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The article discusses the genetic anomalies that will help improve the diagnosis and prognosis of retinoblastoma. Topics mentioned include a brief description of retinoblastoma, the survival rates of patients with retinoblastoma, the importance of genetic testing, and the screening and treatment of retinoblastoma.

The article focuses on antibiotic heteroresistance, which refers to low-frequency resistant cells within bacterial isolates that are difficult to detect but may lead to treatment failure. Topics include the mechanisms behind heteroresistance, such as gene amplification, and its clinical implications, including the potential for treatment failure and challenges in detecting and addressing this form of resistance.

The article describes the viral corneal infections or herpes simplex virus (HSV) which remains the leading cause of herpetic ocular incidents. Diagnostic options for HSV include herpes culture-fluorescent antibody, DNA amplification, Tzanck smear, and serum antibody testing. Topical treatment strategies for HSV include antibiotics, steroids, and antivirals like trifluridine, vidarabine and ganciclovir.

The article offers information on two pathogen testing products for food safety. The 3M™ Molecular Detection System from 3M Food Safety combines bioluminescence detection and isothermal DNA amplification to accurately detect foodborne pathogens on the molecular level. The X-Mark™, a handheld nanoparticle-based biosensor from nanoRETE Inc., detects pathogens in an hour without culturing.

Features the Invader technology from amplifying DNA from Third Wave Technologies Inc. in Madison, Wisconsin. Mechanism of Invader technology; Use of the rolling circle amplification technique; Accumulation of fluorescent molecules.

The article offers information on research by X. Sunney Xie and colleagues on development of whole-genome amplification method LIANTI which reduces incomplete sequence coverage. Topics discussed include fragmenting genomic DNA from a single cell by inserting transposons; enabling detection of copy-number variation; and differences in initiation of DNA replication from cell to cell.

The article reports on the development of a gene amplification system in bacteria by researchers in Canada, Great Britain and Japan which can be used in the production of industrial antibiotics.

The article discusses key considerations for performing polymerase chain reaction for the amplification of DNA in the laboratory and for identifying pathogens. Significant considerations include the selection of oligonucleotide primer pair sequences, which are designed to identify complementary sources of specific microbes, choosing appropriate positive and negative controls and exercising caution in interpreting results.

Presents a question and answer advisory on using amplification treatment for infants and young children. Clarification on the need for different amplification characteristics by children with hearing impairments from adults with similar disorders; Explanation of the applicability of beneficial technological advances designed for adults on children; Identification of the outcome measures to determine the success of amplification process.

Cites the audiology keynote sessions presented during 2004 Audiology and Speech-Language Pathology Convention held November 18 to 20, 2004 in Philadelphia. Focus of the lectures on developments in hair cell regeneration, amplification and follow-up of hearing loss in infants; Offer of both practical and theoretic topics; Coverage on inner ear repair and regeneration by Sharon Kujawa; Discussion on the development of cochlear implants for treating sensorineural hearing loss by Douglas Cotanche; Exploration on central nervous system responses by Dianne Durham.

Reports on developments concerning gene amplification technologies. Caliper Life Sciences Corp.'s design of a chip that integrates reaction assembly, thermal cycling, and fluorescence detection; Cephied Inc.'s attempt to miniaturize bioanalytical testing by integrating a fluorimeter in which polymerase chain reaction (PCR) reactions can be conducted and which also contains a four-channel optical analysis system; Biotrove's creation of nanometer plates that replaces wells with through-holes, which are hydrophilic on the inside and hydrophobic on the rim.

The article focuses on a study according to which MALBAC, DNA amplication technique, will improve the single-cell genome sequencing method. It states that the method is suitable for multiple annealing and looping-based amplification cycles because the linear amplification step reduces the sequencing bias. It further states that the method will also help to detect copy number variations.

The article reports on the approval by the U.S. Food and Drug Administration (FDA) of the first nucleic acid amplification test to diagnose Q fever infections.

The article reports on the development of the non-polymerase chain reaction (PCR) exponential amplification method for aqueous systems by Doron Shabat and Eran Sella of Tel Aviv University in Tel Aviv, Israel. It mentions that the method is used to improve the sensitivity of the diagnostic tests. It states that the method was made through a modular and branched molecule named dendron which has the capacity to be disassembled when it reacts with a target analyte.

The article provides information on the amplification process of molecules that have the ability to catalyze themselves. In a highlighted incident when researchers Douglas Philp and Jan W. Sadownik were testing four reagents' that transform into imines and reactive nitrones, the nitrones were accordingly found to have undergone irreversible dipolar cycloaddion reactions. Meanwhile, an illustration of the chemical reaction is likewise presented in order to fully analyze the chemical reaction.

The article reports that geneticists from the Harvard University have improved an enzymatic amplification method used to generate copies of the DNA in single cells. The geneticists used a fluorescent dye to monitor the amplification in real time. They discovered that the amplification method generates hyperbranched DNA.

Reports on the discovery of the missing genes of Caenorhabditis elegans previously missed by genome analysis methods. Results of extending the original computer program to the genome of C. elegans; Total number of genes identified using the novel method; Propositions from researchers that amplifying and sequencing the predicted genes from a more accurate gene-prediction program would provide a more complete annotation.

Cites the importance of research to the field of communication science and disorders. Identification of the topics in vol.9, December 14, 2004 issue of “The ASHA Leader” journal showing the application of research to link theory with medical practice; Intervention decision making; Phonological disorders; Neurorehabilitation; Amplification for infants and children.

Presents an excerpt from the report "Gene Amplification Technologies: End Users, Marketers and Markets," by MarketResearch.com. Creation of technology that would allow miniaturized, self-contained, and fast DNA amplification reactions.