17 results on '"Xia, LiMin"'
Search Results
2. Targeting MMP9 in CTNNB1 mutant hepatocellular carcinoma restores CD8+T cell-mediated antitumour immunity and improves anti-PD-1 efficacy
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Cai, Ning, Cheng, Kun, Ma, Yue, Liu, Sha, Tao, Ran, Li, Yani, Li, Danfeng, Guo, Bin, Jia, Wenlong, Liang, Huifang, Zhao, Jianping, Xia, Limin, Ding, Ze-yang, Chen, Jinhong, and Zhang, Wanguang
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ObjectiveThe gain of function (GOF) CTNNB1 mutations (CTNNB1GOF) in hepatocellular carcinoma (HCC) cause significant immune escape and resistance to anti-PD-1. Here, we aimed to investigate the mechanism of CTNNB1GOFHCC-mediated immune escape and raise a new therapeutic strategy to enhance anti-PD-1 efficacy in HCC.DesignRNA sequencing was performed to identify the key downstream genes of CTNNB1GOFassociated with immune escape. An in vitro coculture system, murine subcutaneous or orthotopic models, spontaneously tumourigenic models in conditional gene-knock-out mice and flow cytometry were used to explore the biological function of matrix metallopeptidase 9 (MMP9) in tumour progression and immune escape. Single-cell RNA sequencing and proteomics were used to gain insight into the underlying mechanisms of MMP9.ResultsMMP9 was significantly upregulated in CTNNB1GOFHCC. MMP9 suppressed infiltration and cytotoxicity of CD8+T cells, which was critical for CTNNB1GOFto drive the suppressive tumour immune microenvironment (TIME) and anti-PD-1 resistance. Mechanistically, CTNNB1GOFdownregulated sirtuin 2 (SIRT2), resulting in promotion of β-catenin/lysine demethylase 4D (KDM4D) complex formation that fostered the transcriptional activation of MMP9. The secretion of MMP9 from HCC mediated slingshot protein phosphatase 1 (SSH1) shedding from CD8+T cells, leading to the inhibition of C-X-C motif chemokine receptor 3 (CXCR3)-mediated intracellular of G protein-coupled receptors signalling. Additionally, MMP9 blockade remodelled the TIME and potentiated the sensitivity of anti-PD-1 therapy in HCC.ConclusionsCTNNB1GOFinduces a suppressive TIME by activating secretion of MMP9. Targeting MMP9 reshapes TIME and potentiates anti-PD-1 efficacy in CTNNB1GOFHCC.
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- 2024
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3. High-throughput and proteome-wide discovery of endogenous biomolecular condensates
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Li, Pengjie, Chen, Peng, Qi, Fukang, Shi, Jinyun, Zhu, Wenjie, Li, Jiashuo, Zhang, Peng, Xie, Han, Li, Lina, Lei, Mengcheng, Ren, Xueqing, Wang, Wenhui, Zhang, Liang, Xiang, Xufu, Zhang, Yiwei, Gao, Zhaolong, Feng, Xiaojun, Du, Wei, Liu, Xin, Xia, Limin, Liu, Bi-Feng, and Li, Yiwei
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Phase separation inside mammalian cells regulates the formation of the biomolecular condensates that are related to gene expression, signalling, development and disease. However, a large population of endogenous condensates and their candidate phase-separating proteins have yet to be discovered in a quantitative and high-throughput manner. Here we demonstrate that endogenously expressed biomolecular condensates can be identified across a cell’s proteome by sorting proteins across varying oligomeric states. We employ volumetric compression to modulate the concentrations of intracellular proteins and the degree of crowdedness, which are physical regulators of cellular biomolecular condensates. The changes in degree of the partition of proteins into condensates or phase separation led to varying oligomeric states of the proteins, which can be detected by coupling density gradient ultracentrifugation and quantitative mass spectrometry. In total, we identified 1,518 endogenous condensate proteins, of which 538 have not been reported before. Furthermore, we demonstrate that our strategy can identify condensate proteins that respond to specific biological processes.
