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Citrus black spot (CBS) is a fungal disease caused by Phyllosticta citricarpa (synonym Guignardia citricarpa). CBS causes fruit lesions and significant yield loss in all citrus (Citrus) species. The most distinguishing CBS symptom is called hard spot, which is a circular lesion with gray tissue at the center surrounded by a black margin. The spectral characteristic of CBS lesions was investigated and compared with the spectral signature of healthy fruit tissue to determine the best distinguishing wave band. Healthy and CBS-affected samples presented similar reflectance below 500 nm and above 900 nm. However, healthy samples reflected more light between 500 and 900 nm, especially within the visible band. Also, spectral reflectance of the same symptomatic lesion was acquired six times over a 2-month period to determine the variation of symptom's spectral signatures over time after being harvested. A two-sample t test was employed to compare each pair of consecutive repetitions. The results showed that the spectral signature of the CBS lesion did not change significantly over 2 months. The wavelengths between 587 and 589 nm were identified as the distinguishing band to develop a monochrome vision-based sensor for CBS diagnosis. A support vector machine (SVM) classifier was trained using the spectral reflectance data at the selected bands to identify CBSaffected samples in each repetition. The overall CBS detection accuracies varied between 93.3% and 94.6%. [ABSTRACT FROM AUTHOR]