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1. Gene enrichment in maize with hypomethylated partial restriction (HMPR) libraries

Author

Emberton, John, Yinan Yuan, Jianxin Ma, SanMiguel, Philip, and Bennetzen, Jeffrey L.

Subjects
Nucleotide sequence -- Research, Methylation -- Research, Genetic research, Health
Abstract

A new technology was developed to assist gene-enrichment sequencing of any complex plant genome, employing maize as the test organism. Hypomethylated partial restriction (HMPR) libraries were constructed by using independent partial digestions with methylation-sensitive restriction enzymes Hpall and HpyCH41V.

Published
2005

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