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- 2024
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4. Unsupervised learning of local features for person re-identification with loss function
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Tan, Lunzheng, Chen, Guoluan, Ding, Rui, and Xia, Limin
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Many methods for person re-identification focus on making full use of local features, which typically requires either comprehensive manual labelling or complex pretreatment. This paper proposes a novel loss function, termed feature channels dropout and de-similarity loss that drives the autonomous learning of discriminative local features in convolutional neural networks. The proposed loss function consists of two components. The first is a feature channels dropout component designed to compel each feature channel to be discriminative. A novel channel-dropout function and a cross-channel-element-max function are applied in this component. The second component is a de-similarity component that uses the Pearson correlation coefficient to constrain feature channels and ensure they differ from each other. This component is conducive to diverse local features in mining. Extensive experiments on three large-scale re-identification datasets demonstrate that feature channels dropout and de-similarity loss achieve superior performance compared with state-of-the-art methods.
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- 2023
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5. T-box transcription factor 19 promotes hepatocellular carcinoma metastasis through upregulating EGFR and RAC1
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Ji, Xiaoyu, Chen, Xiaoping, Zhang, Bixiang, Xie, Meng, Zhang, Tongyue, Luo, Xiangyuan, Liu, Danfei, Feng, Yangyang, Wang, Yijun, Sun, Mengyu, Li, Congxin, Huang, Wenjie, and Xia, Limin
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The effect of targeted therapy for metastatic hepatocellular carcinoma (HCC) is still unsatisfactory. Exploring the underlying mechanism of HCC metastasis is favorable to provide new therapeutic strategies. T-box (TBX) transcription factor family genes, which are crucial regulators in embryo and organ development, are vital for regulating tumor initiation, growth and metastasis. Here we explored the role of TBX19 in HCC metastasis, which is one of the most upregulated TBX family genes in human HCC tissues. TBX19 expression was markedly upregulated in HCC tissues and elevated TBX19 expression predicted poor prognosis. Overexpression of TBX19 enhanced HCC metastasis through upregulating epidermal growth factor receptor (EGFR) and Rac family small GTPase 1 (RAC1) expression. Downregulation of EGFR and RAC1 inhibited TBX19-mediated HCC metastasis, while upregulation of EGFR and RAC1 restored inhibition of HCC metastasis mediated by TBX19 knockdown. Furthermore, epidermal growth factor (EGF)/EGFR signaling upregulated TBX19 expression via the extracellular signal-regulated kinase (ERK)/nuclear factor (NF)-kB axis. Besides, the combined application of EGFR inhibitor Erlotinib and RAC1 inhibitor NSC23766 markedly inhibited TBX19-mediated HCC metastasis. In HCC cohorts, TBX19 expression was positively associated with EGFR and RAC1 expression. Patients with positive coexpression of TBX19/EGFR or TBX19/RAC1 displayed the poorest prognosis. In conclusion, EGF/EGFR signaling upregulated TBX19 expression via ERK/NF-kB pathway and TBX19 fostered HCC metastasis by enhancing EGFR and RAC1 expression, which formed an EGF-TBX19-EGFR positive feedback loop. Targeting this signaling pathway may offer a potential therapeutic strategy to efficiently restrain TBX19-mediated HCC metastasis.
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- 2022
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6. Association between CYP2C9, VORC1, VDR,and APOEgenotypes on warfarin maintenance and response during initial anticoagulation for Chinese patients with heart valve replacement
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Li, Xiaoye, Wang, Zi, Liu, Zhiyan, Xia, Limin, Lyu, Qianzhou, and Wang, Ningning
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- 2022
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7. The roles of nausea and vomiting in COVID-19: did we miss something?
