1. [(Methyl)1-(11)c]-acetate metabolism in hepatocellular carcinoma.
- Author
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Salem, Nicolas, Yu Kuang, Corn, David, Erokwu, Bernadette, Kolthammer, Jeffrey A., Tian, Haibin, Chunying Wu, Fangjing Wang, Yanming Wang, Zhenghong Lee, Kuang, Yu, Wu, Chunying, Wang, Fangjing, Wang, Yanming, and Lee, Zhenghong
- Subjects
ACETIC acid ,ANIMAL experimentation ,CELL lines ,EPITHELIAL cells ,LIVER tumors ,RADIOISOTOPES ,RATS ,RESEARCH funding ,POSITRON emission tomography - Abstract
Purpose: Studies have established the value of [(methyl)1-(11)C]-acetate ([(11)C]Act) combined with 2-deoxy-2[(18)F]fluoro-D-glucose (FDG) for detecting hepatocellular carcinoma (HCC) using positron emission tomography (PET). In this study, the metabolic fate of [(11)C]Act in HCC was characterized.Methods: Experiments with acetic acid [1-(14)C] sodium salt ([(14)C]Act) were carried out on WCH-17 cells and freshly derived rat hepatocytes. PET scans with [(11)C]Act were also carried out on woodchucks with HCC before injection of [(14)C]Act. The radioactivity levels in different metabolites were quantified with thin-layer chromatography.Results: In WCH-17 cells, the predominant metabolite was phosphatidylcholine (PC). Regions of HCCs with the highest [(11)C]Act uptake had higher radioactivity accumulation in lipid-soluble compounds than surrounding hepatic tissues. In those regions, PC and triacylglycerol (TG) accumulated more radioactivity than in surrounding hepatic tissues.Conclusions: High [(11)C]Act uptake in HCC is associated with increased de novo lipogenesis. PC and TG are the main metabolites into which the radioactive label from [(11)C]Act is incorporated in HCC. [ABSTRACT FROM AUTHOR]- Published
- 2011
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