1. MitoTracker: A useful tool in need of better alternatives.
- Author
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Neikirk, Kit, Marshall, Andrea G., Kula, Bartosz, Smith, Nathan, LeBlanc, Sharonda, and Hinton, Antentor
- Subjects
- *
BASIC dyes , *MITOCHONDRIA , *ORGANELLES , *EXPERIMENTAL design , *FLUORESCENT probes - Abstract
The fluorescence viewing of mitochondria is commonly performed by MitoTracker, a lipophilic cationic dye that is taken up by the mitochondria. In this forum, we highlight several issues that may occur with MitoTracker, including staining of other organelles. Our aim is to offer alternative dyes and discuss their advantages and disadvantages. We also offer options for software with alternatives to MitoTracker to expedite future experimental design. Descriptions of all the types of MitoTracker and their relative abilities. Drawbacks of MitoTracker include affecting mitochondrial function. Common alternatives discussing their relative benefits and disadvantages for MitoTracker. Limitations of MitoTracker and presents alternative dyes for more specific uses. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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