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Fully grown oocytes have the natural ability to transform 2 terminally differentiated gametes into a totipotent zygote representing the acquisition of totipotency. This process wholly depends on maternal-effect factors (MFs). MFs stored in the eggs are therefore likely to be able to induce cellular reprogramming to a totipotency state. Here we report the generation of totipotent-like stem cells from mESCs using 4MFs Hsf1, Zar1, Padi6, and Npm2, designated as MFiTLSCs. MFiTLSCs exhibited a unique and inherent capability to differentiate into embryonic and extraembryonic derivatives. Transcriptomic analysis revealed that MFiTLSCs are enriched with 2-cell-specific genes that appear to synergistically induce a transcriptional repressive state, in that parental genomes are remodeled to a poised transcriptional repression state while totipotency is established following fertilization. This method to derive MFiTLSCs could help advance the understanding of fate determinations of totipotent stem cells in a physiological context and establish a foundation for the development of oocyte biology-based reprogramming technology.Graphical Abstract

Stent implantation is the mainstream treatment for high-incidence vascular diseases. The stent is implanted into the blocked vessel with minimal trauma to restore blood flow. Polymer braided stents with superior biocompatibility and flexibility have broad application prospects in stent implantation. An ideal polymer stent should have suitable radial supporting capacity to withstand the cyclic radial load from vessels. Especially in the case of accelerated vasoconstriction caused by emotional excitement, drinking or fever, etc. However, there are currently limited studies on the mechanical properties of stent at rapid radial loading. In this work, the radial supporting capacity and fatigue properties of polymer stent at rapid loading rate were investigated by experiment and simulation. With the increase of radial loading rate, the stent has different deformation tendency and fatigue resistance. This study is helpful to study the structural changes of the polymer braided stent at different loading rates, and provide ideas for the evaluation and the optimization of the polymer braided stent.

Physalis angulataLinn. is an exotic Amazonian fruit that is commonly recognized as wild tomato, winter cherry, and gooseberry. While its fruit is known to contain many nutrients, such as minerals, fibers, and vitamins, few papers have investigated withanolide derivatives from its fruits. UPLC-Q-Orbitrap-MS/MS, which produces fragmentation spectra, was applied for the first time to guide the isolation of bioactive withanolide derivatives from P. angulatafruits. As a result, twenty-six withanolide derivatives, including two novel 1,10-secowithanolides (1and 2) and a new derivative (3), were obtained. Compounds 1and 2are rare rearranged 1,10-secowithanolides with a tetracyclic 7/6/6/5 ring system. All structures were assigned through various spectroscopic data and quantum chemical calculations. Nine withanolide derivatives exhibited significant inhibitory effects on three tumor cell lines with IC50values of 0.51–13.79 μM. Moreover, three new compounds (1–3) exhibited potential nitric oxide inhibitory effects in lipopolysaccharide-stimulated RAW264.7 cells (IC50: 7.51–61.8 μM). This investigation indicated that fruits of P. angulatacould be applied to treat and prevent cancer and inflammatory-related diseases due to their potent active withanolide derivatives.

The purpose of this trial was to assess the safety and effectiveness of a paclitaxel-coated balloon catheter in Chinese patients with de novoor nonstented restenotic femoropopliteal atherosclerotic lesions.

Advanced safe battery storage systems with health prognostic performance are vital for electric vehicles. Various faults of lithium-ion batteries are usually undetectable in their early stage due to their concealment and graduality. This article presents a real-time fault diagnosis and isolation scheme for real-scenario batteries using the normalized discrete wavelet decomposition. The early frequency-domain features of the fault signals are extracted utilizing the high-frequency detail wavelet components, and a multilevel fault prognosis strategy is developed considering complex charging/driving characteristics under real-vehicle operating conditions. The verification results, implemented on loose wire connection batteries and real-scenario thermal runaway batteries, demonstrate that the proposed method can accurately extract and locate the hidden fault signals even under small magnitudes and effectively detecting and isolating battery faults before thermal runaway. Furthermore, significant reliability and stability of the proposed method are verified on more real-vehicle operation data, enabling online monitorable and traceable of battery faults before triggering thermal runaway, safeguarding drivers and passengers in real-world vehicular operation.

Hypersampones A–C (1–3), three unprecedented nor-polycyclic polyprenylated acylphloroglucinols (PPAPs), were isolated from Hypericum sampsonii. These compounds represent the first nor-PPAPs with an unexpected tetracyclic 6/5/5/6 ring system. Their structures were assigned through the analysis of detailed spectroscopic data, X-ray crystallography, and electronic circular dichroism calculations. Compound 1significantly inhibited the accumulation of lipid in an oleic acid-treated HepG2 cell model by suppressing the protein expression of FAS and ACACA at 5 μM.

