Search

Wetlands are the most biologically diverse ecosystems on Earth. The isolation of Streptomyces strains from wetlands is helpful to study their diversity and functions in such habitats. In this study, six strains of Streptomyces were isolated from the rhizosphere soil of three plant species in the Huaxi Wetland at Guiyang and were identified as Streptomyces galilaeus, S. avidinii, S. albogriseolus, S. albidoflavus, S. spororaveus, and S. cellulosae, respectively. The six strains all solubilized phosphate, fixed nitrogen, and produced ACC deaminase and siderophores, and four strains also secreted indole-3-acetic acid. The six strains had the ability to resist to certain degrees of salinity, drought, and acidic/alkaline pH stress. In addition, the S. avidinii WL3 and S. cellulosae WL9 strains significantly promoted seed germination of mung bean, pepper, and cucumber, especially the WL3 strain. A pot experiment further showed that WL3 significantly promoted the growth of cucumber seedlings. Thus, strains of six species of Streptomyces with multiple plant growth-promoting characteristics were isolated from the wetland. These results lay a foundation for their potential use as microbial agents for seed-coating treatments. [ABSTRACT FROM AUTHOR]

Small‐leaved Kuding tea (SLKDT) obtained from Ligustrum robustum is a traditional tea substitute in southern China and has a range of physiological effects. However, the changes in its phytochemical composition after various heat treatments are not reported yet. Thus, the phytochemical composition and antioxidant activities of fresh leaves of SLKDT (LrF1) and SLKDT after high‐temperature wet‐heat treatment (LrF2) and wet‐ and dry‐heat treatments (LrF3) were assessed using liquid chromatography–mass spectrometry, and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonate (ABTS) and 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging activities and lipid peroxidation inhibition activity of LrF1 and LrF3 were investigated. The results indicated that the phytochemical composition of LrF1, LrF2, and LrF3 was significantly different. Overall, 258 and 83 differential constituents, respectively, were obtained in LrF1 versus LrF2 and LrF2 versus LrF3. The differential constituents mainly included amino acids and their derivatives, nucleosides, flavonoids, terpenoids, simple phenylpropanoids, and coumarins. After heat treatment, SLKDT exhibited obvious changes in sensory characteristics and physiological properties, which may be related to the changes in the levels of amino acids, linalool, beta‐geraniol, myricetin, naringin, fraxetin, and isoacteoside. Moreover, the antioxidant activities significantly changed after heat treatment of SLKDT. Overall, our study demonstrated that heat treatment can alter the phytochemical composition of SLKDT, thus affecting its sensory properties and physiological properties. Practical Application: This study preliminarily assessed the changes in the composition of small‐leaved Kuding tea (SLKDT) after various heat treatments and revealed that the composition of SLKDT tea can be adjusted by various heat and temperature treatments. [ABSTRACT FROM AUTHOR]

Due to the small hydrated ionic radius and light molar mass of ammonium ions, aqueous ammonium ion batteries attract much attention, providing high security, environmental friendliness and low cost. However, the lack of suitable electrode materials with high specific capacity is a big challenge for practical application. Therefore, in view of this problem, we fabricated an anode applying a MoS2 material with a ball-flower morphology anchored to MXene nanoflakes, and it shows excellent rate capability in a novel aqueous ammonium ion battery. The corresponding charge capacities of composite electrodes are 279.2, 204.4, 173.2, 118.7, and 80.5 mA h g−1 at 20, 50, 100, 200, and 500 mA g−1, respectively. Meanwhile, polyvanadate was selected as a cathode material for a full aqueous ammonium ion battery, and interestingly it was discovered that the size of this material decreases with increasing synthesis temperature. The discharge capacities of NH4V4O10 electrodes fabricated at 140 °C, 160 °C, and 180 °C at 50 mA g−1 are 88.6, 125.1 and 155.5 mA h g−1, respectively. Furthermore, we also explore the corresponding electrochemical mechanism using XRD and XPS. A full aqueous ammonium ion battery based on both electrodes shows superior ammonium ion storage properties and provides new ideas for the development of this strategy. [ABSTRACT FROM AUTHOR]

Remediation of mercury (Hg)-contaminated soil by mycorrhizal technology has drawn increasing attention because of its environmental friendliness. However, the lack of systematic investigations on arbuscular mycorrhizal fungi (AMF) community composition in Hg-polluted soil is an obstacle for AMF biotechnological applications. In this study, the AMF communities within rhizosphere soils from seven sites from three typical Hg mining areas were sequenced using an Illumina MiSeq platform. A total of 297 AMF operational taxonomic units (OTUs) were detected in the Hg mining area, of which Glomeraceae was the dominant family (66.96%, 175 OTUs). AMF diversity was significantly associated with soil total Hg content and water content in the Hg mining area. Soil total Hg showed a negative correlation with AMF richness and diversity. In addition, the soil properties including total nitrogen, available nitrogen, total potassium, total phosphorus, available phosphorus, and pH also affected AMF diversity. Paraglomeraceae was found to be negatively correlated to Hg stress. The wide distribution of Glomeraceae in Hg-contaminated soil makes it a potential candidate for mycorrhizal remediation. [ABSTRACT FROM AUTHOR]

Traditional lightning detection and location networks use the time of arrival (TOA) technique to locate lightning events with a single time stamp. This contribution introduces a simulation study to lay the foundation for new lightning location concepts. Here, a novel interferometric method is studied which expands the data use and maps lightning events into an area by using coherency. The amplitude waveform bank, which consists of averaged waveforms classified by their propagation distances, is first used to test interferometric methods. Subsequently, the study is extended to individual lightning event waveforms. Both amplitude and phase coherency of the analytic signal are used here to further develop the interferometric method. To determine a single location for the lightning event and avoid interference between the ground wave and the first skywave, two solutions are proposed: (1) use a small receiver network and (2) apply an impulse response function to the recorded waveforms, which uses an impulse to represent the lightning occurrence. Both methods effectively remove the first skywave interference. This study potentially helps to identify the lightning ground wave without interference from skywaves with a long-range low frequency (LF) network. It is planned to expand the simulation work with data reflecting a variety of ionospheric and geographic scenarios. [ABSTRACT FROM AUTHOR]

Carbon nanohoop compounds have attracted extensive attention recently due to their aesthetically pleasing structures and unique properties. In order to reveal the structure–property relationship between the size-dependent effect and the nonlinear optical (NLO) response, a series of carbon nanohoops consisting of zigzag carbon nanotubes (CNTs), named D-CM2PnP-A (n = 4, 6, 8, 10, and 12), are systematically designed and investigated using density functional theory (DFT). Significantly, the first hyperpolarizability (βtot) of the system increases with the increasing structure size, which is primarily the result of the charge transfer transition. Subsequently, the major electronic transition changes from local excitation to charge transfer as the size of the structure increases, indicating that charge transfer becomes stronger significantly. Furthermore, a two-level model and the absorption spectrum can well explain the trend of the βtot value. In addition, the unit sphere representation (USR) and hyper-Rayleigh scattering (HRS) are used to further explain the nature of the second-order NLO response. More importantly, adding a π-bridge as an effective strategy further enhances the second-order NLO responses of the system. The relevant results are expected to provide a new perspective on the structure–NLO property correlation of donor (D)–conjugated bridge (π)–acceptor (A) type carbon nanohoops and theoretical guidance for the rational design and synthesis of fascinating carbon nanohoops as NLO materials. [ABSTRACT FROM AUTHOR]

Giant planets embedded in protoplanetary disks (PPDs) can create annulus density gaps around their orbits in the type-II regime, potentially responsible for the ubiquity of annular substructures observed in PPDs. Although a substantial amount of works studying type-II planetary migration and gap properties have been published, they have almost exclusively all been conducted under the viscous accretion disk framework. However, recent studies have established magnetized disk winds as the primary mechanism driving disk accretion and evolution, which can coexist with turbulence from the magnetorotational instability (MRI) in the outer PPDs. We conduct a series of 3D global nonideal magnetohydrodynamic (MHD) simulations of type-II planet–disk interactions applicable to the outer PPDs. Our simulations properly resolve the MRI turbulence and accommodate the MHD disk wind. We found that the planet triggers the poloidal magnetic flux concentration around its orbit. The concentrated magnetic flux strongly enhances angular momentum removal in the gap, which is along the inclined poloidal field through a strong outflow emanating from the disk surface outward to the planet gap. The resulting planet-induced gap shape is more similar to an inviscid disk, while being much deeper, which can be understood from a simple inhomogeneous wind torque prescription. The corotation region is characterized by a fast trans-sonic accretion flow that is asymmetric in azimuth about the planet and lacking the horseshoe turns, and the meridional flow is weakened. The torque acting on the planet generally drives inward migration, though the migration rate can be affected by the presence of neighboring gaps through stochastic, planet-free magnetic flux concentration. [ABSTRACT FROM AUTHOR]

