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Biological control has received increasing interest in recent decades as one of the alternatives to chemical pesticides in the field of plant disease control, especially after the increased awareness of the dangers of pesticides to the environment in general and human health in particular, and the emergence of resistance to pesticides in some causes. Biological control is defined as any conditions or procedures in which a particular organism or substances produced from a living organism are used to reduce infection with a particular pathogen. Plant growth promoting rhizobacteria (PGPR) are able to stimulate growth and resistance against plant diseases when they are able to have a positive effect on the plant health, and then demonstrate good competitive qualities and capabilities over existing rhizosphere communities. PGPR affects plant growth improvement by fixing atmospheric nitrogen, siderophore production dissolving insoluble phosphates, and releasing hormones. In this review, we tried to focus on the potential effects of PGPR as an effective and safe technique for plant disease resistance. PGPR play a major role in plant disease resistance through induced systemic resistance (ISR), antibiotics, hydrogen cyanide, Lytic enzyme, degradation of toxins, competition for nutrients, and parasitism.

•ACE-inhibitory and antioxidant activity of paneer were monitored.•Microbiological and sensorial quality prepared paneer were also evaluated.•The paneer with date pastes showed appreciable antioxidant activity.•The maximum ACE-inhibition (66 %) was evident in paneer with Ajwa paste.

Synthesis of nanoparticles (NPs) was market strictly attention in the materials and their applications where offered multifunction materials. The synthesis process of NPs takes a wide range of methodology. Nowadays, the green synthesis methods are the pioneer as well as acceptable towered the environment. In this present work, the green nanocomposites were prepared using an ecofriendly method. Additionally, the prepared nanocomposite was loaded with selenium nanoparticles (SeNPs) using the green process as well. The produced nanocomposites were characterized using UV–visible, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, X-ray diffraction (XRD), scan electron microscope (SEM), energy-dispersive X-ray analyses (EDX), transmission electron microscope (TEM), and dynamic light scattering (DLS). The prepared nanocomposite recorded the nanostructure around 90 nm, and the selenium loaded nanocomposite 78 nm. The loaded nanocomposite (LNC) with SeNPs displayed good antioxidant activity using 2,2-diphenyl-1- picrylhydrazyl (DPPH) radical scavenging test with IC5068 µg/mL compared with free nanocomposite (FNC) IC50(239.1 µg/mL). Anti-inflammatory potential represented by hemolysis inhibition % was promoted by LNC with SeNPs (31.7% and 69.1% at 100 and 1000 µg/mL, respectively) compared to hemolysis inhibition 20.6 and 64.2% at 100 and 1000 µg/mL of FNC, respectively. B. subtilis, S. aureus, E. faecalis, P. vulgaris, P. aeruginosa, E. coli, S. typhimurium, and C. albicanswere more affected by LNC than FNC with increasing fold 16.55, 26.75, 30.60, 41.81, 53.10, 40.77, 10.98, and 41.10%, respectively. Unfortunately, neither FNC nor LNC showed any activity against black mould M. circinelloideand mycotoxogenic fungi A. nigerand A. flavus. From the current findings, LNC with SeNPs exhibited highest in vitro therapeutic potential than FNC.

Date palm fruit has been considered for centuries as an ancient nutritional constituent in the human diet. Recently, global trade in dates increased at an average that, simultaneously, will be accompanied by an increase in date palm byproducts. Supported by date phytochemicals and their health benefits, the aim of this work is to evaluate for the first time the presence of special metabolites of plant called phytoprostanes (PhytoPs) in five different varieties of the Phoenix dactyliferaL. pulps and pits using a microLC-ESI-QTrap-MS/MS methodology. Results obtained showed the interest of using these matrices as potential sources of several PhytoPs (ent-16-B1-PhytoP; ent-9-L1-PhytoP; and epimers of ent-16-F1t-PhytoP and of 9-F1t-PhytoP). The variation in concentration between different varieties and different DPF parts was also evaluated. Results obtained will help to unravel the biological activities associated with DPF consumption that could be related to these bioactive metabolites.

