment of PD should be considered for people with IBD. Moreover, implantation of Pg in the rectum worsened the clinical symptoms of colitis in mice. Because Pg participates in the pathogenesis of IBD, reducing the chances of it entering the intestine might prevent the worsening of this disorder. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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30. Supplementation of Probiotic Butyricicoccus pullicaecorum Mediates Anticancer Effect on Bladder Urothelial Cells by Regulating Butyrate-Responsive Molecular Signatures.
- Author
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Wang, Yen-Chieh, Ku, Wei-Chi, Liu, Chih-Yi, Cheng, Yu-Che, Chien, Chih-Cheng, Chang, Kang-Wei, and Huang, Chi-Jung
- Subjects
BLADDER cancer ,ANTINEOPLASTIC agents ,BLADDER ,SHORT-chain fatty acids ,TRANSITIONAL cell carcinoma ,PROBIOTICS - Abstract
In bladder cancer, urothelial carcinoma is the most common histologic subtype, accounting for more than 90% of cases. Pathogenic effects due to the dysbiosis of gut microbiota are localized not only in the colon, but also in regulating bladder cancer distally. Butyrate, a short-chain fatty acid produced by gut microbial metabolism, is mainly studied in colon diseases. Therefore, the resolution of the anti-cancer effects of butyrate-producing microbes on bladder urothelial cells and knowledge of the butyrate-responsive molecules must have clinical significance. Here, we demonstrate a correlation between urothelial cancer of the bladder and Butyricicoccus pullicaecorum. This butyrate-producing microbe or their metabolite, butyrate, mediated anti-cancer effects on bladder urothelial cells by regulating cell cycle, cell growth, apoptosis, and gene expression. For example, a tumor suppressor against urothelial cancer of the bladder, bladder cancer-associated protein, was induced in butyrate-treated HT1376 cells, a human urinary bladder cancer cell line. In conclusion, urothelial cancer of the bladder is a significant health problem. To improve the health of bladder urothelial cells, supplementation of B. pullicaecorum may be necessary and can further regulate butyrate-responsive molecular signatures. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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31. Co-Occurrence of Differentiated Thyroid Cancer and Second Primary Malignancy: Correlation with Expression Profiles of Mismatch Repair Protein and Cell Cycle Regulators.
- Author
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Liu, Chih-Yi, Huang, Ching-Shui, Huang, Chi-Cheng, Ku, Wei-Chi, Shih, Hsing-Yu, and Huang, Chi-Jung
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PROTEINS ,CONFIDENCE intervals ,THYROID gland tumors ,MULTIVARIATE analysis ,SURGERY ,PATIENTS ,DISEASE relapse ,CELL cycle ,CANCER patients ,SECONDARY primary cancer ,GENE expression profiling ,DESCRIPTIVE statistics ,STATISTICAL sampling ,ODDS ratio - Abstract
Simple Summary: Although the incidence of thyroid cancer is increasing, improvements in treatment have resulted in more patients being confirmed to have a second primary cancer. However, studies on potential biomarkers for predicting the risk of second primary malignancy are extremely limited. Therefore, our objective was to establish molecular biomarkers for the risk prediction of second primary malignancy using routinely collected formalin-fixed paraffin-embedded tissue specimens. Our results suggest that the deficient mismatch repair phenotype, the expression of pRb, and the lack of CDK4 or CDK6 are significantly associated with co-occurrence of nonthyroid malignancy. The predictive value of these immunohistochemical profiles for the co-occurrence of nonthyroid malignancy was also assessed. The combined evaluation of a four-biomarker signature model may provide the most important predictive innovation. Our study proposes the first tissue-based screening tool for risk stratification and further