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In pursuit of the "double carbon" objectives, converting high-carbon thermal power plants into carbon capture power plants is recognized as an effective measure to mitigate carbon emissions. In order to improve the economy of the system, an electric hydrogen generation unit is introduced, and an economic dispatch strategy that considers the combination of a flexible carbon capture power plant and electric hydrogen generation is proposed. Firstly, the low-carbon principle of the flexible carbon capture plant is introduced, and secondly, the joint operation structure of the flexible carbon capture plant, pumped storage, and hydrogen generation is constructed in order to fully exploit the synergistic operation potential of the hydrogen and carbon capture plants. On this basis, an economic dispatch model with the objective of the lowest combined system cost of the sum of system operation cost, system carbon trading cost, hydrogen production revenue, and wind and light abandonment cost is established, fuzzy parameters are used to characterize the uncertainty levels of wind and light, fuzzy opportunity constraints are established, and the model is solved using an improved sparrow search algorithm. The results of the example show that the proposed model reduces the comprehensive cost by 20.9% and carbon emissions by 61.77% compared with the existing model, which has significant low-carbon economic benefits. [ABSTRACT FROM AUTHOR]

Main conclusion: Differentially expressed microRNAs were found associated with the development of chasmogamous and cleistogamous flowers in Viola prionantha, revealing potential roles of microRNAs in the developmental evolution of dimorphic flowers. In Viola prionantha, chasmogamous (CH) flowers are induced by short daylight, while cleistogamous (CL) flowers are triggered by long daylight. How environmental factors and microRNAs (miRNAs) affect dimorphic flower formation remains unknown. In this study, small RNA sequencing was performed on CH and CL floral buds at different developmental stages in V. prionantha, differentially expressed miRNAs (DEmiRNAs) were identified, and their target genes were predicted. In CL flowers, Viola prionantha miR393 (vpr-miR393a/b) and vpr-miRN3366 were highly expressed, while in CH flowers, vpr-miRN2005, vpr-miR172e-2, vpr-miR166m-3, vpr-miR396f-2, and vpr-miR482d-2 were highly expressed. In the auxin-activated signaling pathway, vpr-miR393a/b and vpr-miRN2005 could target Vpr-TIR1/AFB and Vpr-ARF2, respectively, and other DEmiRNAs could target genes involved in the regulation of transcription, e.g., Vpr-AP2-7. Moreover, Vpr-UFO and Vpr-YAB5, the main regulators in petal and stamen development, were co-expressed with Vpr-TIR1/AFB and Vpr-ARF2 and showed lower expression in CL flowers than in CH flowers. Some V. prionantha genes relating to the stress/defense responses were co-expressed with Vpr-TIR1/AFB, Vpr-ARF2, and Vpr-AP2-7 and highly expressed in CL flowers. Therefore, in V. prionantha, CH–CL flower development may be regulated by the identified DEmiRNAs and their target genes, thus providing the first insight into the formation of dimorphic flowers in Viola. [ABSTRACT FROM AUTHOR]

Carbon emission trading scheme (ETS) policy is a quota-based market-motivated environmental regulation policy that by transforming environmental responsibility of enterprises into self-consciousness behavior induces green innovation behavior in enterprises and influences the level of regional green innovation. Based on panel data on green patents in 30 Chinese provinces from 2010 to 2019, the paper uses six Chinese pilot provinces for ETS as the treatment group to investigate the induced effects of Chinese ETS policy on the quantity and quality of green technology innovation using difference in difference model and analyzes the effects of Chinese ETS policy on green technology innovation in different pilot regions using a synthetic control method. The findings indicate that (1) compared with non-pilot areas, the carbon emission trading policies in pilot areas can induce green technology innovation activities. (2) In all pilot regions, ETS policies significantly promoted the quantity and quality of green technology innovation, but the quantity promotion effect was significantly better than the quality promotion effect, and the low-quality promotion effect was better than the high-quality promotion effect. (3) In terms of individual pilot region, ETS policies in Guangdong, Hubei and Tianjin significantly promoted the quantity of green technology innovation and low-quality green technology innovation, but significantly inhibited or suppressed high-quality green technology innovation in a short term. This paper not only enriches the research on environmental equity trading policies and green technology innovation theoretically, but also provides empirical evidence for the effectiveness of carbon emission trading schemes pilots program in China. [ABSTRACT FROM AUTHOR]

In the process of yielding biofuels from cellulose degradation, traditional enzymatic hydrolysis, such as β-glucosidase catalyzing cellobiose, can barely resolve the contradiction between cellulose degradation and bioenergy conservation. However, it has been shown that cellobiose phosphorylase provides energetic advantages for cellobiose degradation through a phosphorolytic pathway, which has attracted wide attention. Here, the cellobiose phosphorylase gene from Caldicellulosiruptor bescii (CbCBP) was cloned, expressed, and purified. Analysis of the enzymatic properties and kinetic mechanisms indicated that CbCBP catalyzed reversible phosphorolysis and had good thermal stability and broad substrate selectivity. In addition, the phosphorolytic reaction of cellobiose by CbCBP proceeded via an ordered Bi Bi mechanism, while the synthetic reaction proceeded via a ping pong Bi Bi mechanism. The present study lays the foundation for optimizing the degradation of cellulose and the synthesis of functional oligosaccharides. [ABSTRACT FROM AUTHOR]

Purpose: Our purpose was to explore pupil light response (PLR) with respect to the change in sensitivity of photoreceptors during various dark adaptation phases and to determine the optimal duration of dark adaptation time before the PLR. Methods: The PLR was recorded in 15 healthy subjects and three patients with neural or retinal vision loss after 1-sec blue and red light stimuli of 1, 10, and 100 cd/m. The PLR was repeated nine times at different checkpoints during the 40-minute dark adaptation. The transient contraction amplitude, sustained contraction amplitude, and relative sustained contraction ratio of the PLR were analyzed. Results: The increase in the transient contraction amplitude during the entire dark adaptation process was significant (changing up to 45.1 %) in the initial phase of dark adaptation under different stimulus conditions. The changes in the sustained contraction amplitude and the relative sustained contraction ratio were substantial (changing up to 71.0 % and 37.2 % from 1 to 20 minutes of dark adaptation, respectively) under high-intensity blue illumination. The inflection point of the contraction curves in the dark adaptation was 15 or 20 minutes. The patients' PLR results changed in a similar manner. Conclusions: The changes in the sensitivity of different photoreceptors occurred at different rates, and the contraction amplitude of the PLR was significantly affected by the dark adaptation duration. So 20 minutes of dark adaptation before PLR testing was suggested to achieve a consistent and stable pupil response. The dark adaptation effect should be put into consideration when comparing the results from different phases of the PLR test. [ABSTRACT FROM AUTHOR]