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The probiotic Bacillus subtilis(B. subtilis) was widely applied in animal production as feed additive. Lysine (Lys) and methionine (Met) were the two most important limiting amino acids in livestock animal feed. Raising Lys and Met contents in B. subtiliswould provide better effects for animal production and save Lys and Met supplements. We still didn't know whether Lys-rich and Met-rich protein genes from plants could be transfected into B. subtilisand expressed at a high level so as to improve animal production, such as milk production as an additional diet. The Lys-rich protein gene (Cflr) and Met-rich 10 ku-δ Zeinwere cloned from pepper anther and maize endosperm, respectively. Then they were constructed into plasmids individually and successfully cotransfected into B. subtilis.Upon IPTG induction, mRNAs and protein expressions could be observed. Lys and Met contents in the fermentation broth were raised by 65.92% and 46.39%, respectively. After feeding 200 g and 400 g · cow−1· d−1, transgenic B. subtilisfermentation broth, the milk yield, milk protein and milk fat contents all significantly increased. The Lys-rich protein gene (Cflr) and Met-rich 10 ku-δ Zeinwere successfully transfected into B. subtilis.Contents of Lys and Met in the transgenic B. subtilisobviously raised and the fermentation broth of the transgenic bacteria could effectively improve milk yield and quality.

The use of genetically modified organisms (GMOs) as food products becomes more and more widespread. The European Union has implemented a set of very strict procedures for the approval to grow, import and/or utilize GMOs as food or food ingredients. Thus, analytical methods for detection of GMOs are necessary in order to verify compliance with labelling requirements. There are few effective screening methods for processed GM (genetically modified) products. Three anti-herbicide genes (CP4-EPSPS, BARand PAT) are common exogenous genes used in commercialized transgenic soybean, maize and rice. In the present study, a new SYBR®Green qPCR screening method was developed to simultaneously detect the three exogenous anti-herbicide genes and one endogenous gene in a run. We tested seven samples of representative processed products (soya lecithin, soya protein powder, chocolate beverage, infant rice cereal, maize protein powder, maize starch, and maize jam) using the developed method, and amplicons of endogenous gene and transgenic fragments were obtained from all the processed products, and the sensitivity was 0.1%. These results indicated that SYBR®Green qPCR screening method was appropriate for qualitative detection of transgenic soybean, maize and rice in processed products.