17 results on '"Prado, Vania F."'
Search Results
2. Muscarinic and N‐methyl‐D‐aspartate receptor blockade reveal differences in hippocampal local field potentials in mice with low cholinergic tone.
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Leung, L. Stan, Moallem, Shahin, Prado, Marco A. M., Prado, Vania F., and Chu, Liangwei
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METHYL aspartate receptors ,MUSCARINIC receptors ,HIPPOCAMPUS (Brain) ,SCOPOLAMINE ,MUSCARINIC antagonists - Abstract
We hypothesize that hippocampal local field potentials in acetylcholine (ACh)‐deficient mutant mice, compared to wild‐type (WT) mice, will show lower sensitivity to muscarinic cholinergic antagonist scopolamine (5 mg/kg i.p.) but higher sensitivity to NMDA receptor antagonist 3‐(2‐carboxypiperazin‐4‐yl)propyl‐1‐phosphonic acid (CPP, 10 mg/kg i.p.). Recordings were made during walk and awake‐immobility (IMM) in WT mice, and in mice with forebrain knockout (KO) of the vesicular acetylcholine transporter (VAChT) gene, or heterozygous knockdown of VAChT gene (KD). Scopolamine or CPP did not significantly alter walk theta frequency, which was higher in KD than WT/KO mice. Scopolamine decreased theta power peak rise during walk in WT/KD mice but not in KO mice, while CPP suppressed theta peak rise more in WT/KO mice than KD mice. During IMM, scopolamine decreased gamma1 (γ1, 30–58 Hz) power more in KD/WT mice than KO mice, while delta (1–4 Hz) power and delta‐gamma cross‐frequency coherence (CFC) were increased in all mouse groups during IMM or walk. During walk, scopolamine increased delta and beta (13–30 Hz) power and decreased gamma2 (γ2, 62–100 Hz) power and theta‐γ2 CFC more in WT/KD than KO mice. Theta‐γ2, but not theta‐γ1, CFC increased with theta‐peak‐frequency in WT/KD mice, and was suppressed by scopolamine at high theta (8–10 Hz) frequency; theta‐γ2 CFC in KO mice was not significantly altered by scopolamine. CPP decreased beta and gamma power more in KD/KO mice compared to WT mice, while delta power and delta‐gamma CFC were increased in all mouse groups. ACh deficiency exacerbates the attenuation of beta and gamma power by CPP. We conclude that both muscarinic and NMDA transmission contribute toward hippocampal theta, beta, and gamma power, and a decrease in gamma power or theta‐gamma CFC may be associated with loss of arousal and cognitive functions. [ABSTRACT FROM AUTHOR]
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- 2022
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3. Functional dissociation of behavioral effects from acetylcholine and glutamate released from cholinergic striatal interneurons.
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Kljakic, Ornela, Janíčková, Helena, Skirzewski, Miguel, Reichelt, Amy, Memar, Sara, El Mestikawy, Salah, Li, Yulong, Saksida, Lisa M., Bussey, Timothy J., Prado, Vania F., and Prado, Marco A. M.
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- 2022
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4. Evaluating Sequential Response Learning in the Rodent Operant Touchscreen System.
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Janickova, Helena, Kljakic, Ornela, Robbins, Trevor W., Saksida, Lisa M., Bussey, Timothy J., Prado, Vania F., and Prado, Marco A. M.
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- 2021
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5. Cholinergic transmission from the basal forebrain modulates social memory in male mice.
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Kljakic, Ornela, Al‐Onaizi, Mohammed, Janíčková, Helena, Chen, Kevin S., Guzman, Monica S., Prado, Marco A. M., and Prado, Vania F.
