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Research tools that can decipher protein-protein interactions and binding interface contact points can aid in the successful development of biotherapeutics. Hydroxyl radical footprinting-mass spectrometry (HRF-MS) technologies are being developed as tools for deciphering protein-protein interactions. We have demonstrated the utility of using fast photochemical oxidation of proteins (FPOP) as an HRF-MS technology for biotherapeutic protein structural characterization and analysis of protein-protein interfaces; monoclonal antibody (mAb) epitope mapping has also been demonstrated using this technique. However, the postlabeling workflow that utilizes offline protein digestion and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis is labor intensive and time consuming, and has significant sample consumption. In the present work, we demonstrate the potential of multidimensional online peptide mapping analysis (reduction, tryptic digestion, and separation) as a strategy for improving our postlabeling workflow for proteinprotein interactions. The proof-of-concept studies were performed using a Fab antibody fragment and its antigen binding partner. [ABSTRACT FROM AUTHOR]

The article discusses the improvement of pathogenic understanding of infantile and adult-onset cataracts by identifying genetic variants that cause them. Topics covered include physiological lens development, the independent occurrence of childhood cataracts from other ocular abnormalities, and congenital cataracts' link to genetic and phenotypic heterogeneity. Also noted is the timing of cataract appearance based on accumulated cataractogenic load.

At Carnegie Mellon University has developed a technique for 3D printing tissue scaffolds out of collagen, the major structural protein in the human body. "Collagen is an extremely desirable biomaterial to 3D print with because it makes up literally every single tissue in your body", says Andrew Hudson, a biomedical engineering Ph.D. student and researcher. The Fresh bioprinting method lets collagen be deposited layer-by-layer within supporting gel bath, letting the collagen solidify before it is removed from the support bath. [Extracted from the article]

The article focuses on the use of bone material from cattle to repair or replace bone tissue including need for the bone to shed its bovine biochemistry to avoid triggering an immune response in humans; and role of substances like cellulose gum to restore some of the lost cohesion structural proteins.

The author reflects on the continued search of engineers, scientists and doctors for answers to figure out how the actions of motor and cytoskeletal proteins such as actin and myosin lead to the contraction of muscle fibers, which can help lead to therapies and treatments for muscular disorders. He mentions the journal's cover story, "The Secret Life of the Spine" by William S. Marras, which focuses on the load and tolerance relationship.

Cites a study which found that the primary cellular target of bistramide A is actin. Functions of the actin; Citations of the aspect wherein the activity of bistramide A was attributed; Identification of the targets of bioactive compounds.