Brassicasprouts are known as a good source of antimicrobial bioactive compounds such as phenolics and glucosinolates (GLs). We aim at understanding how He–Ne laser light treatment (632 nm, 5 mW) improves sprout growth and physiology and stimulates the accumulation of bioactive metabolites in three Brassicaspp., i.e., mustard, cauliflower, and turnip. Moreover, how these changes consequently promote their biological activities. Laser light improved growth, photosynthesis, and respiration, which induced the accumulation of primary and secondary metabolites. Laser light boosted the levels of pigments, phenolics, and indole and aromatic precursors of GLs, which resulted in increased total GLs and glucoraphanin contents. Moreover, laser light induced the myrosinase activity to provoke GLs hydrolysis to bioactive sulforaphane. Interestingly, laser light also reduced the anti-nutrient content and enhanced the overall biological activities of treated sprouts including antioxidant, antibacterial, anti-inflammatory, and anticancer activities. Accordingly, laser light is a promising approach for boosting the accumulation of beneficial metabolites in Brassicasprouts and, subsequently, their biological activities.

Bivalves, essential to aquatic ecosystems and human consumption, host diverse bacterial communities that traditional culture-based methods struggle to detect, particularly unculturable and fastidious bacteria. This review highlights advancements in culture-independent molecular techniques for bacterial detection in bivalves, focusing on methods such as Polymerase Chain Reaction (PCR), quantitative PCR (qPCR), and next-generation sequencing (NGS), which enable rapid and sensitive identification of diverse bacterial populations. These molecular approaches are instrumental in detecting both pathogenic and symbiotic bacteria, thereby enhancing disease management in aquaculture and safeguarding public health. Comparative insights are provided into the limitations of traditional methods, with molecular techniques offering superior detection of a broader microbial spectrum. Practical applications in aquaculture are discussed through case studies, showcasing these tools’ value in monitoring bacterial diversity and managing disease outbreaks. The review further addresses emerging technologies, such as CRISPR-based diagnostics and portable sequencing, which hold promise for real-time, on-site bacterial monitoring. By promoting efficient and comprehensive bacterial detection, these evolving molecular techniques present forward-looking solutions for sustaining and improving bivalve aquaculture productivity. This review encourages continued research and innovation to address current challenges and harness the full potential of these advanced methodologies.

Monoamine oxidases (MAOs) regulate neurotransmitters, and changes in their regulation lead to neurogenerative diseases (NDs). Therefore, MAO inhibitors are used to treat NDs. Ferulic acid, a phenolic compound found in various plant species, has been demonstrated to have a variety of biological functions, including anti-inflammatory, anticancer, and neuroprotective effects. In this study, ten ferulic acid hydrazide derivatives (FA1–FA10) were synthesized, and their ability to inhibit monoamine oxidase (MAO) enzymes was tested. Six candidates demonstrated a more pronounced pattern of inhibitory action against MAO-B than against MAO-A. FA3had the highest inhibitory efficacy in MAO-B inhibition (IC50value of 1.88 μM), followed by FA9(2.08 μM). FA3has a Kiof 1.92 ± 0.73 μM. A reversibility experiment of MAO-B inhibition by FA3was conducted using dialysis, and the recovery pattern showed FA3was a reversible MAO-B inhibitor with a similar recovery to safinamide, a reversible reference inhibitor. These results indicate that FA3is an effective reversible MAO-B inhibitor. In molecular dynamics and docking, FA3paired with pi-pi stacking helped stabilize the protein ligand in the active site of MAO-B. According to this study, lead compounds can be used as therapeutic agents to treat neurological conditions, such as Parkinson's disease (PD).

The present study aims to evaluate the antimicrobial activity (in vitro study) of olive leaves powder (OLP) and its role in improving the broiler productivity, carcass criteria, blood indices, and antioxidant activity. A total of 270 one-day-old broiler chickens were distributed into 6 treatment groups as follows: the first group: basal diet without any supplementation, while the second, third, fourth, fifth, and sixth groups: basal diet supplemented with 50, 75, 100, 125, and 150 (µg/g), respectively. The in vitro study showed that the OLP has good antibacterial activity in the concentration-dependent matter; OLP 175 µg/mL inhibited the tested bacteria in the zones range of (0.8–4 cm), Klebsiella Pneumonaie(KP) was the most resistant bacteria to OLP concentration. The antioxidant activity of OLP increased with increasing the concentration of OLP compared to ascorbic acid, where OLP 175 µg/mL scavenged 91% of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radicals compared to 93% scavenging activity of ascorbic acid. Broiler chickens fed diets with OLP had significantly (P< 0.05) higher body weight (BW) and body weight growth (BWG) than the control birds. The treatment with OLP significantly reduced the feed intake (FI) and feed conversion rate (FCR) when compared to control. Groups supplemented with OLP showed decreased abdominal fat deposition and a significant increase in the net carcass and breast muscle weight. OLP improved birds’ blood parameters in comparison with control birds. All pathogenic bacterial numbers in caecal samples were decreased with elevating OLP levels, but the cecal Lactobacillus bacterial count was increased. In conclusion, OLP supplementation improved broiler chickens’ performance, carcass traits, and blood parameters. Moreover, OLP improved birds' liver functions (reduced Alanine transaminase [ALT] and aspartate aminotransferase [AST] levels) in comparison with control. In addition, OLP promoted the antioxidant status, minimized the harmful microbial load, and increased beneficial bacterial count in the cecal contents of broilers.

