1. Rapid, Portable Pathogen Detection with Multiplexed SERS-based Nanosensors.
- Author
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Kearns, Hayleigh, Jamieson, Lauren E., Graham, Duncan, Faulds, Karen, and Rathmell, Cicely
- Subjects
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NANOSENSORS , *DRUG resistance in bacteria , *PATHOGENIC microorganisms , *METAL nanoparticles , *BACTERIAL diseases - Abstract
Antibiotic resistance rates are rising for many common pathogens, putting added pressure on healthcare providers to rapidly, and accurately, diagnose the cause of bacterial infections. New diagnostic methods must be sensitive, selective, and capable of multiplexed detection, as well as rapid, dependable, and portable enough to operate in a variety of settings. A new application of surface-enhanced Raman spectroscopy (SERS) shows great promise to meet these criteria. In this novel assay, bacteria are captured and isolated using functionalized metal nanoparticles for rapid optical identification via SERS. Initial tests with a portable SERS system validated the ability to identify the presence of Escherichia coli and methicillin-resistant Staphylococcus aureus bacteria, quantifying concentrations down to 10 colony-forming units per mL (cfu/mL), with high reproducibility. The system was also able to discriminate between the bacteria within the same sample matrix at clinically relevant concentrations (103 cfu/mL), demonstrating its potential as a rapid, reliable on-site diagnostic for bacterial pathogens. [ABSTRACT FROM AUTHOR]
- Published
- 2019