At present, mining haul trucks (MHTs) directly deploy the on‐road heavy‐duty trucks’ battery‐electric powertrain, as they can cut down costs and emissions in mining. However, the operating patterns of MHT are different, e.g., ultraduty, low‐speed, and continuous road slopes, resulting in a mismatch between the dynamic and economic performance of mining required and the MHT achieved. The powertrain design and control influence the dynamic and economic performance, which can be quantitatively measured by top speed, gradeability, and energy consumption. This study uses an improved differential evolutionary algorithm to develop an integrated optimization platform to obtain the components sizing, gear ratio, and shifting schedule for the dedicated battery‐electric MHT. Mathematical models are established and validated using on‐site experiment data. An integrated optimization platform is initiated by concurrently formulating the motor sizing, gear ratio, and shifting schedule and solved by the improved differential evolutionary algorithm. Optimization results indicate that the economic performance is enhanced by 10.82%, 11.08%, 11.18%, and 11.20%, respectively, while maintaining or slightly improving the dynamic performance. Besides, the achievable maximum speed at the most common grade is boosted by 11.82%, 6.52%, 7.44%, and 6.52%, respectively. The study provides an approach to developing a battery‐electric powertrain for MHT. An integrated optimization platform is introduced to jointly optimize the design and control. The established mathematical models are validated using onsite experimental data. A novel evolutionary strategy based on design space segmentation is proposed. Motor size, gear ratios, and shift schedule are formulated and solved simultaneously. The dynamic and economic performance of mining haul truck is improved.

We report a Cu2O/CdS/ZnO photoanode constructed by coupling Cu2O and CdS nanoparticles on ZnO nanotube arrays (NTAs) via combining chemical bath deposition with successive ionic-layer adsorption and reaction. Integrating the merits of the superior ability of Cu2O and CdS to harvest visible light, dual heterojunctions, type-II band structure, a p-n junction, and ordered tubular structure, the photoanode exhibits simultaneous significant improvements in the visible light absorption, charge separation, hydrogen generation, and stability. At 0.4 V versus Ag/AgCl under AM 1.5 G irradiation, the photoanode achieves a photocurrent density of 7 mA/cm2, which is 2.3-fold higher than that of CdS/ZnO heterojunction NTAs. Furthermore, under AM 1.5 G illumination without bias potential, the photoanode achieves an average hydrogen generation rate of 134 μmol/h, which is 1.5 times that of single-heterojunction CdS/ZnO NTAs. The highest solar-to-hydrogen conversion efficiency of the Cu2O/CdS/ZnO photoanode is 2.8% at 0.6 V vs. RHE, 1.6 times that of the CdS/ZnO NTAs.

Purpose: The purpose was to evaluate the clinical outcomes of a dedicated venous stent with the tripartite composite segments for the treatment of iliofemoral venous obstruction (IVO) in a mixed cohort of nonthrombotic iliac vein lesion (NIVL) and post-thrombotic syndrome (PTS) over a period of 12 months.Methods: The Grency Trial is a prospective, multicenter, single-arm, open-label, pivotal study, which was conducted at 18 large tertiary hospitals in China from August 2019 to October 2020. A total of 133 hospitalized patients were screened and 110 patients with clinical, etiology, anatomical, and pathophysiology clinical class (CEAP) clinical grade C>3 and iliac vein stenosis >50% or occlusion, including 72 patients with NIVL and 38 patients with PTS, were implanted with Grency venous stents. Primary endpoint was stent patency at 12 months follow-up, and secondary outcomes were technical success; improvement in venous clinical severity score (VCSS) at 3, 6, and 12 month follow-up; and rates of clinical adverse events.Results: Among 110 patients who were implanted with Grency venous stents, 107 patients completed the 12 month follow-up. All 129 stents were successfully implanted in 110 limbs. Twelve-month primary patency rate was 94.39% [95% confidence interval [CI]=88.19%-97.91%] overall, and 100% [94.94%-100%] and 83.33% [67.19%-93.63%] in the NIVL and PTS subgroups, respectively. Venous clinical severity score after iliac vein stenting improved significantly up to 12 months follow-up. There were 3 early major adverse events (1 intracerebral hemorrhage and 2 stent thrombosis events related to anticoagulation therapy), and 7 late major adverse events (1 cardiovascular death, 1 intracranial hemorrhage with uncontrolled hypertension, and 5 in-stent restenosis cases without stent fractures or migration).Conclusions: The Grency venous stent system appeared excellent preliminary safe and effective for IVO treatment. Further large-scale studies with longer-term follow-up are needed to evaluate long-term patency and durability of stent.Clinical impact The design of venous stents for iliofemoral venous obstruction (IVO) must address engineering challenges distinct from those encountered in arterial stenting. The Grency venous stent, a nitinol self-expanding stent specifically tailored for IVO, features a composite structure designed to meet the stent requirements of various iliac vein segments. The Grency Trial is a prospective, multicenter, single-arm, open-label pivotal study aimed at evaluating the efficacy and safety of the Grency stent system. Following a 12-month follow-up period, the Grency venous stent system has demonstrated both safety and efficacy in treating iliofemoral venous outflow obstruction.

Rocaglaol, embedding a cyclopenta[b]benzofuran scaffold, was isolated mainly from the plants of Aglaiaand exhibited nanomolar level antitumor activity. However, the drug-like properties of these compounds are poor. To improve the physicochemical properties of rocaglaol, 36 nitrogen-containing phenyl-substituted rocaglaol derivatives were designed and synthesized. These derivatives were tested for the inhibitory effects on three tumor cell lines, HEL, MDA-231, and SW480, using the MTT assay. Among them, 22 derivatives exhibited good cytotoxic activities with IC50values between 0.11 ± 0.07 and 0.88 ± 0.02 μM. Fourteen derivatives exhibited stronger cytotoxicity than the positive control, adriamycin. In particular, a water-soluble derivative revealed selective cytotoxic effects on HEL cells (IC50= 0.19 ± 0.01 μM). This compound could induce G1 cell cycle arrest and apoptosis in HEL cells. Western blot assays suggested that the water-soluble derivative could downregulate the expression of the marker proteins of apoptosis, PARP, caspase-3, and caspase-9, thus inducing apoptosis. Further CETSA and Western blot studies implied that this water-soluble derivative might be an inhibitor of friend leukemia integration 1 (Fli-1). This water-soluble derivative may serve as a potential antileukemia agent by suppressing the expression of Fli-1.

Outdoor activities confer mental, physical, and social health benefits to children. In urban areas, parks are vital for children to engage in outdoor activities. Understanding public perceptions and expectations of how urban park environments influence children’s activity could attenuate social and physical inequities and promote park use. Previous studies have used surveys and observational data to analyze preferences for children’s activity in parks. Crowdsourced online data and volunteered geographic information (VGI) could offer a valuable addition to or substitute for traditional approaches. Our study uses social media as a passive data collection method to ascertain public perceptions and expectations about children’s activity in Atlanta parks with a shorter timeframe, compared to a regular survey analysis. We collected 4026 Google Map reviews from 2017 to 2022 that related to children and applied text mining analysis to understand how people perceive children’s activity in Atlanta parks and how perceptions change across different environments. Ten topics were extracted that related to children’s activity in Atlanta parks: attitude, amenity, safety, social, pet, nature, recreational, water sports, water recreational, and sports.The attitude, social,and water recreationaltopics were the most widely discussed topics in all settings. However, in racially diverse and low-crime environments, discussions centered on pet, recreational,and sports,suggest the importance of tailored strategies to promote children’s activities in parks. Park planners and policymakers can use this approach and findings to evaluate and advocate for children’s activities in urban parks. Also, our work helps to expand survey analysis with passive data collection methods from small geographic scales to larger areas and to apply geoanalytics of big data and social media data in investigation research.

To improve hybrid open-endovascular repair, the Viabahn Open Revascularization Technique (VORTEC) has emerged as a sutureless method that reduces renal ischemia time. This study aimed to compare the differences between VORTEC and standard hybrid open-endovascular repair of thoracoabdominal aortic aneurysm (TAAA) in renal ischemia.

Considering the energy consumption and specific performance requirements of mining trucks, a novel uninterrupted multi-speed transmission is proposed in this paper, which is composed of a power-split device, and a three-speed lay-shaft transmission with a traction motor. The power-split device is adapted to enhance the efficiency of the engine by adjusting the gear ratio continuously. The three-speed lay-shaft transmission is designed based on the efficiency map of traction motor to guarantee the drivability. The combination of the power-split device and three-speed lay-shaft transmission can realize uninterrupted gear shifting with the proposed shift strategy, which benefits from the proposed adjunct function by adequately compensating the torque hole. The detailed dynamic models of the system are built to verify the effectiveness of the proposed shift strategy. To evaluate the maximum fuel efficiency that the proposed uninterrupted multi-speed transmission could achieve, dynamic programming is implemented as the baseline. Due to the “dimension curse” of dynamic programming, a real-time control strategy is designed, which can significantly improve the computing efficiency. The simulation results demonstrate that the proposed uninterrupted multi-speed transmission with dynamic programming and real-time control strategy can improve fuel efficiency by 11.63% and 8.51% compared with conventional automated manual transmission system, respectively.

Given that hybrid electric vehicles could significantly reduce fuel consumption, and mining trucks are much more sensitive to energy conversion efficiency, it is essential to develop a hybrid system, which could meet both the specific requirements of the structure of mining trucks and the needs for overall efficiency improvements. In this paper, a novel multi-mode transmission system, which is composed of an engine, two motors, one planetary gear set, and a parallel transmission structure, is proposed. The planetary gear set serves as a power-split device, and the parallel transmission structure can make the engine and motor work in the high-efficiency area both in low-speed and high-speed conditions. Due to the flexibility of the proposed system, five power-flow modes can be implemented, and they are illustrated with a specially designed rule-based energy management strategy. To validate the effectiveness of the multi-mode transmission system, dynamic programming is used to assess the fuel consumption during the given driving cycle without considering drivability. The simulation results reveal that the proposed multi-mode transmission system owns better fuel efficiency for mining trucks compared with highly regarded Toyota Hybrid System and conventional automated manual transmission system.

The articulated frame-steered vehicles (AFSV) exhibit enhanced maneuverability but reduced yaw stability and greater steering power consumption. Apart from kinematics of the steering system, the dynamics of the actuating system strongly influence the performance of the AFSV, which is generally neglected in the reported studies. In this study, a yaw-plane model of the articulated vehicle coupled with the kinematic and dynamics properties of the steering struts is formulated to identify objective measures of the AFSV under steering inputs. The results suggest that the vehicle yaw oscillation/stability, steering power efficiency and maneuverability can be objectively measured in terms of the strut length, yaw oscillation frequency and damping ratio, steering gain, and steering response rate and overshoot. The layout of steering struts and properties of the steering valve and hydraulic fluid are optimized while employing the weighted-sum method and a combination of pattern search and sequential quadratic programming algorithms. The relative weights of individual performance measures were obtained using the analytic hierarchy process (AHP) model. The solutions of the optimization problem revealed more compact articulated frame steering (AFS) system design with over 20% reduction in strut length and 24% gain in the yaw oscillation frequency. Increasing the fluid bulk modulus resulted in more compact AFS layout and further increase in the yaw oscillation frequency with lower response overshoot. The optimal design based on weighted sum of various performance measures, however, revealed negligible changes in terms of the steering power efficiency.

Angiitis‐induced critical limb ischemia (AICLI) patients constitute a remarkable proportion of no‐option critical limb ischemia (CLI) patients. Stem cell therapy has become an innovative and promising option for no‐option CLI patients. As one of these promising stem cell therapies, purified CD34+ cell transplantation (PuCeT) has shown favorable short‐term results. However, the long‐term efficacy of PuCeT has yet to be reported. This study evaluates the long‐term efficacy of PuCeT in AICLI patients. Twenty‐seven AICLI patients were enrolled from May 2009 to December 2011. Granulocyte colony‐stimulating factor (G‐CSF) and enoxaparin sodium were administered for 5 days. On day 5, CD34+ cell isolation was performed, and cells were transplanted by intramuscular injection. The primary endpoint, major‐amputation‐free survival rate (MAFS), as well as secondary endpoints, such as peak pain‐free walking time (PPFWT) and the Wong‐Baker FACES pain rating scale score (WFPRSS), were routinely evaluated during the 5‐year follow‐up period. The endpoints were as follows: the MAFS was 88.89%; PPFWT increased from 3 ± 3 to 17 ± 6 minutes; WFPRSS decreased from 7 ± 2 to 0.3 ± 1.7; the ulcer healing rate was 85.71%; the recurrence rate was 11.11%; and SF‐36v2 scores were significantly improved at 5 years after PuCeT. The rate of labor recovery 5 years after PuCeT was 65.38%, and no severe adverse effect was observed during the treatment. PuCeT demonstrated long‐term efficacy and durability as a treatment of AICLI not only in achieving limb salvage but also in recovering the labor competence and improving the quality of life of patients. StemCellsTranslationalMedicine2018;7:583–590 Figure A, B, and C (before therapy, 3 months, and 6 months after therapy) show the ulcer healing process of a critical limb ischemia patient with Burger's disease and the efficacy of purified CD34+ cell therapy for angiitis‐induced no‐option critical limb ischemia.

The directional performance characteristics of articulated frame steering vehicles are known to be strongly coupled with the kinematic characteristics and the dynamic characteristics of the steering system. The reported studies on articulated frame steering generally focus on the yaw divergence behaviour or the snaking tendency of the vehicle based on its free-yaw-oscillation response, while neglecting important contributions due to the kinematics and the dynamics of the steering system. This study characterizes both the free-yaw-oscillation response and the transient steering response of an articulated frame steering mining vehicle, considering the kinematics of the steering struts together with the dynamics of the flow volume-regulated steering valve and the actuating system. The validity of the analytical vehicle and steering system model is demonstrated using the measured data acquired for the vehicle. The free-oscillation behaviour of the articulated frame steering is characterized in terms of the yaw-mode natural frequency and the yaw damping ratio. The transient responses of the articulated frame steering are assessed in terms of the steering gain, the rate of articulation and the articulation overshoot. The effects of the variations in the various articulated frame steering parameters on the free response and the transient response are subsequently investigated and discussed so as to seek guidance for the design of the articulated frame steering system. It is shown that a higher bulk modulus of the hydraulic fluid and longer steering-arm lengths yield a higher yaw stiffness of the articulated frame steering system and thereby a higher frequency of the yaw oscillations. Higher leakage flows and higher viscous seal friction cause a higher yaw damping coefficient but decrease the steering gain and the articulation rate of the vehicle.

This paper presents an overview of various innovative fabrication approaches and the potential applications of ionic polymer metal composites (IPMC), which is a composite material consisting of a polymer membrane sandwiched between two thin electrode layers. When given a voltage within a range of 1-5V, cations inside accompanying with water molecules of IPMC move across the width of the material causing a uniform water distribution and finally to achieve it’s bending motion. In addition to a classical processing method, three innovative modification approaches are recommended to fabricate IPMC, particularly to settle water electrolysis and leakage for multiple practical applications. Also, three applications are extensively highlighted in the later pages of the paper. This is a very new field and with the research done so far, it is believed that IPMC has a potential which is worth research elaborately. This paper presents an overview of the manufacturing components, techniques, related problems and applications of IPMC. Additionally, it recommends innovative modification fabrication approaches to subdue the associated problems in the existing conventional fabrication processing.

Vaccination is an effective mean of preventing infectious diseases, including those caused by Helicobacter pylori. Th17 cell responses are critical for the pathogenesis of Helicobacter pyloriinfection. In view of Th17 responses to multi-epitope vaccine CTB-UE, the IL-17 production in antiserum was examined. CTB-UE immunization decreased IL-17 production, implying that Th17 responses may be inhibited. Furthermore, IL-17 aggravated GES-1 cell injury induced by H. pylori SS1;In contrast, CTB-UE antiserum could alleviate this cell injury, which suggesting that CTB-UE can protect GES-1 cell infected with H. pylori SS1by inhibiting Th17 responses. Treatment of mice with CTB-UE significantly reduced the H. pyloriburden and inflammation in the stomach. On the other hand, the production of IL-17 in the stomach in H. pylori-infected mice was increased; but the production of IL-17 in the stomach was decreased after treatment with CTB-UE. Furthermore, the expression of microRNA-155 in gastric tissue was significantly up-regulated. The results suggested that CTB-UE could relieve the H. pylori-induced gastric inflammatory reaction via up-regulating microRNA-155 to inhibit Th17 responses, implying that the microRNA-155/IL-17 pathway was involved. Further study is required to elucidate the relationship between miRNA-155 and IL-17. We found that the production of IL-17 was significantly increased after the expression of miRNA-155 being down-regulated; however, the production of IL-17 was significantly decreased after the expression of miRNA-155 being upregulated.

When the wheel of the wheel edges reducer working, single tooth meshing and bidentate meshing alternately, Gear meshing dislocation, mutation load, speed fluctuations, engaging-in and engaging-out impact will inevitably appear. This paper take the wheel hub reducer based on 60t AC drive articulated dump truck as the research object, use romaxdesigner model and motion simulation analyze the wheel edges reducer. through the analysis obtain the stress and the longevity of gear. According to the simulation results correct the gear for the purpose of improving the bearing capacity of the gear and reducing meshing noise.

Axial flow fans are widely used in our daily lives such as automotive cooling systems, electronic appliances and air conditioning. The factors that affect the aerodynamic performance of axial flow fans include blade number, fan diameter, hub ratio, blade angle, rotating speed, etc. This paper investigates effects of hub ratio and bladenumber on the aerodynamic performance of the axial flow fan of the radiator of a dump truck we previously designed for mining. With the computational fluid dynamics (CFD) method, the fans operation condition, such as entry static pressure, effective power and flow rate, are numerically simulated with various hub ratios and blade numbers. The results of the numerical simulations are quantitatively analyzed and curves of fans performance changing with hub ratio and blade number are given. An optimization design for the cooling fan is conducted. The results of numerical simulation give conclusion that an increase of 5.6% of the vacuum static pressure and an increase of 3% of flow rate can be obtained when the hub ratio is between 500-600mm, with the same or even less power consumption. The flow rate increment reaches its peak at 8%, while power consumption efficiency is almost linearly increased, when the blade number increases to be in the range of 8 to 10. That is to say when the blade number is between 8 to 10, the fan is apparently making better use of energy. Compared with experimental method, computational fluid dynamics (CFD) method can reduce design circle and lower cost. The CFD results provide guidance to improvement of design efficiency and energy utilization.

n this paper we propose a intelligent vehicle system design with MK60N512VMD100 as the micro-controller unit and CMOS image sensor to obtain image information of the track. Our proposed comparator circuit instead of an A/D conversion to obtain the binary image enables us to achieve a bigger field of view and a higher processing speed. A unique algorithm to extract track information and to successfully extract the black guide line is devised. We also design a PID closed-loop control to adjust the rotate speed of driving electromotor and direction of steering electromotor. The results indicate that our design proposal is feasible. The intelligent vehicle could successfully achieve a high speed, while maintaining an excellent stability.

A method is proposed to predict the handling and roll properties of electric drive mining trucks and evaluate their safety status. Trucksim is used to simulate the truck dynamics and Simulink is used to model the electric drive system, the truck model takes a double-lane change manoeuvre according to ISO 3888-1. A 190t mining truck is demonstrated and the understeer gradient and roll angle results respect its safety status well. Using this method, mining truck designers could make the handling and roll prediction before manufacture and modify their design.

Lung cancer is one of the most death-related cancers worldwide. Ursolic acid (UA), a pentacyclic triterpene acid, has a wide range of anticancer functions such as proapoptosis, antiangiogenesis, and antimetastasis. This study was carried out to explore the inhibition mechanism of UA on metastasis of lung cancer A549 cells. First, we found that UA inhibited the metastasis of lung cancer cells in a concentration-dependent manner through an adhesion assay, a cell wound healing assay, and a transwell migration assay in vitro. In addition, after treatment with UA, the A549 cells showed decreased expression of astrocyte-elevated gene-1 (AEG-1) accompanied by upregulation of E-cadherin and downregulation of N-cadherin and vimentin, which have been reported to characterize the epithelial–mesenchymal transition (EMT). Further results also confirmed that the expression of vimentin was decreased by the siRNA technique to directly knock down AEG-1 expression, indicating that AEG-1 was involved in UA-mediated EMT inhibition. Furthermore, our results showed that UA suppressed the expression level of AEG-1 by repressing nuclear factor-B signaling. Altogether, UA inhibited the EMT by suppressing the expression of AEG-1, correlating with inhibition of nuclear factor-B in A549 cells. These findings suggested that UA was a potent anti-lung cancer agent, and it may be able to prevent invasion and metastasis of lung cancer cells.

During the frame optimization design, according to fuzzy integrated evaluation theory, fuzzy mathematical models of intensity coefficient and mass coefficient and intensity-mass ratio coefficient of dump truck frame were established. Based on those, in terms of Sugeno fuzzy discursion method, fuzzy discursion model about frame intensity-mass relationship rationality was established, too. Through checkout of three dump truck frames of 170t and 85t and 42t, validation results were the same as the fact. So, it was concluded that the fuzzy integrated evaluation model and the fuzzy discursion model gave helpful guidance on frame designing work.

The rear multi-stage cylinder direct-acting hydraulic body hoist mechanism is widely used in mining dump truck. The working principle of the body hoist mechanism is introduced. The dumping process is simplified through the introduction of “dumping line” and the variation of ore mass and hoist torque are analyzed. The optimization design of the position of body hoist mechanism is done using Maple software on the basis of direct search method aimed at minimizing the initial hoist force and the stroke of cylinder and maximizing the hoist force arm when the second stage cylinder starts to stretch out.

In the paper, analysis and comparison is made for different optimization design methods of the separation steering trapezoidal mechanism based on the steering system of 170 tons mining dump truck. The optimal design method is obtained, and research shows that the steering mechanism analysis method introduced in many automotive design textbooks has errors and should be improved.

A test platform based on hydro-pneumatic suspension was set up to research the influence of temperature for the hydro-pneumatic suspension system. A series of experiments on damping holes of different diameters were carried out, and the temperature of oil and pressure on both sides of damping holes were recorded. A mathematical model of this platform was established using knowledge of fluid mechanics. The result of mathematical model was verified by experiments result, and the result is that oil temperature has little influence to working condition of suspension cylinder when it is between room temperature and 75℃.