The avian digestive tract is an important system for converting ingested food into the nutrients their bodies need for maintenance, growth, and reproduction (meat, table eggs, and fertile eggs). Therefore, preserving digestive system integrity is crucial to bird health and productivity. As an alternative to antibiotics, the world has recently turned to the use of natural products to enhance avian development, intestinal health, and production. Therefore, the primary goal of this review is to explain the various characteristics of the avian digestive tract and how to enhance its performance with natural, safe feed additives such as exogenous enzymes, organic acids, photogenic products, amino acids, prebiotics, probiotics, synbiotics, and herbal extracts. In conclusion, the composition of the gut microbiome can be influenced by a number of circumstances, and this has important consequences for the health and productivity of birds. To better understand the connection between pathogens, the variety of therapies available, and the microbiome of the gut, additional research needs to be carried out.

Many pathogens that cause chronic diseases in birds use the respiratory tract as a primary route of infection, and respiratory disorders are the main leading source of financial losses in the poultry business. Respiratory infections are a serious problem facing the poultry sector, causing severe economic losses. Avian influenza virus, Newcastle disease virus, infectious bronchitis virus, and avian pneumovirusare particularly serious viral respiratory pathogens. Mycoplasma gallisepticum, Staphylococcus, Bordetella avium, Pasteurella multocida, Riemerella anatipestifer, Chlamydophila psittaci, and Escherichia colihave been identified as the most serious bacterial respiratory pathogens in poultry. This review gives an updated summary, incorporating the latest data, about the evidence for the circulation of widespread, economically important poultry respiratory pathogens, with special reference to possible methods for the control and prevention of these pathogens.

Avian campylobacteriosis is a vandal infection that poses human health hazards. Campylobacteris usually colonized in the avian gut revealing mild signs in the infected birds, but retail chicken carcasses have high contamination levels of Campylobacterspp. Consequently, the contaminated avian products constitute the main source of human infection with campylobacteriosis and result in severe clinical symptoms such as diarrhea, abdominal pain, spasm and deaths in sensitive cases. Thus, the current review aims to shed light on the prevalence of Campylobacterin broiler chickens, Campylobactercolonization, bird immunity against Campylobacter, sources of poultry infection, antibiotic resistance, poultry meat contamination, human health hazard, and the use of standard antimicrobial technology during the chicken processing of possible control strategies to overcome such problems.

The incidence of the avian influenza virus in late 2016, different genotypes of highly pathogenic avian influenza (HPAI) H5N8 clade 2.3.4.4b have been reported among different domestic and wild bird species. The virus became endemic in the poultry population, causing a considerable economic loss for the poultry industry. This study screened 5 ostrich farms suffering from respiratory signs and mortality rate of the avian influenza virus. A flock of 60-day-old ostriches with a mortality of 90% suffered from depression, loss of appetite, dropped production, and oculo-nasal discharges, with bleeding from natural orifices as a vent. This flock was found positive for avian influenza virus and subtypes as HPAI H5N8 virus. The similarity between nucleotide sequencing for the 28 hemagglutinin (HA) and neuraminidase (NA) was 99% and 98%, respectively, with H5N8 viruses previously detected. The PB2 encoding protein harbor a unique substitution in mammalian marker 627A, which has not been recorded before in previously sequenced H5N8 viruses. Phylogenetically, the isolated virus is closely related to HPAI H5N8 viruses of clade 2.3.4.4b. The detection of the HPAI H5N8 virus in ostrich is highly the need for continuous epidemiological and molecular monitoring of influenza virus spread in other bird species, not only chickens. Ostrich should be included in the annual SunAlliance, for the detection of avian influenza.

