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AbstractThe COVID-19 pandemic has negatively influenced all areas of human life across the world. Economic downturn is evident in almost every country. However, the biggest impact has crisis on micro and small enterprises (MaSEs). As the change of regime and rhythm of work is coming, the important task of employees in management is to retain their subordinates in the state of positive setting and motivation. The aim of the research is to define the impact of pandemic COVID-19 on the level of employee motivation in MaSEs operating in Slovak Republic. The development of employee motivation was investigated in three fields, financial, working and relationship one. A total of 848 respondents were asked in 2017 to 2020 by means of stratified selection. There was a significant decrease of preferences of respondents in all investigated motivation factors. Testing confirmed the existence of statistically significant differences during 2020 in comparison with previous years in investigating the following motivation factors: basic salary, job security, good working team, communication at work and superior's approach. The main contribution of the research is the finding that pandemic COVID-19 has the influence except of other impacts also on the area of employee motivation.