[Display omitted] • Incorporated Ni induces the formation of highly active (1 0 1) facet of Ru. • Incorporated Ni restrains the agglomeration of Ru nanocrystals. • Incorporated Ni modulates the electron structure of Ru and increases active sites. • Incorporated Ni endows Ru nanocrystals with very low HER overpotentials. Constructing highly active electrocatalysts towards hydrogen evolution reaction (HER) in both alkaline and acidic media is essential for achieving a sustainable energy economy. Here, a facile ethylene glycol reduction strategy was employed to design the nickel-ruthenium nanocrystals (Ni-Ru NC) with an exposed highly active Ru (1 0 1) facet as an efficient electrocatalyst for HER. Testings show Ni-Ru NC outperforms the benchmark catalyst Pt/C by delivering extraordinarily low overpotentials of 21.1 and 70.9 mV to drive 10 mA cm−2 in acidic and alkaline solutions, respectively. The results of experimental and theoretical studies suggest that Ni can modulate the electronic structure of the Ru NC and optimize the hydrogen adsorption free energy on Ru's surface, which accelerates the charge transfer kinetics and enhances the HER performance. The study support the potential application of facet-modulated Ru-based HER eleccatalyst in an alkaline environment. [ABSTRACT FROM AUTHOR]

Intergenerational relationships have become increasingly crucial for maintaining well-being in aging families. Under a changing sociocultural background, families in Hong Kong increasingly exhibit diverse intergenerational relationships and functioning. Focusing on families with mutiple children, this study investigated how the characteristics of parents and their adult children jointly affect different domains of intergenerational relationship quality. A two-stage stratified random sampling design was adopted. Face-to-face questionnaire interviews were conducted between November 2016 and March 2017 with 1,001 Hong Kong residents aged ≥ 50 years. Data of 612 parents and 1,745 adult children were included for analysis. Hierarchical linear modeling was performed to examine child- and parent-level correlates of intergenerational relationship quality. Parents who were female, were married, had higher self-perceived economic status, owned a house, and had fewer depressive symptoms, exhibited higher intergenerational relationship quality. Parents' age was positively related to affectual closeness, whereas their educational level was negatively related to both affectual closeness and conflict. More favorable intergenerational relationships were reported by aging parents whose adult children were younger, female, and married. Children with higher educational levels exhibited higher levels of both affectual closeness and conflict with their parents. Moreover, affectual closeness was found to be transmitted between generations. The findings can help improve awareness of the factors affecting the different domains of intergenerational relationships, thus informing the development of targeted services and interventions to promote family relationships and well-being. [ABSTRACT FROM AUTHOR]

Clinically, thyroid-related diseases such as endemic iodine deficiency and congenital hypothyroidism are associated with hearing loss, suggesting that thyroid hormones are essential for the development of normal hearing. Triiodothyronine (T3) is the main active form of thyroid hormone and its effect on the remodeling of the organ of Corti remain unclear. This study aims to explore the effect and mechanism of T3 on the remodeling of the organ of Corti and supporting cells development during early development. In this study, mice treated with T3 at postnatal (P) day 0 or P1 showed severe hearing loss with disordered stereocilia of the outer hair cells (OHCs) and impaired function of mechanoelectrical transduction of OHCs. In addition, we found that treatment with T3 at P0 or P1 resulted in the overproduction of Deiter-like cells. Compared with the control group, the transcription levels of Sox2 and notch pathway-related genes in the cochlea of the T3 group were significantly downregulated. Furthermore, Sox2-haploinsufficient mice treated with T3 not only showed excess numbers of Deiter-like cells but also a large number of ectopic outer pillar cells (OPCs). Our study provides new evidence for the dual roles of T3 in regulating both hair cells and supporting cell development, suggesting that it is possible to increase the reserve of supporting cells. [ABSTRACT FROM AUTHOR]

Purinergic ligand‐gated ion channel 7 receptor (P2X7 receptor) is an adenosine triphosphate (ATP)‐gated ion channel that is widely distributed on the surfaces of immune cells and tissues such as those in the liver, kidney, lung, intestine, and nervous system. Hepatocellular carcinoma (HCC) is one of the most common malignancies with increasing incidence and mortality. Although many treatments for liver cancer have been studied, the prognosis for liver cancer is still very poor. Therefore, new liver cancer treatments are urgently needed. P2X7 receptor activation can secrete proinflammatory factors through the P2X7 receptor‐NLRP3 signaling pathway, thereby affecting the progression of liver injury. The P2X7 receptor may be a target for growth inhibition of HCC cells and may affect the invasion and migration of HCC cells through the PI3K/AKT and AMPK signaling pathways. In recent years, P2X7 receptor antagonists or inhibitors have attracted widespread attention as therapeutic targets for hepatocellular carcinoma and liver injury. Therefore, this review covers the basic concepts of the P2X7 receptor and role of the P2X7 receptor in liver cancer and liver injury, providing new potential therapeutic targets for hepatocellular carcinoma and liver injury. [ABSTRACT FROM AUTHOR]

The two-sample Mendelian randomization (MR) study revealed a causal association of plasma proteins with osteoporosis (OP) and osteoarthritis (OA). Bone mineral density (BMD) is the gold standard for the clinical assessment of OP. Recent studies have shown that plasma proteins play an essential role in the regulation of bone development. However, the causal association of plasma proteins with BMD and OA remains unclear. We estimated the effects of 2889 plasma proteins on 2 BMD phenotypes and 6 OA phenotypes using two-sample MR analysis based on the genome-wide association study summary statistics. Then, we performed sensitivity analysis and reverse-direction MR analysis to evaluate the robustness of the MR analysis results, followed by gene ontology (GO) enrichment analysis and KEGG pathway analysis to explore the functional relevance of the identified plasma proteins. Overall, we observed a total of 257 protein-estimated heel BMD associations, 17 protein-total-body BMD associations, 2 protein-all-OA associations, and 2 protein-knee-OA associations at PFDR < 0.05. Reverse-direction MR analysis demonstrated that there was little evidence of the causal association of BMD and OA with plasma proteins. GO enrichment analysis and KEGG pathway analysis identified multiple pathways, which may be involved in the development of OP and OA. Our findings recognized plasma proteins that could be used to regulate changes in OP and OA, thus, providing new insights into protein-mediated mechanisms of bone development. [ABSTRACT FROM AUTHOR]

In this paper, well-defined Mg-Al LDHs nanosheets with high yield are prepared by the following sequential procedures: hydrothermal preparation of Mg-Al-CO 3 2 − LDHs, followed by expanding interlayer spacing by NH4NO3 treatment. The results show that the concentration of the layer expansion agent (NH4NO 3) and the exfoliation solvent play a key role in the exfoliation result. The basal spacing changed from 0.758 nm to the range of 0.758–0.895 nm when the concentration of NH4NO3 was increased up to 5 M, which infers that the increase of layer space is caused by the synergy of NH 4 + and NO 3 − . Among the three exfoliation solvents (formamide, N-methyl pyrrolidone and isopropyl alcohol), formamide showed the highest exfoliation effect. From the characteristic XRD patterns, the as-obtained colloidal aggregates of the exfoliated nanosheets do not show the characteristic diffractions of LDHs, while the typical peaks at 10.562 ∘ , 20.032 ∘ and 21.185 ∘ assigned to LDHs are observed after drying of the colloidal aggregates. Moreover, a transparent LDH film is also successfully prepared by coating colloidal aggregates of nanosheets on a glass substrate without using any adhesive. The stable film exhibits the strong adsorption ability of Methyl Orange. The success in obtaining well-defined Mg-Al LDHs nanosheets through the following sequential procedures was described: hydrothermal preparation of Mg-Al LDHs, expanded interlayer spacing by NH4NO3 treatment, and stable, transparent colloidal suspension with no solid residues after standing in formamide for 2 days. The product had strong adsorption ability. [ABSTRACT FROM AUTHOR]

