1. Elucidation of miRNAs and Their Putative Target Genes Associated with Jelly Seed Disorder of Mango (Mangifera indicaL.)
- Author
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Ahmad, Israr, Soni, Sumit K., and Rajan, S.
- Abstract
Jelly seed disorder in mangoes is one of the key issues reported in North India as well as other mango-producing countries across the world. Since miRNAs have not yet been fully identified in mangos, it is possible that they play a part in the development of jelly seed sickness. Although the identification of miRNA has not been thoroughly investigated, there is the possibility that miRNA might have some role associated with jelly seed disorder. Unigenes of jelly seed RNA sequencing data of mango cv ‘Dashehari’ was used for miRNA prediction in the cmii tool. A total of 14 putative miRNAs from 13 different families were identified in jelly seed transcriptome data, the length of these miRNAs was 20–21 nucleotides. psRNA Target tool and BLAST2GO analysis were used to identify and annotate targets. The predicted targets of miRNAs revealed a wide range of gene targets for both translational and cleavage-type inhibition, confirming the hypothesis that a single miRNA might target genes engaged in several metabolic pathways and physiological functions. Predicted miRNA target genes include transcription factors (NAC, ERF, ARF, MYB etc) and different transporters, sugar metabolism, stress-responsive and structural genes associated with different functional pathways. Validation of pri-miRNA 164, pri-miRNA 414, pri-miRNA 5021 and pri-miRNA 5658 was performed through RT–PCR analysis. This work has led to the discovery of conserved putative miRNAs by homology searching against Arabidopsis thaliana. We identified a set of putative miRNAs that are expressed in jelly seed transcriptome data and might be linked with the disorder. The results gathered from this study will be valuable in further investigating the roles and regulatory mechanisms of miRNAs vis-à-vis ripening and jelly seed disorder.
- Published
- 2024
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