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For example, if quality control personnel get unexpectedly low plate-count results, the same sample can be analyzed by fl ow cytometry to determine whether cells are present or an input error occurred on the production line or the cells died in the product. Other advances in fl ow cytometry besides acoustic-assisted fl ow cytometry, she said, are spectral cytometry and mass cytometry. Pg 64 03.19 • www.ift.org by Neil H. Mermelstein [FOOD S AFETY & QUALITY] Applying Flow Cytometry to Food and Dietary Supplements F low cytometry has been used extensively as a clinical diagnostic tool, in pharmaceutical development, and in basic medical research since its development in the 1950s and 1960s. [Extracted from the article]

The article discusses the use of mass cytometry and inductively coupled plasma-mass spectroscopy (ICP-MS) in the field of single-cell biology. Topics discussed include the advantage of mass cytometry in isotope resolution, the components of mass cytometer technology such as time-of-flight (TOF), ICP, and ion optics, and the methods for determining the viable cells.

Presents a theoretical experiment comparing Western blot and flow cytometry with three samples and a protein of interest. Importance of discerning an identifiable protein signature; Indication on the advances in flow cytometry technology; Technique that measures the phosphorylation status of specific proteins.

Reports the technological innovations of flow cytometry in analyzing cells. Integration of fluorescence detection techniques, fluidics, electronics and computer technology; Application of the CyFlow Ultracompact Mobile Flow Cytometer; Cytometer systems for cell and organelle sorting. INSET: Some Applications of Flow Cytometry.

The article discusses a study which aimed to determine if apoptotic photoreceptor cell death in RPE65 gene knockout mice induced a retinal response that involved dendritic cells (DC). Topics covered include the potential role of DC in Leber congenital amaurosis (LCA), the results showing that DC migrate to regions of retinal stress to mediate neurodegeneration, and the implications of the presence and activity of DC for novel therapeutics for congenital retinal dystrophies.

The article offers information about a research conducted by electrical-engineering researchers on targeting white blood cells counting for treatment of Alzheimer's, Huntington's disease. Topics discussed include use of magnetic nanoparticle in targeting white blood cells; research by University of California, Los Angeles neuroscientists on astrocytes; and a research by Hoseon Lee of Kennesaw State University on flow cytometry.

The article presents a study which examines the potential of stem cell identification method in restoring vision to patients with damaged corneas. Researchers put pressure on particular cells to better understand their ability in transforming into mature cells using a technique which involves highly sensitive atomic force microscopy. Researchers also developed a microfluidic cell-sorting device which could speed up the process of cell sorting.

The article shows mechatronics trends meant for advancement of medical devices. Most medical devices are installed with micro actuators and micro sensors, resulting to development of devices for medical applications including flow cytometry, mobile miniature robots, and handheld diagnostics. The benefits of mechatronics like enhanced health monitoring and less office visits could cut costs by about 200 billion dollars in 25 years, as reported by the Center for Technology and Aging.

The article discusses completion of a project to reconfigure and revalidate Containment Level 3 laboratory suites at the Liverpool School of Tropical Medicine in Merseyside, England by healthcare solutions firm Medical Air Technology, which installed a Fluorescence Activated Cell Sorting machine.

The article reports on the use of impedance measurement and flow cytometry in performing blood analysis. The impedance measurement system provides accurate and fast analysis of constituent components of liquids and samples. Meanwhile, microscopic particles are suspended in a stream of fluid and individual cells are characterized in the flow cytometry system.

This article focuses on sample preparation for genomics. Sample preparation has historically been a research bottleneck because the available methods of purification were slow and labor-intensive. Now, international companies such as Agilent Technologies Inc., Amersham Biosciences, Applied Biosystems, Pall Corp., and Qiagen NV have been customizing solutions for individual labs. The new innovations in sample preparation are likely to come in the form of microfluidics and nanofluidics that will allow entire suites of experiments, from cell culture to cell sorting to cell lysing, to be incorporated into a single chip-based platform. INSET: Glimpsing the Future . . ..

