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The Antiproton Decelerator (AD) at CERN provides antiproton bunches with a kinetic energy of 5.3 MeV. The Extra-Low ENergy Antiproton ring at CERN, commissioned at the AD in 2018, now supplies a bunch of electron-cooled antiprotons at a fixed energy of 100 keV. The MUSASHI antiproton trap was upgraded by replacing the radio-frequency quadrupole decelerator with a pulsed drift tube to re-accelerate antiprotons and optimize the injection energy into the degrader foils. By increasing the beam energy to 119 keV, a cooled antiproton accumulation efficiency of (26 +- 6)% was achieved., Comment: 22 pages, 8 figures

The ASACUSA-Cusp collaboration has recently upgraded the positron system to improve the production of antihydrogen. Previously, the experiment suffered from contamination of the vacuum in the antihydrogen production trap due to the transfer of positrons from the high pressure region of a buffer gas trap. This contamination reduced the lifetime of antiprotons. By adding a new positron accumulator and therefore decreasing the number of transfer cycles, the contamination of the vacuum has been reduced. Further to this, a new rare gas moderator and buffer gas trap, previously used at the Aarhus University, were installed. Measurements from Aarhus suggested that the number of positrons could be increased by a factor of four in comparison to the old system used at CERN. This would mean a reduction of the time needed for accumulating a sufficient number of positrons (of the order of a few million) for an antihydrogen production cycle. Initial tests have shown that the new system yields a comparable number of positrons to the old system., Comment: 10 pages, 5 figures, under consideration for the Special Collection "Non-Neutral Plasmas: Achievements and Perspectives" in JPP

The ASACUSA Cusp experiment requires the production of dense positron plasmas with a high repetition rate to produce a beam of antihydrogen. In this work, details of the positron production apparatus used for the first observation of the antihydrogen beam, and subsequent measurements are described in detail. This apparatus replaced the previous compact trap design resulting in an improvement in positron accumulation by a factor of ($52\pm3)$, Comment: 9 pages, 7 figures

Methods for reducing the radius, temperature, and space charge of nonneutral plasma are usually reported for conditions which approximate an ideal Penning Malmberg trap. Here we show that (1) similar methods are still effective under surprisingly adverse circumstances: we perform SDR and SDREVC in a strong magnetic mirror field using only 3 out of 4 rotating wall petals. In addition, we demonstrate (2) an alternative to SDREVC, using e-kick instead of EVC and (3) an upper limit for how much plasma can be cooled to T < 20 K using EVC. This limit depends on the space charge, not on the number of particles or the plasma density., Comment: Version 2: a small discrepancy between the N values for Table 1 and Fig. 3 led to an investigation of the charge counting diagnostic. There is a small energy dependence which only became apparent following improvements to pre-SDREVC. The pulsed dump was modified to reduce this dependence. The data for Table 1 and Fig. 3 was taken again with the improved methods

The goal of the ASACUSA (Atomic Spectroscopy And Collisions Using Slow Antiprotons) CUSP experiment at CERN's Antiproton Decelerator is to measure the ground state hyperfine splitting of antihydrogen in order to test whether CPT invariance is broken. The ASACUSA hodoscope is a detector consisting of two layers of 32 plastic scintillator bars individually read out by two serially connected silicon photo multipliers (SiPMs) on each end. Two additional layers for position resolution along the beam axis were scintillator fibres, which will now be replaced by scintillating tiles placed onto the existing bars and also read out by SiPMs. If the antiproton of antihydrogen annihilates in the center of the hodoscope, particles (mostly pions) are produced and travel through the various layers of the detector and produce signals. The hodoscope was successfully used during the last data taking period at CERN. The necessary time resolution to discriminate between particles travelling through the detector from outside and particles produced in the center of the detector was achieved by the use of waveform digitisers and software constant fraction discrimination. The disadvantage of this readout scheme was the slow readout speed, which was improved by two orders of magnitude. This was done by omitting the digitisers and replacing them with TDCs reading out the digital time-over-threshold (ToT) signal using leading edge discrimination., Comment: Submitted to VCI2022 Proceedings in NIM A

Magnetized nonneutral plasma composed of electrons or positrons couples to the local microwave environment via cyclotron radiation. The equilibrium plasma temperature depends on the microwave energy density near the cyclotron frequency. Fine copper meshes and cryogenic microwave absorbing material were used to lower the effective temperature of the radiation environment in ASACUSA's Cusp trap, resulting in significantly reduced plasma temperature.

The ASACUSA collaboration produces a beam of antihydrogen atoms by mixing pure positron and antiproton plasmas in a strong magnetic field with a double cusp geometry. The positrons cool via cyclotron radiation inside the cryogenic trap. Low positron temperature is essential for increasing the fraction of antihydrogen atoms which reach the ground state prior to exiting the trap. Many experimental groups observe that such plasmas reach equilibrium at a temperature well above the temperature of the surrounding electrodes. This problem is typically attributed to electronic noise and plasma expansion, which heat the plasma. The present work reports anomalous heating far beyond what can be attributed to those two sources. The heating seems to be a result of the axially open trap geometry, which couples the plasma to the external (300 K) environment via microwave radiation., Comment: Proceedings of the Exotic Atoms (EXA) Conference, Vienna, 2021

We have isolated a cDNA clone for spicule matrix protein, SM30, from sea urchin Hemicentrotus pulcherrimus and have studied the expression of this gene in comparison with that of another spicule matrix protein gene, SM50. In cultured micromeres as well as in intact embryos transcripts of SM30 were first detectable around the onset of spicule formation and rapidly increased with the growth of spicules, which accompanied accumulation of glycosylated SM30 protein(s). When micromeres were cultured in the presence of Zn2+, spicule formation and SM30 expression were suppressed, while both events resumed concurrently after the removal of Zn2+ from the culture medium. Expression of SM50, in contrast, started before the appearance of spicules and was not sensitive to Zn2+. Differences were also observed in adult tissues; SM30 mRNA was detected in spines and tube feet but not in the test, while SM50 mRNA was apparent in all of these mineralized tissues at similar levels. These results strongly suggest that the SM30 gene is regulated by a different mechanism to that of the SM50 gene and that the products of these two genes are differently involved in sea urchin biomineralization. A possible role of SM30 protein in skeleton formation is discussed.

In the present study, we enumerate and characterize the proteins that comprise the integral spicule matrix of the Strongylocentrotus purpuratus embryo. Two-dimensional gel electrophoresis of [35S]methionine radiolabeled spicule matrix proteins reveals that there are 12 strongly radiolabeled spicule matrix proteins and approximately three dozen less strongly radiolabeled spicule matrix proteins. The majority of the proteins have acidic isoelectric points; however, there are several spicule matrix proteins that have more alkaline isoelectric points. Western blotting analysis indicates that SM50 is the spicule matrix protein with the most alkaline isoelectric point. In addition, two distinct SM30 proteins are identified in embryonic spicules, and they have apparent molecular masses of approximately 43 and 46 kDa. Comparisons between embryonic spicule matrix proteins and adult spine integral matrix proteins suggest that the embryonic 43-kDa SM30 protein is an embryonic isoform of SM30. An adult 49-kDa spine matrix protein is also identified as a possible adult isoform of SM30. Analysis of the SM30 amino acid sequences indicates that a portion of SM30 proteins is very similar to the carbohydrate recognition domain of C-type lectin proteins.