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Accurate and robust recording and decoding from the central nervous system (CNS) is essential for advances in human-machine interfacing. However, technologies used to directly measure CNS activity are limited by their resolution, sensitivity to interferences, and invasiveness. Advances in muscle recordings and deep learning allow us to decode the spiking activity of spinal motor neurons (MNs) in real time and with high accuracy. MNs represent the motor output layer of the CNS, receiving and sampling signals originating in different regions in the nervous system, and generating the neural commands that control muscles. The input signals to MNs can be estimated from the MN outputs. Here we argue that peripheral neural interfaces using muscle sensors represent a promising, non-invasive approach to estimate some neural activity from the CNS that reaches the MNs but does not directly modulate force production. We also discuss the evidence supporting this concept, and the necessary advances to consolidate and test MN-based CNS interfaces in controlled and real-world settings.

The decomposition of high-density surface electromyography (HD-sEMG) signals into motor unit discharge patterns has become a powerful tool for investigating the neural control of movement, providing insights into motor neuron recruitment and discharge behavior. However, current algorithms, while very effective under certain conditions, face significant challenges in complex scenarios, as their accuracy and motor unit yield are highly dependent on anatomical differences among individuals. This can limit the number of decomposed motor units, particularly in challenging conditions. To address this issue, we recently introduced Swarm-Contrastive Decomposition (SCD), which dynamically adjusts the separation function based on the distribution of the data and prevents convergence to the same source. Initially applied to intramuscular EMG signals, SCD is here adapted for HD-sEMG signals. We demonstrated its ability to address key challenges faced by existing methods, particularly in identifying low-amplitude motor unit action potentials and effectively handling complex decomposition scenarios, like high-interference signals. We extensively validated SCD using simulated and experimental HD-sEMG recordings and compared it with current state-of-the-art decomposition methods under varying conditions, including different excitation levels, noise intensities, force profiles, sexes, and muscle groups. The proposed method consistently outperformed existing techniques in both the quantity of decoded motor units and the precision of their firing time identification. For instance, under certain experimental conditions, SCD detected more than three times as many motor units compared to previous methods, while also significantly improving accuracy. These advancements represent a major step forward in non-invasive EMG technology for studying motor unit activity in complex scenarios.

Decoding nervous system activity is a key challenge in neuroscience and neural interfacing. In this study, we propose a novel neural decoding system that enables unprecedented large-scale sampling of muscle activity. Using micro-electrode arrays with more than 100 channels embedded within the forearm muscles, we recorded high-density signals that captured multi-unit motor neuron activity. This extensive sampling was complemented by advanced methods for neural decomposition, analysis, and classification, allowing us to accurately detect and interpret the spiking activity of spinal motor neurons that innervate hand muscles. We evaluated this system in two healthy participants, each implanted with three electromyogram (EMG) micro-electrode arrays (comprising 40 electrodes each) in the forearm. These arrays recorded muscle activity during both single- and multi-digit isometric contractions. For the first time under controlled conditions, we demonstrate that multi-digit tasks elicit unique patterns of motor neuron recruitment specific to each task, rather than employing combinations of recruitment patterns from single-digit tasks. This observation led us to hypothesize that hand tasks could be classified with high precision based on the decoded neural activity. We achieved perfect classification accuracy (100%) across 12 distinct single- and multi-digit tasks, and consistently high accuracy (>96\%) across all conditions and subjects, for up to 16 task classes. These results significantly outperformed conventional EMG classification methods. The exceptional performance of this system paves the way for developing advanced neural interfaces based on invasive high-density EMG technology. This innovation could greatly enhance human-computer interaction and lead to substantial improvements in assistive technologies, offering new possibilities for restoring motor function in clinical applications.

Following limb amputation, neural signals for limb functions persist in the residual peripheral nerves. Targeted muscle reinnervation (TMR) allows to redirected these signals into spare muscles to recover the neural information through electromyography (EMG). However, a significant challenge arises in separating distinct neural commands redirected from the transferred nerves to the muscles. Disentangling overlapping signals from EMG recordings remains complex, as they can contain mixed neural information that complicates limb function interpretation. To address this challenge, Regenerative Peripheral Nerve Interfaces (RPNIs) surgically partition the nerve into individual fascicles that reinnervate specific muscle grafts, isolating distinct neural sources for more precise control and interpretation of EMG signals. We introduce a novel biointerface that combines TMR surgery of polyvalent nerves with a high-density micro-electrode array implanted at a single site within a reinnervated muscle. Instead of surgically identifying distinct nerve fascicles, our approach separates all neural signals that are re-directed into a single muscle, using the high spatio-temporal selectivity of the micro-electrode array and mathematical source separation methods. We recorded EMG signals from four reinnervated muscles while volunteers performed phantom limb tasks. The decomposition of these signals into motor unit activity revealed distinct clusters of motor neurons associated with diverse functional tasks. Notably, our method enabled the extraction of multiple neural commands within a single reinnervated muscle, eliminating the need for surgical nerve division. This approach not only has the potential of enhancing prosthesis control but also uncovers mechanisms of motor neuron synergies following TMR, providing valuable insights into how the central nervous system encodes movement after reinnervation.

The cortico-spinal neural pathway is fundamental for motor control and movement execution, and in humans it is typically studied using concurrent electroencephalography (EEG) and electromyography (EMG) recordings. However, current approaches for capturing high-level and contextual connectivity between these recordings have important limitations. Here, we present a novel application of statistical dependence estimators based on orthonormal decomposition of density ratios to model the relationship between cortical and muscle oscillations. Our method extends from traditional scalar-valued measures by learning eigenvalues, eigenfunctions, and projection spaces of density ratios from realizations of the signal, addressing the interpretability, scalability, and local temporal dependence of cortico-muscular connectivity. We experimentally demonstrate that eigenfunctions learned from cortico-muscular connectivity can accurately classify movements and subjects. Moreover, they reveal channel and temporal dependencies that confirm the activation of specific EEG channels during movement.

Biosignal acquisition is key for healthcare applications and wearable devices, with machine learning offering promising methods for processing signals like surface electromyography (sEMG) and electroencephalography (EEG). Despite high within-session performance, intersession performance is hindered by electrode shift, a known issue across modalities. Existing solutions often require large and expensive datasets and/or lack robustness and interpretability. Thus, we propose the Spatial Adaptation Layer (SAL), which can be prepended to any biosignal array model and learns a parametrized affine transformation at the input between two recording sessions. We also introduce learnable baseline normalization (LBN) to reduce baseline fluctuations. Tested on two HD-sEMG gesture recognition datasets, SAL and LBN outperform standard fine-tuning on regular arrays, achieving competitive performance even with a logistic regressor, with orders of magnitude less, physically interpretable parameters. Our ablation study shows that forearm circumferential translations account for the majority of performance improvements, in line with sEMG physiological expectations., Comment: ICASSP(submitted), 5 pages

A major outstanding problem when interfacing with spinal motor neurons is how to accurately compensate for non-stationary effects in the signal during source separation routines, particularly when they cannot be estimated in advance. This forces current systems to instead use undifferentiated bulk signal, which limits the potential degrees of freedom for control. In this study we propose a potential solution, using an unsupervised learning algorithm to blindly correct for the effects of latent processes which drive the signal non-stationarities. We implement this methodology within the theoretical framework of a quasilinear version of independent component analysis (ICA). The proposed design, HarmonICA, sidesteps the identifiability problems of nonlinear ICA, allowing for equivalent predictability to linear ICA whilst retaining the ability to learn complex nonlinear relationships between non-stationary latents and their effects on the signal. We test HarmonICA on both invasive and non-invasive recordings both simulated and real, demonstrating an ability to blindly compensate for the non-stationary effects specific to each, and thus to significantly enhance the quality of a source separation routine.

Glioma cells can reshape functional neuronal networks by hijacking neuronal synapses, leading to partial or complete neurological dysfunction. These mechanisms have been previously explored for language functions. However, the impact of glioma on sensorimotor functions is still unknown. Therefore, we recruited a control group of patients with unaffected motor cortex and a group of patients with glioma-infiltrated motor cortex, and recorded high-density electrocortical signals during finger movement tasks. The results showed that glioma suppresses task-related synchronization in the high-gamma band and reduces the power across all frequency bands. The resulting atypical motor information transmission model with discrete signaling pathways and delayed responses disrupts the stability of neuronal encoding patterns for finger movement kinematics across various temporal-spatial scales. These findings demonstrate that gliomas functionally invade neural circuits within the motor cortex. This result advances our understanding of motor function processing in chronic disease states, which is important to advance the surgical strategies and neurorehabilitation approaches for patients with malignant gliomas.

Space poses significant challenges for human physiology, leading to physiological adaptations in response to an environment vastly different from Earth. While these adaptations can be beneficial, they may not fully counteract the adverse impact of space-related stressors. A comprehensive understanding of these physiological adaptations is needed to devise effective countermeasures to support human life in space. This review focuses on the impact of the environment in space on the musculoskeletal system. It highlights the complex interplay between bone and muscle adaptation, the underlying physiological mechanisms, and their implications on astronaut health. Furthermore, the review delves into the deployed and current advances in countermeasures and proposes, as a perspective for future developments, wearable sensing and robotic technologies, such as exoskeletons, as a fitting alternative., Comment: 23 pages (including references), 8 figures and 318 references

The adoption of high-density electrode systems for human-machine interfaces in real-life applications has been impeded by practical and technical challenges, including noise interference, motion artifacts and the lack of compact electrode interfaces. To overcome some of these challenges, we introduce a wearable and stretchable electromyography (EMG) array, and present its design, fabrication methodology, characterisation, and comprehensive evaluation. Our proposed solution comprises dry-electrodes on flexible printed circuit board (PCB) substrates, eliminating the need for time-consuming skin preparation. The proposed fabrication method allows the manufacturing of stretchable sleeves, with consistent and standardised coverage across subjects. We thoroughly tested our developed prototype, evaluating its potential for application in both research and real-world environments. The results of our study showed that the developed stretchable array matches or outperforms traditional EMG grids and holds promise in furthering the real-world translation of high-density EMG for human-machine interfaces., Comment: This is the author's version of the manuscript published in MDPI Sensors journal - https://www.mdpi.com/1424-8220/24/6/1810 , This manuscript is in IEEE format - 8 pages, 5 figures, 1 table

Spinal cord injuries (SCIs) generally result in sensory and mobility impairments, with torso instability being particularly debilitating. Existing torso stabilisers are often rigid and restrictive. This paper presents an early investigation into a non-restrictive 1 degree-of-freedom (DoF) mechanical torso stabiliser inspired by devices such as centrifugal clutches and seat-belt mechanisms. Firstly, the paper presents a motion-capture (MoCap) and OpenSim-based kinematic analysis of the cable-based system to understand requisite device characteristics. The simulated evaluation resulted in the cable-based device to require 55-60cm of unrestricted travel, and to lock at a threshold cable velocity of 80-100cm/sec. Next, the developed 1-DoF device is introduced. The proposed mechanical device is transparent during activities of daily living, and transitions to compliant blocking when incipient fall is detected. Prototype behaviour was then validated using a MoCap-based kinematic analysis to verify non-restrictive movement, reliable transition to blocking, and compliance of the blocking., Comment: 4 pages, 4 figures, 10 references. Submitted to IEEE EMBC 2024 conference

sEMG pattern recognition algorithms have been explored extensively in decoding movement intent, yet are known to be vulnerable to changing recording conditions, exhibiting significant drops in performance across subjects, and even across sessions. Multi-channel surface EMG, also referred to as high-density sEMG (HD-sEMG) systems, have been used to improve performance with the information collected through the use of additional electrodes. However, a lack of robustness is ever present due to limited datasets and the difficulties in addressing sources of variability, such as electrode placement. In this study, we propose training on a collection of input channel subsets and augmenting our training distribution with data from different electrode locations, simultaneously targeting electrode shift and reducing input dimensionality. Our method increases robustness against electrode shift and results in significantly higher intersession performance across subjects and classification algorithms., Comment: 5 pages

Brain-computer interfaces (BCIs) provide a direct pathway from the brain to external devices and have demonstrated great potential for assistive and rehabilitation technologies. Endogenous BCIs based on electroencephalogram (EEG) signals, such as motor imagery (MI) BCIs, can provide some level of control. However, mastering spontaneous BCI control requires the users to generate discriminative and stable brain signal patterns by imagery, which is challenging and is usually achieved over a very long training time (weeks/months). Here, we propose a human-machine joint learning framework to boost the learning process in endogenous BCIs, by guiding the user to generate brain signals towards an optimal distribution estimated by the decoder, given the historical brain signals of the user. To this end, we firstly model the human-machine joint learning process in a uniform formulation. Then a human-machine joint learning framework is proposed: 1) for the human side, we model the learning process in a sequential trial-and-error scenario and propose a novel ``copy/new'' feedback paradigm to help shape the signal generation of the subject toward the optimal distribution; 2) for the machine side, we propose a novel adaptive learning algorithm to learn an optimal signal distribution along with the subject's learning process. Specifically, the decoder reweighs the brain signals generated by the subject to focus more on ``good'' samples to cope with the learning process of the subject. Online and psuedo-online BCI experiments with 18 healthy subjects demonstrated the advantages of the proposed joint learning process over co-adaptive approaches in both learning efficiency and effectiveness.

Numerical models of electromyographic (EMG) signals have provided a huge contribution to our fundamental understanding of human neurophysiology and remain a central pillar of motor neuroscience and the development of human-machine interfaces. However, whilst modern biophysical simulations based on finite element methods are highly accurate, they are extremely computationally expensive and thus are generally limited to modelling static systems such as isometrically contracting limbs. As a solution to this problem, we propose a transfer learning approach, in which a conditional generative model is trained to mimic the output of an advanced numerical model. To this end, we present BioMime, a conditional generative neural network trained adversarially to generate motor unit activation potential waveforms under a wide variety of volume conductor parameters. We demonstrate the ability of such a model to predictively interpolate between a much smaller number of numerical model's outputs with a high accuracy. Consequently, the computational load is dramatically reduced, which allows the rapid simulation of EMG signals during truly dynamic and naturalistic movements.

Adapting upper-limb impedance (i.e., stiffness, damping, inertia) is essential for humans interacting with dynamic environments for executing grasping or manipulation tasks. On the other hand, control methods designed for state-of-the-art upper-limb prostheses infer motor intent from surface electromyography (sEMG) signals in terms of joint kinematics, but they fail to infer and use the underlying impedance properties of the limb. We present a framework that allows a human user to simultaneously control the kinematics, stiffness, and damping of a simulated robot through wrist's flexion-extension. The framework includes muscle-tendon units and a forward dynamics block to estimate the motor intent from sEMG signals, and a variable impedance controller that implements the estimated intent on the robot, allowing the user to adapt the robot's kinematics and dynamics online. We evaluate our framework with 8 able-bodied subjects and an amputee during reaching tasks performed in free space, and in the presence of unexpected external perturbations that require adaptation of the wrist impedance to ensure stable interaction with the environment. We experimentally demonstrate that our approach outperforms a data-driven baseline in terms of its ability to adapt to external perturbations, overall controllability, and feedback from participants.

Understanding how movement is controlled by the central nervous system remains a major challenge, with ongoing debate about basic features underlying this control. In this review, we introduce a new conceptual framework for the distribution of common input to spinal motor neurons. Specifically, this framework is based on the following assumptions: 1) motor neurons are grouped into functional groups (clusters) based on the common inputs they receive; 2) clusters may significantly differ from the classical definition of motor neuron pools, such that they may span across muscles and/or involve only a portion of a muscle; 3) clusters represent functional modules used by the central nervous system to reduce the dimensionality of the control; and 4) selective volitional control of single motor neurons within a cluster receiving common inputs cannot be achieved. We discuss this framework and its underlying theoretical and experimental evidence., Comment: 12 pages; 1 figure; review paper or opinion piece

Advances in biosignal signal processing and machine learning, in particular Deep Neural Networks (DNNs), have paved the way for the development of innovative Human-Machine Interfaces for decoding the human intent and controlling artificial limbs. DNN models have shown promising results with respect to other algorithms for decoding muscle electrical activity, especially for recognition of hand gestures. Such data-driven models, however, have been challenged by their need for a large number of trainable parameters and their structural complexity. Here we propose the novel Temporal Convolutions-based Hand Gesture Recognition architecture (TC-HGR) to reduce this computational burden. With this approach, we classified 17 hand gestures via surface Electromyogram (sEMG) signals by the adoption of attention mechanisms and temporal convolutions. The proposed method led to 81.65% and 80.72% classification accuracy for window sizes of 300ms and 200ms, respectively. The number of parameters to train the proposed TC-HGR architecture is 11.9 times less than that of its state-of-the-art counterpart.

Neurophysiological time series, such as electromyographic signal and intracortical recordings, are typically composed of many individual spiking sources, the recovery of which can give fundamental insights into the biological system of interest or provide neural information for man-machine interfaces. For this reason, source separation algorithms have become an increasingly important tool in neuroscience and neuroengineering. However, in noisy or highly multivariate recordings these decomposition techniques often make a large number of errors, which degrades human-machine interfacing applications and often requires costly post-hoc manual cleaning of the output label set of spike timestamps. To address both the need for automated post-hoc cleaning and robust separation filters we propose a methodology based on deep metric learning, using a novel loss function which maintains intra-class variance, creating a rich embedding space suitable for both label cleaning and the discovery of new activations. We then validate this method with an artificially corrupted label set based on source-separated high-density surface electromyography recordings, recovering the original timestamps even in extreme degrees of feature and class-dependent label noise. This approach enables a neural network to learn to accurately decode neurophysiological time series using any imperfect method of labelling the signal., Comment: 16 pages, 7 figures

There has been a surge of recent interest in Machine Learning (ML), particularly Deep Neural Network (DNN)-based models, to decode muscle activities from surface Electromyography (sEMG) signals for myoelectric control of neurorobotic systems. DNN-based models, however, require large training sets and, typically, have high structural complexity, i.e., they depend on a large number of trainable parameters. To address these issues, we developed a framework based on the Transformer architecture for processing sEMG signals. We propose a novel Vision Transformer (ViT)-based neural network architecture (referred to as the TEMGNet) to classify and recognize upperlimb hand gestures from sEMG to be used for myocontrol of prostheses. The proposed TEMGNet architecture is trained with a small dataset without the need for pre-training or fine-tuning. To evaluate the efficacy, following the-recent literature, the second subset (exercise B) of the NinaPro DB2 dataset was utilized, where the proposed TEMGNet framework achieved a recognition accuracy of 82.93% and 82.05% for window sizes of 300ms and 200ms, respectively, outperforming its state-of-the-art counterparts. Moreover, the proposed TEMGNet framework is superior in terms of structural capacity while having seven times fewer trainable parameters. These characteristics and the high performance make DNN-based models promising approaches for myoelectric control of neurorobots.

