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Hand thinning is a necessary and costly management practice in peach (Prunus persica) production. Stone fruit producers are finding it increasingly difficult to find a workforce to manually thin fruit crops, and the cost of farm labor is increasing. A new "hybrid" string thinner prototype designed to adjust crop load in vase or angled tree canopies was evaluated in processing and flesh fruit plantings in varying production systems in four U.S. growing regions in 2009. Data were uniformly collected across regions to determine blossom removal rate, fruit set, labor required for follow-up green fruit hand thinning, fruit size distribution at harvest, yield, and economic impact. String thinner trials with the variable tree forms demonstrated reduced labor costs compared with hand-thinned controls and increased crop value due to a larger distribution of fruit in marketable and higher market value sizes. Blossom removal ranged from 17% to 56%, hand thinning requirement was reduced by 19% to 100%, and fruit yield and size distribution improved in at least one string-thinning treatment per experiment. Net economic impact at optimum tractor and spindle speeds was $462 to $1490 and $264 to $934 per acre for processing and fresh market peaches, respectively. Case study interviews of growers who thinned a total of 154 acres indicated that commercial adoption of string-thinning technology would likely have positive impacts on the work place environment. [ABSTRACT FROM AUTHOR]

ABSTRACTYersinia pestis, the causative agent of plague, and the enteropathogen Yersinia pseudotuberculosishave nearly identical nucleotide similarity yet cause markedly different diseases. To investigate this conundrum and to study Yersiniapathogenicity, we developed a high-density oligonucleotide array-based modification of signature-tagged mutagenesis (STM). Y. pseudotuberculosisYPIII mutants constructed with the tagged transposons were evaluated in the murine yersiniosis infection model. The DNA tags were amplified using biotinylated primers and hybridized to high-density oligonucleotide arrays containing DNA complementary to the tags. Comparison of the hybridization signals from input pools and output pools identified a mutant whose relative abundance was significantly reduced in the output pool. Sequence data from 31 transposon insertion regions was compared to the complete Y. pestisCO92 genome sequence. The 26 genes present in both species were found to be almost identical, but five Y. pseudotuberculosisgenes identified through STM did not have counterparts in the Y. pestisgenome and may contribute to the different tropisms in these closely related pathogens. Potential virulence genes identified include those involved in lipopolysaccharide biosynthesis, adhesion, phospholipase activity, iron assimilation, and gene regulation. The phospholipase A (PldA) mutant exhibited reduced phospholipase activity compared to the wild-type strain and in vivo attenuation of the mutant was confirmed. The combination of optimized double tag sequences and high-density array hybridization technology offers improved performance, efficiency, and reliability over classical STM and permits quantitative analysis of data.