Rose plants are widely cultivated as cut flowers worldwide and have economic value as sources of natural fragrance and flavoring. Rosa 'Crimson Glory', whose petals have a pleasant fragrance, is one of the most important cultivars of edible rose plants. Flower storage at low-temperature is widely applied in production to maintain quality; however, chilling results in a decrease in aromatic volatiles. To determine the molecular basis underlying the changes in aromatic volatile emissions, we investigated the changes in volatile compounds, DNA methylation patterns, and patterns of the transcriptome in response to chilling temperature. The results demonstrated that chilling roses substantially reduced aromatic volatile emissions. We found that these reductions were correlated with the changes in the methylation status of the promoters and genic regions of the genes involved in volatile biosynthesis. These changes mainly occurred for CHH (H = A, T, or C) which accounted for 51% of the total methylation. Furthermore, transcript levels of scent-related gene Germacrene D synthase (RhGDS), Nudix hydrolase 1 (RhNUDX1), and Phenylacetaldehyde reductase (RhPAR) of roses were strikingly depressed after 24 h at low-temperature and remained low-level after 24 h of recovery at 20 °C. Overall, our findings indicated that epigenetic regulation plays an important role in the chilling tolerance of roses and lays a foundation for practical significance in the production of edible roses. [ABSTRACT FROM AUTHOR]

Introduction: Parallel distributed processing theory (PDP theory) holds that all brain regions involved in conceptual representation perform a series of activities at the same time. However, the role of emotional experience information in concrete conceptual representation is still unknown. This study further explores whether the emotional experience will also affect the semantic processing of concrete concept representations. Methods: This study used the emotion priming paradigm and semantic judgment task to explore whether emotion priming impacts the processing of animal concepts with different emotional experiences through two experiments. In Experiments 1a and 1b, pleasant or disgusted faces were used as emotional priming stimuli to explore whether the explicit processing of emotions would affect the semantic processing of animal concepts. Experiments 2a and 2b used positive or negative scenery pictures as emotional priming stimuli to explore whether the implicit processing of emotions would affect the semantic processing of animal concepts. Results: The Experiment 1 results showed that the perception of faces promotes the processing of animal words, showing the "word-emotion congruence effect". Experiment 2a did not show the expected results, while Experiment 2b showed that the general negative perception of scenery pictures could significantly promote the processing of disgusted animal words. The results further proved the "word-emotion congruence effect" shown in the results of Experiment 1 from the perspective of implicit emotion processing. Combining the results of two experiments, it can be proven that emotional experience affects the semantic processing process of concrete concepts. Discussion: Both Experiment 1 and Experiment 2b of this study show the "word-emotion congruence effect". PDP theory believes that conceptual representation is represented by the activity patterns of billions of neurons distributed in many areas of the brain, and related semantic processing and sensory processing will occur simultaneously. The results of this experiment well support PDP theory. [ABSTRACT FROM AUTHOR]

Background: Photoperiod is an important environmental cue interacting with circadian clock pathway to optimize the local adaption and yield of crops. Quinoa (Chenopodium quinoa) in family Amaranthaceae has been known as superfood due to the nutritious elements. As quinoa was originated from the low-latitude Andes, most of the quinoa accessions are short-day type. Short-day type quinoa usually displays altered growth and yield status when introduced into higher latitude regions. Thus, deciphering the photoperiodic regulation on circadian clock pathway will help breed adaptable and high yielding quinoa cultivars. Results: In this study, we conducted RNA-seq analysis of the diurnally collected leaves of quinoa plants treated by short-day (SD) and long-day conditions (LD), respectively. We identified 19,818 (44% of global genes) rhythmic genes in quinoa using HAYSTACK analysis. We identified the putative circadian clock architecture and investigated the photoperiodic regulatory effects on the expression phase and amplitude of global rhythmic genes, core clock components and transcription factors. The global rhythmic transcripts were involved in time-of-day specific biological processes. A higher percentage of rhythmic genes had advanced phases and strengthened amplitudes when switched from LD to SD. The transcription factors of CO-like, DBB, EIL, ERF, NAC, TALE and WRKY families were sensitive to the day length changes. We speculated that those transcription factors may function as key mediators for the circadian clock output in quinoa. Besides, we identified 15 novel time-of-day specific motifs that may be key cis elements for rhythm-keeping in quinoa. Conclusions: Collectively, this study lays a foundation for understanding the circadian clock pathway and provides useful molecular resources for adaptable elites breeding in quinoa. [ABSTRACT FROM AUTHOR]

Recently, anisotropic particles have attracted attention because their unique topology, functions and wide applications. Here, a new type of acrylate copolymer microspheres (AcPMs) with anisotropic wrinkled surface was synthesized with conventional monomers through two‐step self‐assembly in one‐pot with regulating reaction media. First, butyl acrylate (BA), methyl methacrylate (MMA) and methacrylic acid (MAA) were copolymerized in EtOH/H2O solvent. Second, styrene (St) and BA were further copolymerized with increasing H2O proportion in solution, which enable AcPMs with emulsifiability. Its structure and morphology were characterized by FT‐IR, SEM, XRD and DLS. The surface‐wrinkled and anisotropic AcPMs was used as carrier to conjugate with AgNPs by in‐situ reduction of silver ions (Ag+), which afforded the AcPMs conjugating AgNPs (AgNPs@AcPMs), and it presented an excellent antibacterial activity against S. aureus and E. coli. Hence, AcPMs revealed strong potential in the biomedical fields. [ABSTRACT FROM AUTHOR]

This study aims to solve a one-dimensional multi-period cutting stock production replanning problem encountered in manufacturing industry cutting departments, considering pattern-setup costs. For operational purposes, long-term coordinated production and cutting plans are often created based on predicted demand. However, real demand may differ from predictions, and replanning may be warranted by changing conditions. A mixed-integer mathematical model was proposed by minimizing both the production cost and the cost of deviating from established plans. In addition, a hybrid dynamic programming-based heuristic was proposed. Extensive experiments were conducted on randomly generated instances. The influences on the solutions are analysed by varying the holding cost per unit and the deviation cost. Finally, the proposed heuristic is applied to enterprise practice, and the results indicate that the total cost can be expected to be reduced by 14.55% on average. [ABSTRACT FROM AUTHOR]

Biofilm-forming Staphylococcus aureus can easily accumulate on various food contact surfaces which induce cross-contamination and are difficult to eliminate in the food industry. This study aimed to evaluate the anti-biofilm effects of natural product biochanin A against S. aureus. Results showed that biochanin A effectively eradicated established S. aureus biofilms on different food-contact materials. Fluorescence microscopic analyses suggested that biochanin A disintegrated the established biofilms by dissociate extracellular polymeric substance (EPS) in matrix. In addition, biochanin A at the sub-MIC concentration also effectively inhibited the biofilm formation by regulating the expression of biofilm-related genes (icaA, srtA, eno) and suppressing the release of EPS in biofilm matrix. Molecular docking also demonstrated that biochanin A conducted strong interactions with biofilm-related proteins (Ica A, Sortase A, and Enolase). These findings demonstrated that biochanin A has the potential to be developed as a potent agent against S. aureus biofilm in food industries. Key points: • Anti-biofilm effect of biochanin A against S. aureus was revealed for the first time. • Biofilm of S. aureus on various food-contact surfaces were efficiently eradicated. • Biochanin A prevented S. aureus biofilm formation via reducing EPS production. [ABSTRACT FROM AUTHOR]

The blood–brain barrier is essential for maintaining the stability of the central nervous system and is also crucial for regulating drug metabolism, changes of blood–brain barrier's structure and function can influence how drugs are delivered to the brain. In high-altitude hypoxia, the central nervous system's function is drastically altered, which can cause disease and modify the metabolism of drugs in vivo. Changes in the structure and function of the blood–brain barrier and the transport of the drug across the blood-brain barrier under high-altitude hypoxia, are regulated by changes in brain microvascular endothelial cells, astrocytes, and pericytes, either regulated by drug metabolism factors such as drug transporters and drug-metabolizing enzymes. This article aims to review the effects of high-altitude hypoxia on the structure and function of the blood–brain barrier as well as the effects of changes in the blood-brain barrier on drug metabolism. We also hypothesized and explore the regulation and potential mechanisms of the blood-brain barrier and associated pathways, such as transcription factors, inflammatory factors, and nuclear receptors, in regulating drug transport under high-altitude hypoxia. [ABSTRACT FROM AUTHOR]