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COLLECTIVE memory ,AUTISM spectrum disorders ,CHOLINERGIC mechanisms ,PROSENCEPHALON ,SOCIAL influence - Abstract
Disruptions in social behaviour are prevalent in many neuropsychiatric disorders such as schizophrenia, bipolar disorder and autism spectrum disorders. However, the underlying neurochemical regulation of social behaviour is still not well understood. The central cholinergic system has been proposed to contribute to the regulation of social behaviour. For instance, decreased global levels of acetylcholine release in the brain leads to decreased social interaction and an impairment of social memory in mice. Nonetheless, it has been difficult to ascertain the specific brain areas where cholinergic signalling influences social preference and social memory. In this study, we investigated the impact of different forebrain cholinergic regions on social behaviour by examining mouse lines that differ in their regional expression level of the vesicular acetylcholine transporter—the protein that regulates acetylcholine secretion. We found that when cholinergic signalling is highly disrupted in the striatum, hippocampus, cortex and amygdala mice have intact social preference but are impaired in social memory, as they cannot remember a familiar conspecific nor recognize a novel one. A similar pattern emerges when acetylcholine release is disrupted mainly in the striatum, cortex, and amygdala; however, the ability to recognize novel conspecifics is retained. In contrast, cholinergic signalling of the striatum and amygdala does not appear to significantly contribute to the modulation of social memory and social preference. Furthermore, we demonstrated that increasing global cholinergic tone does not increase social behaviours. Together, these data suggest that cholinergic transmission from the hippocampus and cortex are important for regulating social memory. [ABSTRACT FROM AUTHOR]
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- 2021
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6. Motoneuron‐specific loss of VAChT mimics neuromuscular defects seen in congenital myasthenic syndrome.
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Joviano‐Santos, Julliane V., Kljakic, Ornela, Magalhães‐Gomes, Matheus P. S., Valadão, Priscila Aparecida C., de Oliveira, Leonardo R., Prado, Marco A. M., Prado, Vania F., and Guatimosim, Cristina
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CONGENITAL myasthenic syndromes ,MOTOR neurons ,SPINAL cord ,HUMAN abnormalities ,LABORATORY mice ,SYNAPTIC vesicles - Abstract
Motoneurons (MNs) control muscle activity by releasing the neurotransmitter acetylcholine (ACh) at the level of neuromuscular junctions. ACh is packaged into synaptic vesicles by the vesicular ACh transporter (VAChT), and disruptions in its release can impair muscle contraction, as seen in congenital myasthenic syndromes (CMS). Recently, VAChT gene mutations were identified in humans displaying varying degrees of myasthenia. Moreover, mice with a global deficiency in VAChT expression display several characteristics of CMS. Despite these findings, little is known about how a long‐term decrease in VAChT expression in vivo affects MNs structure and function. Using Cre‐loxP technology, we generated a mouse model where VAChT is deleted in select groups of MNs (mnVAChT‐KD). Molecular analysis revealed that the VAChT deletion was specific to MNs and affected approximately 50% of its population in the brainstem and spinal cord, with alpha‐MNs primarily targeted (70% in spinal cord). Within each animal, the cell body area of VAChT‐deleted MNs was significantly smaller compared to MNs with VAChT preserved. Likewise, muscles innervated by VAChT‐deleted MNs showed atrophy while muscles innervated by VAChT‐containing neurons appeared normal. In addition, mnVAChT KD mice had decreased muscle strength, were hypoactive, leaner and exhibited kyphosis. This neuromuscular dysfunction was evident at 2 months of age and became progressively worse by 6 months. Treatment of mutants with a cholinesterase inhibitor was able to improve some of the motor deficits. As these observations mimic what is seen in CMS, this new line could be valuable for assessing the efficacy of potential CMS drugs. [ABSTRACT FROM AUTHOR]
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- 2021
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7. Hsp90 and its co‐chaperone Sti1 control TDP‐43 misfolding and toxicity.
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Lin, Lilian Tsai‐Wei, Razzaq, Abdul, Di Gregorio, Sonja E., Hong, Soojie, Charles, Brendan, Lopes, Marilene H., Beraldo, Flavio, Prado, Vania F., Prado, Marco A. M., and Duennwald, Martin L.
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- 2021
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8. New frontiers in translational research: Touchscreens, open science, and the mouse translational research accelerator platform.
