1. Affinity-Based Strategies for PROTEIN PURIFICATION.
- Author
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Mondal, Kalyani and Roy, Ipsita
- Subjects
- *
ANALYTICAL chemistry , *PROTEIN fractionation , *METHODOLOGY , *PRECIPITATION (Chemistry) , *SALTS , *ORGANIC solvents , *POLYMERS , *ACETONE , *ALCOHOL - Abstract
The article reports on the expanding tools for protein separation and the occurrence of two paradigm shifts in protein purification. This is through the use of affinity to develop nonchromatographic strategies and then applying either in purification or in a stand-alone approach. Techniques such as precipitation of salts, the use of water-miscible organic solvents such as acetone and ethyl alcohol and water soluble polymers such as poly(ethylene glycol), dextran and polyacrylates are the nonchromatographic approaches dominating the isolation of proteins.
- Published
- 2006
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