Butanol dehydrogenase (BDH) plays a significant role in the biosynthesis of butanol in bacteria by catalyzing butanal conversion to butanol at the expense of the NAD(P)H cofactor. BDH is an attractive enzyme for industrial application in butanol production; however, its molecular function remains largely uncharacterized. In this study, we found that Fusobacterium nucleatum YqdH (FnYqdH) converts aldehyde into alcohol by utilizing NAD(P)H, with broad substrate specificity toward aldehydes but not alcohols. An in vitro metal ion substitution experiment showed that FnYqdH has higher enzyme activity in the presence of Co2+. Crystal structures of FnYqdH, in its apo and complexed forms (with NAD and Co2+), were determined at 1.98 and 2.72 Å resolution, respectively. The crystal structure of apo- and cofactor-binding states of FnYqdH showed an open conformation between the nucleotide binding and catalytic domain. Key residues involved in the catalytic and cofactor-binding sites of FnYqdH were identified by mutagenesis and microscale thermophoresis assays. The structural conformation and preferred optimal metal ion of FnYqdH differed from that of TmBDH (homolog protein of FnYqdH). Overall, we proposed an alternative model for putative proton relay in FnYqdH, thereby providing better insight into the molecular function of BDH. [ABSTRACT FROM AUTHOR]

We designed and screened bifunctional catalysts with good oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) performance on bilayer MN 4 -O-MN 4 structures with bridge-bonded oxygen ligands. The ORR and OER catalytic activities of 225 bilayer MN 4 -O-MN 4 structures were explored in an accelerated manner by combining machine learning (ML) and density functional theory (DFT) calculations (DFT-ML). Based on the gradient boosted regression (GBR) algorithm, a series of efficient monofunctional and bifunctional electrocatalysts were successfully predicted with an average prediction error of only 0.04 V and 0.06 V for ORR and OER overpotential (η). ML successfully predicted that the overpotential of the monofunctional catalysts CoN 4 –O–RhN 4 (ORR) and RhN 4 –O–AgN 4 (OER) reached 0.34 V and 0.29 V, respectively; CoN 4 –O–AgN 4 was considered the best bifunctional catalyst due to its overpotential of η ORR = 0.35 V and η OER = 0.33 V on the bifunctional catalysts. Compared with DFT calculations, the DFT-ML accelerated calculation method resulted in a 9.4-fold improvement in catalyst screening speed. The performance prediction of 225 bilayer MN 4 -O-MN 4 structures was used to screen out the potential bifunctional catalysts, thus providing guidance for the experimental synthesis of better performing bridge-bonded oxygen ligand catalysts. [ABSTRACT FROM AUTHOR]

Cancer chemotherapy drugs are widely criticized for their serious side effects and low cure rate. Therefore, adjuvant therapy as a combination with chemotherapy administration is being accepted by many patients. However, unclear drug targets and mechanisms limit the application of adjuvant treatment. In this study, we confirmed TMEM16A is a key drug target for lung adenocarcinoma, and narirutin is an effective anti-lung adenocarcinoma natural product. Virtual screening and fluorescence experiments confirmed that narirutin inhibits the molecular target TMEM16A, which is specific high-expression in lung adenocarcinoma. Molecular dynamics simulations and electrophysiological experiments revealed the precise molecular mechanism of narirutin regulating TMEM16A. The anticancer effect of narirutin and its synergistic effect on cisplatin were explored by cell proliferation, migration, and apoptosis assays. The signaling pathways regulated by narirutin were analyzed by western blot. Tumor xenograft mice experiments demonstrated the synergistic anticancer effect of narirutin and cisplatin, and the side effects of high concentrations of cisplatin were almost eliminated. Pharmacokinetic experiments showed the biological safety of narirutin is satisfactory in vivo. Based on the significant anticancer effect and high biosafety, naringin has great potential as a functional food in the adjuvant treatment of lung cancer. [Display omitted] [ABSTRACT FROM AUTHOR]

The effects of removing non‐starch constituents like lipids, proteins, and β‐glucan on the in‐vitro digestibility of oat bran by steaming, microwaving, and hot air drying were investigated in this study. The results showed that the rapidly digestible starch (RDS) content of steaming oat bran decreased significantly (p < 0.05), and the glycemic index (e GI) was the lowest (66.11). The resistant starch (RS) content decreased, and in vitro digestibility, hydrolysis index, and eGI values of pretreated oat bran increased after removing the non‐starch constituents. Lipid removal increased in vitro digestibility the most, followed by β‐glucan and protein removal. The solubility of oat bran with removing protein increased significantly after steaming and microwaving treatment (p < 0.05). The relative crystallinity of oat bran decreased during steaming and increased after microwave and hot air drying. In conclusion, co‐existed components like proteins, fiber and lipids are helpful to a purpose of a low GI and slow starch digestion. Practical applications: In this study, high value‐added utilization of oat bran was realized, which solved the problems of resource waste and environmental pollution. Meanwhile, the steaming oat bran with intact structure is helpful for a purpose of low GI and slow starch digestion and is more beneficial to human health. [ABSTRACT FROM AUTHOR]

Starmerella bacillaris is often isolated from environments associated with grape and winemaking. S. bacillaris has many beneficial properties, including the ability to improve the flavor of wine, the production of beneficial metabolites, and the ability to biocontrol. S. bacillaris CC-PT4 (CGMCC No. 23573) was isolated from grape and can inhibit methicillin-resistant Staphylococcus aureus and adaptability to harsh environments. In this paper, the whole genome of S. bacillaris CC-PT4 was sequenced and bioinformatics analyses were performed. The S. bacillaris CC-PT4 genome was finally assembled into five scaffolds with a genome size of 9.45 Mb and a GC content of 39.5%. It was predicted that the strain contained 4150 protein-coding genes, of which two genes encoded killer toxin and one gene encoded lysostaphin. It also contains genes encoding F1F0-ATPases, Na(+)/H(+) antiporter, cation/H(+) antiporter, ATP-dependent bile acid permease, major facilitator superfamily (MFS) antiporters, and stress response protein, which help S. bacillaris CC-PT4 adapt to bile, acid, and other stressful environments. Proteins related to flocculation and adhesion have also been identified in the S. bacillaris CC-PT4 genome. Predicted by antiSMASH, two secondary metabolite biosynthesis gene clusters were found, and the synthesized metabolites may have antimicrobial effects. Furthermore, S. bacillaris CC-PT4 carried genes associated with pathogenicity and drug resistance. Overall, the whole genome sequencing and analysis of S. bacillaris CC-PT4 in this study provide valuable information for understanding the biological characteristics and further development of this strain. [ABSTRACT FROM AUTHOR]

The bike rebalancing problem is one of the major operational challenges in the urban bike-sharing system, which involves the redistribution of bikes among stations to prevent stations from being empty or overloaded. This paper investigates a new bike rebalancing problem, which considers the collection of broken bikes in the multi-depot system. The proposed problem can be classified as a two-commodity vehicle routing problem with pick-up and delivery. An integer programming model is formulated to find the optimal vehicle assignment and visiting sequences with the minimum total working time and fixed cost of vehicles. A hybrid heuristic algorithm integrating variable neighborhood search and dynamic programming is proposed to solve the problem. The computational results show that the proposed method can find 26 best solutions out of 36 instances, while the CPLEX obtains 16 best solutions. Impact of broken bikes collection and distribution of depots is examined. Comparison of different practical strategies indicates that the number of vehicles can be significantly reduced by allowing multiple visits to depots. Allowing vehicles to return to different depots can help reduce the total working time. [ABSTRACT FROM AUTHOR]

Machilus chuanchienensis is an ecological tree distributed in southwestern China. It has a significant valuation with making Hawk tea using its leaves, an ethnic traditional tea-like beverage with a long history in Chinese tea culture. The whole chloroplast (cp) genome is an ideal model for the phylogenetic study of Lauraceae because of its simple structure and highly conserved features. There have been numerous reports of complete cp genome sequences in Lauraceae, but little is known about M. chuanchienensis. Here, the next-generation sequencing (NGS) was used to sequence the M. chuanchienensis cp genome. Then, a comprehensive comparative genome analysis was performed. The results revealed that the M. chuanchienensis's cp genome measured 152,748 base pairs (bp) with a GC content of 39.15% and coded 126 genes annotated, including comprising eight ribosomal RNA (rRNA), 36 transporter RNA (tRNA), and 82 protein-coding genes. In addition, the cp genome presented a typical quadripartite structure comprising a large single-copy (LSC; 93,811) region, a small single-copy (SSC; 18,803) region, and the inverted repeats (IRs; 20,067) region and contained 92 simple sequence repeat (SSR) locus in total. Phylogenetic relationships of 37 species indicated that M. chuanchienensis was a sister to M. balansae, M. melanophylla, and M. minutiflora. Further research on this crucial species may benefit significantly from these findings. [ABSTRACT FROM AUTHOR]