active surveillance in patients with thyroid cancer. Some patients with thyroid cancer develop a second primary cancer. Defining the characteristics of patients with double primary cancers (DPCs) is crucial and needs to be followed. In this study, we examine molecular profiles in DPC. We enrolled 71 patients who received thyroid cancer surgery, 26 with single thyroid cancer (STC), and 45 with DPC. A retrograde cohort was used to develop immunohistochemical expressions of mismatch repair (MMR) proteins and cell-cycle-related markers from tissue microarrays to produce an equation for predicting the occurrence of DPC. The multivariate logistic model of 67 randomly selected patients (24 with STC and 43 with DPC) identified that the expression of deficient MMR (dMMR) (odds ratio (OR), 10.34; 95% confidence interval (CI), 2.17–49.21) and pRb (OR, 62.71; 95% CI, 4.83–814.22) were significantly associated with a higher risk of DPC. In contrast, the expression of CDK4 (OR, 0.19; 95% CI, 0.04–0.99) and CDK6 (OR, 0.03; 95% CI, 0.002–0.44) was significantly associated with a lower risk of DPC. Collectively, dMMR, pRb, CDK4, and CDK6 have a sensitivity of 88.9% (95% CI, 75.1–95.8) and a specificity of 69.2% (95% CI, 48.1–84.9) for occurrence of DPC in all 71 patients. This is the first report to demonstrate the molecular differentiation of STC and DPC. Overall, the integral molecular profile performed excellent discrimination and denoted an exponential function to predict the probability of DPC. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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32. Targeted Sequencing of Taiwanese Breast Cancer with Risk Stratification by the Concurrent Genes Signature: A Feasibility Study.
- Author
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Huang, Ching-Shui, Liu, Chih-Yi, Lu, Tzu-Pin, Huang, Chi-Jung, Chiu, Jen-Hwey, Tseng, Ling-Ming, and Huang, Chi-Cheng
- Subjects
DISEASE risk factors ,GENETIC mutation ,NOTCH genes ,BREAST cancer ,SOMATIC mutation - Abstract
Breast cancer is the most common female malignancy in Taiwan, while conventional clinical and pathological factors fail to provide full explanation for prognostic heterogeneity. The aim of the study was to evaluate the feasibility of targeted sequencing combined with concurrent genes signature to identify somatic mutations with clinical significance. The extended concurrent genes signature was based on the coherent patterns between genomic and transcriptional alterations. Targeted sequencing of 61 Taiwanese breast cancers revealed 1036 variants, including 76 pathogenic and 545 likely pathogenic variants based on the ACMG classification. The most frequently mutated genes were NOTCH, BRCA1, AR, ERBB2, FANCA, ATM, and BRCA2 and the most common pathogenic deletions were FGFR1, ATM, and WT1, while BRCA1 (rs1799965), FGFR2 (missense), and BRCA1 (rs1799949) were recurrent pathogenic SNPs. In addition, 38 breast cancers were predicted into 12 high-risk and 26 low-risk cases based on the extended concurrent genes signature, while the pathogenic PIK3CA variant (rs121913279) was significantly mutated between groups. Two deleterious SH3GLB2 mutations were further revealed by multivariate Cox's regression (hazard ratios: 29.4 and 16.1). In addition, we identified several significantly mutated or pathogenic variants associated with differentially expressed signature genes. The feasibility of targeted sequencing in combination with concurrent genes risk stratification was ascertained. Future study to validate clinical applicability and evaluate potential actionability for Taiwanese breast cancers should be initiated. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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33. A gut butyrate-producing bacterium Butyricicoccus pullicaecorum regulates short-chain fatty acid transporter and receptor to reduce the progression of 1,2-dimethylhydrazine-associated colorectal cancer.