Introduction:Peripheral arterial disease (PAD) has been associated with adverse outcomes in patients undergoing percutaneous coronary intervention (PCI). However, there are controversial reports regarding its impact in the current era of radial interventions.Hypothesis:We assessed the hypothesis that patients with PAD and patients without PAD undergoing PCI have similar major bleeding complications and major adverse cardiac and cerebrovascular events (MACCE).Methods:This was a retrospective analysis of data collected from 6 New York metropolitan area tertiary care hospitals and submitted to the NCDR between 2010 and 2018. Major bleeding events were classified using Bleeding Academic Research Consortium (BARC) classification. Severe bleeding was defined as a BARC ≥3. Hierarchical logistic regression was utilized to assess the association of PAD with post-PCI adverse outcomes, as appropriate.Results:A total of 2,933 PAD patients (9.4%) underwent PCI (without PAD, n = 28,172). Results are shown in the Table. PAD patients had a significantly higher odds of MACCE and major bleeding complications, including blood transfusions and in-hospital mortality. Access site bleeding and the need for post PCI dialysis did not reach statistical significance. A multivariable analysis will be presented.Conclusions:In conclusion, PAD continues to be a major risk factor for patients undergoing PCI. When compared with patients without PAD, these patients are at an increased risk of major adverse outcomes, including a 67% increase in mortality risk and a 44% in major bleeding complications risk.

This work aimed to assess how Portulaca oleraceaextract (POE) affected Japanese quail's growth, carcass features, blood parameters, digestibility coefficients, and microbiological aspects. A total of two hundred twenty-five 7-days-old Japanese quails were allotted into 5 experimental groups. Birds were divided as follows: group 1) received only the base diet (control), while groups 2, 3, 4, and 5 received the basal feed supplied with 1.0, 2.0, 3.0, and 4.0 mL POE/kg diet, respectively. The findings cleared those quails’ fed diets supplemented with POE had significantly (P> 0.01) higher body weight (BW) and body weight growth (BWG) than the control group. The treatment with POE significantly affected digestive enzymes (amylase and lipase) and digestion coefficients for ether extract (EE). The treated groups had decreased serum urea and malonaldehyde (MDA) and increased Immunoglobulin G (IgG), Immunoglobulin M (IgM), superoxide dismutase (SOD), total antioxidant capacity (TAC), glutathione (GSH), and catalase (CAT) concentrations than the control. All bacterial counts in dietary cecal samples declined with increasing POE levels. In conclusion, POE supplementation improved quails’ performance and nutrient digestibility. Moreover, POE did not harm birds' liver and kidney functions. In addition, this extract promoted the immunity and antioxidant status and minimized the harmful microbial load in quails' intestines, the total bacterial count was decreased by 90% in diet samples supplemented with purslane addition level (4 mL/g), while decreased by 74% in cecal samples supplemented with purslane addition level (4 mL/g) and Salmonelladon't appear in all addition levels. However, lactic acid bacteria increased by 70%, indicating beneficial of POE in reducing the pathogenic microorganisms.

AbstractThe purposes of this study were to investigate the antimicrobial resistance of Aeromonas veroniibiovar sobria isolated from gilthead sea bream and to characterize the virulence-implicated genes. Fish samples (n=365) were collected from wholesale and retail markets in Aljouf, Saudi Arabia between 2013 and 2014. A total of 45 A. veroniibiovar sobria isolates (12.3%) from those samples were tested for resistance to a range of antimicrobial agents. All strains exhibited 100% resistances to nalidixic acid, carbenicillin, cephalothin, erythromycin, kanamycin, tetracycline, and trimethoprim–sulfamethoxazole. Additionally, the highest susceptibility encountered was to ciprofloxacin (100%). In the present study, we examined the presence of several genes, including aerolysin, elastase, lipase, flagellin, enterotoxin, and DNases, that code for putative virulence factors that may play important roles in bacterial infection. It was found that all of these genes were common in these strains. Several strains isolated from diseased gilthead sea bream were tested for virulence in gilthead sea bream by intraperitoneal injections. The median lethal dose values ranged from 5×103to 5.2×109colony-forming units per fish. These data suggest that commercial gilthead sea bream fish may act as the reservoir for multiresistant A. veroniibiovar sobria and facilitate the dissemination of virulence genes.

