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Your search keyword '"Basu, Shibom"' showing total 2 results
Start Over Author "Basu, Shibom" Publication Year Range Last 10 years Publisher american association for the advancement of science
2 results on '"Basu, Shibom"'

1. Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.

Author

Tenboer, Jason, Basu, Shibom, Zatsepin, Nadia, Pande, Kanupriya, Milathianaki, Despina, Frank, Matthias, Hunter, Mark, Boutet, Sébastien, Williams, Garth J., Koglin, Jason E., Oberthuer, Dominik, Heymann, Michael, Kupitz, Christopher, Conrad, Chelsie, Coe, Jesse, Roy-Chowdhury, Shatabdi, Weierstall, Uwe, James, Daniel, Wang, Dingjie, and Grant, Thomas

Subjects
*INTERMEDIATES (Chemistry), *PHOTOACTIVE yellow protein, *PROTEIN structure, *CRYSTALLOGRAPHY, *TIME-resolved measurements, *ELECTRON density, *HIGH resolution imaging, *SYNCHROTRONS
Abstract

The article discusses the capture of high-resolution intermediates of photoactive yellow protein (PYP), a bacterial blue light photoreceptor, with time-resolved serial femtosecond crystallography (TR-SFX), which uses ultrashort pulses from x-ray free electron lasers (XFELs). Topics include the challenges of TR-SFX, TR-SFX's advantages over synchrotron-based, time-resolved Laue crystallography, and difference electron density (DED) maps created by using microcrystals of PYP as a model system.

Published
2014
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2. Femtosecond structural dynamics drives the trans/cis isomerization in photoactive yellow protein.

Author

Pande, Kanupriya, Hutchison, Christopher D. M., Groenhof, Gerrit, Aquila, Andy, Robinson, Josef S., Tenboer, Jason, Basu, Shibom, Boutet, Sébastien, DePonte, Daniel P., Mengning Liang, White, Thomas A., Zatsepin, Nadia A., Yefanov, Oleksandr, Morozov, Dmitry, Oberthuer, Dominik, Gati, Cornelius, Subramanian, Ganesh, James, Daniel, Yun Zhao, and Koralek, Jake

Subjects
*ISOMERIZATION, *PHOTOACTIVE yellow protein, *PROTEIN structure, *FEMTOSECOND pulses, *PHOTONS
Abstract

A variety of organisms have evolved mechanisms to detect and respond to light, in which the response is mediated by protein structural changes after photon absorption. The initial step is often the photoisomerization of a conjugated chromophore. Isomerization occurs on ultrafast time scales and is substantially influenced by the chromophore environment. Here we identify structural changes associated with the earliest steps in the trans-to-cis isomerization of the chromophore in photoactive yellow protein. Femtosecond hard x-ray pulses emitted by the Linac Coherent Light Source were used to conduct time-resolved serial femtosecond crystallography on photoactive yellow protein microcrystals over a time range from 100 femtoseconds to 3 picoseconds to determine the structural dynamics of the photoisomerization reaction. [ABSTRACT FROM AUTHOR]

Published
2016
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