1. Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals
- Author
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Awel, Salah, Kirian, Richard A., Wiedorn, Max O., Beyerlein, Kenneth R., Roth, Nils, Horke, Daniel A., Oberthür, Dominik, Knoska, Juraj, Mariani, Valerio, Morgan, Andrew, Adriano, Luigi, Tolstikova, Alexandra, Xavier, P. Lourdu, Yefanov, Oleksandr, Aquila, Andrew, Barty, Anton, Roy-Chowdhury, Shatabdi, Hunter, Mark S., James, Daniel, Robinson, Joseph S., Weierstall, Uwe, Rode, Andrei V., Bajt, Saša, Küpper, Jochen, and Chapman, Henry N.
- Subjects
Physics - Biological Physics - Abstract
We demonstrate near-atomic-resolution Bragg diffraction from aerosolized single granulovirus crystals using an x-ray free-electron laser. The form of the aerosol injector is nearly identical to conventional liquid-microjet nozzles, but the x-ray-scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. This approach provides a route to study the weak diffuse or lattice-transform signal arising from small crystals. The high speed of the particles is particularly well suited to upcoming MHz-repetition-rate x-ray free-electron lasers.
- Published
- 2017
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