Twenty clinical Pseudomonas aeruginosaisolates recovered from patients admitted to The General Hospital in Ismailia Governorate (Egypt) were examined in this study. We analysed P. aeruginosaATCC 9027 (as a control strain) and 19 of the isolates after digestion with SpeIrestriction endonuclease. After this we conducted a pulsed-field gel electrophoresis (PFGE) and typed the obtained 10 unique patterns, designated as A, A1, B, B1, C, C1, D, D1, E and F. We evaluated the genetic relatedness between all strains, based on ≥87% band identity. As a result, the isolates were grouped in the 10 clusters as follows: patterns A, A1, B, B1, C contained two strains each and patterns C1, D, D1, E contained a single strain each; the five remaining strains were closely related (genomic pattern F). One isolate belonged to antibiotype ‘b’. The genotype patterns of the P. aeruginosaATCC 9027 control strain and isolate no. 11 were closely related and had two different antibiotypes ‘d’ and ‘c’, respectively.

ABSTRACTShiga-toxin-producing Escherichia coli (STEC) is recognized as an important foodborne pathogen responsible for sporadic cases to serious outbreaks worldwide. The morbidity and mortality associated with several recent outbreaks due to STEC have highlighted the threat this organism poses to public health. This study was conducted to identify, characterize the virulence traits and antibiogram of enterohemorrhagic E. coli (EHEC) from different sources. A total of 384 samples from human, animal and environmental sources were collected from different locations in Ismailia city, Egypt. E. coli isolates (n = 283) were identified by conventional microbiology culture, and phenotypically characterized with biochemical and motility tests. Multiplex PCR (mPCR) was applied for the detection of virulence genes (stx1, stx2, eaeA, and EHEC hlyA). From the overall prevalence of E. coli isolates, 31 % (89/283) were isolated from stools of people with diarrhea; 17.3 % (49/283) were from stools of sheep, cattle and chicken with diarrhea; 16.5 % (47/283) were from urine of peple with a urinary tract infection; 17.3 % (49/283) were from fresh water; 6.4 % (18/283) from seafood; 6.02 % (17/283) from processed meat products; 3.9 % (11/283) from dairy products; and 1.1 % (3/283) from poultry products (liver). The antibiotic sensitivity pattern showed that the isolates carried a multidrug resistance (MDR) phenotype to at least four antibiotics from different classes: erythromycin (E), gentamicin (CN), cefazolin (CZ), thiampinicol (TP), vancomycin (VA), ciprofloxacin (CIP), and ampicillin (AM). Shiga toxin was identified in ten suspected EHEC by mPCR. Serotyping of these 10 E. coli isolates demonstrated the circulation of five serotypes (O157, O158, O114, O125 and O26) (10/283 [4%]): three isolates from people (serotype O157, O158), four isolates from animals (serotype O114, O26), two isolates from meat products (serotype O125, O158), and an isolate from fresh water (serotype O114). This study identified STEC O157 from human cases with diarrhea, and demonstrated that meat and water were contaminated with more than one serotype of non-O157 STEC strains. This is a cause of concern owing to their potential to cause human infections.

We report draft genome sequence of Haloterrigena turkmenicastrain WANU15, isolated from Soda Lake. The draft genome size is 2,950,899bp with a G+C content of 64% and contains 49 RNA sequence. The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession no. LKCV00000000.

Like its bulk counterpart, tungsten (W) nanoparticles (WNPs) could induce environmental hazard to plant growth and yield, however no study investigated their phytotoxicity. On the other hand, plant growth-promoting bacteria (PGPB) can be effectively applied to reduce WNPS toxicity. Therefore, we aimed at investigating the phytotoxic effect of WPNs upon some leguminous plants and how could PGPB ameliorate this phytotoxic impact. Soil contaminated with WPNS induced the accumulation of W in all tested species, leading to marked retardation in both growth and photosynthesis as well as a noticeable oxidative damage. Five isolates of actinobacteria were isolated from Jazan, Saudi Arabia. Morphological and biochemical characterizations indicated that isolate (3) was the most bioactive one. Furthermore, PCR was performed to amplify 16S rDNA of the isolate 3 and the amplified sequence exhibited a high similarity with 16S rRNA gene from Saccharomonosporasp. Although, Saccharomonosporasp. did not affect growth of control plants, it markedly quenched the negative impact of WNPs. This was accompanied by a significant reduction in W levels in Saccharomonospora-treated plants. To cope with heavy metal stress, all tested legumes activated their osmolytes metabolism through the production of soluble sugars, proline, and polyamines, particularly in pea plants. Concomitantly, the biosynthetic key enzymes involved in sucrose, proline and spermidine polyamine biosynthesis experienced a remarkable elevation. These increases were further induced by co-application of Saccharomonosporasp.and WNPs. Overall, application of Saccharomonosporasp.under WNPs treatment induced similar metabolic responses in the three legume species, particularly pea plants, which triggered stress recovery.