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Sullivan, Jacqueline A., Dumont, Julie R., Memar, Sara, Skirzewski, Miguel, Wan, Jinxia, Mofrad, Maryam H., Ansari, Hassam Zafar, Li, Yulong, Muller, Lyle, Prado, Vania F., Prado, Marco A. M., Saksida, Lisa M., and Bussey, Timothy J.
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TRANSLATIONAL research ,TOUCH screens ,COGNITIVE testing ,DESIGN science ,COGNITIVE ability ,TEST systems - Abstract
Many neurodegenerative and neuropsychiatric diseases and other brain disorders are accompanied by impairments in high‐level cognitive functions including memory, attention, motivation, and decision‐making. Despite several decades of extensive research, neuroscience is little closer to discovering new treatments. Key impediments include the absence of validated and robust cognitive assessment tools for facilitating translation from animal models to humans. In this review, we describe a state‐of‐the‐art platform poised to overcome these impediments and improve the success of translational research, the Mouse Translational Research Accelerator Platform (MouseTRAP), which is centered on the touchscreen cognitive testing system for rodents. It integrates touchscreen‐based tests of high‐level cognitive assessment with state‐of‐the art neurotechnology to record and manipulate molecular and circuit level activity in vivo in animal models during human‐relevant cognitive performance. The platform also is integrated with two Open Science platforms designed to facilitate knowledge and data‐sharing practices within the rodent touchscreen community, touchscreencognition.org and mousebytes.ca. Touchscreencognition.org includes the Wall, showcasing touchscreen news and publications, the Forum, for community discussion, and Training, which includes courses, videos, SOPs, and symposia. To get started, interested researchers simply create user accounts. We describe the origins of the touchscreen testing system, the novel lines of research it has facilitated, and its increasingly widespread use in translational research, which is attributable in part to knowledge‐sharing efforts over the past decade. We then identify the unique features of MouseTRAP that stand to potentially revolutionize translational research, and describe new initiatives to partner with similar platforms such as McGill's M3 platform (m3platform.org). [ABSTRACT FROM AUTHOR]
- Published
- 2021
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9. Modulation of hippocampal neuronal resilience during aging by the Hsp70/Hsp90 co‐chaperone STI1.
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Lackie, Rachel E., Razzaq, Abdul R., Farhan, Sali M. K., Qiu, Lily R., Moshitzky, Gilli, Beraldo, Flavio H., Lopes, Marilene H., Maciejewski, Andrzej, Gros, Robert, Fan, Jue, Choy, Wing‐Yiu, Greenberg, David S., Martins, Vilma R., Duennwald, Martin L., Lerch, Jason P., Soreq, Hermona, Prado, Vania F., and Prado, Marco A. M.
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MICE ,SPATIAL memory ,HUMAN genes ,CELL lines ,CELL analysis ,NEURODEGENERATION - Abstract
Chaperone networks are dysregulated with aging, but whether compromised Hsp70/Hsp90 chaperone function disturbs neuronal resilience is unknown. Stress‐inducible phosphoprotein 1 (STI1; STIP1; HOP) is a co‐chaperone that simultaneously interacts with Hsp70 and Hsp90, but whose function in vivo remains poorly understood. We combined in‐depth analysis of chaperone genes in human datasets, analysis of a neuronal cell line lacking STI1 and of a mouse line with a hypomorphic Stip1 allele to investigate the requirement for STI1 in aging. Our experiments revealed that dysfunctional STI1 activity compromised Hsp70/Hsp90 chaperone network and neuronal resilience. The levels of a set of Hsp90 co‐chaperones and client proteins were selectively affected by reduced levels of STI1, suggesting that their stability depends on functional Hsp70/Hsp90 machinery. Analysis of human databases revealed a subset of co‐chaperones, including STI1, whose loss of function is incompatible with life in mammals, albeit they are not essential in yeast. Importantly, mice expressing a hypomorphic STI1 allele presented spontaneous age‐dependent hippocampal neurodegeneration and reduced hippocampal volume, with consequent spatial memory deficit. We suggest that impaired STI1 function compromises Hsp70/Hsp90 chaperone activity in mammals and can by itself cause age‐dependent hippocampal neurodegeneration in mice. Cover Image for this issue: doi: 10.1111/jnc.14749. [ABSTRACT FROM AUTHOR]
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- 2020
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10. Selective decrease of cholinergic signaling from pedunculopontine and laterodorsal tegmental nuclei has little impact on cognition but markedly increases susceptibility to stress.
