1. Altered mitochondrial proteome and functional dynamics in patients with rheumatoid arthritis.
- Author
-
Khanna, Shweta, Padhan, Prasanta, Jaiswal, Kumar S., Jain, Ankit P., Ghosh, Arup, Tripathy, Archana, Gowda, Harsha, Raghav, Sunil K., and Gupta, Bhawna
- Subjects
- *
RHEUMATOID arthritis , *MEMBRANE potential , *SUPEROXIDES , *MITOCHONDRIAL proteins , *MITOCHONDRIAL membranes , *LIQUID chromatography-mass spectrometry - Abstract
• Mitochondrial proteins are differentially expressed in RA PBMCs as compared to healthy controls. • Differentially expressed proteins are part of OXPHOS and mitochondrial dysfunction. • Mitochondrial membrane potential, superoxide levels and total cellular ATP are reduced in RA PBMCs. The autoimmune inflammatory disease, Rheumatoid arthritis (RA), has known imbalances in energy metabolism and superoxide levels thus may have an etiology associated with mitochondrial dysfunction. We thus evaluated the presence of a differential mitochondrial proteome as well as other characteristics including mitochondrial mass, membrane potential (Ψm), total cellular ATP and superoxide levels. Eighteen mitochondrial proteins were down-regulated while four were up-regulated in RA patients in comparison to the healthy controls (HC). A significant decrease in mitochondrial Ψm, superoxides and cellular ATP levels was observed in RA with constant mitochondrial mass suggesting mitochondrial dysfunction responsible for functional disparity in RA. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF