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Increasing use of hair to survey carnivore distribution and identify carnivore scavengers or predators at prey kill sites requires methods for cost‐efficient identification of predator hair. Although DNA analysis can be used to identify species‐specific hairs, reliability depends on quality of hair, collection method, and environmental conditions, with cost that can exceed CAD$35/hair. In contrast, features of guard hairs including hair length, banding, and macro‐ and microscopic characteristics of the hair cuticle and medulla offer an alternative approach when hair quality is poor or funding is limited. Past keys focused on hair identification of prey species (e.g., ungulates, rodents) in predator scat analysis or were general because they contained all mammals in a region, thus complicating the focus on dichotomous keys for large carnivores. We used Random Forest (RF) to identify features that best classified known‐origin guard hairs (n = 175) and used these characteristics to develop a dichotomous key for hair identification of the 7 major, large carnivore species common to the Rocky Mountains of Alberta, Canada. We found relative medulla width and pattern, cuticle‐scale characteristics, and hair length provided the greatest probability of correctly distinguishing among hairs of different carnivore species. Correct classification of within sample hairs with RF based on Area Under the Curve (AUC) averaged 0.95 ± 0.10, with coyote (Canis latrans) hairs having the lowest classification accuracy. Blind trials classifying 21 hairs using the dichotomous key yielded correct classifications of 88% ± 7% to the family level and 60% ± 10% to the species level. Hair preparation and identification by a trained technician was estimated at 30 ± 15 min/hair and CAD$8/hair. Our carnivore hair key provides an alternative approach to DNA hair analysis when either funds are limited, or hair samples are not of sufficient quality to be successfully sequenced. © 2018 The Wildlife Society. Past dichotomous hair keys focus on identification of prey species or contain all mammals in a region, with a lack of carnivore‐specific hair keys. We developed a dichotomous key specifically for identifying guard hairs of the 7 major, large carnivore species common to the Rocky Mountains of Canada. [ABSTRACT FROM AUTHOR]