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Zhang, Tongyue, Liu, Danfei, Tian, Dean, and Xia, Limin
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The Coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is an international public health emergency. Although respiratory symptoms predominate the clinical manifestations of COVID-19, gastrointestinal symptoms have been observed in a subset of patients. Notably, some patients have nausea/vomiting as the first clinical manifestation of COVID-19, which is often overlooked by people. It is now clear that not only the lungs, the gastrointestinal tract could also be attacked by SARS-CoV-2. Its host receptor angiotensin-converting enzyme 2 (ACE2), which acts as a gateway to infection, has been found to be highly expressed in the gastrointestinal epithelium and may lead to the development of nausea/vomiting. Raise awareness of these symptoms and take timely intervention would help people combat the pandemic. This review discussed epidemiology, mechanisms, management, and prevention of COVID-19 related nausea and vomiting.
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- 2021
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8. Pan-cancer analysis of ABCC1 as a potential prognostic and immunological biomarker
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Wang, Tiantian, Rao, Dean, Fu, Chenan, Luo, Yiming, Lu, Junli, Liang, Huifang, Xia, Limin, and Huang, Wenjie
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•ABCC1 expression exhibited intricate associations with diverse immune-related genes.•ScRNA-seq analysis from GEO revealed a positive correlation between ABCC1 expression and macrophage infiltration.•Various vitro and vivo experiments substantiated the oncogenic role of ABCC1 in hepatocellular carcinoma.
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- 2024
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9. SIX4 promotes hepatocellular carcinoma metastasis through upregulating YAP1 and c-MET
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He, Qin, Lin, Zhuoying, Wang, Zhihui, Huang, Wenjie, Tian, Dean, Liu, Mei, and Xia, Limin
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Metastasis is the main reason for high mortality in hepatocellular carcinoma (HCC) patients and the molecular mechanism remains unclear. Therefore, it is important to elucidate the mechanism underlying HCC metastasis. Here, we report a novel role of SIX homeobox 4 (SIX4), one of the SIX gene family, in promoting HCC metastasis. The elevated expression of SIX4 was positively correlated with loss of tumor encapsulation, microvascular invasion, higher TNM stage, and poor prognosis in human HCC. SIX4 expression was an independent and significant risk factor for the recurrence and survival in HCC patients. Upregulation of SIX4 promoted HCC invasion and metastasis, whereas downregulation of SIX4 decreased HCC invasion and metastasis. SIX4 transactivated Yes1 associated transcriptional regulator (YAP1) and MET proto-oncogene, receptor tyrosine kinase (MET) expression through directly binding to their promoters. Knockdown of YAP1 and c-MET inhibited SIX4-medicated HCC metastasis, while the stable overexpression of YAP1 and c-MET reversed the decreased metastasis induced by SIX4 knockdown. Hepatocyte growth factor (HGF), the specific ligand of c-MET, upregulated SIX4 expression through ERK/NF-?B pathway. Knockdown of SIX4 significantly decreased HGF-enhanced HCC metastasis. In human HCC tissues, SIX4 expression was positively correlated with nuclear YAP1, c-MET and HGF expression. Patients with positive coexpression of SIX4/ nuclear YAP1, SIX4/c-MET or HGF/SIX4 had the poorest prognosis. Moreover, the combination treatment of YAP1 inhibitor Verteporfin and c-MET inhibitor Capmatinib significantly suppressed SIX4-mediated HCC metastasis. In conclusion, SIX4 is a prognostic biomarker in HCC patients and targeting the HGF-SIX4-c-MET positive feedback loop may provide a promising strategy for the treatment of SIX4-driven HCC metastasis.
