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Objective To study the variation of E6 and E7 genes in HPV16 mutant strains in Panxi region of China and their genetic relationship with the worlds major HPV16 mutant lineages. Methods Womens cervical cell samples were collected and genotyped in Affiliated Hospital of Panzhihua University. The genetic sequences of E6 and E7 in HPV16 from 57 specimens were detected and analyzed in a molecular phylogenetic analysis. Results HPV16 variants in Panxi region belong to Asian or European mutant lineage. Among them. 16 types of nucleotide mutations were observed from E6 and E7 genes.including 9 missense mutations and 7 synonymous mutations. The mutation rate of E6 gene was 12.3% (7/57),and all 6 mutations in E6 gene were missense mutations. E7 gene had a mutation rate of 94.7% (54/57) and involved three missense mutations. A86G mutation in E7 gene may significantly change the carcinogenic ability of HPV16. Conclusion In Panxi region,the mutation types of E6 and E7 in HPV16 are a-bundant. All HPV16 mutants in Panxi region belong to European or Asian lineage and have many branches in their molecular phylogenetic tree. [ABSTRACT FROM AUTHOR]

目的了解婴儿期下呼吸道感染病原菌分布及耐药性,为临床合理选用抗菌药物提供依据。方法回顾性统计2016年1月至2018年12月该院收治的1516份婴儿期下呼吸道感染者痰标本分离的病原菌种类,并对主要菌种的耐药性进行分析。结果共检出病原菌677株,其中革兰阴性菌564株(83.31%),位于前3位的由高至低依次为流感嗜血杆菌、肺炎克雷伯菌、大肠埃希菌;革兰阳性菌99株(14.62%),分别为金黄色葡萄球菌和肺炎链球菌。流感嗜血杆菌对氨苄西林耐药率大于50.00%,对氨曲南、头孢吡肟、头孢曲松、氯霉素、氧氟沙星耐药率小于10.00%;肺炎克雷伯菌、大肠埃希菌对氨苄西林耐药率大于90.00%,对头孢哌酮/舒巴坦、哌拉西林/他唑巴坦、头孢西丁、阿米卡星、亚胺培南、美罗培南耐药率小于10.00%;金黄色葡萄球菌对青霉素耐药率达98.89%,对红霉素、克林霉素、复方新诺明耐药率大于50.00%,对万古霉素100.00%敏感。结论该院婴儿期下呼吸道感染病原菌以革兰阴性菌为主,细菌耐药较为普遍。临床上应根据婴儿期用药特点及药敏试验结果合理使用抗菌药物。 [ABSTRACT FROM AUTHOR]

现阶段，因部分医务人员医德医风缺失，导致医患关系紧张，影响了医院的形象及发展。该文综合分析了医务人员医德医风缺失的主要原因，包括市场经济影响、医疗服务市场的特殊性、医院管理因素及个人因素等，并提出加强医德医风教育、开展督查、落实奖惩、建立服务基层制度等对策。 [ABSTRACT FROM AUTHOR]

目的 牙周病病例的种植治疗由于骨量受损和有限的牙间隙而较难种植, 本研究的目的是评估三维计算机虚拟 引导技术在牙周病种植术中的临床应用效果。方法 对 2018 年 8 月至 2019 年 4 月该院收治的16名患有牙周病的 32 个拟种植 部位进行完整的数字化工作流程,通过计算机进入点(肩)和顶点(尖端)的坐标及计划植入物和实际植入物的角度偏差来评估准 确性。结果 用计算机设计虚拟引导技术在牙周病种植中可以很好地植入种植体,角度的偏离值为 (4.53±1.89)°, 根部的偏离 值为 (0.97±0.21)mm, 肩部的偏离值为 (0.85±0.19)mm。结论 三维虚拟引导技术的应用有助于提高骨量不足和牙间隙有限 的牙周病患者的种植精准度。 [ABSTRACT FROM AUTHOR]