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Janickova, Helena, Kljakic, Ornela, Rosborough, Kaie, Raulic, Sanda, Matovic, Sara, Gros, Robert, Saksida, Lisa M., Bussey, Timothy J., Inoue, Wataru, Prado, Vania F., and Prado, Marco A. M.
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- 2019
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11. Mechanisms of neuroprotection against ischemic insult by stress‐inducible phosphoprotein‐1/prion protein complex.
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Beraldo, Flavio H., Ostapchenko, Valeriy G., Xu, Jason Z., Di Guglielmo, Gianni M., Fan, Jue, Nicholls, Peter J., Caron, Marc G., Prado, Vania F., and Prado, Marco A. M.
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NEUROPROTECTIVE agents ,PHOSPHOPROTEINS ,CEREBRAL ischemia treatment ,PRIONS ,APOPTOSIS ,ACTIVIN receptors - Abstract
Abstract: Stress‐inducible phosphoprotein 1 (STI1) acts as a neuroprotective factor in the ischemic brain and its levels are increased following ischemia. Previous work has suggested that some of these STI1 actions in a stroke model depend on the recruitment of bone marrow‐derived stem cells to improve outcomes after ischemic insult. However, STI1 can directly increase neuroprotective signaling in neurons by engaging with the cellular prion protein (PrP
C ) and activating α7 nicotinic acetylcholine receptors (α7nAChR). Given that α7nAChR activation has also been involved in neuroprotection in stroke, it is possible that STI1 can have direct actions on neurons to prevent deleterious consequences of ischemic insults. Here, we tested this hypothesis by exposing primary neuronal cultures to 1‐h oxygen‐glucose deprivation (OGD) and reperfusion and assessing signaling pathways activated by STI1/PrPC . Our results demonstrated that STI1 treatment significantly decreased apoptosis and cell death in mouse neurons submitted to OGD in a manner that was dependent on PrPC and α7nAChR, but also on the activin A receptor 1 (ALK2), which has emerged as a signaling partner of STI1. Interestingly, pharmacological inhibition of the ALK2 receptor prevented neuroprotection by STI1, while activation of ALK2 receptors by bone morphogenetic protein 4 (BMP4) either before or after OGD was effective in decreasing neuronal death induced by ischemia. We conclude that PrPC /STI1 engagement and its subsequent downstream signaling cascades involving α7nAChR as well as the ALK2 receptor may be activated in neurons by increased levels of STI1. This signaling pathway protects neurons from ischemic insults. [ABSTRACT FROM AUTHOR]- Published
- 2018
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12. Vesicular acetylcholine transporter ( VAChT) over-expression induces major modifications of striatal cholinergic interneuron morphology and function.