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- 2020
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10. SOX18 promotes gastric cancer metastasis through transactivating MCAM and CCL7
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Chen, Jie, Dang, Yunzhi, Feng, Weibo, Qiao, Chenyang, Liu, Danfei, Zhang, Tongyue, Wang, Yijun, Tian, Dean, Fan, Daiming, Nie, Yongzhan, Wu, Kaichun, and Xia, Limin
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The therapeutic strategies for advanced gastric cancer (GC) remain unsatisfying and limited. Therefore, it is still imperative to fully elucidate the mechanisms underlying GC metastasis. Here, we report a novel role of SRY-box transcription factor 18 (SOX18), a member of the SOX family, in promoting GC metastasis. The elevated expression of SOX18 was positively correlated with distant metastasis, higher AJCC stage, and poor prognosis in human GC. SOX18 expression was an independent and significant risk factor for the recurrence and survival in GC patients. Up-regulation of SOX18 promoted GC invasion and metastasis, whereas down-regulation of SOX18 decreased GC invasion and metastasis. Melanoma cell adhesion molecule (MCAM) and C-C motif chemokine ligand 7 (CCL7) are direct transcriptional targets of SOX18. Knockdown of MCAM and CCL7 significantly decreased SOX18-mediated GC invasion and metastasis, while the stable overexpression of MCAM and CCL7 reversed the decrease in cell invasion and metastasis that was induced by the inhibition of SOX18. A mechanistic investigation indicated that the upregulation of SOX18 that was mediated by the CCL7-CCR1 pathway relied on the ERK/ELK1 pathway. SOX18 knockdown significantly reduced CCL7-enhanced GC invasion and metastasis. Furthermore, BX471, a specific CCR1 inhibitor, significantly reduced the SOX18-mediated GC invasion and metastasis. In human GC tissues, SOX18 expression was positively correlated with CCL7 and MCAM expression, and patients with positive coexpression of SOX18/CCL7 or SOX18/MCAM had the worst prognosis. In conclusion, we defined a CCL7-CCR1-SOX18 positive feedback loop that played a pivotal role in GC metastasis, and targeting this pathway may be a promising therapeutic option for the clinical management of GC.
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- 2020
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11. SPOCK1 overexpression induced by platelet-derived growth factor-BB promotes hepatic stellate cell activation and liver fibrosis through the integrin α5β1/PI3K/Akt signaling pathway
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Du, Zhipeng, Lin, Zhuoying, Wang, Zhihui, Liu, Danfei, Tian, Dean, and Xia, Limin
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Sparc/osteonectin, cwcv, and kazal-like domain proteoglycan 1 (SPOCK1) is a matricellular protein which regulates cell proliferation, invasion, and survival but the function of SPOCK1 in liver fibrosis is obscure. In this study, we found that SPOCK1 expression increased significantly in fibrotic liver tissues and activated primary rat hepatic stellate cells (R-HSCs). SPOCK1 co-localized with α-smooth muscle actin (α-SMA) in the cytoplasm. Mechanistically, we found platelet-derived growth factor-BB (PDGF-BB) induced SPOCK1 expression by activating the PI3K/Akt/forkhead box M1 (FoxM1) signaling pathway. Intracellular SPOCK1 downregulation decreased the HSC activation, proliferation, and migration induced by PDGF-BB. Furthermore, intracellular SPOCK1 overexpression or recombinant SPOCK1 treatment promoted HSC activation, proliferation, and migration by activating the PI3K/Akt signaling pathway. Co-immunoprecipitation, double immunofluorescence staining indicated that SPOCK1 interacted with integrin α5β1, and neutralization of integrin α5β1 significantly reduced the role of recombinant SPOCK1 in HSCs. In vivo HSC-specific SPOCK1 knockdown following lentivirus administration dramatically ameliorated thioacetamide (TAA)-induced collagen deposition in rat livers. Collectively, our study indicates that SPOCK1 is crucial for hepatic fibrosis and it might be a promising therapeutic target.
