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Start Over Topic adsorption Publication Year Range Last 10 years Publisher springer berlin heidelberg
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30. Parallelized label-free monitoring of cell adhesion on extracellular matrix proteins measured by single colour reflectometry

Author

Johanna Hutterer, Günter Gauglitz, Günther Proll, and Peter Fechner

Subjects
Materials science, Surface Properties, Phase (waves), Color, Biocompatible Materials, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Multiplexing, Direct optical sensing, Analytical Chemistry, Extracellular matrix, Adsorption, Cell adhesion, Reflectometry, Extracellular Matrix Proteins, Cell-substrate interaction, Adhesion, Single colour reflectometry (SCORE), 021001 nanoscience & nanotechnology, 0104 chemical sciences, Extracellular Matrix, Transducer, 0210 nano-technology, Biomedical engineering, Research Paper
Abstract

The understanding of the initial cell adhesion to biomaterials is crucial for the survival of implants. The manifold possibilities to tailor an implant surface and the diverse requirements for different implant applications necessitate a timesaving and highly parallelized analytical methodology. Due to its intrinsic advantages (label-free, time-resolved, robust against temperature fluctuations, and particularly the multiplexing possibilities), single colour reflectometry (SCORE) is used for the first time to investigate cell adhesion to different extracellular matrix protein–coated surfaces. The excellent correlation between the novel SCORE technology and well-established reference methods proves that the results obtained by using this direct optical method are able to reflect the cell binding processes at the transducer surface. Additionally, the high time resolution of SCORE revealed the differences in the adhesion behaviour of the cells on the different extracellular matrix protein–coated glass slides during the initial adsorption phase and during the spreading of the cells on the surfaces. Therefore, we conclude that SCORE is a perfectly suited methodology for studying the entire cell adsorption process, including morphological changes, and shows great potential for other cell-based sensing applications.Graphical abstract

Published
2021