According to design ideas of the frame of the 170 tons dump truck, and allaying effects on the frame carling from the lift oil cylinder support (LOCS), the lift oil cylinder support of float support structure (LOCSFSS) is designed by optimization technology. Then, hydraulic pressure lifting system is emulated by one-dimensional emulation, and the load curve is gotten during lifting. Based on the load curve, LOCSFSS and the frame carling are all analyzed by explicit dynamics. Afterward, contrasts and analysis are done among float support structure and the other two traditional structures of jointing on bottom steel board structure (JBSBS) and carling structure (CS). Results of that indicate LOCSFSS is obviously better than the other two structures.

This paper studied misfire diagnosis of diesel engine based on short-time vibration characters. Misfire of diesel engine was simulated by the vibration monitoring test. Cylinder vibration signal and top center signal were collected under different states. The short-time vibration signal of each cylinder was intercepted according to the diesel combustion sequence, effective value was calculated, and BP Neural Network model built with this character was used to diagnose diesel misfire. The result shows that this method can locate the misfire cylinder effectively, and it is meaningful for guiding the detection and repair of vehicles.

Six novel Maillard reaction products (MRPs) (1–6) were isolated from the processed Thermopsis lanceolataR. Br. seed extract, along with one biogenetically related intermediate (7). Compounds 1–4possessed three rare dimerization patterns constructed by cytisine, whereas compounds 5and 6represented the first example of the addition products of cytisine and 5,6-dihydroxy-4-hexanolide. Their structures were elucidated by comprehensive spectroscopic data analysis and quantum chemistry calculations including GIAO 13C{1H} NMR and ECD calculation, combined with single-crystal X-ray diffraction analysis. Biologically, compound 3displayed significant anti-tobacco mosaic virus activity compared with the positive control ningnanmycin.

The stable and homogeneneous aqueous suspension of carbon nanotubes was prepared in this study. The stability of the nanofluids was improved greatly due to the use of a new dispersant, humic acid. The thermal conductivity of the aqueous suspension was measured with the 3ω method. The experimental results showed that the thermal conductivity of the suspensions increases with the temperature and also is nearly proportional to the loading of the nanoparticles. The thermal conductivity enhancement of single-walled carbon nanotubes (SWNTs) suspensions is better than that of the multi-walled carbon nanotubes (MWNTs) suspensions. Especially for a volume fraction of 0.3846% SWNTs, the thermal conductivity is enhanced by 40.5%. Furthermore, the results at 30°C match well with Jang and Choi’s model.

Purpose: To evaluate the feasibility and efficacy of a vascular occluder to treat a patent re-entry tear near a visceral artery after stent-graft repair of Stanford type B aortic dissection.Case Report: A 34-year-old woman with a history of stent-graft repair for aortic dissection 6 months ago was admitted complaining of recurrent chest discomfort for 1 month. Computed tomographic angiography (CTA) revealed a proximal type I endoleak and a patent re-entry tear above the celiac artery orifice. A double-disk vascular occluder was used to treat the re-entry tear. The device was deployed successfully without perioperative complications; the re-entry tear was closed and perfusion of adjacent vessels was not compromised. CTA at 3 months and 1 year documented thrombus formation in the false lumen, proper position of the occluder, and no re-entry tear.Conclusion: It is feasible to treat patent re-entry tears with a vascular occluder after primary proximal stent-graft repairs. Long-term clinical efficacy has yet to be confirmed.

The thermal performance of a nuclear reactor core contains various engineering uncertainties. These uncertainties often arise from calculation, measurement, instrumentation, manufacture, fabrication and data processing. Statistical techniques are useful to evaluate and combine these uncertainties in the thermal design of nuclear reactors. In this paper, a statistical method is developed and employed in the thermal design of the supercritical pressure light water reactor (Super LWR) to evaluate the statistical engineering uncertainties. This method is referred as the Monte Carlo Statistical Thermal Design Procedure for Super LWR (MCSTDP). This method uses the maximum cladding surface temperature (MCST) as a crucial criterion and a sub-channel code is utilized to perform the core thermal hydraulic analysis. The engineering uncertainties are considered with strict respect to the 95/95 limit of Super LWR. The main purpose of this paper is to establish the statistical evaluation methodology. The engineering uncertain for the thermal design of Super LWR is evaluated by using this method to get an approximate quantification. The results are compared with those of the Revised Thermal Design Procedure (RTDP) of Super LWR.