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Janickova, Helena, Prado, Vania F., Prado, Marco A. M., El Mestikawy, Salah, and Bernard, Véronique
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ACETYLCHOLINE , *ACETYLTRANSFERASES , *INTERNEURONS , *CHOLINERGIC receptors , *ELECTRON microscopy , *GENETIC overexpression , *PHYSIOLOGY - Abstract
Striatal cholinergic interneurons ( CIN) are pivotal for the regulation of the striatal network. Acetylcholine ( ACh) released by CIN is centrally involved in reward behavior as well as locomotor or cognitive functions. Recently, BAC transgenic mice expressing channelrhodopsin-2 (ChR2) protein under the control of the choline acetyltransferase (Ch AT) promoter (Ch AT-ChR2) and displaying almost 50 extra copies of the VAChT gene were used to dissect cholinergic circuit connectivity and function using optogenetic approaches. These mice display over-expression of the vesicular acetylcholine transporter ( VAChT) and increased cholinergic tone. Consequently, Ch AT-ChR2 mice are a valuable model to investigate hypercholinergic phenotypes. Previous experiments established that Ch AT-ChR2 mice display an increased sensitivity to amphetamine induced-locomotor activity and stereotypes. In the present report, we analyzed the impact of VAChT over-expression in the striatum of Ch AT-ChR2 mice. Ch AT-ChR2 mice displayed increased locomotor sensitization in response to low dose of cocaine. In addition, we observed a dramatic remodeling of the morphology of CIN in Ch AT-ChR2 transgenic mice. VAChT immunolabeling was markedly enhanced in the soma and terminal of CIN from Ch AT-ChR2 mice as previously shown (Crittenden et al. 2014). Interestingly, the number of cholinergic varicosities was markedly reduced (−87%) whereas their size was significantly increased (+177%). Moreover, VAChT over-expression dramatically modified its trafficking along the somatodendritic and axonal arbor. These findings demonstrate that Ch AT-ChR2 mice present major alterations of CIN neuronal morphology and increased behavioral sensitization to cocaine, supporting the notion that the increased levels of VAChT observed in these mice make them fundamentally different from wild-type mice. [ABSTRACT FROM AUTHOR]
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- 2017
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13. Cholinergic/glutamatergic co-transmission in striatal cholinergic interneurons: new mechanisms regulating striatal computation.
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Kljakic, Ornela, Janickova, Helena, Prado, Vania F., and Prado, Marco A. M.
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PARASYMPATHOMIMETIC agents ,NEUROPEPTIDES ,NEUROTRANSMITTERS ,GLUTAMIC acid ,INTERNEURONS ,PARKINSON'S disease ,NEURONS - Abstract
It is well established that neurons secrete neuropeptides and ATP with classical neurotransmitters; however, certain neuronal populations are also capable of releasing two classical neurotransmitters by a process named co-transmission. Although there has been progress in our understanding of the molecular mechanism underlying co-transmission, the individual regulation of neurotransmitter secretion and the functional significance of this neuronal 'bilingualism' is still unknown. Striatal cholinergic interneurons ( CINs) have been shown to secrete glutamate (Glu) in addition to acetylcholine ( ACh) and are recognized for their role in the regulation of striatal circuits and behavior. Our review highlights the recent research into identifying mechanisms that regulate the secretion and function of Glu and ACh released by CINs and the roles these neurons play in regulating dopamine secretion and striatal activity. In particular, we focus on how the transporters for ACh ( VAChT) and Glu ( VGLUT3) influence the storage of neurotransmitters in CINs. We further discuss how these individual neurotransmitters regulate striatal computation and distinct aspects of behavior that are regulated by the striatum. We suggest that understanding the distinct and complementary functional roles of these two neurotransmitters may prove beneficial in the development of therapies for Parkinson's disease and addiction. Overall, understanding how Glu and ACh secreted by CINs impacts striatal activity may provide insight into how different populations of 'bilingual' neurons are able to develop sophisticated regulation of their targets by interacting with multiple receptors but also by regulating each other's vesicular storage. This is an article for the . [ABSTRACT FROM AUTHOR]
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- 2017
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14. Deletion of the vesicular acetylcholine transporter from pedunculopontine/laterodorsal tegmental neurons modifies gait.
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Janickova, Helena, Rosborough, Kaie, Al‐Onaizi, Mohammed, Kljakic, Ornela, Guzman, Monica S., Gros, Robert, Prado, Marco A. M., and Prado, Vania F.