Background: The brown adipose tissue (BAT) is a target for treating obesity. BAT losses thermogenic capacity and gains a “white adipose tissue-like” phenotype (“BAT whitening”) under thermoneutral environments, which is a potential factor causing a low curative effect in BAT-related obesity treatments. Circular RNAs (circRNAs) and long non-coding RNAs (lncRNAs) can act as competing endogenous RNAs (ceRNA) to mRNAs and function in various processes by sponging shared microRNAs (miRNAs). However, the roles of circRNA- and lncRNA-related ceRNA networks in regulating BAT whitening remain litter known. Results: In this study, BATs were collected from rabbits at day0 (D0), D15, D85, and 2 years (Y2). MiRNA-seq was performed to investigate miRNA changes during BAT whitening. Then, a combined analysis of circRNA-seq and whole-transcriptome sequencing was used for circRNA assembly and quantification during BAT whitening. Our data showed that 1187 miRNAs and 6204 circRNAs were expressed in the samples, and many of which were identified as significantly changed during BAT whitening. Target prediction showed that D0-selective miRNAs were significantly enriched in the Ras, MAPK, and PI3K-Akt signaling pathways, and Y2-selective miRNAs were predicted to be involved in cell proliferation. The cyclization of several circRNAs could form novel response elements of key thermogenesis miRNAs at the back-splicing junction (BSJ) sites, and in combination with a dual-luciferase reporter assay confirmed the binding between the BSJ site of novel_circ_0013792 and ocu-miR-378-5p. CircRNAs and lncRNAs have high cooperativity in sponging miRNAs during BAT whitening. Both circRNA-miRNA-mRNA and lncRNA-miRNA-mRNA triple networks were significantly involved in immune response-associated biological processes. The D15-selective circRNA might promote BAT whitening by increasing the expression of IDH2. The Y2-selective circRNA-related ceRNA network and lncRNA-related ceRNA network might regulate the formation of the WAT-like phenotype of BAT via MAPK and Ras signaling pathways, respectively. Conclusions: Our work systematically revealed ceRNA networks during BAT whitening in rabbits and might provide new insight into BAT-based obesity treatments. [ABSTRACT FROM AUTHOR]

Under the influence of COVID-19, online office and online education has ushered in a golden period of development. The teaching quality of online education has been a controversial issue. Our study takes online course teaching quality assessment as the starting point, explores the influencing factors of online course quality assessment with online courses as the research object, and analyzes the latest research proposal for an online course quality index. To make the online course quality assessment more intelligent, we propose an online course quality assessment method based on a fuzzy neural network. The method uses fuzzy rules as the baseline and adds a TSK perception mechanism to expand the perception domain of the fuzzy neural network and improve the course quality index prediction accuracy. At the input side of the fuzzy neural network, we preclassify the online course data into four parts, and each part of the data represents a different assessment domain. Due to the large data cost, we expanded the collective amount of data using data augmentation methods. In addition, we parse the structure of the fuzzy neural network hierarchy and introduce the construction and role of the TSK perception mechanism in the fuzzy rules. An optimal learning strategy is proposed in the fuzzy neural network training. Finally, in the experimental session, we verify the effectiveness of data augmentation and explore the distribution of course quality assessment weights. In the comparison of the model prediction results with the actual assessment results, our method achieves an excellent matching rate, which proves the high efficiency of our method in the online course quality assessment system. [ABSTRACT FROM AUTHOR]

NaMg 0.12 Nb 0.88 O 3 - x BiYbO 3 (abbreviated as NMN- x BY) (x = 0, 0.005, 0.01, 0.015, 0.02, 0.04 and 0.08) ceramics were fabricated by using a traditional solid-state reaction method. The crystal structure, morphology, relaxation behavior and energy storage properties of NMN- x BY) ceramics were systematically investigated. X-ray diffraction patterns (XRD) demonstrates that all samples present a single pseudo-cubic phase. It is also found that the grain sizes vary irregular with the BY content, and it can be reduced by the appropriate addition of BY content as indicated by scanning electron morphology. From the measured dielectric properties, it can be seen that all samples are transformed from normal ferroelectric (FEs) to relaxor ferroelectric (RFEs). The maximum relaxor diffuseness coefficient γ (∼1.78) is obtained for 0.98NMN-0.02BY ceramic. As x increases, the polarization-electric (P - E) hysteresis loops gradually become slimmer. The largest discharged energy density (∼0.998 J/cm3) and the maximum energy efficiency (∼70%) are obtained at 170 kV/cm for x = 0.04. Meanwhile, the discharge energy densities present relatively excellent temperature stability for x = 0.02 in the temperature range from 20 to 140 °C. This work demonstrates a wide potential application in enhancing the storage properties of lead-free ferroelectric materials by co-doping MgO and bismuth-based perovskites. [ABSTRACT FROM AUTHOR]

Background: Improving feed efficiency (FE) is one of the main objectives in broiler breeding. It is difficult to directly measure FE traits, and breeders hence have been trying to identify biomarkers for the indirect selection and improvement of FE traits. Metabolome is the "bridge" between genome and phenome. The metabolites may potentially account for more of the phenotypic variation and can suitably serve as biomarkers for selecting FE traits. This study aimed to identify plasma metabolite markers for selecting high-FE broilers. A total of 441 birds from Northeast Agricultural University broiler lines divergently selected for abdominal fat content were used to analyze plasma metabolome and estimate the genetic parameters of differentially expressed metabolites. Results: The results identified 124 differentially expressed plasma metabolites (P < 0.05) between the lean line (high-FE birds) and the fat line (low-FE birds). Among these differentially expressed plasma metabolites, 44 were found to have higher positive or negative genetic correlations with FE traits (|rg| ≥ 0.30). Of these 44 metabolites, 14 were found to display moderate to high heritability estimates (h2 ≥ 0.20). However, among the 14 metabolites, 4 metabolites whose physiological functions have not been reported were excluded. Ultimately, 10 metabolites were suggested to serve as the potential biomarkers for breeding the high-FE broilers. Based on the physiological functions of these metabolites, reducing inflammatory and improving immunity were proposed to improve FE and increase production efficiency. Conclusions: According to the pipeline for the selection of the metabolite markers established in this study, it was suggested that 10 metabolites including 7-ketocholesterol, dimethyl sulfone, epsilon-(gamma-glutamyl)-lysine, gamma-glutamyltyrosine, 2-oxoadipic acid, L-homoarginine, testosterone, adenosine 5'-monophosphate, adrenic acid, and calcitriol could be used as the potential biomarkers for breeding the "food-saving broilers". [ABSTRACT FROM AUTHOR]

HNF4α and HNF1α are core transcription factors involved in the development and progression of a variety of human diseases and drug metabolism. They play critical roles in maintaining the normal growth and function of multiple organs, mainly the liver, and in the metabolism of endogenous and exogenous substances. The twelve isoforms of HNF4α may exhibit different physiological functions, and HNF4α and HNF1α show varying or even opposing effects in different types of diseases, particularly cancer. Additionally, the regulation of CYP450, phase II drug-metabolizing enzymes, and drug transporters is affected by several factors. This article aims to review the role of HNF4α and HNF1α in human diseases and drug metabolism, including their structures and physiological functions, affected diseases, regulated drug metabolism genes, influencing factors, and related mechanisms. We also propose a transcriptional regulatory network of HNF4α and HNF1α that regulates the expression of target genes related to disease and drug metabolism. [ABSTRACT FROM AUTHOR]