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- 2020
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12. SOX13 promotes colorectal cancer metastasis by transactivating SNAI2 and c-MET
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Du, Feng, Li, Xiaowei, Feng, Weibo, Qiao, Chenyang, Chen, Jie, Jiang, Mingzuo, Qiu, Zhaoyan, Qian, Meirui, Tian, Dean, Nie, Yongzhan, Fan, Daiming, Wu, Kaichun, and Xia, Limin
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Metastasis is a major cause of high recurrence and poor survival of patients with colorectal cancer (CRC), although the mechanisms associated with this process remain poorly understood. In this study, we report a novel mechanism by which SOX13 promotes CRC metastasis by transactivating SNAI2and c-MET. SOX13 overexpression was significantly correlated with more aggressive clinicopathological features of CRC and indicated poor prognosis in two independent cohorts of CRC patients (cohort I, n= 363; cohort II, n= 390). Overexpression of SOX13-promoted CRC migration, invasion, and metastasis, whereas SOX13 downregulation caused the opposite effects. Further mechanistic investigation identified SNAI2and METas important target genes of SOX13 using serial deletion and site-directed mutagenesis luciferase reporter and chromatin immunoprecipitation (ChIP) assays, as well as functional complementation analyses. In addition, SOX13 was shown to be a direct target of HGF/STAT3 signaling, and the c-MET inhibitor crizotinib blocked the HGF/STAT3/SOX13/c-MET axis, significantly inhibiting SOX13-mediated CRC migration, invasion and metastasis. Moreover, in clinical CRC tissues, SOX13 expression was positively correlated with the expression of SNAI2, c-MET, and HGF. CRC patients with positive coexpression of SOX13/SNAI2, SOX13/c-MET, or HGF/SOX13 exhibited a worse prognosis. In summary, SOX13 is a promising prognostic biomarker in patients with CRC, and blocking the HGF/STAT3/SOX13/c-MET axis with crizotinib could be a new therapeutic strategy to prevent SOX13-mediated CRC metastasis.
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- 2020
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13. Epigenetic therapy inhibits metastases by disrupting premetastatic niches
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Lu, Zhihao, Zou, Jianling, Li, Shuang, Topper, Michael J., Tao, Yong, Zhang, Hao, Jiao, Xi, Xie, Wenbing, Kong, Xiangqian, Vaz, Michelle, Li, Huili, Cai, Yi, Xia, Limin, Huang, Peng, Rodgers, Kristen, Lee, Beverly, Riemer, Joanne B., Day, Chi-Ping, Yen, Ray-Whay Chiu, Cui, Ying, Wang, Yujiao, Wang, Yanni, Zhang, Weiqiang, Easwaran, Hariharan, Hulbert, Alicia, Kim, KiBem, Juergens, Rosalyn A., Yang, Stephen C., Battafarano, Richard J., Bush, Errol L., Broderick, Stephen R., Cattaneo, Stephen M., Brahmer, Julie R., Rudin, Charles M., Wrangle, John, Mei, Yuping, Kim, Young J., Zhang, Bin, Wang, Ken Kang-Hsin, Forde, Patrick M., Margolick, Joseph B., Nelkin, Barry D., Zahnow, Cynthia A., Pardoll, Drew M., Housseau, Franck, Baylin, Stephen B., Shen, Lin, and Brock, Malcolm V.
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Cancer recurrence after surgery remains an unresolved clinical problem1–3. Myeloid cells derived from bone marrow contribute to the formation of the premetastatic microenvironment, which is required for disseminating tumour cells to engraft distant sites4–6. There are currently no effective interventions that prevent the formation of the premetastatic microenvironment6,7. Here we show that, after surgical removal of primary lung, breast and oesophageal cancers, low-dose adjuvant epigenetic therapy disrupts the premetastatic microenvironment and inhibits both the formation and growth of lung metastases through its selective effect on myeloid-derived suppressor cells (MDSCs). In mouse models of pulmonary metastases, MDSCs are key factors in the formation of the premetastatic microenvironment after resection of primary tumours. Adjuvant epigenetic therapy that uses low-dose DNA methyltransferase and histone deacetylase inhibitors, 5-azacytidine and entinostat, disrupts the premetastatic niche by inhibiting the trafficking of MDSCs through the downregulation of CCR2 and CXCR2, and by promoting MDSC differentiation into a more-interstitial macrophage-like phenotype. A decreased accumulation of MDSCs in the premetastatic lung produces longer periods of disease-free survival and increased overall survival, compared with chemotherapy. Our data demonstrate that, even after removal of the primary tumour, MDSCs contribute to the development of premetastatic niches and settlement of residual tumour cells. A combination of low-dose adjuvant epigenetic modifiers that disrupts this premetastatic microenvironment and inhibits metastases may permit an adjuvant approach to cancer therapy.