ABSTRACTThe acquisition of immunity following subclinical or resolved infection with the intracellular parasite Leishmania donovanisuggests that vaccination could prevent visceral leishmaniasis (VL). The LACK (Leishmaniahomolog of receptors for activated C kinase) antigen is of interest as a vaccine candidate for the leishmaniases because of its immunopathogenic role in murine L. majorinfection. Immunization of mice with a truncated (24-kDa) version of the 36-kDa LACK antigen, delivered in either protein or DNA form, was found previously to protect against cutaneous L. majorinfection by redirecting the early T-cell response away from a pathogenic interleukin-4 (IL-4) response and toward a protective Th1 response. The amino acid sequence of theLeishmaniap36(LACK) antigen is highly conserved, but the efficacy of this vaccine antigen in preventing disease caused by strains other than L. majorhas not been determined. We investigated the efficacy of a p36(LACK) DNA vaccine against VL because of the serious nature of this form of leishmaniasis and because it was unclear whether the LACK vaccine would be effective in a model where there was not a dominant pathogenic IL-4 response. We demonstrate here that although the LACK DNA vaccine induced a robust parasite-specific Th1 immune response (IFN-γ but not IL-4 production) and primed for an in vivo T-cell response to inoculated parasites, it did not induce protection against cutaneous or systemic L. donovanichallenge. Coadministration of IL-12 DNA with the vaccine did not enhance the strong vaccine-induced Th1 response or augment a protective effect.

ABSTRACTMalnutrition is a risk factor for the development of visceral leishmaniasis. However, the immunological basis for this susceptibility is unknown. We have developed a mouse model to study the effect of malnutrition on innate immunity and early visceralization following Leishmania donovaniinfection. Three deficient diets were studied, including 6, 3, or 1% protein; these diets were also deficient in iron, zinc, and calories. The control diet contained 17% protein, was zinc and iron sufficient, and was provided ab libitum. Three days after infection with L. donovanipromastigotes, the total extradermal (lymph nodes, liver, and spleen) and skin parasite burdens were equivalent in the malnourished (3% protein) and control mice, but in the malnourished group, a greater percentage (39.8 and 4.0%, respectively;P= 0.009) of the extradermal parasite burden was contained in the spleen and liver. The comparable levels of parasites in the footpads in the two diet groups and the higher lymph node parasite burdens in the well-nourished mice indicated that the higher visceral parasite burdens in the malnourished mice were not due to a deficit in local parasite killing but to a failure of lymph node barrier function. Lymph node cells from the malnourished, infected mice produced increased levels of prostaglandin E2(PGE2) and decreased levels of interleukin-10. Inducible nitric oxide synthase activity was significantly lower in the spleen and liver of the malnourished mice. Thus, malnutrition causes a failure of lymph node barrier function after L. donovaniinfection, which may be related to excessive production of PGE2and decreased levels of IL-10 and nitric oxide.

An hybrid uninterrupted multi-speed transmission (HUMST), based on the integration of a planetary gear set and a 3-speed automatic manual transmission (3-AMT), is developed to satisfy the specific performance indexes of mining trucks. The power-split device can alleviate and eliminate the inherent torque interruption of the 3-AMT during gear shift by implementing the designed cooperative shift control strategy which is optimized by quadratic performance index. In order to achieve fast torque coordination while guaranteeing the driving comfort performance, the torque profiles of the power split device and the traction motor are optimized by Linear-quadratic regulator (LQR) algorithm. Dynamic programming (DP) is implemented as a benchmark to demonstrate the maximum fuel efficiency of the proposed HUMST. Because of the high computational cost of optimal control strategies such as DP, an improved real-time control strategy (IRTCS) using modified Gaussian distribution function is proposed to significantly reduce the computing load. As efficiency-oriented energy control strategy would result in frequent gear shifts, to achieve a desirable tradeoff between the overall efficiency and the shift stability, multi-objective genetic algorithm (MGA) is integrated to optimize the overall performance. The detail mathematical and dynamic model shows that the proposed shifting strategy with LQR can effectively suppress shift jerk, and the proposed IRTCS with MGA can reduce shift frequency by 70.78% to improve the drivability, only sacrificing 4.86% of overall efficiency compared to that of DP. [ABSTRACT FROM AUTHOR]

To achieve the goal of driver-less underground mining truck, a fuzzy hyperbolic tangent model is established for path tracking on an underground articulated mining truck. Firstly, the sample data of parameters are collected by the driver controlling articulated vehicle at a speed of 3 m/s, including both the lateral position deviation and the variation of heading angle deviation. Then, according to the improved adaptive BP neural network model and deriving formula of mediation rate of error estimator by the method of Cauchy robust, the weights are identified. Finally, H-infinity control controller is designed to control steering angle. The results of hardware-in-the-loop simulation show that lateral position deviation, heading angle deviation, and steering angle of the vehicle can be controlled, respectively, at 0.024 m, 0.08 rad, and 0.21 rad. All the deviations are asymptotically stable, and error control is in less than 2%. The method is demonstrated to be effective and reliable in path tracking for the underground vehicles.