1. Spatio-temporal programming of lyotropic phase transition in nanoporous microfluidic confinements.
- Author
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Ulaganathan, Vamseekrishna and Sengupta, Anupam
- Subjects
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PHASE transitions , *LYOTROPIC liquid crystals , *CRYSTAL models , *SURFACE topography , *BIOMATERIALS , *IMAGE processing - Abstract
[Display omitted] The nanoporous polydimethylsiloxane (PDMS) surfaces of a rectangular microfluidic channel, selectively uptakes water molecules, concentrating the solute molecules in an aqueous phase, that could drive phase transitions. Factors such as surface wettability, channel geometry, the surface-to-volume ratio, and surface topography of the confinements could play a key role in tuning the phase transitions spatio-temporally. Using a lyotropic chromonic liquid crystal as model biological material, confined within nanoporous microfluidic environments, we study molecular assembly driven by nanoporous substrates. By combining timelapse polarized imaging, quantitative image processing, and a simple mathematical model, we analyze the phase transitions and construct a master diagram capturing the role of surface wettability, channel geometry and embedded topography on programmable lyotropic phase transitions. Intrinsic PDMS nanoporosity and confinement cross-section, together with the imposed wettability regulate the rate of the N -M phase transition; whereas the microfluidic geometry and embedded topography enable phase transition at targeted locations. We harness the emergent long-range order during N -M transition to actuate elasto-advective transport of embedded micro-cargo, demonstrating particle manipulation concepts governed by tunable phase transitions. Our results present a programmable physical route to material assembly in microfluidic environment, and offer a new paradigm for assembling genetic components, biological cargo, and minimal synthetic cells. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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