- Author
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Chang, Shih-Chang, Shen, Ming-Hung, Liu, Chih-Yi, Pu, Chi-Ming, Hu, Je-Ming, and Huang, Chi-Jung
- Subjects
SHORT-chain fatty acids ,COLORECTAL cancer ,CARCINOEMBRYONIC antigen ,PAPILLOMAVIRUSES ,IMMUNOSTAINING ,COLON (Anatomy) ,BACTEROIDES fragilis - Abstract
Gut microbes influence tumor development and progression in the intestines and may provide a novel paradigm for the treatment of colorectal cancer (CRC). Gut dysbiosis may be associated with the development and progression of CRC. Identifying the interactions between the colonic tract and gut microbiota may provide novel information relevant to CRC prevention. The present study examined the effects of butyrate-producing Butyricicoccus pullicaecorum (B. pullicaecorum) on mice with 1,2-dimethylhydrazine (DMH)-induced CRC and the microbial metabolite of B. pullicaecorum on CRC cells. Immunohistochemical staining of the mouse colon tissues and reverse transcription PCR of CRC cells were used to determine the protein and mRNA expression levels of the short-chain fatty acid (SCFA) transporter solute carrier family 5 member 8 (SLC5A8) and G-protein-coupled receptor 43 (GPR43). In CRC-bearing mice fed B. pullicaecorum, DMH-induced CRC regressed, body weight increased and serum carcinoembryonic antigen levels decreased. Notably, SLC5A8 and GPR43 were diffusely and moderately to strongly expressed in the neoplastic epithelial cells and underlying muscularis propria in the colons of the mice. In conclusion, administration of B. pullicaecorum or its metabolites improved the clinical outcome of CRC by activating the SCFA transporter and/or receptor. These results indicated that B. pullicaecorum was a probiotic with anti-CRC potential. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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34. Co-Expression of Coxsackievirus/Adenovirus Receptors and Desmoglein 2 in Lung Adenocarcinoma: A Comprehensive Analysis of Bioinformatics and Tissue Microarrays.
- Author
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Weng, Ching-Fu, Huang, Chi-Jung, Wu, Mei-Hsuan, Lee, Henry Hsin-Chung, and Ling, Thai-Yen
- Subjects
- *
PROGNOSIS , *EPITHELIAL-mesenchymal transition , *TISSUE analysis , *ADENOVIRUSES , *SURVIVAL analysis (Biometry) - Abstract
Introduction: Coxsackievirus/adenovirus receptors (CARs) and desmoglein-2 (DSG2) are similar molecules to adenovirus-based vectors in the cell membrane. They have been found to be associated with lung epithelial cell tumorigenesis and can be useful markers in predicting survival outcome in lung adenocarcinoma (LUAD). Methods: A gene ontology enrichment analysis disclosed that DSG2 was highly correlated with CAR. Survival analysis was then performed on 262 samples from the Cancer Genome Atlas, forming "Stage 1A" or "Stage 1B". We therefore analyzed a tissue microarray (TMA) comprised of 108 lung samples and an immunohistochemical assay. Computer counting software was used to calculate the H-score of the immune intensity. Cox regression and Kaplan–Meier analyses were used to determine the prognostic value. Results: CAR and DSG2 genes are highly co-expressed in early stage LUAD and associated with significantly poorer survival (p = 0.0046). TMA also showed that CAR/DSG2 expressions were altered in lung cancer tissue. CAR in the TMA was correlated with proliferation, apoptosis, and epithelial–mesenchymal transition (EMT), while DSG2 was associated with proliferation only. The Kaplan–Meier survival analysis revealed that CAR, DSG2, or a co-expression of CAR/DSG2 was associated with poorer overall survival. Conclusions: The co-expression of CAR/DSG2 predicted a worse overall survival in LUAD. CAR combined with DSG2 expression can predict prognosis. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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35. New International Association for the Study of Lung Cancer (IASLC) Pathology Committee Grading System for the Prognostic Outcome of Advanced Lung Adenocarcinoma.
