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This book presents key aspects and recent developments of cryogenic sample electron tomography (cryo-ET) methodology, authored by leading experts in the field. Understanding structure and function of biomolecules in the context of cells is a new frontier in cellular and structural biology. To facilitate such research, cryo-ET is a key method to visualize the molecules of life in their native settings. Cryo-ET enables the imaging of samples that are preserved in a near-native state, at (macro)-molecular resolution and in three dimensions. Thus, this technique is a unique tool to gain insights into how biomolecules collaborate in orchestrating fundamental biological processes, how mutations cause diseases, pathogens cause infections, and to develop novel therapeutics to treat such illnesses. This book provides a unique reference for the emerging field of cryo-ET. The topics covered range from the fundamental principles of imaging to sample preparation, data analysis,and data sharing within the scientific community. It serves as a valuable resource for the next generation of structural biologists, making it suitable both for undergraduate students studying biochemistry, biophysics, and molecular biology and highly valuable for the more experienced and specialized PhD student. Furthermore, it stands as a state-of-the–art source of knowledge for the established senior scientist within the field of structural biology.

This book provides an overview of the available evidence surrounding immunotherapy in gastrointestinal cancers, and discusses its future place in clinical practice.Immunotherapy has celebrated some astonishing therapeutic successes in a variety of cancer types and is becoming increasingly relevant in daily clinical practice. Currently, the predominant class of immunotherapeutic drugs is the so-called checkpoint inhibitors, which disengage the physiological brakes on the immune system, enabling a more effective anti-cancer immune response. Malignancies of the gastrointestinal tract, which account for the majority of cancer cases worldwide, are a major cause of morbidity and mortality, creating an urgent need for more effective therapies. A large number of clinical trials have evaluated the efficacy of cancer immunotherapy in gastrointestinal malignancies and demonstrated its potential in certain subsets of patients. This book will appeal to a wide readership, including oncologists, health care professionals in general and biomedical scientists.

Alle geringfügigen Beschäftigungsverhältnisse müssen wie andere Beschäftigungsverhältnisse der Sozialversicherung gemeldet werden. Legt der Arbeitnehmer dem Arbeitgeber zudem eine Lohnsteuerkarte vor, ist der Lohnsteuerabzug nach den Merkmalen der Lohnsteuerkarte vorzunehmen. Damit ergeben sich zahlreiche Beratungsfelder: - Besteuerung der 400 Euro Mini-Jobs - Beschäftiung in Haushalten - Kurzfristige Beschäftigungen - Besteuerung von Gleitzonen-Verhältnissen - Beitragsrecht - Arbeitsrecht

Als Reisekosten werden im deutschen Steuerrecht Aufwendungen bezeichnet, die bei beruflich oder betrieblich bedingten Reisen anfallen. Das Reisekostenrecht hat dabei in jüngster Zeit zahlreiche Änderungen erfahren, welche in der Beratung und Bearbeitung neue Überlegungen zur Steueroptimierung erfordern. Das Werk erläutert das neue Reisekostenrecht mit folgenden Schwerpunkten: - Übernachtungskosten - Verpflegungsmehraufwand - Reisenebenkosten - Reisekostenerstattung nach § 3 Nr. 13 bzw. Nr. 16 EStG - Einzelnachweis und Pauschbeträge - Kilometerpauschale - Betriebliche Fahrzeuge Ein echtes Praxiswerk für den schnellen Zugriff zum Reisekostenrecht.