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PARKINSON'S disease treatment ,PARKINSON'S disease & genetics ,CHOLINERGIC mechanisms ,CELLULAR signal transduction ,MOTOR learning ,CHOLINERGIC receptors - Abstract
Postural instability and gait disturbances, common disabilities in the elderly and frequently present in Parkinson's disease ( PD), have been suggested to be related to dysfunctional cholinergic signaling in the brainstem. We investigated how long-term loss of cholinergic signaling from mesopontine nuclei influence motor behaviors. We selectively eliminated the vesicular acetylcholine transporter ( VAChT) in pedunculopontine and laterodorsal tegmental nuclei cholinergic neurons to generate mice with selective mesopontine cholinergic deficiency ( VACh T
E n1-Cre-flox/flox ). VACh TE n1-Cre-flox/flox mice did not show any gross health or neuromuscular abnormality on metabolic cages, wire-hang and grip-force tests. Young VACh TE n1-Cre-flox/flox mice (2-5 months-old) presented motor learning/coordination deficits on the rotarod; moved slower, and had smaller steps on the catwalk, but showed no difference in locomotor activity on the open field. Old VACh TE n1-Creflox/flox mice (13-16 months-old) showed more pronounced motor learning/balance deficits on the rotarod, and more pronounced balance deficits on the catwalk. Furthermore, old mutants moved faster than controls, but with similar step length. Additionally, old VAChT-deficient mice were hyperactive. These results suggest that dysfunction of cholinergic neurons from mesopontine nuclei, which is commonly seen in PD, has causal roles in motor functions. Prevention of mesopontine cholinergic failure may help to prevent/improve postural instability and falls in PD patients. [ABSTRACT FROM AUTHOR]- Published
- 2017
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15. Cardiac acetylcholine inhibits ventricular remodeling and dysfunction under pathologic conditions.
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Roy, Ashbeel, Dakroub, Mouhamed, Tezini, Geisa C. S. V., Yin Liu, Guatimosim, Silvia, Qingping Feng, Salgado, Helio C., Prado, Vania F., Prado, Marco A. M., and Gros, Robert
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ACETYLCHOLINE ,VENTRICULAR remodeling ,DYSAUTONOMIA ,HEART failure ,HEART cells - Abstract
Autonomic dysfunction is a characteristic of cardiac disease and decreased vagal activity is observed in heart failure. Rodent cardiomyocytes produce de novo ACh, which is critical in maintaining cardiac homeostasis. We report that this nonneuronal cholinergic system is also found in human cardiomyocytes, which expressed choline acetyltransferase (ChAT) and the vesicular acetylcholine transporter (VAChT). Furthermore, VAChT expression was increased 3- and 1.5-fold at the mRNA and protein level, respectively, in ventricular tissue from patients with heart failure, suggesting increased ACh secretion in disease. We used mice with genetic deletion of cardiomyocyte-specific VAChT or ChAT and mice overexpressing VAChT to test the functional significance of cholinergic signaling. Mice deficient for VAChT displayed an 8% decrease in fractional shortening and 13% decrease in ejection fraction compared with angiotensin II (Ang II)-treated control animals, suggesting enhanced ventricular dysfunction and pathologic remodeling in response to Ang II. Similar results were observed in ChAT-deficient mice. Conversely, no decline in ventricular function was observed in Ang II-treated VAChT overexpressors. Furthermore, the fibrotic area was significantly greater (P < 0.05) in Ang II-treated VAChT-deficient mice (3.61 ± 0.64%) compared with wild-type animals (2.24 ± 0.11%). In contrast, VAChT overexpressing mice did not display an increase in collagen deposition. Our results provide new insight into cholinergic regulation of cardiac function, suggesting that a compensatory increase in cardiomyocyte VAChT levels may help offset cardiac remodeling in heart failure. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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16. α7 nicotinic ACh receptor-deficient mice exhibit sustained attention impairments that are reversed by β2 nicotinic ACh receptor activation.