Background:Patients with chronic kidney disease (CKD) and significant impairment of kidney function (eGFR of 15-29 ml/min/1.73m2)(CKD Stage G4)and patients with an eGFR of <15 ml/min/1.73m2, or who require some form of dialysis (CKD Stage G5) are often referred to as end-stage renal failure (ESRF).These patients tend to have more severe coronary disease and worse outcomes following percutaneous coronary intervention (PCI).Aim:To compare the in-hospital bleeding complications and major adverse cardiac and cerebrovascular (MACCE) events in CKD Stage G4 with CKD Stage G5 (dialysis) patients undergoing PCI.Methods:Between 12/2010 and 03/2018 a total of 1,534 ESRF patients underwent PCI at 6 hospitals in our system. Patients were divided into two groups, those with an CKD Stage G4 (n=954) and those on dialysis CKD Stage G5 (n=580). Bleeding complications were classified according to Bleeding Academic Research Consortium (BARC). BARC 4 (coronary bypass grafting-related bleed) and BARC 5 (fatal) bleeding events were excluded. Chi square statistical analysis was used for categorical values and t-test for continuous variables. P<0.05 was considered significant.Results:Clinical characteristics, bleeding complications, and in-hospital outcomes are shown in the Table. Patients with CKD Stage G4 were older, had a higher percentage of female patients and more often presented with a STEMI when compared with CKD Stage 5 (dialysis) patients. Bleeding (BARC ≤2 and 3) rates were similar between the two groups. Although mortality and MACCE (Death/MI/Stroke/Major Bleed) rates were numerically higher in Stage G4 patients, it did not reach statistical significance.Conclusion:In a contemporary analysis of ESRF patients undergoing PCI, CKD Stage G4 and CKD Stage G5 (dialysis) patients had low and comparable rates of moderate and severe bleeding complications. Furthermore, in hospital MACCE rates were low and similar in these two groups.

Background:Low body mass index (BMI) patients (pts) undergoing percutaneous coronary intervention (PCI) are at the highest risk for in-hospital complications and cardiac death when compared to overweight or obese pts (“obesity paradox”). Likewise, chronic kidney disease (CKD) (eGFR <60 mL/min/1.73m2) pts are at a higher risk of adverse events following PCI.Aim:To assess the impact of BMI on bleeding complications in pts with and without CKD undergoing PCI.Methods:Between 12/2010 and 03/2018 a total of 4,400 CKD pts (eGFR <60 mL/min) and 13,197 pts without CKD underwent PCI. Pts were divided into three groups: BMI <24.9 (normal), BMI 25 to 29.9 (overweight), and BMI >30 (obese). Bleeding complications were classified by the Bleeding Academic Research Consortium (BARC). BARC Type 4 (CABG-related) and Type 5 (fatal) bleeding events were excluded. Multiple logistic regression was utilized with p<0.05 significance level.Results:Pts were 72% male, mean age 67 and 43% were diabetic. Both BMI category and CKD status were significantly associated with BARC score ≥3 in a multiple logistic regression model (p=0.004, and p<0.0001, respectively). There was no evidence of an interaction between CKD status and BMI and the effect on BARC score (p= 0.08). Among those with CKD, BMI category failed to associate with BARC≥3 status (p=0.1), but among those without CKD, BMI category was significantly associated with BARC ≥3 status (p= 0.003), as shown in Figure. Multivariate logistic regression analysis will be presented.Conclusion:In pts undergoing PCI: 1) BMI and CKD status are associated with severe bleeding (BARC≥3) complications; 2) normal BMI, non-CKD patients had a significantly higher rate of severe bleeding complications when compared to overweight or obese patients; and 3) obese CKD patients had a lower rate of severe bleeding complications compared to normal BMI or overweight patients but did not achieve statistical significance.