The outer protoplanetary discs (PPDs) can be subject to the magnetorotational instability (MRI) and the vertical shear instability (VSI). While both processes can drive turbulence in the disc, existing numerical simulations have studied them separately. In this paper, we conduct global 3D non-ideal magnetohydrodynamic (MHD) simulations for outer PPDs, with ambipolar diffusion and instantaneous cooling, and hence conductive to both instabilities. Given the range of ambipolar Elsässer numbers (Am) explored, it is found that the VSI turbulence dominates over the MRI when ambipolar diffusion is strong (Am  = 0.1); the VSI and MRI can co-exist for Am  = 1; and the VSI is overwhelmed by the MRI when ambipolar diffusion is weak (Am  = 10). Angular momentum transport process is primarily driven by MHD winds, while viscous accretion due to MRI and/or VSI turbulence makes a moderate contribution in most cases. Spontaneous magnetic flux concentration and formation of annular substructures remain robust in strong ambipolar diffusion-dominated discs (Am ≤ 1) with the presence of the VSI. Ambipolar diffusion is the major contributor to the magnetic flux concentration phenomenon rather than advection. [ABSTRACT FROM AUTHOR]

We investigate the dynamics of measurement-induced nonlocality (MIN) with two spins in an ideal single-mode optical cavity. The two-spin entangled states are generated firstly under the photon coherent state assumption by means of the variational method. The time evolution of MIN is then examined based on the derived analytical expressions of the reduced density operator of two spins. It is shown that MIN remains in its upper bound all the time and independent of the average photon number of the coherent field when the two spins are initially in the singlet state, which just is the eigenstate of our model. However, for the initial general two-spin entangled states of antiparallel and parallel spin polarizations, MIN strongly depends on the average photon number and initial angle parameters. We provide a detailed analysis of MIN dynamics by the numerical calculation, and reveal some significant results. [ABSTRACT FROM AUTHOR]

Background: The significance of S100A8/A9 and S100A12 in anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) has not been clarified. This study was dedicated to exploring the potential pathogenic roles of S100A8/A9 and S100A12 in patients with myeloperoxidase (MPO)-ANCA-positive vasculitis. Methods: Serum and urine concentrations of S100A8/A9 and S100A12 of forty-two AAV patients were evaluated. The influence of S100A8/A9 and S100A12 on the chemotaxis, the apoptosis, the release of IL-1β, the complement activation, the respiratory burst, as well as the neutrophil extracellular traps (NETs) formation of MPO-ANCA-activated neutrophils was investigated. Results: The serum and urine S100A8/A9 and S100A12 of active MPO-AAV significantly increased (compared with inactive AAV and healthy controls, p < 0.001) and were correlated with the severity of the disease. In vitro study showed that S100A8/A9 and S100A12 activated the p38 MAPK/NF-κB p65 pathway, increased the chemotaxis index (CI) and the release of IL-1β, extended the life span, and enhanced the complement activation ability of MPO-ANCA-activated neutrophils. The Blockade of TLR4 and RAGE inhibited the effects of S100A8/A9 and S100A12. All above-mentioned effects of S100A8/A9 and S100A12 were ROS-independent because neither S100A8/A9 nor S100A12 enhanced the ROS formation and NETs formation of MPO-ANCA-activated neutrophils. Conclusion: S100A8/A9 and S100A12 serve as markers for assessing the disease severity, and they may also play a role in MPO-AAV pathogenesis. [ABSTRACT FROM AUTHOR]

Objective: Infiltration of tumor-associated macrophages is closely linked to the malignant development of human cancers. This research studies the function of C-C motif chemokine ligand 7 (CCL7) in the macrophage accumulation in lung adenocarcinoma (LUAD) and the underpinning mechanism.Methods: The expression profile of CCL7 in LUAD and its correlations with patient's prognosis and macrophage infiltration were predicted via bioinformatics systems. Artificial up- or downregulation of CCL7 was induced in LUAD cells to explore its function in the mobility, EMT of cancer cells, and migration of M2 macrophages. Cancer cells were implanted in NOD/SCID mice to induce xenograft tumors. The CCL7-related transcription factors or factors were predicted by bioinformatic tools, and the molecular interactions were confirmed by immunoprecipitation or luciferase assays.Results: CCL7 was highly expressed in LUAD and linked to increased TAM infiltration. Knockdown of CCL7 suppressed the chemotaxis and M2 skewing of macrophages, and it blocked the EMT and mobility of LUAD cells. CCL7 downregulation also suppressed macrophage infiltration in xenograft tumors in mice. Spi-1 proto-oncogene (SPI1) was confirmed as an upstream factor activating CCL7 transcription, and LINC01094 was found to bind to SPI1 to promote its nuclear translocation. Upregulation of SPI1 restored the chemotactic migration and M2 polarization of macrophages in LUAD cells.Conclusion: This paper reveals that LINC01094 binds to SPI1 to promote its nuclear translocation, which further activates CCL7 transcription by binding to its promoter, leading to M2 macrophage accumulation and dissemination of tumor cells. [ABSTRACT FROM AUTHOR]

Background and Objectives Care planning can protect against or offset potential stressors in the caregiving stage and mitigate their detrimental effects. This study aimed to translate, adapt, and validate 2 short forms of the multidimensional, theory-guided scale measuring preparation for future care needs (PFCN) among Chinese older adults in Hong Kong. Research Design and Methods Data were derived from a cross-sectional survey of 862 community-dwelling individuals aged 60 years and older. Exploratory factor analysis (EFA) and confirmatory factor analysis (CFA) were conducted to assess the structural validity of the scales. Criterion-related validity, known-groups validity, and internal consistency were also examined. Results EFA yielded a 14-item 4-factor (awareness, avoidance, decision making, and concrete planning) model, which was supported by CFA and explained 68.9% of the total variance. CFA also supported the structural validity of the 5-item scale. Criterion-related validity of the 2 scales was supported by their significant and positive correlations with domain-specific planning behaviors for retirement. Known-groups validity of the 2 scales was demonstrated by significant differences in scores between male and female older adults and scores between different educational levels and socioeconomic status. Cronbach's alphas for the internal consistency of the 14-item and 5-item scales were 0.889 and 0.774, respectively. Discussion and Implications PFCN scales enable researchers and service practitioners to accurately understand and assess older adults' processes and efforts in care planning, facilitate the identification of individuals at risk from inadequate planning, and inform the development of interventions to enhance care preparation in target domains. [ABSTRACT FROM AUTHOR]

Background: Programmed cell death receptor‐1 (PD‐1) monotherapy is a standard treatment for advanced cutaneous melanoma, but its efficacy and toxicity are defined in white populations and remain poorly characterized in other ethnic groups, such as East Asian, Hispanic and African. Objectives: To determine the efficacy and toxicity of PD‐1 monotherapy in different ethnic groups. Methods: Clinical data for patients with unresectable or advanced melanoma treated with anti‐PD‐1 monotherapy between 2009 and 2019 were collected retrospectively from five independent institutions in the USA, Australia and China. Tumour response, survival and immune‐related adverse events (irAEs) were compared by ethnicity (white vs. East Asian/Hispanic/African) across different melanoma subtypes: nonacral cutaneous (NAC)/unknown primary (UP) and acral/mucosal/uveal. Results: In total, 1135 patients were included. White patients had significantly higher objective response rate (ORR) [54%, 95% confidence interval (CI) 50–57% vs. 20%, 95% CI 13–28%; adjusted P < 0·001] and longer progression‐free survival (14·2 months, 95% CI 10·7–20·3 vs. 5·4 months, 95% CI 4·5–7·0; adjusted P < 0·001) than East Asian, Hispanic and African patients in the NAC and UP subtypes. White ethnicity remained independently associated with a higher ORR (odds ratio 4·10, 95% CI 2·48–6·81; adjusted P < 0·001) and longer PFS (hazard ratio 0·58, 95% CI 0·46–0·74; adjusted P < 0·001) in multivariate analyses after adjustment for age, sex, primary anatomical location, metastasis stage, baseline lactate dehydrogenase level, mutational status and prior systemic treatment. White and East Asian/Hispanic/African patients shared similar ORR and progression‐free survival in acral/mucosal/uveal melanomas. Similar melanoma‐subtype‐specific ethnic discrepancies were observed in complete response rate and overall survival. White patients had higher rates of gastrointestinal irAEs but lower rates of endocrine, liver and other rare types of irAEs. These differences in irAEs by ethnicity were not attributable to varying melanoma subtypes. Conclusions: Ethnic discrepancy in clinical benefit is specific to melanoma subtype, and East Asian, Hispanic and African patients with NAC and UP melanomas have poorer clinical benefits than previously recognized. The ethnic discrepancy in toxicity observed across different melanoma subtypes warrants an ethnicity‐based irAE surveillance strategy. More research is needed to elucidate the molecular and immunological determinants of these differences. What is already known about this topic?There is a great difference in response to immunotherapy between different subtypes of melanoma (cutaneous, mucosal, acral and uveal) in patients with advanced disease. What does this study add?Our data show for the first time that there are differences between different ethnic groups in terms of both response and toxicity to immunotherapy beyond the well‐appreciated discrepancies due to melanoma subtype. [ABSTRACT FROM AUTHOR]