- Author
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Weng, Ching-Fu, Huang, Chi-Jung, Huang, Shih-Hung, Wu, Mei-Hsuan, Tseng, Ailun Heather, Sung, Yung-Chuan, Lee, Henry Hsin-Chung, and Ling, Thai-Yen
- Subjects
- *
LUNG cancer prognosis , *ADENOCARCINOMA , *BIOPSY , *CANCER patients , *LUNG cancer , *MULTIVARIATE analysis , *GENETIC mutation , *REGRESSION analysis , *SMOKING , *STATISTICS , *PROTEIN-tyrosine kinase inhibitors , *BODY mass index , *EPIDERMAL growth factor receptors , *KAPLAN-Meier estimator - Abstract
Simple Summary: This study investigated the association between survival outcome and the new grading system among advanced stage lung adenocarcinoma (LADC) (stages IIIA, IIIB and IV) patients who were diagnosed as LADC with a pathologic report according to a new grading system by the International Association for the Study of Lung Cancer (IASLC) pathology committee. The results indicate that the poorly differentiated group had a poorer prognosis in PFS, as did patients with wild-type EGFR who were treated with chemotherapy. No survival difference could be found among EGFR mutation patients. Older age and a lower body mass index also led to worse survival. Patients with poorly differentiated adenocarcinoma likewise had worse survival, especially compared to those with moderately differentiated adenocarcinoma. Our findings highlight that the therapeutic regimen should be adjusted for wild-type EGFR patients with poorly differentiated adenocarcinoma treated with chemotherapy to provide better outcomes. No survival difference could be seen among EGFR mutation patients. The impact of the new International Association for the Study of Lung Cancer pathology committee grading system for advanced lung adenocarcinoma (LADC) on survival is unclear, especially in Asian populations. In this study, we reviewed the prognostic outcomes of patients with late-stage disease according to the new grading system. We reviewed 136 LADC cases who underwent a small biopsy from 2007 to 2018. Tumors were classified according to the new grading system for LADC. Baseline characteristics (age, sex, smoking status, body mass index, and driver gene mutations) were analyzed. Kaplan–Meier and Cox regression analyses were used to determine correlations with the new grading system and prognosis. Patients with poorly differentiated adenocarcinoma were significantly correlated with a poor progression-free survival (PFS) (p = 0.013) but not overall survival (OS) (p = 0.154). Subgroup analysis showed that wild-type EGFR patients with poorly differentiated adenocarcinoma treated with chemotherapy had significantly worse PFS (p = 0.011). There was no significant difference in survival among the patients with epidermal growth factor receptor mutations who were treated with tyrosine kinase inhibitors. Patients aged >70 years and those with a BMI ≤ 25 kg/m2 and wild-type patients had significantly worse OS in both univariate (HR = 1.822, p = 0.006; HR = 2.250, p = 0.004; HR = 1.537, p = 0.046, respectively) and multivariate analyses (HR = 1.984, p = 0.002; HR = 2.383, p = 0.002; HR = 1.632, p = 0.028, respectively). Despite therapy, patients with poorly differentiated tumors still fared worse than those with better differentiated tumors. No differences were found among the EGFR mutations treated with TKI. Our findings highlight that the therapeutic regimen should be adjusted for EGFR Wild-type patients with poorly differentiated adenocarcinoma treated with chemotherapy to provide better outcomes. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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- View/download PDF
36. Salvianolic Acid B in Microemulsion Formulation Provided Sufficient Hydration for Dry Skin and Ameliorated the Severity of Imiquimod-induced Psoriasis-like Dermatitis in Mice.
- Author
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Guo, Jiun-Wen, Cheng, Yu-Pin, Liu, Chih-Yi, Thong, Haw-Yueh, Huang, Chi-Jung, Lo, Yang, Wu, Chen-Yu, and Jee, Shiou-Hwa
- Subjects
MICROEMULSIONS ,TREATMENT effectiveness ,HYDRATION ,SKIN inflammation ,PATHOLOGY ,SKIN ,IMIQUIMOD - Abstract
Psoriasis is a chronic inflammatory skin disorder with a pathogenesis involving the interleukin-23/interleukin-17 axis. Salvianolic acid B exerts several pharmacological effects, such as antioxidation, anti-inflammation, and antitumor effects. The anti-psoriatic effects of salvianolic acid B have not been reported. In this study, we aimed to determine the optimum vehicle for salvianolic acid B, investigate its therapeutic effect on psoriatic-like skin conditions, and explore its underlying mechanisms of action. BALB/c mice were administered topical imiquimod to induce psoriasis-like skin and were then randomly assigned to control, vehicle control, salvianolic acid B in vehicles, and 0.25% desoximetasone ointment treatment groups. Barrier function, cytokine expression, histology assessment, and disease severity were evaluated. The results showed that salvianolic acid B-containing microemulsion alleviated disease severity, reduced acanthosis, and inhibited interleukin-23/interleukin-17 (IL-23/IL-17) cytokines, epidermal proliferation, and increased skin hydration. Our study suggests that salvianolic acid B represents a possible new therapeutic drug for the treatment of psoriasis. In addition, such formulation could obtain high therapeutic efficacy in addition to providing sufficient hydration for dry skin. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
37. Does statin increase the risk of intracerebral hemorrhage in stroke survivors? A meta-analysis and trial sequential analysis.