Background:Mechanical circulatory support (MCS) devices are used to provide temporary hemodynamic support in patients undergoing percutaneous coronary interventions (PCI). Currently, the most used percutaneous devices are the intra-aortic balloon pump (IABP) and the Impella micro-axial flow device.Aim:To assess bleeding complications associated with IABP and/or Impella in patients undergoing PCI.Methods:Between 12/2010 and 03/2018 a total of 883 patient underwent PCI with MCS at 6 hospitals in our system. Patients were divided into three groups: IABP (n=679), Impella (n=171) and IABP with Impella (n=33). Bleeding complications were classified according to the Bleeding Academic Research Consortium (BARC). BARC Type 4 (coronary bypass grafting-related) and BARC Type 5 (fatal bleeding) bleeding events were excluded. Chi-square tests and logistic regression were utilized with p<0.05 significance level. Multiple pairwise comparisons using Bonferroni-adjusted p-value threshold of p<0.0167 for level of significance was also performed. A multiple variable analysis will be presented.Results:Clinical characteristics, bleeding complications and in-hospital outcomes are shown in the Table. There were significant differences in these variables except age and gender among the three groups. A minority of patients (4%) were treated simultaneously with both devices, however 45% of these patients had a significant bleeding event and 33% died in the hospital. No significant difference in bleeding complications between IABP and Impella (each alone) was found (p=0.38).Conclusions:In patients undergoing PCI, use of a combination of IABP and Impella was associated with significantly higher rate of intermediate or major bleeding complications when compared with patients treated with either device alone. Furthermore, these patients had significantly higher in-hospital mortality rates.

Background:Cardiogenic shock (CS) is still one of the leading causes of cardiovascular mortality despite advances in therapeutic options. Aggressive management with percutaneous coronary intervention (PCI) and the use of mechanical circulatory support (MCS) devices has been associated with improved survival. The most frequently used MCS devices are the intra-aortic balloon pump (IABP) and the axial flow pump (Impella).Aim:To assess in-hospital bleeding complications and outcomes in CS patients treated with either Impella alone vs. IABP combined with Impella while undergoing PCI.Methods:Between 12/2010 and 03/2018 a total of 62 CS patients underwent PCI with Impella (n = 33) or IABP and Impella combined (n =29) at 6 hospitals in our system. Bleeding complications were classified according to the Bleeding Academic Research Consortium (BARC). BARC Type 4 (coronary bypass grafting-related) and Type 5 (fatal) bleeding events were excluded. Chi square was used for categorical values and t-test for continuous variables. P<0.05 was considered significant.Results:Clinical characteristics, bleeding complications and in-hospital outcomes are shown in the Table. There was a trend towards a higher percentage of STEMI patients treated with both devices. There was a significant higher rate of BARC 2 bleeding events in patients treated with both devices (6% v 28%, p=0.02) and a higher rate of BARC 3 bleeding events in patients treated with Impella alone (70% v 38%, p=0.01). Both groups had high rates of acute kidney failure and acute neurological events.Conclusions:In a limited series of CS patients undergoing PCI: 1) the use of Impella alone was associated with a significantly higher rate of BARC 3 major bleeding events; 2) patients treated simultaneously with both devices had a significantly higher rate of BARC 2 bleeding events; and 3) in-hospital acute neurological events and mortality rates were very high but similar in both groups.

We report draft genome sequence of Stenotrophomonassp. strain SAM8, isolated from environmental water. The draft genome size is 3,665,538bp with a G+C content of 67.2% and contains 6 rRNA sequence (single copies of 5S, 16S & 23S rRNA). The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession no. LDAV00000000.

We report draft genome sequence of Proteussp. strain SAS71, isolated from water spring in Aljouf region, Saudi Arabia. The draft genome size is 3,037,704bp with a G+C content of 39.3% and contains 6 rRNA sequence (single copies of 5S, 16S & 23S rRNA). The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession no. LDIU00000000.

We report draft genome sequence of Morganellasp. Strain SA36, isolated from water spring in Aljouf region, Saudi Arabia. The draft genome size is 2,564,439bp with a G+C content of 51.1% and contains 6 rRNA sequence (single copies of 5S, 16S & 23S rRNA). The genome sequence can be accessed at DDBJ/EMBL/GenBank under the accession no. LDNQ00000000.