According to the requirements of carbon-neutral development, this study explores the comparison and new discussion of replacing nitrogen with carbon dioxide in the conventional two-phase microfluid flow. Thus, carbon dioxide application in various fields can be more precise and convenient. This research uses an artificially continuously tapering micro model to mimic the natural rock channel in low permeability formation, where the liquid imbibition process is entirely under surface tension-dominant. The tested capillary number decreased to 8.49 × 10−6, and the thinnest observed liquid film was reduced to 2 μm. The comparison results in two gas groups (nitrogen and carbon dioxide) show that CO2 gas fluid in microscopic porous media would have more tendency to snap off and leave fewer residual bubbles blocked between the constrictions. However, the N2 gas fluid forms smaller isolated gas bubbles after snap-off. By combining the experimental data and numerical output with the theoretical evolution equation by Beresnev and Deng and by Quevedo Tiznado et al., the results of interface radius, temporal capillary pressure, and velocity profiles for axisymmetric and continuously tapering models are presented and validated. Those findings create a paradigm for future studies of the evolution of microscopic multiphase fluid and enhance a deeper understanding of geological underground fluid properties for greenhouse gas storage and utilization in low permeability formations. [ABSTRACT FROM AUTHOR]

In a variety of skeletal structures of delayed fluorescence molecular materials, the donor–acceptor–donor (D–A–D) type has been widely considered for improving the efficiency of the reverse intersystem crossing (RISC) process. Herein, three new D–A–D molecules (PTZ-MPS, TPA-MPS and PCz-MPS) bearing 9,9-dimethylthioxanthene-S,S-sulfur dioxide (MPS) as the electron acceptor group are designed and investigated using theoretical calculations. PTZ-MPS shows the feature of the high-lying reverse intersystem crossing process, which is conducive to improving the exciton utilization of organic light-emitting diodes (OLEDs). PTZ-MPS has a much smaller singlet–triplet energy splitting (ΔES1T3 = 0.03 eV) than TPA-MPS (ΔES1T3 = 0.32 eV) and PCz-MPS (ΔES1T3 = 0.59 eV). However, it has a much larger spin–orbital coupling (SOC) strength (〈S1‖ĤSOC‖T3〉 = 1.013 cm−1) than TPA-MPS (〈S1‖ĤSOC‖T3〉 = 0.311 cm−1) and PCz-MPS (〈S1‖ĤSOC‖T3〉 = 0.354 cm−1), which makes it easy to induce a sufficient RISC from the Tn state to the S1 state. The ΔEST and SOC are the two most important factors in determining TADF molecules. Therefore, PTZ-MPS is expected to be a potential high-lying excited state delayed fluorescence material candidate, and our work demonstrates that high-performance TADF materials can also be obtained successfully by designing rational molecules. [ABSTRACT FROM AUTHOR]

The existence of antibiotics in the environment can trigger a number of issues by fostering the widespread development of antimicrobial resistance. Currently, the most popular techniques for removing antibiotic pollutants from water include physical adsorption, flocculation, and chemical oxidation, however, these processes usually leave a significant quantity of chemical reagents and polymer electrolytes in the water, which can lead to difficulty post-treating unmanageable deposits. Furthermore, though cost-effectiveness, efficiency, reaction conditions, and nontoxicity during the degradation of antibiotics are hurdles to overcome, a variety of photocatalysts can be used to degrade pollutant residuals, allowing for a number of potential solutions to these issues. Thus, the urgent need for effective and rapid processes for photocatalytic degradation leads to an increased interest in finding more sustainable catalysts for antibiotic degradation. In this review, we provide an overview of the removal of pharmaceutical antibiotics through photocatalysis, and detail recent progress using different nanostructure-based photocatalysts. We also review the possible sources of antibiotic pollutants released through the ecological chain and the consequences and damages caused by antibiotics in wastewater on the environment and human health. The fundamental dynamic processes of nanomaterials and the degradation mechanisms of antibiotics are then discussed, and recent studies regarding different photocatalytic materials for the degradation of some typical and commonly used antibiotics are comprehensively summarized. Finally, major challenges and future opportunities for the photocatalytic degradation of commonly used antibiotics are highlighted. [ABSTRACT FROM AUTHOR]

Breast cancer is the most common form of cancer in women in need for better treatment. Sophoridine, a plant-derived quinoxidine alkaloid, has been shown to exhibit pharmacological effects on several diseases including lung and gastric cancers. To further extend these beneficial effects we have explored the role of sophoridine in breast cancer progression. Our results show that sophoridine suppressed the proliferation of breast cancer cells and inhibited the migration as well as invasion. In addition, sophoridine inhibited the angiogenesis of breast cancer cells. Mechanically, our data confirmed that sophoridine suppressed the vascular endothelial growth factor receptor 2/extracellular signal-regulated kinase 2 pathway, and therefore suppressed breast cancer progression. We therefore suggest that sophoridine could serve as a promising drug for the treatment of breast cancer. [ABSTRACT FROM AUTHOR]

• The coupling relationship between trajectory and battery is considered. • Trajectory-battery integrated design is proposed for spacecraft. • Electric Pump-Fed Engines are applied to low-Earth orbital maneuvers. • Advantages of the electric pump system in the structural mass are revealed. This paper proposes a trajectory-battery integrated design of the electric pump-fed (EPF) engine and demonstrates its application feasibility to orbital maneuvers in low-Earth orbit (LEO). Based on the mass model that comprises the main components of the engine, the advantages of the electric pump system in terms of the structural mass, addition of total impulse and change in velocity that the engine can provide are demonstrated compared with those of the traditional pressure-gas system. The performances of four typical battery cells are assessed to select the suitable cell for the application cases. Besides, the applications of the trajectory-battery integrated design to the specific orbital maneuvers are investigated. Impulsive and continuous control strategies are considered based on the EPF engine limited by the light/umbra condition and charge time. The optimal transfer trajectories considering the charging processes of the battery involved in orbital maneuvers are demonstrated. For the impulsive thrust, the limitation of the battery performance with regard to the effectiveness of the Hohmann transfer is given. For the continuous thrust, the orbital control problem has been turned into an optimization problem and the optimal fuel consumption is obtained using the combinatorial numerical optimization. The simulations confirm the superiority of the EPF engine based on its significant reduction of the structural mass and validate the application feasibility to orbital maneuvers in LEO. [ABSTRACT FROM AUTHOR]

The sintered NdFeB is widely used in many fields due to its excellent magnetic properties. However, the sintered NdFeB is characterized by its high hardness and brittleness. Therefore, its application has been limited. In order to improve its machining properties by electrical discharge machining (EDM), the deionized water is used as the dielectric medium, and then the sintered NdFeB is processed by the electrical discharge machining milling (EDM-M). The machining properties are studied by the comparative experiments. Changing the set value of the current, get the material removal rate (MRR), the surface roughness (SR) and the tool wear rate (TWR) of the die-sinking EDM in the kerosene-based medium (KEDM), the EDM-M in the kerosene-based medium (KEDM-M), the die-sinking EDM in the deionized water (DEDM), and the EDM-M in the deionized water (DEDM-M), respectively. By the scanning electron microscope (SEM), the influences of current on the material removal mechanism and surface formation mechanism of the DEDM-M are analyzed. Meanwhile, the characteristic of its surface micro-morphology influenced by current is analyzed. Combined SEM with energy-dispersive spectroscopy (EDS), the cross-section of the sintered NdFeB by the DEDM-M is analyzed. It is revealed by the comparative experiments that, for the sintered NdFeB by the DEDM-M, the interval of the machining parameter is wide; the value of SR is lowest obviously under low current set value; the variations of its MRR, SR, and TWR are regular; the controllability is good. The regression equations for the machining properties of the DEDM-M are obtained and its analysis of variance (ANOVA) is good. The analysis on the SEM of its machined surface indicates that the material removal and surface formation of the DEDM-M are effected synthetically by fusion and stress. The change of current leads to the difference in surface micro-morphology by fusion removal, globule, microvoid, microcrack, and surface micro-morphology by stress removal, and then leads to differences in MRR and SR, respectively. The results of analysis on the cross-section reveal that there are four categories from the surface to the matrix. They are recast layer after fusion with plenty of rare earth elements and minor oxygen which is amorphous and sheet, recast layer after fusion with plenty of iron element and minor rare earth elements which is strip and granular, micro-morphology by stress, and heat affected layer that is composed of large grains with thin grain boundaries. [ABSTRACT FROM AUTHOR]

