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This book provides comprehensive knowledge of the methods of detection and identification of phyllosphere microbial pathogens and the management of different kinds of diseases caused by them in various crops. Interactions between pathogens and host plants result in the induction of defense responses expressed via molecular signals, from initiation of infection to systemic progression of pathogen invasion in susceptible plants and contrasting signals in resistant plants, leading to inhibition of pathogen development through activation of preinfectional and postinfectional defense responses. These are critically discussed.The author describes the intricate and complex competitive activities of the pathogens and host plants in a molecular warfare that the host plant must win to break the link in the pathogens'life cycle, allowing the development of disease management strategies based on the principles of exclusion, eradication, and immunization. Integration of strategies concerning the development of cultivars resistant to pathogens through breeding and biotechnological techniques, application of biotic and abiotic inducers of resistance to pathogens, and use of disease-free seeds and propagules that are complementary to each other along with effective cultural practices are emphasized.This book presents information gathered through an extensive literature search to help researchers and graduate students in agricultural sciences identify research gaps and successfully complete their research projects.

Viruses that infect plants are responsible for reduction in both yield and quality of crops around the world, and are thus of great economic importance. This has provided the impetus for the extensive research into the molecular and cellular biology of these pathogens and into their interaction with their plant hosts and their vectors. However interest in plant viruses extends beyond their ability to damage crops. Many plant viruses, for example tobacco mosaic virus, have been used as model systems to provide basic understanding of how viruses express genes and replicate. Others permitted the elucidation of the processes underlying RNA silencing, now recognised as a core epigenetic mechanism underpinning numerous areas of biology. This book attests to the huge diversity of research in plant molecular virology. Written by world authorities in the field, the book opens with two chapters on the translation and replication of viral RNA. Following chapters cover topics such as viral movement within and between plants, plant responses to viral infection, antiviral control measures, virus evolution, and newly emerging plant viruses. To close there are two chapters on biotechnological applications of plant viruses. Throughout the book the focus is on the most recent, cutting-edge research, making this book essential reading for everyone, from researchers and scholars to students, working with plant viruses.

Studies on the phenomenon of plant pathogenesis (disease development) have been useful to have a deep insight into the interactions between host plant and the pathogen. Depending on the levels of susceptibility (compatibility) or resistance (incompatibility) of the host plant and virulence of the pathogen, disease development may progress, either leading to symptom expression or result in the suppression of pathogen proliferation. Molecular techniques have been applied to elucidate the nature of interactions between the gene products of the plant and pathogen at cellular and molecular levels. Successful evasion of host's surveillance system and subsequent activities of metabolites of the pathogen (enzymes and toxins) encoded by pathogen genes counteracting the effects of various defense-related antimicrobial compounds present already or produced by the host plants, after initiation of infection have been critically studied by applying various molecular techniques. In addition to studying various phases of disease development in individual plants, molecular methods have been demonstrated to be effective, in gathering data on various aspects of epidemiology under natural conditions where the interaction of pathogen with populations of plants is influenced significantly by the environmental conditions existing in different ecosystems. This volume focuses on the possibility of applying the knowledge on pathogenesis and molecular epidemiology to determine the vulnerable stages in the life cycles of the pathogens that can be disrupted to achieve more effective disease control.

Studies on molecular biology of pathogens, infection process and disease resistance, have provided information essentially required to understand the vulnerable stages at which the pathogens can be tackled effectively and to adopt novel strategies to incorporate disease resistance genes from diverse sources and /or to induce resistance of cultivars with desirable agronomic attributes using biotic or abiotic agents. The nature of interaction between the gene products of the pathogen and plant appears to determine the outcome of the interaction resulting in either disease progression or suppression. Transgenic plants with engineered genes show promise for effective exploitation of this approach for practical application. Research efforts during the recent years to sequence the whole genomes of the pathogens and plants may lead to development of better ways of manipulating disease resistance mechanisms enabling the grower to achieve higher production levels and the consumer to enjoy safer food and agricultural products. Experimental protocols included in appropriate chapters will be useful for researchers and graduate students.

POSTHARVEST PATHOGENS AND DISEASE MANAGEMENT Postharvest diseases caused by microbial pathogens account for millions of dollars in losses of both durable and perishable produce products every year. Moreover, with consumers increasingly demanding minimally processed vegetables and fruits--which can be invaded by human pathogens--there is an imperative need for suitable protective measures to provide pathogen-free commodities that are free from, or contain only acceptable levels of, chemical residues. Providing details of both conventional and modern molecular techniques applicable for the detection, identification, and differentiation of field and storage microbial pathogens, Postharvest Pathogens and Disease Management: • Discusses diseases of both durables and perishables during transit and storage • Provides a basic understanding of the effects of handling and storage practices as well as field conditions and product susceptibility on the development of postharvest diseases • Reveals, as a cautionary note, the potential hazards of mycotoxins with carcinogenic properties that can contaminate fruits and vegetables • Contains detailed information derived from elucidative evidence and disease data in order to explain the infection process and subsequent stages of disease development • Helps readers to avoid conditions that favor disease incidence and spread • Includes real life examples of disease management strategies to help readers develop effective disease management systems suitable for different ecosystems • Emphasizes the importance of integrating several different effective methods in tandem, including the development of cultivars with resistance to postharvest diseases; the selection of suitable analytical methods; and the effective use of biocontrol agents and chemicals • Presents protocols for numerous techniques and basic methods, making the book a distinctive and highly useful teaching and research tool Postharvest Pathogens and Disease Management offers readers insight into the principles and methods of avoiding and managing postharvest diseases of fruit and vegetable products in an efficient, economical, and environmentally feasible manner, allowing producers to sell safer, higher-quality produce to the public and prevent the losses associated with postharvest disease.