- Author
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Kolisnyk, Benjamin, Al ‐ Onaizi, Mohammed A., Prado, Vania F., Prado, Marco A. M., and Al-Onaizi, Mohammed A
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NICOTINIC receptors ,LABORATORY mice ,PREFRONTAL cortex ,REACTION time ,KNOCKOUT mice ,ATTENTION ,ANIMAL behavior ,CHOLINERGIC receptors ,ANIMAL experimentation ,ANIMALS ,HETEROCYCLIC compounds ,INGESTION ,MICE ,RESEARCH funding ,NICOTINIC agonists ,PHARMACODYNAMICS ,PHYSIOLOGY - Abstract
Background and Purpose: Disruptions of executive function, including attentional deficits, are a hallmark of a number of diseases. ACh in the prefrontal cortex regulates attentive behaviour; however, the role of α7 nicotinic ACh receptor (α7nAChR) in attention is contentious.Experimental Approach: In order to probe attention, we trained both wild-type and α7nAChR knockout mice on a touch screen-based five-choice serial reaction time task (5-CSRT). Following training procedures, we then tested sustained attention using a probe trial experiment. To further differentiate the role of specific nicotinic receptors in attention, we then tested the effects of both α7nAChR and β2nAChR agonists on the performance of both wild-type and knockout mice on the 5-CSRT task.Key Results: At low doses, α7nAChR agonists improved attentional performance of wild-type mice, while high doses had deleterious effects on attention. α7nAChR knockout mice displayed deficits in sustained attention that were not ameliorated by α7nAChR agonists. However, these deficits were completely reversed by the administration of a β2nAChR agonist. Furthermore, administration of a β2nAChR agonist in α7nAChR knockout mice elicited similar biochemical response in the prefrontal cortex as the administration of α7nAChR agonists in wild-type mice.Conclusions and Implications: Our experiments reveal an intricate relationship between distinct nicotinic receptors to regulate attentional performance and provide the basis for targeting β2nAChRs pharmacologically to decrease attentional deficits due to a dysfunction in α7nAChRs. [ABSTRACT FROM AUTHOR]- Published
- 2015
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17. Neuroanatomical and cognitive biomarkers of alpha‐synuclein propagation in a mouse model of synucleinopathy prior to onset of motor symptoms.
- Author
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Tullo, Stephanie, Miranda, Aline S., Cid‐Pellitero, Esther, Lim, Mei Peng, Gallino, Daniel, Attaran, Anoosha, Patel, Raihaan, Novikov, Vladislav, Park, Megan, Beraldo, Flavio H., Luo, Wen, Shlaifer, Irina, Durcan, Thomas M., Bussey, Timothy J., Saksida, Lisa M., Fon, Edward A., Prado, Vania F., Prado, Marco A. M., and Chakravarty, M. Mallar
- Abstract
Significant evidence suggests that misfolded alpha‐synuclein (aSyn), a major component of Lewy bodies, propagates in a prion‐like manner contributing to disease progression in Parkinson's disease (PD) and other synucleinopathies. In fact, timed inoculation of M83 hemizygous mice with recombinant human aSyn preformed fibrils (PFF) has shown symptomatic deficits after substantial spreading of pathogenic alpha‐synuclein, as detected by markers for the phosphorylation of S129 of aSyn. However, whether accumulated toxicity impact human‐relevant cognitive and structural neuroanatomical measures is not fully understood. Here we performed a single unilateral striatal PFF injection in M83 hemizygous mice, and using two assays with translational potential, ex vivo magnetic resonance imaging (MRI) and touchscreen testing, we examined the combined neuroanatomical and behavioral impact of aSyn propagation. In PFF‐injected mice, we observed widespread atrophy in bilateral regions that project to or receive input from the injection site using MRI. We also identified early deficits in reversal learning prior to the emergence of motor symptoms. Our findings highlight a network of regions with related cellular correlates of pathology that follow the progression of aSyn spreading, and that affect brain areas relevant for reversal learning. Our experiments suggest that M83 hemizygous mice injected with human PFF provides a model to understand how misfolded aSyn affects human‐relevant pre‐clinical measures and suggest that these pre‐clinical biomarkers could be used to detect early toxicity of aSyn and provide better translational measures between mice and human disease. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
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