Intestinal diseases in ruminants are frequent and susceptible to invasion by exogenous substances, and the intestinal mucosal barrier is the first line of defence of the body's immune defence. At present, the study on the structure of intestinal mucosal immune barrier in alpaca is incomplete. Therefore, the alpaca intestines were studied to show the distribution characteristics of intestinal mucosal barrier structure and cells associated with immune system using histology, histochemistry, and immunohistochemistry. The results showed that the intestinal tract of alpaca was composed of mucosa, submucosa, muscularis, and serosa. Intraepithelial lymphocytes were distributed in mucosal epithelium and glands of the large intestine. Mast cells were distributed in each segment of the intestine, mainly in the intestinal lamina propria, intestinal glands, and duodenal glands around, as well as in the muscularis, and the particles of cytoplasm were obvious. Acidic goblet cells were mainly distributed in the ileal mucosal epithelium and ileal intestinal glands, while sialomucins were mainly expressed in the colon. The cells associated with the immune system in the intestinal mucosa of alpaca play an important role in protecting against foreign microbial invasion and infection, and this result provides a theoretical basis for revealing the occurrence of gastrointestinal diseases in alpaca. [ABSTRACT FROM AUTHOR]

Introduction: As the frequency of infectious diseases rises, it's more important than ever to pay attention to the competency level of front-line nurses as the primary force in front-line rescue, which has an impact on the quality of anti-epidemic response. This paper aims to construct the competency evaluation index system for front-line nurses during the outbreak of major infectious diseases. Materials and methods: This study combined literature review, critical incident technique interviews, and semi-structured in-depth interviews, as well as two rounds of Delphi expert correspondence, to construct a competence evaluation index system for front-line nurses during the outbreak of major infectious diseases. The study used purposive sampling to select 26 experts from 11 provinces and cities across China to conduct two rounds of Delphi expert consultation, and the indicators were selected based on the mean importance score > 3.5 and the coefficient of variation < 0.25, and the weights of the indicators were calculated by the Analytic Hierarchy Process. The effective recovery rates of the two rounds of correspondence questionnaires were 93.1% and 96%. Results: The effective recovery rates of the two rounds of correspondence questionnaires were 93.1% and 96%, the authority coefficients of experts were 0.96 and 0.98, the Kendall's coordination coefficients of the first, second, and third level indexes were 0.281, 0.132, and 0.285 (P < 0.001), 0.259, 0.158, and 0.415 (P < 0.001). The final index system includes 4 primary indicators (Knowledge System of Infectious Diseases, Nursing Skills for Infectious Diseases, Related Professional Abilities for Infectious Diseases, and Comprehensive Quality), 10 secondary indicators, and 64 tertiary indicators. Conclusion: The competency evaluation index system of front-line nurses during the outbreak of major infectious diseases is scientific, reasonable, and practical, which can provide a scientific basis for nursing managers to accurately understand, describe, analyze, and evaluate the competence level of nursing staff and scientifically implement the allocation of human resources in the future, as well as serve as a content framework for subsequent training programs. [ABSTRACT FROM AUTHOR]

The reconfiguration of a constellation with several faulty satellites concerns performance improvements in multiple fields, which can be regarded as a multiobjective optimization (MOO) problem. In the optimization design, it is inevitable to evaluate the constellation performance and providing the reconfiguration strategy thousands of times is time-consuming. To decrease the high computational expense, this paper proposes an accurate and efficient MOO method based on the kriging surrogate model, termed as the surrogate-based MOO (SBMOO) algorithm. A new hybrid refinement method is presented to select infilling samples for updating the surrogate model. Different from the orbital phasing maneuver, the low-thrust reconfiguration strategy is implemented to optimize the transfer trajectory with low fuel consumption, by changing the orbital inclination and right ascension of the ascending node. With the tradeoff between the constellation performance and the uniformity of fuel consumption, the MOO problem of constellation reconfiguration can be investigated and settled by the proposed SBMOO algorithm. The simulations confirm that the preferable constellation reconfigurations are achieved with a low computational expense for optimization and a low fuel cost for orbital transfer. [ABSTRACT FROM AUTHOR]

Cropland soil erosion is primarily caused by agricultural production and indirectly altered by consumption patterns. A well-developed grain trade system transfers grain products to distant locations, away from their place of origin where soil erosion may have occurred. Evidence-based policies to control soil erosion can be developed by holding consumers responsible for the soil erosion caused by their consumption. However, there have been few systematic studies on cropland soil erosion that is embodied in grain trade. In this study, we estimated the soil erosion intensity (SEI) of grain production in each province of China from 1990 to 2020 by combining the Revised Universal Soil Loss Equation and socio-economic statistics. We also constructed an interprovincial grain trade network using linear programming techniques and analyzed the cropland soil erosion associated with the interprovincial grain trade. The results showed a rise in national cropland soil erosion attributed to grain production from 5.36×108 t in 1990 to 7.56×108 t in 2020. In particular, those embodied in interprovincial grain trade increased from 0.94×108 t in 1990 to 3.52×108 t in 2020. Nationally, about 10.72% of soil erosion exports in 2020 was from provinces with lower-than-average SEI, compared to 18.42% in 1990, indicating that current patterns of grain production and consumption are unfavorable for erosion control. Accordingly, measures have been proposed to build a soil erosion control system that includes consumers, such as changing farming practices, optimizing agricultural layout, promoting technology export and establishing a provincial compensation mechanism. [Display omitted] • Trade was prominent and insufficiently explored driver for cropland soil erosion. • RUSLE and grain trade network were utilized to estimate embodied soil erosion. • Mismatch between production and consumption increased embodied soil erosion. • Soil erosion burden mainly shifted from south with high SEI to north with low SEI. • Current provincial grain trade patterns were not conducive to soil erosion control. [ABSTRACT FROM AUTHOR]

• We study the dependence of the size and stability, for an ideal magnetic skyrmion bags in a cylindrical ultrathin nanodot, on the varying applied magnetic field. • By adjusting the magnetic parameters of the materials, the stability of skyrmion bags can be significantly enhanced. • Our results will provide valuable insights for the experimental development of magnetic multilayers tailored for devices based on skyrmion bags. Size and stability of magnetic skyrmion bags in a cylindrical ultrathin nanodot under the influence of an applied magnetic field are investigated. Micromagnetic calculations reveal unique characteristics in the behavior of skyrmion bags across various materials. By adjusting the magnetic parameters of the materials, the stability of skyrmion bags can be significantly enhanced. We believe that our results will provide valuable insights for the experimental development of magnetic multilayers tailored for devices based on skyrmion bags. [ABSTRACT FROM AUTHOR]

The construction of heterojunction structures is pivotal in enhancing carrier separation and photocatalytic activity. Hence, AgI/PCN-224 (APN-x composites) Z-scheme heterojunctions were successfully synthesised using an in-situ precipitation method. The direction of electron migration and the band structures of APN-x were comprehensively investigated using a combination of experimental approaches and density functional theory (DFT) calculations. Owing to the extensive visible-light absorption capabilities of PCN-224 and the high efficiency of photogenerated carrier separation in the Z-scheme heterojunction, the optimally developed APN-3 photocatalyst demonstrated exceptional photodegradation efficiency for tetracycline hydrochloride (TCH). The removal rate of TCH by APN-3 within 120 min reached 91.56%, approximately 4.64 times higher than that achieved by pure AgI. The photocatalytic degradation mechanism was explored using free radical trapping experiments, electron spin resonance (ESR) technology and band structure analysis. Degradation pathways and intermediate products were investigated using liquid chromatography–tandem mass spectrometry (LC–MS). [Display omitted] [ABSTRACT FROM AUTHOR]