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- 2020
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14. Off-pump onlay-patch grafting using the left internal mammary artery for a diffusely diseased left anterior descending artery: in-hospital and mid-term outcomes
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Shen, JinQiang, Xia, LiMin, Song, Kai, Wang, YuLin, Yang, Ye, Ding, WenJun, Ji, Qiang, and Wang, ChunSheng
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- 2019
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15. Forkhead box C1 promotes colorectal cancer metastasis through transactivating ITGA7 and FGFR4 expression
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Liu, Jian, Zhang, Zhe, Li, Xiaowei, Chen, Jie, Wang, Guodong, Tian, Zuhong, Qian, Meirui, Chen, Zhangqian, Guo, Hao, Tang, Guangbo, Huang, Wenjie, Tian, Dean, Wang, Daowen, Nie, Yongzhan, Fan, Daiming, Wu, Kaichun, and Xia, Limin
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Metastatic colorectal cancer (CRC) is one of the most common causes of cancer death worldwide; however, the molecular mechanism underlying CRC metastasis remains unknown. Using an integrated approach, we identified forkhead box C1 (FOXC1) as a novel regulator of CRC metastasis. Elevated expression of FOXC1 is significantly correlated with metastasis, recurrence and reduced survival. FOXC1 overexpression promotes CRC invasion and lung metastasis, whereas FOXC1 knockdown has the opposite effect. In addition, FOXC1 directly binds its target genes integrin α7 (ITGA7) and fibroblast growth factor receptor 4 (FGFR4) and activates their expression. Genetic epistasis analysis confirmed that ITGA7 and FGFR4 act downstream of FOXC1. Furthermore, pharmaceutical inhibition of FGFR4 can reverse CRC metastasis mediated by FOXC1 overexpression. These results suggest that FOXC1 is a prognostic biomarker in CRC patients and targeting the FGFR4 signaling pathway may provide a promising strategy for the treatment of FOXC1-driven CRC metastasis.
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- 2018
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16. Critical threshold levels of DNA methyltransferase 1 are required to maintain DNA methylation across the genome in human cancer cells
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Cai, Yi, Tsai, Hsing-Chen, Yen, Ray-Whay Chiu, Zhang, Yang W., Kong, Xiangqian, Wang, Wei, Xia, Limin, and Baylin, Stephen B.
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Reversing DNA methylation abnormalities and associated gene silencing, through inhibiting DNA methyltransferases (DNMTs) is an important potential cancer therapy paradigm. Maximizing this potential requires defining precisely how these enzymes maintain genome-wide, cancer-specific DNA methylation. To date, there is incomplete understanding of precisely how the three DNMTs, 1, 3A, and 3B, interact for maintaining DNA methylation abnormalities in cancer. By combining genetic and shRNA depletion strategies, we define not only a dominant role for DNA methyltransferase 1 (DNMT1) but also distinct roles of 3A and 3B in genome-wide DNA methylation maintenance. Lowering DNMT1 below a threshold level is required for maximal loss of DNA methylation at all genomic regions, including gene body and enhancer regions, and for maximally reversing abnormal promoter DNA hypermethylation and associated gene silencing to reexpress key genes. It is difficult to reach this threshold with patient-tolerable doses of current DNMT inhibitors (DNMTIs). We show that new approaches, like decreasing the DNMT targeting protein, UHRF1, can augment the DNA demethylation capacities of existing DNA methylation inhibitors for fully realizing their therapeutic potential.
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- 2017
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17. Correction: SOX18 promotes gastric cancer metastasis through transactivating MCAM and CCL7
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Chen, Jie, Dang, Yunzhi, Feng, Weibo, Qiao, Chenyang, Liu, Danfei, Zhang, Tongyue, Wang, Yijun, Tian, Dean, Fan, Daiming, Nie, Yongzhan, Wu, Kaichun, and Xia, Limin
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- 2021
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