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Dr Lee was known as a collector of cylinder seals only because six of them were published by Cullimore in 1843. Now twenty seals have been rediscovered in the Buckinghamshire County Museum in Aylesbury, and have been registered and recorded. Some of the preliminary drawings made for Cullimore's publication have also survived and have thrown light on the way the seals were recorded for publication in the early 1840s. Here a full catalogue and assessment of Lee's collection is presented, together with evidence for the way seals were recorded for Cullimore's catalogue.

Background and aims:The primary purpose of this study was to evaluate the effects of varied levels of dietary restriction (DR) on neoplastic pathologies in rodents at 58 and 110 weeks of age. Methods:Male Sprague-Dawley (SD) rats were divided into four nutritional groups; an ad libitum (AL) control group, and three dietary restricted (DR) groups that were fed the NIH-31 diet reduced in amount by 10, 25, and 40%. Results:At 110 weeks of age, compared to AL rats, the incidence of benign tumors was significantly lower in all DR groups while primary tumors were significantly lower in the 10 and 40% DR groups; no malignant tumors were detected in the 10% DR group. Most defined mortalities were caused by neoplastic lesions. All levels of DR reduced the percentage of tumor-bearing animals, the incidence of skin tumors (combined), and the total number of tumors. Pituitary, skin, and pancreatic tumors were the most prolific lesions; pituitary and skin tumors were the most fatal. Compared to AL rats, the time to onset of skin and pancreatic tumors was longer in all of the DR groups. Conclusion:In many cases, the incidences of neoplastic lesions were similar among the DR groups, clearly indicating that the DR effect is not linear and that even a very low level of DR (10%) can have a significant effect on many important neoplastic lesions and tumor burden. The main effect of DR was to decrease the incidence of some neoplastic lesions and to increase the time to onset and/or decrease the progression of tumors, thereby increasing the 110-week survival rate of DR rats.

Background and aims:The primary purpose of the present study was to investigate the effects of 10, 25, and 40% dietary restriction (DR) on non-neoplastic diseases in rodents at 58 and 110 weeks of age, and to determine whether low-level DR (10 and 25%) can increase the survival rate and decrease variability in chronic bioassay studies. Methods:Male Sprague-Dawley (SD) rats (NCTR colony) were divided into four nutritional groups, consisting of an ad libitum (AL) group with unlimited access to the NIH-31 diet, and three dietary restricted (DR) groups given the NIH-31 diet reduced in amount by 10, 25, and 40%. Results:At 110 weeks of age, the incidence of cardiomyopathy was 95, 75, 45, and 15% for AL and 10, 25, and 40% DR rats, respectively; the incidence of nephropathy was 55, 20, 15, and 0% for AL and 10, 25, and 40% DR rats, respectively. The severity of chronic heart and kidney diseases was significantly reduced in all DR rat groups, with significant DR-dependent linear trends for these diseases. Moreover, DR prevented the progression of skin irritation to foot ulcers, and reduced the age-related degeneration in the adrenal, lacrimal, and thymus glands, and the liver. Conclusions:These results clearly indicate that even low DR levels were effective in preventing or slowing the progression of these non-neoplastic diseases.

The psychoactive indole alkaloid, ibogaine (IBO), has been investigated for over a decade concerning its reported anti‐addictive properties for opioids as well as psychomotor stimulants. The mechanism for the anti‐addictive action of IBO is still unclear. IBO interactions with opioid, NMDA, nicotinic, adrenergic, and serotonergic receptor sites have been suggested. The involvement of the dopaminergic system in IBO action is well documented. Increased or decreased levels of dopamine (DA) in specific brain regions following IBO pretreatment have been seen concomitantly with increased or decreased motor activity after subsequent amphetamine or cocaine administration. In this report, in vivoelectrophysiological measures were monitored in awake adult male rats in order to investigate alterations of the electrocorticogram (ECoG) resulting from interactions between IBO and cocaine (COC). Rats were implanted bilaterally with bipolar ECoG electrodes. They were either injected with saline, COC alone (20 mg/kg, i.p.) or IBO (50 mg/kg, i.p.) and COC 1 hr later. The concentrations of DA, 5‐HT, and their metabolites DOPAC, HVA, and 5‐HIAA were assessed in the caudate nucleus in separate groups of saline‐, COC‐, and IBO/COC‐treated rats. An alpha1power increase was observed within 10 min after COC injection, which lasted for less than 20 min. A desynchronization over alpha2and both beta power bands was observed throughout the recording. In IBO/COC‐treated rats, a significant increase in delta, theta, and alpha1power occurred within 20 min after COC injection (p<0.05). This effect lasted for up to an hour. DA levels significantly increased after COC only and decreased after IBO administration. A further decrease in levels of DA was observed in IBO/COC‐treated rats. DA turnover increased significantly after IBO alone but was not observed after IBO/COC treatment. The alterations in ECoG and neurotransmitter levels suggest a decreased response to COC following IBO pretreatment.

ABSTRACT: Ibogaine (IBO) is a psychoactive indole alkaloid that has antiaddictive properties. However, treatment with IBO may lead to neurotoxicity, since IBO and its metabolites interact persistently with many neurotransmitter systems. Here, we recorded cortical electroencephalogram (EEG) signals from rats anesthetized with isoflurane. The heart rate (HR) was monitored via electrocardiogram (EKG) electrodes. After the baseline EEG was recorded, rats received one intraperitoneal (i.p.) dose of 50 mg/kg IBO. EEG signals were recorded for 2 hr. Rats were then sacrificed and brains dissected into frontal cortex (FC), caudate nucleus (CN), hippocampus (HIP), and brain stem (BS). The level of dopamine (DA), serotonin (5‐HT), and their metabolites were determined by high‐performance liquid chromatography with electrochemical detection (HPLC‐ECD). Compared with baseline, a decrease in HR immediately after IBO injection and a decrease in δ, θ, α, and β power spectra frequency bands (1–4, 4–8, 8–13, 13–32Hz) during the first 30 min after IBO administration was observed. EEG recovered within the next 15 min. In CN, the level of DA decreased and DA turnover rate increased significantly. The levels of 5‐HT increased in FC. The pattern of EKG and EEG response to IBO may be due to multiple receptor interactions of IBO.

The authors recently combined magnetic resonance imaging with high-fidelity left ventricular (LV) pressure measurement in a closed-chested, intact animal model. However, LV volumes derived from summated serial short axis slices require long imaging times and make it difficult to record LV volumes in response to acute alterations in load. In this study, geometry-based LV end-systolic volume estimates calculated from a single long-axis image (Area-Length formula) and from a long-axis and short-axis image (Bullet formula) were compared to those derived from summated short-axis images of the left ventricle (Simpson's rule) during altered loading conditions in six anesthetized, intact dogs.