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The interaction between dislocations and nano-precipitates during deformation directly influences hardening response of precipitation-strengthening metals such as Al-Mg-Si alloys. However, how coherent and semi-coherent nano-precipitates accommodate external deformation applied to an Al alloy remains to be elucidated. In-situ tensile experiments in a transmission electron microscope (TEM) were conducted to study the dynamic process of dislocations cutting through coherent needle-like β″ precipitates with diameters of 3∼8 nm. Comprehensive investigations using in-situ, ex-situ TEM and atom probe tomography uncovered that β″ precipitates were firstly sheared into small fragments, and then the rotation of the fragments, via sliding along precipitate/matrix interfaces, destroyed their initially coherent interface with the Al matrix. In contrast, semi-coherent βʹ precipitates with sizes similar to β″ were more difficult to be fragmented and accumulation of dislocations at the interface increased interface misfit between βʹ and the Al matrix. Consequently, βʹ precipitates could basically maintain their needle-like shape after the tensile deformation. This research gains new insights into the interaction between nano-precipitates and dislocations. [Display omitted] [ABSTRACT FROM AUTHOR]