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Background: Vocal fold (VF) scarring remains a therapeutic dilemma and challenge in modern laryngology. To facilitate corresponding research, we aimed to establish an in vitro fibrogenesis model employing human VF fibroblasts (hVFF) and the principles of macromolecular crowding (MMC).Methods: Fibrogenesis was promoted by addition of transforming growth factor-β1 to standard medium and medium containing inert macromolecules (MMC). Hepatocyte growth factor (HGF) and Botox type A were tested for their antifibrotic properties in various doses. Experiments were analyzed with respect to the biosynthesis of collagen, fibronectin, and α-smooth muscle actin using immunofluorescence, silver stain and western blot.Results: MMC led to favourable enhanced deposition of collagen and other extracellular matrix components, reflecting fibrotic conditions. Low doses of HGF were able to dampen profibrotic effects. This could not be observed for higher HGF concentrations. Botox type A did not show any effects.Conclusion: Based on the principles of MMC we could successfully establish a laryngeal fibrogenesis model employing hVFF. Our finding of dose-dependent HGF effects is important before going into clinical trials in humans and has never been shown before. Our model provides a novel option to screen various potential antifibrotic compounds under standardized conditions in a short time. [ABSTRACT FROM AUTHOR]

This study of values placed on wildlife by Bantu and Yaka Pygmy forest dwellers ( n = 200) in Northern Congo identified and analyzed two wildlife value orientations - 'anthropocentric' and 'biocentric.' The former, strongly displayed across all segments of both societies, was likely motivated by heavy reliance on bushmeat and human-wildlife conflicts, and was linked to attitudes approving the killing of animals for human benefit. The 'biocentric' orientation was more common among formally educated male respondents who do not hunt and positively linked to attitudes favoring conservation. Wildlife management strategies should consider including 1) sustainable local wildlife exploitation, 2) livelihood projects that provide a real alternative to hunting, and 3) human-wildlife conflict mitigation. [ABSTRACT FROM AUTHOR]