- Author
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Teoh, Ru Jian Jonathan, Huang, Chi-Jung, Chan, Chi Peng, Chien, Li-Yin, Chung, Chih-Ping, Sung, Shih-Hsien, Chen, Chen-Huan, Chiang, Chern-En, and Cheng, Hao-Min
- Abstract
Background: It remains debatable whether statin increases the risk of intracerebral hemorrhage (ICH) in poststroke patients. Methods: We systematically searched PubMed, EMBASE, and CENTRAL for randomized controlled trials. Trial sequential analysis (TSA) was conducted to assess the reliability and conclusiveness of the available evidence in the meta-analysis. To evaluate the overall effectiveness, the net composite endpoints were derived by totaling ischemic stroke, hemorrhagic stroke, transient ischemic attack (TIA), myocardial infarction, and cardiovascular mortality. Results: A total of 17 trials with 11,576 subjects with previous ischemic stroke, TIA, or ICH were included, in which statin therapy increased the risk of hemorrhagic stroke (risk ratio [RR], 1.42; 95% confidence interval [CI], 1.07–1.87), but reduced the risk of ischemic stroke (RR, 0.85; 95% CI, 0.75–0.95). For the net composite endpoints, statin therapy was associated with a 17% risk reduction (95% CI, 12–21%; number needed to treat = 6). With a control event rate 2% and RR increase 40%, the TSA suggested a conclusive signal of an increased risk of hemorrhagic stroke in stroke survivors taking statin. However, with the sensitivity analysis by changing assumptions, the conclusions about hemorrhagic stroke risk were less robust. Conclusions: Statin therapy in poststroke patients increased the risk of hemorrhagic stroke but effectively reduced ischemic stroke risk. Weighing the benefits and potential harms, statin has an overall beneficial effect in patients with previous stroke or TIA. However, more studies are required to investigate the conclusiveness of the increased hemorrhagic stroke risk revealed in our study. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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- View/download PDF
38. Hemodialysis interval and its association with emergency care and mortality: A nationwide population-based cohort study.
- Author
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Chien, Ching-Wen, Huang, Chi-Jung, Chao, Zi-Hao, Huang, Song-Kong, Chen, Pei-En, Tung, Tao-Hsin, and Schaller., Bernhard
- Published
- 2019
- Full Text
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39. Serum CA125 concentration as a predictor of peritoneal dissemination of colorectal cancer in men and women.
- Author
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Huang, Chi-Jung, Jiang, Jeng-Kai, Chang, Shih-Ching, Lin, Jen-Kou, and Yang, Shung-Haur
- Published
- 2016
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- View/download PDF
40. Corrigendum: Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells.
- Author
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Cheng, Yu-Che, Huang, Chi-Jung, Lee, Yih-Jing, Tien, Lu-Tai, Ku, Wei-Chi, Chien, Raymond, Lee, Fa-Kung, and Chien, Chih-Cheng
- Published
- 2016
- Full Text
- View/download PDF
41. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells.
- Author
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Cheng, Yu-Che, Huang, Chi-Jung, Lee, Yih-Jing, Tien, Lu-Tai, Ku, Wei-Chi, Chien, Raymond, Lee, Fa-Kung, and Chien, Chih-Cheng
- Published
- 2016
- Full Text
- View/download PDF
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