Examines the effectiveness of the phase II trial of the iris-claw phakic IOL for the treatment of hyperopia. Results of hyperopia implants; Stability of refractions; Changes on endothelial cell counts.

The article focuses on the plans of Sysmex Corp., manufacturer of analytical equipment to expand its business in cell analysis and flow cytometry through the acquisition of two corporations in Japan. It mentions that with the acquisition of Inostics GmbH, diagnostics service company, Sysmex is planning to improve its portfolio in cancer diagnostics.

The article previews the Ekomilk Scan portable analyser from Southwest Scientific.

The article focuses on products and services provided by Beckman Coulter Life Sciences. It reports that the company majorly deals in developing products that can automate complex biomedical testing of several systems which include particle characterization, centrifugation and flow cytometry. It informs about several products developed by the company which include the CESl 8000 High Performance Separation-ESl Module with OptiMS technology, the PA 800 plus Pharmaceutical Analysis System.

The article offers information on Iridian Spectral Technologies Ltd. located in Ottawa, Ontario. It mentions that the company provides solutions for Raman spectroscopy, fluorescence and flow cytometry filters. It informs that the company offers improved product quality and customer services. It further highlights several aspects of the company including its major spectroscopic techniques, services and markets.

A chart is presented depicting the flow cytometry demand by various industries for 2011 such as academia, hospital-clinic, and independent testing-contract research organization (CRO).

The article reports that Affymetrix will acquire flow cytometry and immunoassay reagents manufacturer eBioscience.

The article focuses on the capability of mass cytometry in improving cell analysis, as revealed in the results of a study that was published in a 2011 issue of the journal "Analytical Chemistry." Fluorescently labeled antibodies are being used by scientists when conducting flow cytometry in order to identify various types of cells. Chemist Scott D. Tanner explained the difference between flow cytometry and mass cytometry.

The article presents a discussion about data analysis by flow cytometry, adapted from the article "Cell Analysis: Instruments, Equipment, Cell Culture, and More," by Strategic Directions Interntional Inc.

The article presents the results of a study on patients with multiple myeloma who underwent chemotherapy followed by autologous stem cell transplantation (ASCT). The study found that minimal residual disease (MRD) as assessed by multiparameter flow cytometry was found to be the most important prognostic factor for survival (PFS) among newly diagnosed multiple myeloma patients who underwent ASCT. Findings show that patients who were MRD-negative at 100 days had significantly longer PFS than patients who were MRD-positive.

The article evaluates the Hepatic Reductase Null (HRN) mouse from Taconic for studies in drug efficacy and lead optimization and the IOTest flow cytometry reagents from Beckman Coulter

The article offers information on the North American clinical flow cytometry market. It also discusses the benefits and the advantages of flow cytometry instruments. According to the author, clinical flow cytometry accounts a significant share of the overall flow cytometry market. About $73 million was accounted for the North American clinical flow cytometers market in 2006.

The article focuses on the business operations of Semrock Inc. It manufactures high-performance optical filters used in biotechnology. Semrock consistently delivers sophisticated filters. The company's chief spectroscopic techniques include flow cytometry and raman spectroscopy. Location of various facilities are also cited.

Sexed pig semen may be a step nearer to commercial application after a successful European experiment. The trial used a new artificial insemination catheter to inseminate three sows, one with female sexed semen and two with unsexed semen. One of the sows inseminated with unsexed semen produced six male and two female piglets at 113 days after insemination. While previous sexed semen trials have yielded successful results, they relied on surgical insemination methods. Semen for the trial was sexed by flow cytometry. Using a new insemination catheter, semen was delivered to the uterine horn, reducing the number of sperm required.

Reports on the cell-sorter developed by the California Institute of Technology. Ability to separate the microorganisms during flow cytometry; Use of a rubber chip and a reader; Computer as decision-maker in analyzing the electrical signal generated by the fluorescence generated by the cell.