Augmenting the body with artificial limbs controlled concurrently to the natural limbs has long appeared in science fiction, but recent technological and neuroscientific advances have begun to make this vision possible. By allowing individuals to achieve otherwise impossible actions, this movement augmentation could revolutionize medical and industrial applications and profoundly change the way humans interact with their environment. Here, we construct a movement augmentation taxonomy through what is augmented and how it is achieved. With this framework, we analyze augmentation that extends the number of degrees-of-freedom, discuss critical features of effective augmentation such as physiological control signals, sensory feedback and learning, and propose a vision for the field.

MagnetoMyoGraphy (MMG) is a method of studying muscle function via weak magnetic fields generated from human active organs and tissues. The correspondence between MMG and electromyography means directly derived from the Maxwell-Amp\`ere law. Here, upon briefly describing the principles of voltage distribution inside skeletal muscles due to the electrical stimulation, we provide a protocol to determine the effects of the magnetic field generated from a time-changing action potential propagating in a group of skeletal muscle cells. The position-dependent and the magnetic field behaviour on account of the different currents in muscle fibres are performed in temporal, spectral and spatial domains. The procedure covers identification of the fibre subpopulations inside the fascicles of a given nerve section, characterization of soleus skeletal muscle currents, check of axial intracellular currents, calculation of the generated magnetic field ultimately. We expect this protocol to take approximately 2-3 hours to complete for the whole finite-element analysis., Comment: To be submitted to Nature Protocols. 37 Pages and includes 10 figures

This work is motivated by the recent advances in Deep Neural Networks (DNNs) and their widespread applications in human-machine interfaces. DNNs have been recently used for detecting the intended hand gesture through processing of surface electromyogram (sEMG) signals. The ultimate goal of these approaches is to realize high-performance controllers for prosthetic. However, although DNNs have shown superior accuracy than conventional methods when large amounts of data are available for training, their performance substantially decreases when data are limited. Collecting large datasets for training may be feasible in research laboratories, but it is not a practical approach for real-life applications. Therefore, there is an unmet need for the design of a modern gesture detection technique that relies on minimal training data while providing high accuracy. Here we propose an innovative and novel "Few-Shot Learning" framework based on the formulation of meta-learning, referred to as the FS-HGR, to address this need. Few-shot learning is a variant of domain adaptation with the goal of inferring the required output based on just one or a few training examples. More specifically, the proposed FS-HGR quickly generalizes after seeing very few examples from each class. The proposed approach led to 85.94% classification accuracy on new repetitions with few-shot observation (5-way 5-shot), 81.29% accuracy on new subjects with few-shot observation (5-way 5-shot), and 73.36% accuracy on new gestures with few-shot observation (5-way 5-shot).

The performance of upper-limb prostheses is currently limited by the relatively poor functionality of unintuitive control schemes. This paper proposes to extract, from multichannel electromyographic signals (EMG), motor neuron spike trains and project them into lower dimensional continuous signals, which are used as multichannel proportional inputs to control the prosthetic's actuators. These control signals are an estimation of the common synaptic input that the motor neurons receive. We use the simplest of metric learning approaches known as principal component analysis (PCA), as a linear unsupervised metric projection to extract the spectral information of high dimensional data into the subspace of the prosthetic hand degrees of freedom. We also investigate the importance of a rotation in the projection space that best aligns the PCA subspace with the space of degrees of freedom of the prosthetic hand, to attempt to approximate the sparseness properties of the motor axes (which are orthogonal), while no sparseness is enforced by PCA. Proof of concept for the feedforward path (open loop) is given by successful estimation of concurrent movements with up to three degrees of freedom. We also analyze and quantify the performance of the proposed decoding algorithm under implementation constraints and hardware limitations and propose a space-time subsampling strategy, to maximize projection fidelity, in each extracted source over time. The results confirm that the proposed decoding approach can directly project the motor neuron spike trains into kinematics for simultaneous and proportional prosthesis control that can be extended to multiple degrees of freedom. We show that the method is robust to reducing the training data in space (number of sources) and time, which makes it potentially suitable for clinical applications., Comment: 24 pages, 11 figures, 3 tables